Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
권호 표지
DOI QR코드

DOI QR Code

Effect of Chlorella Culture Solution Using Anaerobic digestate on Seed Germination in Perennial Ryegrass

혐기소화액을 배지로 이용한 클로렐라 배양액 처리가 페레니얼라이그라스 종자 발아에 미치는 영향

  • Received : 2017.10.15
  • Accepted : 2018.02.27
  • Published : 2018.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

This experiments were conducted to evaluate the influence of Chlorella culture solution using anaerobic digestate as medium on seed germination of perennial ryegrass seeds. Four treatments were compared: control with distilled water, anaerobic digestate, Chlorella culture solution and Chlorella culture filtrate. The germination percentage of perennial ryegrass seeds was highest in the Chlorella culture solution treatment. Days required for 50, 70% seed germination were faster at 1.7 day in Chlorella culture solution compared to control. Root length of perennial ryegrass seeds was longer by 1~2cm in the Chlorella culture solution compared with control. The relative root length was by 40% longer in the Chlorella culture solution treatment compared to control. The germination index (GI) of perennial ryegrass seeds was higher by 180~202% in the Chlorella culture solution treatment compared to control. The decay rate was low as 50.0% in Chlorella culture solution, but decay rate of perennial ryegrass seeds showed 86.7~83.3% in control plot and in anaerobic digestate, respectively. Chlorella culture solution have shown stimulatory effects in germination and development of root. Overall, Chlorella culture solution could be useful to apply for promotion of germination and root elongation of seeds.

본 연구는 혐기소화발효액을 배지로 이용하여 클로렐라를 배양한 배양액이 페레니얼라이그라스 종자 발아에 미치는 영향을 구명하기 위하여 혐기소화발효액, 클로렐라 배양액, 클로렐라 배양여액, 혐기소화발효액, 무처리구(물)을 처리하여 종자 발아시험을 수행하였다. 페레니얼 라이그라스 종자의 최종 발아율은 무처리구의 70.2%와 비교하여 클로렐라 배양액 처리구가 91.9%로 21.2% 높았다. 또한 상대발아율도 클로렐라 배양액 처리구가 클로렐라 배양액을 처리하지 않은 무처리구(물) 보다 25% 높았다. 페레니얼 라이그라스 종자의 50% 발아에 소요되는 일수(T50)는 클로렐라 배양액 3.3~3.5일로 무처리구의 4.7~5.1일보다 빨랐다. 페레니얼 라이그라스 종자의 뿌리길이는 클로렐라 배양액 처리구가 무처리(물)보다 1~2cm이상 길었다. 상대적 뿌리신장율은 클로렐라 배양액 처리구의 무처리구보다 40% 높았다. 페레니얼 라이그라스 종자의 발아지수(GI)는 클로렐라 배양액 처리구가 182로 무처리구(물)의 100 보다 높았다. 종자의 부패율은 치상 후 11일째에 무처리구와 혐기소화발효액 처리구가 각각 83.3, 86.7%으로 높았으나 클로렐라 배양액 처리구는 50.0%로, 클로렐라 배양여액 처리구가 53.3%로 부패율이 낮았다. 클로렐라 배양액, 클로렐라 배양여액 처리가 종자의 부패방지 효과를 나타내었다.

