• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.472-476
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• 2004

Lateral roots formed on mungbean sprouts should lower their quality. The study was carried out to clarify the effects of aeration periods (AP; 1, 2, 3, and 4 hours) and temperature (AT; 20, 30, and $40^{\circ}C$) after 5 hour seed imbibition into 50 ppm benzyladenopurine (BA) solution on growth and morphological characters of mungbean (cv. Keumseongnogdu, Owoolnogdu and Zhong Lu 1) sprouts. On the 6th day, the mungbean sprouts were sorted by 4 categories on the base of hypocotyl lengths; > 7cm, 4 to 7cm, < 4cm, and non-germination to calculate their composition rates, number of lateral roots, lengths of hypocotyl and root, diameters at middle and upper parts of hypocotyl, fraction fresh and dry weights were measured. AP more affected growth of the cultivars than AT showing little effect on them. In the composition rate of the above 4 categories, cv. Zhong Lu 1 had the highest rate in longer than 7cm but nearly the same rate in AP treatments. Rates of longer than 4cm hypocotyls in cv. Keumseongnogdu, Owoolnogdu were increased with longer AP but their rates of shorter than 4cm showed reverse response to the former. Formation rate and number of lateral roots per sprout were decreased with longer AP, showing more severe decrement when delayed 3 to 4 hour AP. Upper part of hypocotyls and roots were more thickened and shortened in longer AP, respectively. Total fresh weights had no significant difference between AP treatments while hypocotyl fresh weights were increased with longer AP. It was concluded that in mungbean sprout culture aeration from BA treatment to the first watering permitted at least 4 hours.

• Journal of Bio-Environment Control
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• v.23 no.4
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• pp.288-292
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• 2014

This study was performed to investigate the possibility of water cutting by vegetative propagation method from July to August of Clematis. To find out proper cutting length and rooting growth regulator for water cuttings production, cutting lengths of C. 'Honora' made 1 node and 2 nodes length cutting, which were soaked in NAA $0.1mg{\cdot}L^{-1}$. Rooting rate of cuttings treated by NAA $0.1mg{\cdot}L^{-1}$ was 100% irrespective of root lengths, but that of 1 node and 2 nodes untreated by NAA $0.1mg{\cdot}L^{-1}$ were very low about 30% and 19% respectively. Number of roots and roots length were also highest in NAA $0.1mg{\cdot}L^{-1}$, effects of NAA $0.1mg{\cdot}L^{-1}$ treatment were greater at 1 node cutting. Based on the results, rooting rate for I node cutting of C. 'Pairu' according to NAA (0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$) concentrations was investigated. Days to rooting were 70-74 days (about 10 weeks), which was not significantly different among the NAA concentrations. Rooting rate increased by NAA concentration. it was about 90% usually and was improved with more than NAA $0.5mg{\cdot}L^{-1}$.

• Korean Journal of Microbiology
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• v.48 no.1
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• pp.16-21
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• 2012

Since many pesticides cause various health and environmental problems, alternative measures to replace them are needed, and the bacteria producing the antifungal substances can be one of them. In this study, several rhizobacteria were isolated and their antifungal activities against some important plant pathogenic fungi were examined. Pseudomonas otitidis TK1 and Paenibacillus peoriae RhAn32 inhibited the growth of Fusarium oxysporum f. sp. niveum and F. oxysporum f. sp. lycopersici by 49.8% and 45.6%, and 45.1% and 48.3%, respectively compared to those of the control. P. peoriae RhAn32 also decreased the growth of F. oxysporum f. sp. raphani by 37.5%. This growth inhibition might be due to the production of antifungal substances, such as siderophore, hydrogen cyanide and chitinase, which were produced by these rhizobacteria. P. otitidis TK1 also produced plant growth hormones indole acetic acid and indole butyric acid at $293.41{\mu}g/mg$ protein and $418.53{\mu}g/mg$ protein, respectively. When P. otitidis TK1 and B. cereus TK2 were inoculated together with F. oxysporum f. sp. lycopersici to the 4 weeks grown tomato seedlings and incubated additional 8 weeks, the stem lengths of tomato increased up to 45.7% and 55.3% and root lengths were raised to 64.9% and 60.8%, respectively than those of the control group. The wet weights increased by 118% and 182%, respectively compared to the control group.

