• Korean Journal of Environmental Biology
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• v.20 no.4
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• pp.355-359
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• 2002

This study was carried out to investigate the combined effect of cold storage with irradiation on rooting of chrysanthemum cuttings. Each cutting was six cm in length with three real leaves. A set of Groups of the cuttings was irradiated with 0 to 70Gy of gamma-ray after four weeks of cold storage, while another groups were irradiated with the same doses and then stored at $4^\circ{C}$ for four weeks. Root and shoot growth were observed at 15 and 20 days after planting of the cuttings in a Plug tray. Accelerated root formation was observed in 30 Gy irradiated group, and root growth was also enhanced in 20 and 30 Gy irradiated groups 20 days after planting. In case of irradiation combined with pre-treatment of cold storage, survival rate of cuttings was the highest (80%) in 30 Gy irradiated group. The groups treated with irradiation first before cold storage were apparently better in root formation, among which 50 Gy irradiated group showed the highest rate of root growth.

• Journal of Bio-Environment Control
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• v.31 no.1
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• pp.35-42
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• 2022

To investigate the effects of ozonated water concentration and soaking time on adventitious root formation of willow, we studied the efficiency of root cuttings in the revegetation technology of biological engineering of willows. The ozonated water concentrations were used for 5 minutes and 2 hours at 1, 5, 10, 15, 20 ppm by soaking method and then the shoot characteristics were observed. The number and length of adventitious roots were determined. The results indicated that 20 ppm of ozonated water for 2 hours and 1 ppm of ozonated water for 5 minutes resulted in leaves turned wither away and no adventitious root production. Considering the appearance, number and length of the adventitious root, soaking willow cuttings into the ozonated water with dissolved ozone concentration, 5 ppm for 2 hours and 10-15 ppm for 5 minutes were suitable for generating adventitious roots.

• Journal of the Korean Institute of Landscape Architecture
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• v.34 no.3 s.116
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• pp.79-90
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• 2006

Tamarix chinenis blooms twice a you and its flowers, branches and leaves make the adjustment of tree shape. Propagation methods and growth characteristics of T. chinensis were studied in order to ascertain its potential use as one of vegetation resources for coast forestation and landscaping. The study results indicated that 1 or 2 you old hard wood cuttings showed higher rooting ratio than greenwood or semi hard wood cuttings. One to one mixture between vermiculite and pearlite appeared to be the best for bed soil, and sea sand and silt(loess) mixture was the next. Sea sand and granitic soil followed after. In terms of seasonal differences, spring cuttings showed the best rooting ratio, root number, and root length. Fall cuttings followed after spring cutting, and summer cuttings showed worst results regarding rooting ratio, root number, and root length. The best rooting promotion effects of growth regulators were observed with sea sand bed soils. There was no significant difference among growth regulators in terms of rooting and shoot growth. Low concentration below 100 ppm of growth regulators was enough for rooting promotion effect. In general, the number and mean length of roots and shoots were showed the excellent records in the sites with high rooting ratio. The study result strongly showed that T. chinensis can be considered as a suitable tree for coast forestation and landscaping because of its easy cutting propagation and rapid growth on saline lands.

• Journal of Korean Society of Forest Science
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• v.52 no.1
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• pp.1-30
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• 1981

