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http://dx.doi.org/10.5010/JPB.2009.36.2.170

Effect of cytokinin on adventitious shoot formation and plant regeneration from explants of Pulsatilla koreana NAKAI  

Liam, Yu-Ji (Linyi normal university, Tongda road Linyi City,shandong Province)
Iin, Guan-Zhe (Linyi normal university, Tongda road Linyi City,shandong Province)
Kim, Won-Bae (Highland Agricultural Research Center of National Institute of Crop Science, RDA)
Yoo, Dong-Lim (Highland Agricultural Research Center of National Institute of Crop Science, RDA)
Zhao, Xiao-Mei (Linyi normal university, Tongda road Linyi City,shandong Province)
Publication Information
Journal of Plant Biotechnology / v.36, no.2, 2009 , pp. 170-173 More about this Journal
Abstract
Leaf and petiole explants of Pulsatilla koreana NAKAI were cultured on MS medium supplemented with various concentrations of zeatin, kinetin or BAP combined with 0.05 mg/L IAA. After 6 weeks of culture, effects of cytokinin on adventitious shoot formation from explants were investigated. The highest frequency of shoot formation was obtained when petiole explants were cultured on medium with 0.5 mg/L zeatin and 0.05 mg/L IAA. Regenerated shoot were transferred on to root induction medium. The best root formation was observed at 1/2 MS medium with 1.5 mg/L NAA. Rooted plantlets were transplanted to a mixture of perlit and soil (1:3), where they were successfully acclimatized.
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