• The Journal of the Korean dental association
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• v.47 no.12
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• pp.823-829
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• 2009

Purpose : The aim of this study was to evaluate the survival rate of sintered porous-surfaced implants placed in the edentulous posterior mandibles, in relation to implant length and diameter, crown-to-implant ratio, and types of prostheses, for a maximum of eight years of functioning. Material and Methods : The study group consisted of 43 partially edentulous patients who visited Catholic University Hospital of Daegu and one private dental clinic. A total of 122 sintered porous-surfaced implants n $Endopore^{(R)}$ (Inn ova Life Sciences, Toronto, Ontario, Canada) -- were placed in the edentulous posterior mandibles, Two diameter sizes (4.1 mm and 5.0 mm) and four lengths (5.0 mm, 7.0 mm, 9.0 mm, and 12.0 mm) were used. One hundred and three implants were splinted and 21 implants were nonsplinted. The survival rates of the implants in relation to length, diameter, crown-to-implant ratio, and types of prostheses were investigated. Statistical data were analyzed using SPSS Win.Ver 14.0 software with the Chi-square test. Results : The survival rate of the 4.1mm diameter implants was 100% and 91.2% for the 5.0mm diameter implants. The survival rates of the implants of differing diameters were found to be statistically different (p=0.005). The survival rates of both the 5.0mm and 7.0 mm length implants were 100%. The survival rate of the 9.0mm length implants was 97.9% and for the 12.0mm length implants was 95.1%. There was no statistical difference in survival rates for the differing lengths of implants. Of the 103 prostheses that were splinted, the survival rate was 98.0%. The survival rate of splinted prostheses was higher than that of the non-splinted prostheses, but was found to be not statistically different. There were no failed cases when the crown-to-implant ratio was under 1.0. When the crown-to-implant ratio was between 1.0 and 1.5, the failure rate of the implants was 6.7%. No failure was recorded with the ratio range of 1.5 to 2.0. Relative to the crown-to-implant ratio of 1.0, the failure rates were statistically different (p=0.048). Discussion and Conclusion : The cumulative survival rate of the porous-surfaced implants placed in the edentulous posterior mandibles was 97.5%. Short porous-surfaced implants showed satisfactory results after a maximum of nine years of functioning in the edentulous posterior mandibles.

• Korean Journal of Plant Resources
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• v.21 no.1
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• pp.23-27
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• 2008

Traditionally mungbean(Vigna radiata L.) sprouts has been eaten soon after washing. The study was carried out to measure the effect of washing before packaging on morphological characters and color of mungbean sprouts. The seeds of cv. Zhong Lu 1 were soaked in 50 ppm BA solution immediately before 4 hour aeration and then cultured for 6 days. The sprouts were washed immediately before packaged with PE envelops or not, and then stored 5 days at $8^{\circ}C$. Their morphological characters, fresh and dry weights, and colors were measured everyday. Compared to washed, nonwashed sprouts had more lateral roots although the two sprouts did nearly same in hypocotyl and root lengths, hypocotyl diameter, fresh and dry weights. Non-washed sprouts, moreover, showed higher brightness in hypocotyl and root and cutting resistance in hypocotyl although there were not significant differences in color a and b, meaning that the latter ones were more rapidly changed during their storage. In non-washed sprouts, number of lateral roots, hypocotyl length and diameter, total fresh and dry weights were nearly same up to 3 days and afterward were declined. Brightness and color b of hypocotyl were decreased with increased storage period although cutting resistance of hypocotyl was since 3 days after storage.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.6
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• pp.487-490
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• 2004

Methods for culturing bean sprouts could be divided into two main groups of overspraying and underwatering according to water supplying. The study was done to measure the effect of water supplying method on growth of mungbean sprouts and temperatures inside their culture boxes. The morphological characters, fresh and dry weights were measured on the 6th day after culturing, but temperatures of the boxes were recorded daily by dataloggers. Lateral roots was not formed in the under-watering method (UM) while partially done in the over-spraying method (OM). OM had longer hypocotyl but UM had longer root compared to the other. Two water supplying methods, however, had nearly similar total length adding hypocotyl and root lengths, diameters of middle and upper parts of hypocotyl. OM showed more total fresh weight than UM mainly resulting from increment of hypocotyl fresh weight. The fresh weight increment in OM was caused by relatively higher temperature of culture box compared to UM.

