• Biomolecules & Therapeutics
• /
• 제8권3호
• /
• pp.207-212
• /
• 2000

The root extract of pulsatilla koreana has been used as antibacterial, antiparasitic and anti-inflam-matory analgesic agents in Traditional Medicine in Korea. Thus anti-inflammatory and analgesic actions of the methanol and water extracts of the root were investigated by administration in oral and intravenous route. From the results, it is concluded that the extracts exhibited the potent anti-inflammatory and analgesic actions in intravenous administration, but did not show the actions in oral administration in animals.

• Archives of Pharmacal Research
• /
• 제27권9호
• /
• pp.915-918
• /
• 2004

By bioassay-guided separation, an already known saponin, Pulsatilla saponin D was isolated from the root of Pulsatilla koreana Nakai as a antitumor component when evaluated by in vivo antitumor activity as well as in vitro cytotoxic activity test. It showed potent inhibition rate of tumor growth (IR, 82％) at the dose of 6.4 mg/kg on the BDF1 mice bearing LLC cells.

• Applied Biological Chemistry
• /
• 제41권2호
• /
• pp.191-196
• /
• 1998

할미꽃 $(Pulsatilla\;koreana\;N_{AKAI})$ 뿌리인 백두옹의 MeOH 추출물이 세균과 효모 등의 미생물에 대해 항미생물 활성을 나타내자 백두옹에 함유된 항미생물 활성물질의 탐색을 시도하였다. MeOH 추출물을 생물검정법을 지표로 solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography 등으로 정제하고, HPLC에 의해 활성물질을 분리하였다. 분리된 두 활성물질은 MS, $^{1}H-NMR$, $^{13}C-NMR$에 의해 4-hydroxy-3-methoxycinnamic acid와 3,4-dihydroxycinnamic acid로 동정되었다.

• 생약학회지
• /
• 제42권1호
• /
• pp.6-8
• /
• 2011

'BaegDuOng(白頭翁)' is one of the Chinese crude drugs used mainly as a pruritus, arthritis, cough and abdominal pain. The botanical origin of the crude drug has never been studied pharmacognostically. To clarify the botanical origin of 'BaegDuOng', the morphological and anatomical characteristics of Pulsatilla species growing in Korea, i.e. Pulsatilla koreana Nakai and P. cernua (Thunb.) Spreng. were studied. As a result, BaegDuOng from Korea was proved to be the root of Pulsatilla koreana.

• Natural Product Sciences
• /
• 제20권4호
• /
• pp.281-284
• /
• 2014

In this study, twenty-one oleanane-type triterpenoid saponins were isolated from a methanol extract of the roots of Pulsatilla koreana. Antagonistic activities were measured in these compounds by the aequorin based cellular functional assay system for the corticotropin releasing factor receptor (CRF1). Of them, compounds 7 - 10 showed the highest degree of CRF1 inhibition further at the concentration of $10{\mu}M$. Moreover, by the analysis based on the structure-activity relationship of isolated saponins, a sugar chain at C-3 and a carboxyl group at C-28, as well as a methyl group at C-23 seems to be key functional elements. To our knowledge, this is the first report on CRF1 inhibition of saponins from P. koreana.

• 생약학회지
• /
• 제31권2호
• /
• pp.174-184
• /
• 2000

From our previous report, the water extract of Pulsatilla koreana root was found to have potent anti-inflammatory and analgesic actions in intravenous administration in animals. Among chloroform, ethyl acetate, butanol and water fractions which were obtained through successive fractionation of the extract, only the water fraction was found to have the antiinflammatory and analgesic actions. The fraction did not affect normal body temperature at the effective doses in mice and showed low acute toxicity of which $LD_{50}$ was less than 500 mg/kg i.v. in mice. It is interesting that its anti-inflammatory action might be attributed in part to inhibition of cyclooxygenase-1 and -2.