The origin and development of adventitious roots was studied using hypocotyl and epicotyl cuttings of 34 species, 24 genus of woody plants. These cuttings obtained from young seedlings cultured in vials containing distilled water only. The several characteristics of cuttings materials studied are shown in Table 1. The results are summerized as follows: 1. The circumference shapes of cross-sections of hypocotyl and epicotyl cuttings can be divided into six categories, namely, round, irregular round, ellipse, irregular ellipse, square, and triangle. Species differences within a genus did not show any difference of hypocotyl and epicotyl cross-sections shape, however, a noticeable variation among genus or higher taxa. 2. The arrangements of vascular bundles in the cross-sections of hypocotyls or epicotyls were almost all collateral types and generally showed generic characteristics differing one to the other. However, there were some variations between species within the genus. Six models of vascular bundle arrangement were proposed for all the above speices. 3. The rooting portions of hypocotyl and epicotyl cuttings in this experimental materials can be grouped as follows: (1) Interfascicular parenchyma; (Thuja orientalis. T. orientalis for. sieboldii, Acer microsieboldianum, A. palmatum, A. saccharinum, Cercis chinensis, Lespedeza bicolor, Magnolia obovata, M. sieboldii, Mallotus japonicus, Staphylea bumalda) (2) Cambial and phloem parenchyma: (Chamaecyparis obtusa, C. pisifera, Albizzia julibrissin, Buxus microphylla var. Koreana, Cereis chinensis, Euonymus japonica, Firmiana platanifolia, Lagerstroemia indica, Ligustrum salicinum, L. obtusifolium, Magnolia kobus, M. obovata, Mallotus japonicus, Morus alba, Poncirus trifoliata, Quercus myrsinaefolia, Rosa polyantha, Styrax japonica, Styrax obassia) (3) Primary ray tissues; (Euonymus japonica, Styrax japonica) (4) Leaf traces; (Quercus acutissima, Q. aliena) (5) Cortex parenchyma; (Ailanthus altissima) (6) Callus tissues; (Castanea crenata, Quercus aliena, Q. myrsinaefolia, Q. serrata) 4. As a general tendency throughout the species studied, in hypocotyl cuttings, the adventitious root primordia were originated from the interfascicular parenchyma tissue, however, leaf traces and callus tissues were contributed to the root primordia formation in epicotyl cuttings. The hypocotyl cuttings of Ailanthus altissima exhibited a special performance in the root primordia formation, this means that cortex parenchyma was participated to the origin tissue. And in Firmiana platanifolia, differening from the other most species, the root primordia were formed at the phloem parenchyma adjacent outwardly to xylem tissue of vascular bundle system as shown photo. 48. 5. All the easy-to, or difficult-to root species developed adventitious roots in vials filled with distilled water. In the difficult-to-root species, however, root formations seemed to be delayed because they almost all had selerenchyma or phloem fiber which gave some mechanical hindrance to protrusion of root primordia. On the other hand, in the easy-to-root species they seemed to form them more easily because they did not have the said tissues. The rooting portions between easy-to-root and difficult-to-root species have not clearly been distinguished, and they have multitudinous variations. 6. The species structured with the more vascular bundles in number compared with the less vascular bundles exhibited delayed rooting. In the cuttings preparation, the proximal end of cuttings was closer to root-to-stem transition region, the adventitious root formation showed easier. 7. A different case occured however with the mature stem cuttings, in both the needle-leaved and the broad-leaved species. In the hypocotyl cuttings, parenchymatous tissues sited near the vascular bundles become the most frequent root forming portions in general and relevant distinctions between both species were hardly recognizable. 8. In the epicotyl cuttings, root primordia originated mainly in leaf traces in connection with cambial and phloems or callus tissues itself. In the hypocotyl cuttings, interfascicular parenchyma was the most frequent portion of the root primordia formation. The portions of root primordia had more connection with vascular cambium system, as the tissues were continuing to be developed.

• Journal of Environmental Science International
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• v.12 no.11
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• pp.1189-1196
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• 2003

The effect of boron and aluminum on the development of adventitious roots was studied in sunflower cuttings. Three-day-old seedlings were de-rooted and grown in nutrient solutions with or without boron and supplemented with different concentrations (from 50 to 700 ${\mu}$M) of aluminum. The number and length of the adventitious roots and proline content in adventitious roots in response to insufficient boron and aluminum stress were determined periodically. The micronutrient boron caused the development of numerous roots in the lower parts of the hypocotyl. A dose-response of boron-induced rooting yielded an optimum concentration of 0.1 mM boron. In the absence of boron, in the majority of the adventitious roots, a significant inhibition was observed with or without aluminum, indicating that the most apparent symptom of boron deficiency is the cessation of root growth. Increasing concentrations of aluminum caused progressive inhibition of growth and rooting of the hypocotyls, and a parallel increase in proline levels of adventitious roots. Supplemental boron ameliorated the inhibitory effect of aluminum, suggesting that aluminum could inhibit root growth by inducing boron deficiency. Ascorbate added to medium in the absence of boron improved root growth and induced a significant decrease in proline levels. These findings suggest that adventitious root growth inhibition resulting from either boron deficiency or aluminum toxicity may be a result of impaired ascorbate metabolism.

