• KOREAN JOURNAL OF CROP SCIENCE
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• v.27 no.1
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• pp.72-77
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• 1982

To develop a new propagation method of Korean ginseng (Panax ginseng C.A. Meyer) by cutting, stem cuttings with leaflets obtained from ginseng seedlings were planted in rooting media treated with three levels of NAA concentration. NAA solution of 1.0 ppm was the most effective for the rooting of ginseng stem cuttings as well as for root growth after initiation of the roots from cuttings. A mixture of sand and leaf compost as a rooting medium for ginseng stem cuttings showed the best results in rooting percentage of cuttings and root growth after rooting. An acril film cap covering was very effective to prevent water loss by transpiration from the leaflets of stem cuttings and for rooting and root growth after rooting. Cuttings of leaf petiole with 5 leaflets from 2 year old ginseng plants showed good rooting as well as root growth after rooting in a mixture of sand and leaf compost treated with 1.0 ppm NAA.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.177-186
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• 2011

This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing $60{\mu}M$ ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: $1.5mg\;L^{-1}$ 1-naphthalene acetic acid (NAA), $1.5mg\;L^{-1}$ indole-3-butyric acid (IBA) and $1mg\;L^{-1}$ IBA with $0.5mg\;L^{-1}$ NAA (MIX). The effect of the photon flux ($120mmol\;m^{-2}\;s^{-1}$ and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

• Journal of Korea Foresty Energy
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• v.21 no.1
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• pp.32-40
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• 2002

This study was carried out to investigate the effects of rootball media and covering materials for air-layering on the rooting and growth responses of miniature tree material. The experiment was performed with Carpinus coreana Nakai in the vinyl-house condition. The covering material, jute tape increased the diameter growth at 1cm above the girdling part of the tree material. The combined use of the soil mixture of peatmoss and perlite(2:1, by volume) as rootball medium and jute tape increased the dry weight of current year leaves and shoots. The number of shoot shorter than 10cm and that of leaves from the shoot were produced more by using a sphagnum moss for rootball medium than the soil mixture. The use of jute tape shortened the period required for rooting and increased the number of fine roots and the total root dry weight at girdling part of the tree material. On the other hand, the number of long roots was produced more by using the sphagnum moss than the soil mixture.

• Journal of agriculture & life science
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• v.44 no.6
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• pp.39-44
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• 2010

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

• Journal of Plant Biotechnology
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• v.48 no.1
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• pp.54-61
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• 2021

This work describe an efficient method for the shoot induction and plant regeneration of seedling-derived apical bud explants of Tilia mandshurica Rupr. & Maxim. The highest rate of shoot induction (82.2%) was obtained when apical bud explants from juvenile seedlings (5 months old) were cultured on Murashige and Skoog (MS) medium containing 1.0 mg/L 6-benzylaminopurine (BAP). However, apical bud explants obtained from mature trees (12 years old) did not produce any shoots, even with BAP supplementation. Among the three cytokinins tested for shoot multiplication (BAP, zeatin, and kinetin), BAP was the most effective; the highest number of shoots per explant (2.1) was observed on MS medium supplemented with 1.0 mg/L BAP. In contrast, the longest average shoot length (3.0 cm) was observed after growth on MS medium with 2.0 mg/L zeatin. No multiplication occurred when apical bud explants were cultured with kinetin-supplemented media. During rooting of in vitro-elongated shoots, the highest rooting rate (100%) was observed in half-strength MS medium supplemented with 0.5 ~ 1.0 mg/L indole-3-butyric acid (IBA) or 3.0 mg/L 1-naphthaleneacetic acid (NAA). During the acclimatization process, plantlets that were rooted on the IBA (0.5 mg/L)-supplemented medium had the highest survival rate (100%) and maximum root length (18.5 cm). These findings suggest that a low concentration (0.5 mg/L) of IBA is appropriate for the rooting and acclimatization of T. mandshurica. Plants were successfully transferred to the greenhouse with a 100% survival rate. This protocol will be useful for the large-scale propagation of Tilia species.

• Horticultural Science & Technology
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• v.30 no.1
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• pp.13-20
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• 2012

This study was carried out to examine the effect of cutting condition on the growth of rooted cuttings and their subsequent growth of cut flower in plug cutting of Dendranthema grandiflorum 'Baekma'. The more leaves were attached to the cutting, the higher shoot growth of rooted cutting was observed. Cutting with two to six leaves was effective in shoot growth and rooting than any other treatment. Shoot growth of rooted cutting was not affected by cutting length, but rooting was better in 5 to 7 cm long cutting. Shoot growth and rooting of rooted cutting was promoted by increasing the cutting diameter, and rooting was better in 3.6-4.2 mm thick cutting than 3.1 mm thick cutting. As the treating concentration of NAA increased, shoot growth was inhibited and root length shortened in rooted cutting. Soaking with $100mg{\cdot}L^{-1}$ ${\alpha}$-naphtalene acetic acid (NAA) for 1 h was effective in shoot growth and rooting. Root growth such as root number, root length, and root weight was better in rooting medium mixed with 2:2 of peatmoss:perlite than the other treatments. When rooted cutting produced from cutting with four or six leaves was planted, better length, weight, and leaf number of cut flower was observed. In rooted cutting produced with 7-9 cm long cutting, growth of cut flower such as length, weight, and ray flower number was more effective than the others. Therefore, it is recommended that the 7 cm long and 3.6 mm thick cutting with four to six leaves is used to improve their rooting and subsequent growth of cut flower in plug cutting of Dendranthema grandiflorum 'Baekma'.