• Korean Journal of Plant Resources
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• v.20 no.2
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• pp.145-149
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• 2007

Shape of soybean sprouts affects their marketability. This study was carried out to understand the effects of pressure in both overspraying and underwatering cultivation methods on growth and morphological characteristics of soybean sprouts. Pressing treatments were done by continuous pressing by laying a plastic culture box over another with growing the sprouts, their alteration every day, or no pressing in overspraying method, and by continuous pressing, pressing after the 4th day cultivation with 30 kg steel case, or no pressing in underwatering method. The soybean (cv. Junjery) seeds were soaked in 2 ppm BA solution for 5 hours and then aerated for 3 hours immediately before 6 day culture. Lateral roots, hypocotyl and root lengths, hypocotyl diameters, fresh and dry weights were measured. Lateral roots per sprout were decreased with stronger pressing regardless of watering methods. In overspraying method, continuous pressing treatment resulted in shortest hypocotyl, but no and ones did the longest root. In underwatering method, however, no pressing one showed the longest hypocotyl but continuous pressing did the shortest hypocotyl. Regardless of watering methods, middle part of hypocotyl was more thickened with stronger pressing although hook diameters of the 3 treatments were not affected. In overspraying method. total fresh weight per sprout was the least in no pressing but the greatest in continuous pressing. In underwatering method, however, there was no significant difference between the treatments.

• Korean Journal of Plant Resources
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• v.19 no.2
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• pp.344-347
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• 2006

Watering methods for soybean sprouts could be mainly divided into two groups of overspraying and underwatering. The study was carried out to determine the effect of water supplying method on growth, morphological characteristics, colour and cutting resistance of soybean (cv. Junjery) sprouts and culture temperatures. The morphological characters, fresh and dry weights were measured on the 6th day after their culture, but daily mean temperatures inside the plastic culture boxes were measured by data-loggers. Lateral roots were more formed in the underwatering method (UM) than in the overspraying method (OM). Although their total lengths of both methods were nearly same, OM had longer hypocotyl but UM did longer root than the other. Middle and upper parts of hypocotyl were more thickened in UM than in OM. UM showed more hypocotyl fresh and dry weights than OM. There was, however, no significant difference between the two methods in cotyledon, root, total fresh and dry weights although the culture temperature was higher in OM than in UM.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.24-24
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• 2017

Heavy metals at toxic levels have the capability to interact with several vital cellular biomolecules such as nuclear proteins and DNA, leading to oxidative stress in plants. The present study was performed to explore the metal tolerance mechanism in Sorghum seedling. Morpho-physiological and metal ions uptake changes were observed prominently in the seedlings when the plants were subjected to different concentrations of $CuSO_4$ and $CdCl_2$. The observed morphological changes revealed that the plants treated with Cu and Cd displayed dramatically altered shoot lengths, fresh weights, and relative water content. In addition, the concentration of Cu and Cd was markedly increased by treatment with Cu and Cd, and the amount of interacting ions taken up by the shoots and roots was significantly and directly correlated with the applied level of Cu and Cd. Using the 2-DE method, a total of 24 and 21 differentially expressed protein spots from sorghum leaves and roots respectively, 33 protein spots from sorghum leaves under Cd stress were analyzed using MALDI-TOF/TOF MS. However, the over-expression of GAPDH plays a significant role in assisting Sorghum bicolor to attenuate the adverse effects of oxidative stress caused by Cu, and the proteins involved in resistance to stress helped the sorghum plants to tolerate high levels of Cu. Significant changes were absorbed in the levels of proteins known to be involved in carbohydrate metabolism, transcriptional regulation, translation and stress responses. In addition, the up-regulation of glutathione S-transferase and cytochrome P450 may play a significant role in Cd-related toxicity and stress responses. The results obtained from the present study may provide insights into the tolerance mechanism of seedling leaves and roots in Sorghum under heavy metal stress.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.1
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• pp.28-33
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• 2008

Traditionally mungbean (Vigna radiata L.) sprouts has been stored after dehydration to decrease their decay. The study was done to determine the effect of watering for final 12 hours of harvest day on morphological characters and color of mungbean sprouts. The seeds of cv. Zhong Lu 1 were soaked in 50 ppm BA solution immediately before 4 hour aeration and then cultured for 6 days, when water-supplied for final 12 hours or not. After packaged with PE envelops, the sprouts stored 5 days at $8^{\circ}C$, and their morphological characters, fresh and dry weights, and colors were measured everyday. Compared to non-water supplied, water supplied sprouts had thicker hypocotyl diameter in middle part, and higher total fresh weight due to increment of cotyledon and hypocotyl ones although the two sprouts did nearly same in other characters. Only nonwater supplied sprouts showed higher color b value in hypocotyl than water supplied ones although there were not significant differences between them in other color-related characters, meaning that the latter ones were more rapidly decayed during their storage. In non-water supplied sprouts, number of lateral roots, hypocotyl and root lengths, total fresh weight, brightness of hypocotyl and root was decreased since 3 days after storage but cutting resistance of hypocotyl was done since 5 days.