Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of cytokinin on adventitious shoot formation and plant regeneration from explants of Pulsatilla koreana NAKAI

할미 꽃 (Pulsatilla koreana NAKAI) 식물 절편체로부터 부정아 유도에 미치는 cytokinin의 영향 및 식물체 재 분화

  • Liam, Yu-Ji (Linyi normal university, Tongda road Linyi City,shandong Province) ;
  • Iin, Guan-Zhe (Linyi normal university, Tongda road Linyi City,shandong Province) ;
  • Kim, Won-Bae (Highland Agricultural Research Center of National Institute of Crop Science, RDA) ;
  • Yoo, Dong-Lim (Highland Agricultural Research Center of National Institute of Crop Science, RDA) ;
  • Zhao, Xiao-Mei (Linyi normal university, Tongda road Linyi City,shandong Province)
  • 염옥희 (중국 산동성 임기사범대학교 생명과학부) ;
  • 임광철 (중국 산동성 임기사범대학교 생명과학부) ;
  • 김원배 (농촌진흥청 작물과학연구원 고령지 농업연구센터) ;
  • 유동림 (농촌진흥청 작물과학연구원 고령지 농업연구센터) ;
  • 조소매 (중국 산동성 임기사범대학교 생명과학부)
  • Published : 2009.06.30
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Abstract

Leaf and petiole explants of Pulsatilla koreana NAKAI were cultured on MS medium supplemented with various concentrations of zeatin, kinetin or BAP combined with 0.05 mg/L IAA. After 6 weeks of culture, effects of cytokinin on adventitious shoot formation from explants were investigated. The highest frequency of shoot formation was obtained when petiole explants were cultured on medium with 0.5 mg/L zeatin and 0.05 mg/L IAA. Regenerated shoot were transferred on to root induction medium. The best root formation was observed at 1/2 MS medium with 1.5 mg/L NAA. Rooted plantlets were transplanted to a mixture of perlit and soil (1:3), where they were successfully acclimatized.

Keywords

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