• Journal of Plant Biotechnology
• /
• 제36권2호
• /
• pp.170-173
• /
• 2009

Leaf and petiole explants of Pulsatilla koreana NAKAI were cultured on MS medium supplemented with various concentrations of zeatin, kinetin or BAP combined with 0.05 mg/L IAA. After 6 weeks of culture, effects of cytokinin on adventitious shoot formation from explants were investigated. The highest frequency of shoot formation was obtained when petiole explants were cultured on medium with 0.5 mg/L zeatin and 0.05 mg/L IAA. Regenerated shoot were transferred on to root induction medium. The best root formation was observed at 1/2 MS medium with 1.5 mg/L NAA. Rooted plantlets were transplanted to a mixture of perlit and soil (1:3), where they were successfully acclimatized.

• Bulletin of the Korean Chemical Society
• /
• 제31권5호
• /
• pp.1159-1164
• /
• 2010

Several triterpenoid saponins from root of Pulsatilla koreana Nakai (Ranunculaceae) were studied and their biological activities were reported. It is difficult to analyze triterpenoid saponins using HPLC-UV due to the lack of chromophores. So, evaporative light scattering detection (ELSD) is used as a valuable alternative to UV detection. More recently, a charged aerosol detection (CAD) has been developed to improve the sensitivity and reproducibility of ELSD. In this study, we developed and validated a novel method of high performance liquid chromatography coupled with a charged aerosol detector for the simultaneous determination of four triterpenoid saponins: pulsatilloside E, pulsatilla saponin H, anemoside B4 and cussosaponin C. Analytes were separated by the Supelco Ascentis$^{(R)}$ Express C18 column (4.6 mm ${\times}$ 150 mm, 2.7 ${\mu}m$) with gradient elution of methanol and water at a flow rate of 0.8 mL/min at $30^{\circ}C$. We examined various factors that could affect the sensitivity of the detectors, including various concentrations of additives, the pH of the mobile phase, and the CAD range. Linear calibration curves were obtained within the concentration ranges of 2 - 200 ${\mu}g$/mL for pulsatilloside E, anemoside $B_4$ and cussosaponin C, and 5 - 500 ${\mu}g$/mL for pulsatilla saponin H with correlation coefficient ($R^2$) greater than 0.995. The limit of detection (LOD) and quantification (LOQ) were 0.04 - 0.2 and 2 - 5 ${\mu}g$/mL, respectively. The validity of the developed HPLC-CAD method was confirmed by satisfactory values of linearity, intra- and inter-day accuracy and precision. This method could be successfully applied to quality evaluation, quality control and monitoring of Pulsatilla koreana.

• 한국자원식물학회지
• /
• 제9권1호
• /
• pp.47-54
• /
• 1996

할미꽃의 뿌리에 함유되어 있는 제초활성물질의 확인과 동정을 위하여 이들을 추출 정제하고 활성성분 및 제초활성을 조사하여 다음과 같은 결과를 얻었다. 1. 각종 칼럼크로마토그래피법과 Prep. HPLC의 순차적인 적용에 의해 제초활성물질이 포함된 할미꽃 뿌리의 메타놀추출물을 정제하여 2개의 활성분획을 획득하였다. 2. 최종적으로 얻어진 활성분획들을 100ppm의 농도로 조제하여 처리한 결과, 식용피 유근의 생장 억제율은 대조구에 비해 각각 48% 및 60%로 나타났다. 3. GC-MS Spectrometry에 의해 분리된 활성분획 중의 성분들을 기존자료들과 비교하여 수종의 지방산 에스테르, 2종의 탄화수소류, squalene, evidonol 및 1종의 diazepin 관련 화합물이 동정되었다.

• 한국약용작물학회지
• /
• 제14권4호
• /
• pp.225-228
• /
• 2006

식물생장조절제의 종류와 농도를 모색하여 할미꽃의 발근 최적 조건을 조사하여 정상적인 재분화체를 획득하고자 하였다. 지금까지의 선행연구를 토대로 최적조건에서 shoot를 유도시킨 후 발근 유도 생장조절제에 바로 옮기면 shoot가 너무 작아 재분화체가 성장하지 못하고 고사되었다. 1/2 MS에서 키운 shoot를 3.0 mg/L NAA를 첨가한 배지에서 3일간 생장시킨 후, 1/3 MS로 옮겨 치상 한 경우, 약 10일 전후에 뿌리의 유도가 관찰되었다. 이렇게 뿌리가 유도된 재분화체는 염의 농도와 설탕의 농도를 동시에 순차적으로 낮추어 순화처리되었다.