• Title/Summary/Keyword: root culture

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The Effects of Auxin and Casein Hydrolysate on the Growth of Ginseng Hairy Root (인삼모상근의 생장에 미치는 Auxin과 Casein Hydrolysate의 영향)

  • 오승용;박효진;민병훈;양계진;양덕춘
    • Journal of Ginseng Research
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    • v.24 no.3
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    • pp.123-127
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    • 2000
  • In this study, the effects of auxin and casein hydrolysate (CH) on the growth of ginseng hairy root was elucidated. Ginseng hairy root was cultured under light and dark conditions in MS solid and liquid medium with various concentrations of auxin and CH for fifty days. After harvesting the cultures, the fresh and dry weight of cultures were examined, respectively. In the MS solid culture, 1 mg/L of IBA was most effective on the growth of ginseng hairy root under the dark condition, whereas IAA and CH did not affect on the growth of ginseng hairy root. In the MS liquid culture, the growth was maintained regularly by the treatments of IAA and NAA. IBA and CH restrained the growth of ginseng hairy root.

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Growth, yield and mineral element responses to temperature in Codonopsis lanceolata

  • Kwon, Soo Jeong;Han, Eun Ji;Moon, Young Ja;Cho, Gab Yeon;Boo3, Hee Ock;Lee, Moon Soon;Woo, Sun Hee;Kim, Hag Hyun
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.274-274
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    • 2017
  • Temperature has an adverse effect for potential growth and development of Codonopsis lanceolata. The present study examined the responses of growth, yield and mineral element contents under temperature variables in C. lanceolata. Plant height exhibited the best result as being 2 m or higher the open field culture, while the growth is the most restricted in the $30^{\circ}C$. The open field culture also had the largest number of leaves in the early stage of growth. The number of leaves and the height tended to be increased with the temperature of $20^{\circ}C$ and $25^{\circ}C$. The number of branches showed no significance as 32 branches and 26 branches in open field culture and $20^{\circ}C$, respectively. The smallest number of branches was observed in the $30^{\circ}C$ with 15 branches. The leaf color recorded the lowest value in the open field culture unlike the growth above the ground. But all other temperature had no significance with the leaf color value between 50.0 and 52.6. The elevated temperature induced the thinner and shorter diameter and length of root. However, the root diameter was not significantly changed with the change of 1.8 cm and 2.1 cm at open field culture, with $20^{\circ}C$ and $25^{\circ}C$, respectively. The fresh weights of the primary root and lateral roots were 6.3 g and 3.1 g, respectively, at the high temperature. The result showed the thickening of the lowest part under the ground as restricted. The fresh weights of the main root and lateral root were the highest on the open field culture, 20.6 g and 12.2 g, respectively. The contents of cellulose were the highest as 4.41% in the $20^{\circ}C$, followed by the open field culture, $25^{\circ}C$ and $30^{\circ}C$. The higher temperature reduced the contents of cellulose. Moreover, P showed higher ratio in the high temperature while Ca and Mg exhibited the higher ratio towards the low temperature. On the contrary, minor quantity (less than 1%) of mineral nutrients was observed towards all temperature variables.

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Production of Shikonin by A Hairy Root Culture of Lithospermum erythrorhizon

  • Seo, Weon-Taek;Park, Young-Hoon;Choe, Tae-Boo
    • Journal of Microbiology and Biotechnology
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    • v.2 no.1
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    • pp.41-45
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    • 1992
  • Shikonin production was examined in a bubble column bioreactor system with the hairy roots of Lithosphermum erythrorhizon. The volumetric productivity was higher than those obtained from other reactor configurations with free or immobilized cells of the same cell line. The productivities of the bubble column reactor, with and without a product absorption trap, were 7.4 and 4.5 mg of shikonin/l/d, respectively. This indicated the importance of the product removal in the design and operation of the shikonin production system with hairy root culture.

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Hairy Root Culture of Daucus carota for Anthocyanin Production in a Fluidized-bed Bioreactor (유동층 생물반응기에서 anthocyanin 생산을 위한 당근의 모상근 배양)

  • Kim, C.H.;Lee, S.W.;Chung, I.S.
    • Applied Biological Chemistry
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    • v.37 no.4
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    • pp.237-242
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    • 1994
  • Hairy root culture of Daucus carota was investigated for anthocyanin production in a fluidized-bed bioreactor. The growth of hairy roots in this bioreactor increased 2.5 fold while anthocyanin production was lower. However, the anthocyanin production of hairy roots in a fluidized-bed bioreactor was enhanced 2.3 fold in response to the treatment of the fungal elicitor.

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Enhanced Neurite Outgrowth of Dorsal Root Ganglion Sensory Neurons after Sibjeondaebo-tang Treatment

  • Kwon, Ku-Birm;NamGung, Uk
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.24 no.4
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    • pp.681-687
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    • 2010
  • Sibjeondaebo-tang (SJDBT) is an oriental medicinal prescription for the treatments of diverse symptoms including neurological disorders. In order to investigate its potential role for neural regulation following nerve injury, neurite outgrowth of dorsal root ganglion (DRG) neurons in culture was investigated. In DRG neurons which were preconditioned by sciatic nerve injury, neurite outgrowth was enhanced by SJDBT treatment. When preconditioned DRG neurons were co-cultured with astrocytes prepared from injured spinal cord tissue, neurite outgrowth was similarly facilitated by SJDBT. Astrocytes in co-culture showed more intense signals of vimentin protein by SJDBT compared to saline control. Sukjihwang (SJH), a conventional herbal component of SJDBT prescription, did not induce any significant changes in neurite extension of DRG neurons compared to control cells. These data suggest that SJDBT may be the therapeutic agent for nervous system disorders related to nerve damage.

