Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Factors Affecting Organogenesis from Mature Cotyledon Explants and Regeneration in Soybean

  • Kim, Young Jin (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Park, Tae Il (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Kim, Hyun Soon (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Park, Ho Ki (Honam Agricultural Research Institute, National Institute of Crop Science) ;
  • Chon, Sang Uk (Biotech. Indust. Center, Dongshin Univ.) ;
  • Yun, Song Joong (Faculty of Biological Resources Science, and Institute of Agricultural Science and Technology, Chonbuk National University)
  • 발행 : 2004.03.01
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초록

A successful, efficient system for multiple shoot induction and plant regeneration of soybean (Glycine max) was established. Four soybean genotypes were compared for organogenic responses on various media cultured under light conditions. The adventitious shoots (98%, 2.6 shoots/cotyledon) directly from one-day-old cotyledon after germination induced by the hormone treatment and its efficiency was higher than any other conditions. The optimal medium for the induction of multiple shoots from cotyledon in Pungsannamulkong(shoot formation rate, 98%), Lx 16 (83%) and IIpumgeomjeongkong(63%) was MS medium supplemented with 2 mg/L BAP, but for Alchankong(75%), MS medium supplemented with 1mg/L zeatin and 1mg/L IAA, 3% sucrose, 4% Phytagel. Higher root induction (88%) was observed from the shoots placed on rooting medium (hormone-free MS basal). Plantlets were transferred onto the same medium supplemented with 1% activated charcoal for further development. With this treatment, regenerated plantlets were obtained within 7-8 weeks (shoot induction for 4 weeks, rooting and shoot elongation for 3-4 weeks).

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참고문헌

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