• Journal of Korean Society of Forest Science
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• v.1 no.1
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• pp.12-21
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• 1962

Outdoor cuttings studies on two coniferous species, pitch pine and needle fir, have been made at Suwon, Korea extending from March to early May, 1956. The purpose of these studies was to find the effects of ages of parent trees, topophysis, dilute hormone solution treatments and the time at which the cuttings were collected on the rooting response of the cuttings. On September 2, rooting results were examined. Pitch Pine (Pinus rigida) The total of 1335 cuttings were collected from 3- and 10-year old trees. The cuttings treated were planted on March 13 and April 20. 1. In the cuttings collected from 3-year-old seedlings, the best result (34 percent rooted) was obtained by treating ends of cuttings with a mixture of 50 ppm IBA and 50 ppm NAA. 2. No rooting was observed in lammas shoots. 3. The cuttings from 3-year-old seedlings gave better rooting than those from 10-year-old trees. 4. The root origins were developed from the lower part of the cutting and the cut surface. Needle fir (Abies holophylla) One hundred twenty-five cuttings were collected from a 30-year-old tree. The cuttings treated with any one of four mixed hormone solutions were planted on March 23. 1. Fifteen cuttings out of 75 cuttings from the lower half of the crown were rooted. The cuttings obtained from the upper half of the crown did not root at all. 2. The cuttings treated by the hormone solution made up of 20 ppm IAA, 20 ppm IBA, 20 ppm NAA, 20 ppm thiamine and 5 percent sucrose gave the best rooting (22 percent). 3. All roots originated from callus tissues and irregularly arranged parenchymatous tissues near the cut base.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.6
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• pp.25-28
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• 2003

This study was conducted to development of rapid propagation method by leaf cuttings in Hibiscus hamabo native to southern seaside of Korea, and special object of this study was to determine the effects of IAA, IBA, and NAA on rooting in leaf cuttings of H. hamabo. Rooting was promoted by dipping treated with IAA and IBA. And rooting percentage was greatest at 1,000 and 2,000$mg{\cdot}L^{-1}$ IBA. At higher concentrations of IAA and IBA, more adventitious roots were developed. Also, IAA at high concentrations (above 2,000$mg{\cdot}L^{-1}$) and IBA ranged from 500~2,000$mg{\cdot}L^{-1}$ promoted on root number and rootingratio. However, root formation in Hibiscus leaf cuttings inhibited by NAA application.

• Journal of Sericultural and Entomological Science
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• v.33 no.2
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• pp.68-71
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• 1991

Mulberry cuttings from shoots of Shinkwangppong(Morus bombycis koidz.) had been callused in vermiculite separately at 15, 20, 25 and 30$^{\circ}C$ for 15 days before transplanting them in greenhouse to make clear the effect of temperature on root formation and growth is as follow. The buds of cuttings started sprouting in 4 and 6 days of callusing at 30 and 25$^{\circ}C$, respectively, reaching 100% budding in 10 and 15 days of callusing. Budding was delayed, however, at low temperature, showing 86% and 92% at 15 and 20$^{\circ}C$, respectively, in 15 days. Rooting from the cuttings was also accelerated at high temperature, showing 97-100% rooting at 25$^{\circ}C$ and 30$^{\circ}C$, in 15 days of callusing but no more than 93% at low temperature even in 35 days. Although high temperature increased root number and length after 15 days in callusing, no differences showed in the number and the weight at more than 20$^{\circ}C$ in 35 days of cuttings.

• Korean Journal of Environment and Ecology
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• v.7 no.1
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• pp.6-9
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• 1993

To examine aluminum toxicity on woody plants, Forsythia koreans and Platanus occidentalis cuttings were grown in the pot(48$\times$33$\times$9cm) filled with sand, and treated aluminum solution and ground water (pH 6.75) 3times per week from April 28, 1993 to June 16. Aluminum solution were prepared 1.0, 2.5 and 5.0mM aluminum potassium sulfate, dilulted with ground water. Growth-related characters (Shoot growth, leaf number and leaf chlorophyll content ) and root growth were measured and compared among the treatments. In all growth-related characters(Shoot growth, leaf number and leaf chlorophyll content), differences among the treatments were highly significant. In root growth, differences among the treatments were highly significant for Forsythia koreana cuttings, but not for Platanus occidentalis cuttings.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.3
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• pp.56-68
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• 2003

The main purpose of this study was to verify the shore margin protection effect of the root system of Salix gracilistyla Miq. developed from direct sticking cuttings on wetland, through the measurement of root growth and comparison of soil slaking rate depending on the planting method applied to shore-marginal slope. Comparison of growth rate and soil dispersion rate was made between five planted slope and one naked slope. The planting methods applied to the planted slope were (a) horizontally layed burying of stick(45cm) bundle (b) horizontally layed covering the slope with sticks (c) horizontally fencing with normal cuttings(20cm) (d) elected sticking of normal cutting at equal distances (e) random scattering short cuttings(3-4cm). As results, the most effective planting method was horizontally layed burying, and in order to increase its efficiency scattering the live stem chips in 2-3cm on the slope is recommended. The growth of root was negatively regressive to the distance from water floor.