Regeneration from Storage Root Disk Culture of Purple Sweet Potato

  • Park, Hyejeong;Park, Hyeonyong
    • Korean Journal of Plant Resources
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    • v.28 no.3
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    • pp.363-369
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    • 2015
  • Sweet potato has low regeneration capacity, which is a serious obstacle for the fruitful production of transgenic plants. Simple and rapid regeneration method from storage root explants of purple sweet potato (Ipomoea batatas L.) was investigated. The embryogenic callus was observed from 4 cultivars and its highest rate was induced at 1 μM 2,4-D after 5 weeks of culture. Result revealed that a low concentration of 2,4-D and low light intensity was important factors for embryogenic callus formation. After subculture on medium with 5 μM ABA for 4 days, subsequently, occurred the regeneration of shoots within 4 weeks when these embryogenic callus was transferred onto the MS hormone free medium. Regenerated shoots were developed into platelets, and grown normal plants in the greenhouse. We developed a simple and quickly protocol to regenerate plantlets in storage root explants of purple sweet potato. This regeneration system will facilitate tissue culture and gene transfer research of purple sweet potato.

EFFECT OF CULTURE SUPERNATANT OF BACTERIA ISOLATED FROM INFECTED ROOT CANALS ON CELL LINES (감염 근관에서 분리한 세균 배양액이 배양된 세포에 미치는 영향)

  • Im, Mi-Kyung
    • Restorative Dentistry and Endodontics
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    • v.22 no.2
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    • pp.761-768
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    • 1997
  • Microorganisms and their by-products are considered to be the major causes of pulpal and periapical pathosis. The role of microorganisms in endodotic infection has been studied regarding the prevalence of particular organisms found in root canal and periapical lesions. The aim of this study was to investigate the effect of culture supernatants of several oral microorganims isolated from infected root canals on the viability of cultured cell lines using colorimetirc assay. S. simulans, S. sciuri, E. faecium, S. intermedius, S. mitis, S. sanguis and S. uberis were incubated in Todd-Hewitt broth for 16 hours. 20 and 100ul of filtered bacterial cell culture supernatants were added to MK and Hep-2 cells. Cell viability was measured using MIT colorimetric assay. 20ul and 100u1 of S. sanguis supernatant showed significant cytotoxicity compared to control on MK cells. 100ul of S. sanguis supernatant significantly depressed viability of HEp-2 cells. E. faecium and S. intermedius did not affect the viability of MK and HEp-2 cells.

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Effects of Nutrient Concentrations for Each Growing Stages on Beet(Beta vulgaris L. cv. Detroit) Growth (생육단계별 배양액농도가 비트(Beta vulgaris L. cv. Detroit)의 생육에 미치는 영향)

  • 박권우;강호민;박용건
    • Journal of Bio-Environment Control
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    • v.5 no.2
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    • pp.138-144
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    • 1996
  • This experiment was conducted to decide appropriate nutrient concentration for beet soilless culture by different nutrient concentration treatment between before and after root enlargement. Yamazaki's solution for head lettuce was used 0.5, 1, 2, 4 strength for before root enlargement and 0.5, 1, 2, 4 strength was treated after root enlargement. Root weight was good in 0.5, 1, 2 strength treatment before, then change treatment to 4 strength after root enlargement. But 4 strength treatment after root enlargement increase pithiness and root cracking. Vitamin C contents was low in 0.5 and 4 strength treatments after root enlargement. Betaxanthin and betacyanin were involved high concentration in lower nutrient concentration after root enlargement despite the treatments of before root enlargement. Therefore 1, 2 strength before root enlargement and 2 strength after root enlargement treatments is recommendable for nutrient culture of beet.

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Effect of Chlorella Culture Solution Using Anaerobic digestate on Seed Germination in Perennial Ryegrass (혐기소화액을 배지로 이용한 클로렐라 배양액 처리가 페레니얼라이그라스 종자 발아에 미치는 영향)

  • Byeon, Ji-Eun;Lee, Jin Woong;Choi, Min Soo;Ryoo, Jong-Won
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.38 no.1
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    • pp.7-15
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    • 2018
  • This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

Ginseng Tissue Culture (인삼(人蔘)의 조직배양(組織培養))

  • Lee, Jae-Du
    • Korean Journal of Pharmacognosy
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    • v.3 no.2
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    • pp.65-72
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    • 1972
  • Tissues of $Panax\;Schinseng\;N_{EES}\;root$ were cultured on the synthetic agar media to investigate the nutrient efficiency on the callus induction and organ formation. The differentiation pattern of the callus mass and the structure of the induced organ (root) were observed internally. On White's medium, callus formation needed the supplement of 2,4-D (5mg/l) and kinetin (1.0mg/l), and on MS medium the root induction NAA (0.2mg/l) and kinetin (0.1mg/l). In order to investigate the effect of inorganic components on callus formation, the inorganic part of White' medium was substituted with those of Heller, Murashige Skoog, and Earle. As the result culture Earle's was most effective. On the other hand, the roots were induced from the meristem in the deep region of callus mass. Since this meristem is similar to the pericambium of tap root, they are the same on the pattern of morphogenesis.

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