• Journal of Plant Biotechnology
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• v.36 no.2
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• pp.163-169
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• 2009

Dendropanax morbifera (Araliaceae) is an endemic species in Korea and distributed in the southern part of Korea. The roots and stems of this plant have been used for folk medicine for the treatment of migraine headache, dysmenorrheal, and remove wind dampness and for Vanishes production. Production of adventitious roots in D. morbifera by in vitro cultures could be used as alternatives materials. Leaf, stem, and root segments from D. morbifera seedling were cultured on Murashige and Skoog (MS) medium supplemented with 3.0 mg/L IBA and 30 g/L sucrose. After 4 weeks of culture, the highest induction of adventitious roots was obtained from the leaf segment. Frequency of adventitious root formation on medium with various kinds of auxins (IAA, NAA, 2,4-D, and IBA) and various concentrations of IBA (0, 0.1, 0.5, 1.0, 3.0, and 5.0 mg/L) was tested. The maximum induction of adventitious root was obtained on medium with 1.0 mg/L IBA. In liquid culture, growth of root was best 1/2MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose. Adventitious roots were cultured in 5 L bioreactor containing 1/2 MS medium supplemented with 1.0 mg/L IBA and 30 g/L sucrose and mass-production of adventitious roots was successfully achieved. This study demonstrated for the first time to produce adventitious roots in D. morbifera.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.123-127
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• 2005

The evaluation of source potential and sink strength is the generally large and laborious sample size required to adequately assess anyone of the parameters in field-grown sweetpotato. For this purpose we used the rooted single-leaf cuttings with petioles, because the source and sink organs are restricted in this system. The rooted single-leaf cutting of sweetpotato provides a unique source-sink model system, and is established within about 50 days after planting. In this study, the sink potential of sweetpotato tubers was examined based on the expression of genes for starch synthesis (AGPase) and modification (SBEII and GBSSI) in single rooted leaf plant. The gene expression patterns of GBSSI, SBEII and AGPase at various developmental stages and in different types of root tissues presented. These results suggest that the rooted single-rooted method can be used an ideal model system to study physiological and biochemical mechanisms in sweetpotato.

• Journal of Ginseng Research
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• v.44 no.4
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• pp.552-562
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• 2020

Background: Ginsenosides are the unique and bioactive components in ginseng. Ginsenosides are affected by the growing environment and conditions. In New Zealand (NZ), Panax ginseng Meyer (P. ginseng) is grown as a secondary crop under a pine tree canopy with an open-field forest environment. There is no thorough analysis reported about NZ-grown ginseng. Methods: Ginsenosides from NZ-grown P. ginseng in different parts (main root, fine root, rhizome, stem, and leaf) with different ages (6, 12, 13, and 14 years) were extracted by ultrasonic extraction and characterized by Liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry. Twenty-one ginsenosides in these samples were accurately quantified and relatively quantified with 13 ginsenoside standards. Results: All compounds were separated in 40 min, and a total of 102 ginsenosides were identified by matching MS spectra data with 23 standard references or published known ginsenosides from P. ginseng. The quantitative results showed that the total content of ginsenosides in various parts of P. ginseng varied, which was not obviously dependent on age. In the underground parts, the 13-year-old ginseng root contained more abundant ginsenosides among tested ginseng samples, whereas in the aboveground parts, the greatest amount of ginsenosides was from the 14-year-old sample. In addition, the amount of ginsenosides is higher in the leaf and fine root and much lower in the stem than in the other parts of P. ginseng. Conclusion: This study provides the first-ever comprehensive report on NZ-grown wild simulated P. ginseng.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.4
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• pp.449-455
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• 2012

Greenhouse and laboratory experiments were conducted to determine the effects of shade treatment and substrate components on plant growth and physiological activity of Taraxacum officinale. Substrates combined with coco peat and perlite (ratio 70 : 30 and 50 : 50, v/v) showed higher growth and yield than their single substrates (p<0.05). Shade treatment also significantly reduced plant height, root length, root diameter, leaf area, chlorophyll content, and fresh weight (p<0.05), compared to no shade. Contents of total phenolics [mg chlorogenic acid equivalents (CAE) $kg^{-1}$ DW] and total flavonoids [mg naringin equivalents $kg^{-1}$ DW] showed higher amounts in shoot parts than root parts of T. officinale, with shade than no shade. The antioxidant potential of the methanol extracts from the plants dose-dependently increased. DPPH (1,1-diphenyl-2-picryl hydrazyl radical) free radical scavenging activity was higher in leaf parts than in root parts of the plants, and no shade than with shade.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.6
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• pp.1002-1007
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• 2010

The study was performed to investigate the influence on growth and development of grape-cuttings by arbuscular mycorrhizal (AM) fungi inoculation, AM colonization rate, and the phenomena of mycorrhizal association. Among the grape-cuttings, 'Kyoho' and 'Tamnara' cultivars inoculated with AM fungi showed significantly increase of leaf area, leaf number, total root length and root surface area than non-infected ones. But 'Cambell Early' did not showed any significant difference in total root length and root surface area even after the inoculation. The AM colonization rates in mycorrhizal inoculation treatment were 22.5-32.5% in total average after 8weeks, and were 29.6%, 28.8%, and 48.8% for 'Cambell Early', 'Tamnara', and 'Kyoho' respectively after 12weeks. The AM colonization rate marked very low level in non-colonization control plot.