Keywords

References

  1. Andersen, R.A. 2005. Algal culturing techniques. Elsevier Academic Press, San Diego, C.A., USA. pp. 239-287.
  2. Dobrznski, A., Anyszka, Z. and Elkner, K. 2008. Reakcja marchwi na ekstrakty pochodzenia naturalnego z alg z rodzaju Sargassum. Algaminoplant i z Leonardytu. HumiPlant. Journal of Research and Applications in Agricultural Engineering. 53:53-58.
  3. Hong, D.D., Hien, H.M. and Son, P.N. 2007. Seaweeds from Vietnam used for functional food, medicine and biofertilizer. Journal of Applied Phycology. 19:817-826. https://doi.org/10.1007/s10811-007-9228-x
  4. Khan, W., Rayirath, U.P., Subramanian, S., Jithesh, M.N., Rayorath, P., Hodges D., Critchley, A.T., Craigie, J.S., Norrie, J. and Prithiviraj B. 2009. Seaweed extracts as biostimulants of plant growth and development. Journal of Plant Growth Regulation. 28:386-399. https://doi.org/10.1007/s00344-009-9103-x
  5. Kim, S.E., Kang, C.K. and Lee, J.M. 2001. Effect of SMP treatment and storage after priming on germination and seedling growth in watermelon. Journal of The Korean Society For Horticultural Science. 42:43-47.
  6. Kim, S.S., Park, M.K. Oh, N.S., Kim, D.C., Han, M.S. and In, M.J. 2003. Studies on quality characteristics and shelf-life of Chlorella soybean curd (Tofu). Journal of the Korean Society for Applied Biological Chemistry. 46:12-15.
  7. Kim, M.J., Shim, C.K., Kim, Y.K., Park, J.H., Hong, S.J., Ji, H.J. and Yoon, J.C. 2014. Effect of Chlorella vulgaris CHK0008 Fertilization on Enhancement of Storage and Freshness in Organic Strawberry and Leaf Vegetables. Korean Journal of Horticultural Science and Technology. 32:872-878. https://doi.org/10.7235/hort.2014.14107
  8. Kong, H.G., Chun, O.J., Choi, K.H., Lee, K.Y., Woo, J., Baek, H.J., Kim., Murugaiyan, S., Moon, B.J. and Lee, S.W. 2010. Formulation of Bacillus amyloliquefaciens A-2 and its efficacy to control tomato leaf mold caused by Fulvia fulva. Research in Plant Disease. 16:27-34. https://doi.org/10.5423/RPD.2010.16.1.027
  9. Kulik, M.M. 1995. The potential for using Cyanobacteria (bluegreen algae) and algae in the biological control of plant pathogenic bacteria and fungi. European Journal of Plant Pathology. 101:585-599. https://doi.org/10.1007/BF01874863
  10. Lee, S.B. 2017. Case studies on practical Application of Chlorella farming technique. Doctor's degree thesis of Kongju National University, Chungnam, Korea.
  11. McKeon, T.A. and Stumpf, P.K. 1982. Purification and characteri- zation of the stearoyl-acyl carrier protein desaturase and the acyl- acyl carrier protein thioesterase from maturing seeds of safflower. Journal of Biological Chemistry. 257:12141-12147.
  12. Morel, J.L., Colin, F., Germon, J.C., Godin, P. and Juste, C. 1985. Methods for the evaluation of the maturity of minicipal refuse compost. In Composting of Agriculture and Other Waste, ed. J.K. Gasser. Elsevier Applied Science. New York, USA, pp. 56-72.
  13. Paik, C.H., Lee, M.G. and Kim, C.H. 1988. Studies on the correlation between germination Index and $CO_2$ emission for evaluation of the maturity of compost products. Journal of Animal Environmental Science. 4:183-191.
  14. Raposo, M.F.D.J. and Morais, R.M.S.C.D. 2011. Chlorella vulgaris as soil amendment: influence of encapsulation and enrichment with rhizobacteria. International Journal of Agriculture and Biology. 13:719-724.
  15. Sa, D.M. 2012. Biofertilizer development using indigenous micro-organisms for healthy seedling production. Report of Korea Institute of Plannung and Evaluation for Technology in Food and Agriculture. 20-52.
  16. Seo, U.K., Lee, J.W. and Ryoo, J.W. 2017. Effects of Chlorella culture solution using as midium of anaerobic digestate on early growth of Italian ryegrass (Lolium multiflorum L.). Journal of the Korean Society of Grassland and Forage Science. 36:393-401.
  17. Stirk, W.A., Balint, P., Tarkowska, D., Novak, O., Maroti, G., Ljung, K., Turecova, V., Strnad, M., Ordog, V. and J. van Staden. 2014. Effect of light on growth and endogenous hormones in Chlorella minutissima (Trebouxiophyceae). Plant Physiol. Biochemistry. 79:66-76. https://doi.org/10.1016/j.plaphy.2014.03.005
  18. Welbaum, G.E. and Bradford, K.J. 1991. Water relations of seed development and germination in muskmelon (Cucumis melo L.) VI. Influence of priming on germination responses to temperature and water potential during seed development. Journal of Experimental Botany. 42:393-399. https://doi.org/10.1093/jxb/42.3.393