농업생명과학연구 (Journal of agriculture & life science)

  • 제44권6호
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  • Pages.39-44
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  • 2010
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  • 1598-5504(pISSN)
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  • 2383-8272(eISSN)
경상대학교 농업생명과학연구원 (Institute of Agriculture & Life Science, Gyeongsang National University)
권호 표지

Rapid Micropropagation by Axillary Buds Cultures of Smilax china

  • Song, Hyun-Jin (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.)) ;
  • Sim, Seon-Jeong (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.)) ;
  • Jeong, Mi-Jin (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.)) ;
  • Heo, Chang-Mi (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.)) ;
  • Kim, Hak-Gon (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.)) ;
  • Jeong, Gwon-Yong (Uiryeong-Gun Agricultural Technology Center) ;
  • Heo, Su-Yeoung (Uiryeong-Gun Agricultural Technology Center) ;
  • Choi, Yong-Weon (Uiryeong-Gun Agricultural Technology Center) ;
  • Park, Geun-Hye (Uiryeong-Gun Agricultural Technology Center) ;
  • Yang, Jae-Kyung (Uiryeong-Gun Agricultural Technology Center) ;
  • Moon, Hyun-Shik (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.)) ;
  • Choi, Myung-Suk (Div. of Environ. Forest Sci., Gyeongsang National Uni, (Insti. of Agric. of Life Sci.))
  • 투고 : 2010.10.01
  • 심사 : 2010.12.22
  • 발행 : 2010.12.31
⟨ 이전 논문 다음 논문 ⟩

초록

An efficient method for the rapid propagation of Smilax china from axillary buds was established. Plants with thick leafage were selected from Korea native S. china population. Axillary buds of S. china collected from selected plant and were cultured in various culture media (2MS, MS, 1/2MS, WPM, B5 and SH medium). Shoot was induced from axillary bud on MS basal medium after 4 weeks of culture. 1/2MS medium showed a higher growth rate than those of the others, while the lowest shoot growth was obtained in 2MS medium. Among the sucrose concentrations, 5% sucrose was the optimum level for shoots growth from axillay buds. Among cytokinins, $0.5mgL^{-1}$ 6-benzylaminopurine (BAP) treatment showed the best performance on shoot multiplication, yielding average shoot multiplication forming about 2.4. Rooting was induced directly near the base of the shoot on 1/2MS medium containing with three-auxins ${\alpha}-napthalene$ acetic acid (NAA), indole acetic acid (IAA) and ${\beta}-indolebutyric$ acid (IBA) (0.5 and $1.0mgL^{-1}$). The $1.0mgL^{-1}$ IBA treatments induced earliest rooting with maximum of root number and root growth. These rooted plantlets were successfully transferred to pots for 4 weeks hardening process, and were transferred to soil with above 90% survival rate.

키워드

과제정보

연구 과제 주관 기관 : Rural Development Administration

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