• Korean Journal of Agricultural Science
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• v.42 no.4
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• pp.293-297
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• 2015

This study was conducted to evaluate the influence of feeding leaf positions on the growth, net formation of fruits, and occurrence of leaf yellowing symptoms (LYS) in muskmelon plants. Plants having five or ten more leaves above the fruit-bearing node produced the greater biomass than those of plants having equal or five less leaves above the fruit-bearing node. The number of leaves above the fruit-bearing node also influenced on the occurrence of LYS. The number of plants with LYS decreased as the number of leaves borne on the nodes above the fruit-bearing node increased. The LYS infected ratio of BL-5 treatment were the greatest, while fruit weight of BL+5 treatment were the greatest among all the tested treatments. In addition, the net formation of BL-5 treatment showed the poorest. Results indicated that maintaining the higher number of leaves over the fruit-bearing node might be feasible the practical method for coping physiological damages from yellowing symptoms.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48 no.2
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• pp.73-80
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• 2003

An experiment was carried out at the Field Laboratory of the Department of Crop Botany, Bangladesh Agricultural University, Mymensingh from March to July, 2001 to investigate the effect of biofertilizers on morpho-physiological characters of okra. The experiment was laid out in a randomized complete block design with four replications. There were nine treatments such as $\textrm{T}_0$ (control), $\textrm{T}_1$ (Azotobacter biofertilizer), $\textrm{T}_2$ (Azospirillum biofertilizer), $\textrm{T}_3$ (Azotobacter+Azospirillum biofertilizers), $\textrm{T}_4$ (Azotobacter+Cowdung 5 ton $\textrm{ha}^{-1}$), $\textrm{T}_5$ (Azospirillum+Cowdung 5 ton $\textrm{ha}^{-1}$), $\textrm{T}_6$(Azotobacter+Azospirillum+Cowdung 5 ton $\textrm{ha}^{-1}$), $\textrm{T}_7$ (Cowdung 5 ton $\textrm{ha}^{-1}$) and $\textrm{T}_8$ (60% Nitrogen). The experimental results revealed that significant variations exist among the treatments regarding morphological characters e.g. plant height, number of leaves/plant, stem base diameter, tap root length, and physiological characters like, root dry weight, leaf area index and crop growth rate. Number of leaves/plant, stem base diameter, root length, root dry weight, leaf area index and crop growth rate were found higher in $\textrm{T}_4$, $\textrm{T}_5$, $\textrm{T}_6$ and $\textrm{T}_8$ than the others. In all the parameters, $\textrm{T}_8$ gave the similar result with biofertilizers in combination with cowdung treatments and $\textrm{T}_7$ showed identical with $\textrm{T}_0$ (control). Biofertilizer treatments had insignificant effect on 1000-seed weight(g). Experimental results mentioned above revealed that morpho-physioligical characters of okra could be modified by the application of biofertilizer+cowdung. However, biofertilizers+Cowdung treatments were comparable to $\textrm{T}_8$(60% Nitrogen) in this study. This suggests that $\textrm{T}_4$ or $\textrm{T}_6$ or $\textrm{T}_5$ were more benificial in environmentally friendly okra cultivation and may be used as an alternative of inorganic nitrogen by saving cost of production and sustaining productivity.

• Journal of Korean Society of Forest Science
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• v.87 no.1
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• pp.50-56
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• 1998

Thirty-six $F_3$ hybrid poplar (Populus trichocarpa${\times}$P. deltoides) clones were fumigated with ozone to select for ozone sensitive and resistant clones. Fumigation was applied for 6 to 8 hours each day for approximately 3 months at ozone concentrations of 90 to 115 ppb using by open-top chambers. Height, diameter, number of leaves, total biomass, biomass components, root/shoot ratios, leaf drop and stomatal response were investigated. In summary, ozone generally reduced height, diameter, number of leaves, total biomass, and root/shoot ratios. Ozone stress induced leaf drop and foliar senescence in trees. This study showed very low relationship between total biomass and stomatal conductance. Increased plant resistant to ozone is not always correlated with stomatal behaviour. Probably, characterization of biochemical and other physiological responses to ozone exposure can provide a better understanding of tree response to ozone environment.

• Korean Journal of Plant Resources
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• v.14 no.3
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• pp.188-195
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• 2001

The anatomical characteristics of rhizome, lateral root, stem, leaf, petiole, ovary and leaf epidermis about Atractylodes japonica Koidz. ex Kitam. and A. macrocephala Koidz. were investigated to estimate these taxonomic values. The cross sections of stem and ovary were very similar to two species, therefore these characters were not useful for delimitation of two species, but the size and distribution numbers per 100${\mu}{\textrm}{m}$$^2$of oil cavites in rhizome, the shape of leaf and petiole in cross section, the size of leaf epidermal cell and distribution numbers per 100 ${\mu}{\textrm}{m}$$^2$of stomata were differ from two species, therefore these were thought to be useful characters for delimitation of two species.

• Korean Journal of Medicinal Crop Science
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• v.25 no.3
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• pp.160-164
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• 2017

Background: This study were performed to determine the effect of root pruning of Zizyphus jujuba var. inermis (Bunge) Rehder. Root cutting inhibit vegetative growth and promote reproductive growth as temporarily reducing growth, net assimilation, water potential of leaf and cytokinin level. Methods and Results: The root pruning was treated of the root cutting widths 50, and 80 ㎝ and the root cutting depths 10, and 20 cm. The amount of root pruning and the number of suckers were the highest in the root-pruning treatment at a width of 50 cm and a depth of 20 cm. The blooming time was from June 18 to 20, and no difference was observed in the blooming time among the root-pruning treatments. The number of flowers was rather higher in the root-pruning treatment at a width of 50 cm and a depth of 20 cm and at a width of 80 cm and a depth of 20 cm. The percentage of fruit setting was higher in the plants whose roots were pruned at a depth of 20 cm than in the untreated plants. The fruit size, fruit weight, and sugar content showed no difference among the root-pruning treatments. Conclusions: The results showed that percentage of fruit setting increased with root pruning, while no difference was observed in the growth and fruit quality of plants.