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http://dx.doi.org/10.7732/kjpr.2015.28.3.363

Regeneration from Storage Root Disk Culture of Purple Sweet Potato  

Park, Hyejeong (Department of Biology, Chosun University)
Park, Hyeonyong (Department of Biology, Chosun University)
Publication Information
Korean Journal of Plant Resources / v.28, no.3, 2015 , pp. 363-369 More about this Journal
Abstract
Sweet potato has low regeneration capacity, which is a serious obstacle for the fruitful production of transgenic plants. Simple and rapid regeneration method from storage root explants of purple sweet potato (Ipomoea batatas L.) was investigated. The embryogenic callus was observed from 4 cultivars and its highest rate was induced at 1 μM 2,4-D after 5 weeks of culture. Result revealed that a low concentration of 2,4-D and low light intensity was important factors for embryogenic callus formation. After subculture on medium with 5 μM ABA for 4 days, subsequently, occurred the regeneration of shoots within 4 weeks when these embryogenic callus was transferred onto the MS hormone free medium. Regenerated shoots were developed into platelets, and grown normal plants in the greenhouse. We developed a simple and quickly protocol to regenerate plantlets in storage root explants of purple sweet potato. This regeneration system will facilitate tissue culture and gene transfer research of purple sweet potato.
Keywords
Ipomoea batatas; Embryogenic callus formation; Somatic embryogenesis; Plant Regeneration;
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