• Horticultural Science & Technology
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• v.19 no.3
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• pp.325-328
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• 2001

This experiment was conducted to investigate the effect of plug cell volume and medium on rooting and growth of lateral shoot cuttings of a few tomato cultivars. Plug cell volume was varied from 23 to 300 mL or control (cutting bed) and media used were carbonized rice hull (CRH), CRH+perlite, CRH+peatmoss and perlite+peatmoss. Nursery plants were able to be transplanted in 15 to 20 days after lateral shoots cutting in tomato. In volume of cutting media, the pots of 23 to 300 mL were proper, although root growth was gradually limited in decreased media volume. Rooting and growth was not influenced by plug tray cell medium of 120 mL or more. Cherry tomato Pepe (c.v.) showed 100% rooting and better growth, while in Momotaro (c.v.) rooting was 90%. There were 100% rooting and no considerable changes grown in all media tested of cutting plug tray volume of 30 mL.

• Journal of Plant Biotechnology
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• v.6 no.1
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• pp.39-43
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• 2004

A successful, efficient system for multiple shoot induction and plant regeneration of soybean (Glycine max) was established. Four soybean genotypes were compared for organogenic responses on various media cultured under light conditions. The adventitious shoots (98%, 2.6 shoots/cotyledon) directly from one-day-old cotyledon after germination induced by the hormone treatment and its efficiency was higher than any other conditions. The optimal medium for the induction of multiple shoots from cotyledon in Pungsannamulkong(shoot formation rate, 98%), Lx 16 (83%) and IIpumgeomjeongkong(63%) was MS medium supplemented with 2 mg/L BAP, but for Alchankong(75%), MS medium supplemented with 1mg/L zeatin and 1mg/L IAA, 3% sucrose, 4% Phytagel. Higher root induction (88%) was observed from the shoots placed on rooting medium (hormone-free MS basal). Plantlets were transferred onto the same medium supplemented with 1% activated charcoal for further development. With this treatment, regenerated plantlets were obtained within 7-8 weeks (shoot induction for 4 weeks, rooting and shoot elongation for 3-4 weeks).

• Journal of Korean Society of Forest Science
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• v.86 no.3
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• pp.391-398
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• 1997

Present study describes a method on the application of efficient tissue culture systems for the micro-propagation of juvenile and mature sawtooth oak(Quercus acutissima). Nodal segments with axillary buds were used as initial explant sources. WPM(Woody Plant Medium) was the best in growth and proliferation of shoot among the media tested. Although the single effect of zeatin revealed on two dorminant shoot elongation with normal growth until the elevation of levels up to 3.0mg/l, BAP($N^6$-benzyl amino purine) usually showed better response than zeatin on shoot multiplication and/or elongation. In addition, the incorporation of BAP and zeatin onto the culture media represents more effectiveness in shoot proliferation and its growth. Optimum concentrations of BAP and zeatin were 0.5 and 0.05~1.0mg/l, respectively. Ninety percent of the proliferated shoots was rooted on half-strength GD (Gresshoff and Doy, 1972) medium containing 0.5mg/l IBA(indole butyric acid) in 4 weeks after culture. More than 70% of the rooted plantlets survived after 5 months of transplanting into artificial soil mix containing equal amount of peatmoss and perlite. Among 27 plus tree clones which were grafted twice onto the juvenile rootstocks, only 4 clones revealed the possibility for shoot multiplication through tissue culture system. The capacity for the micropropagation using mature explant sources was highly depended on clonal differences compared with those of octet age. More than 90% of rooting ratio was obtained from the best responding clone. Among the 7 rooting media tested, GD medium was the best far rooting. The most effective rooting was obtained on half-strength GD medium containing 0.2 to 2.0mg/l IBA. More than 60% of rooted plantlets survived after 5 months of transplanting into the artificial soil mix.

• Korean Journal of Plant Tissue Culture
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• v.22 no.6
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• pp.335-337
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• 1995

The effect of plant growth regulators on proliferation of shoot from stem node culture of Japanese yew (Taxus cuspidata Sieb. et Zucc.) was studied using Quoirin and Lepoivre (1977) medium. Among the cytokinin tested, BAP, kinetin, and thidiazuron at various concentrations had no effect on shoot multiplication However when zeatin at 5$\times$10$^{-5}$ M was added to the medium, an average of 6 shoots were regenerated per explant after 8 weeks of culture. The ratio of rooting ex vitro was remarkably increased up to 34% by dipping the basal end in 0.5 to 1.0% IBA on talc compared with 3% in vitro rooting. Rooted plantlets were acclimated in greenhouse conditions for one month and successfully transplanted to the field.