• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 공동 춘계학술대회
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• pp.96.2-96
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• 2001

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• Animal cells and systems
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• 제5권1호
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• pp.77-83
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• 2001

The present study was conducted to elucidate whether the expression level of poly(ADP-ribose) polymerase (PARP) is related to the ultraviolet radiation (UV)-induced apoptosis. After treatment of the mammalian cell lines HeLa S3 and Chinese hamster ovary (CHO) with 50 J/m2 UV, induction of apoptosis was determined by several means during 24 h post-incubation. Incidence of apoptosis was much lower in CHO than HeLa S3 cells based on the percentage of apoptotic cells in terms of morphological changes in nucleus or direct counting of viable cells and qualitative or quantitative DNA fragmentation. Interestingly, when the expression level of PARP was measured by western blotting, the amounts of PARP that was retained at each time point inversely correlated with the incidences of apoptosis in these cells. Concomitant with generation of the 85 kDa fragment, 116 kDa PARP disappeared in HeLa S3 within 6 h after UV treatment, whereas a fair amounts of 116 kDa band was still retained in CHO cells at 36 h post-incubation. This inverse relationship was also observed in the adaptive response system, in which cells weve treated with a high dose of UV after pretreatment with a low dose. As expected, typical adaptive responses appeared in CHO cells but not in HeLa cells, showing greater cell viability and lesser DNA fragmentation. During the adaptive response in CHO cells, PARP was expressed at much higher level compared to the single, high dose-treated cells. Interestingly, even though PARP was induced at 6 h post-incubation In both cell types, its expression was more prominent in CHO cells. Thus, our data indicate that the retained level of intact PARP against UV damage inversely correlates with incidence of apoptosis in mammalian cells, and also suggest that a machinery to protect the PARP degradation against UV damage exists in CHO but not in HeLa S3 cells.

• 생명과학회지
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• 제12권4호
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• pp.469-476
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• 2002

남미지역에 서식하는 Tabebuia avellanedae의 수피에서 동정된 천연 quinone계 물질인 $\beta$-lapachone은 DNA topoisomerase I 억제제 이외 다양한 약리학적 기능이 있을 것으로 추정되지만 그 기능이 명확하지 않다. $\beta$-lapachone의 생리활성 기전 해석의 일환으로 본 연구에서는 인체 전립선 DU-145 암세포주의 성장에 미치는 $\beta$-lapachone의 영향을 조사하였다. p-lapachone이 함유된 배지에서 자란 암세포들은 $\beta$-lapachone 처리 농도 의존적으로 성장이 억제되었으며, 이는 apoptosis가 유발된 세포에서 특징적으로 관찰되는 chromatin condensation 및 DNA fragmentation 현상을 유발하였고, DNA flow cytometry 분석결과 apoptotic-sub Gl기에 해당하는 세포들의 빈도도 증가되었다. 또한 poly (ADP-ribose) polymerase 및 $\beta$-catenin 단백질의 발현에서도 apoptosis 유발 특이적인 분해 현상을 보여주었으며, DU-145 전립선 암세포에서 $\beta$-lapachone에 의한 이러한 apoptosis의 유발에는 Bax의 발현증가에 따른 Bcl-2 발현의 감소가 중요한 역할을 할 고 있는 것으로 사료된다.

• 한국식품저장유통학회지
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• 제11권3호
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• pp.347-351
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• 2004

큰느타리 버섯의 기능성 식품으로서 활용도를 높이기 위해 큰느타리 버섯 자실체의 이화학적 성분을 분석한 결과는 다음과 같다. 큰느타리버섯 자실체의 일반성분 중 조단백질과 총당이 높게 나타났으며, 무기성분은 Mg, Na, Zn, Fe, Ca 및 Cu 순으로 높게 나타났다. 유리아미노산은 hypoproline이 주요 유리아미노산으로 나타났으며, 구성아미노산은 arginineof 509.03 mg$\%$, lycine이 410.53 mg$\%$, glutamic acid가 190.05mg$\%$, phenylalanine이 150.63 mg$\%$ 및 histidine이 139.12 mg$\%$순으로 높게 함유되어 있었다. 유기산 함량은 citric acid, oxalic acid, formic acid 및 malic acid 순이었으며, 유리당은 glucose가 가장 많았고, 다음으로 fructose, ribose, galactose, lactose, arabinose 및 maltose 순이었다.

• 동의생리병리학회지
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• 제18권6호
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• pp.1661-1665
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• 2004

Herba Patriniae(HP) has been known to exert anti-tumoral activity in Korea. However, its molecular mechanism, of action is not understood. In this study, we found that HP induced apoptosis in androgen-dependent prostate cancer DU145 cells as evidenced by DNA fragmentation and chromatine condensation in hoechst dye staining. Our data demonstrated that HP-induced apoptotic cell death was accompanied by activation of caspase-3 and cleavages of its substrates, poly(ADP-ribose) polymerase(PARP) in a time- and concentration-dependent manner. Taken together, these results suggest that HP induces the activation of caspase-3, degradation of PARP, and eventually leads to apoptotic cell death.

• 동의생리병리학회지
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• 제18권5호
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• pp.1374-1381
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• 2004

The water extract of Dohongsamul-tang(DHSMT)has been traditionally used for treatment of ischemic heart in oriental medicine. However, little is known about the mechanism by which the water extract of DHSMT rescues cells from these damages. Therefore, this study was designed to evaluate the protective effects of DHSMT on zinc-mediated cytotoxicity in H9c2 cardiomyoblast cells. This study demonstrates that treatment of H9c2 cells with zinc caused a decrease in cell viability in a dose dependent manner and a chromatin condensation. Zinc induced the cleavage of poly(ADP-ribose) polymerase (PARP). In addition, zinc induced the decrease of Bcl-2, as well as increase of Bak expression and mitochondrial dysfunction. Zinc-induced H9c2 cell death was remarkably prevented by the pretreatment of DHSMT with consistent suppression of the cleavage of poly(ADP-ribose) polymerase (PARP), mitochondrial dysfunction and the expression of Bak and Bcl-2. Taken together, the results suggest that zinc induced severe cell death in H9c2 cardiomyoblast cells via intracellular GSH(reduced glutathione) depletion and the protective effects of DHSMT against oxidative injuries may be achieved through modulation of mitochondrial dysfunction and scavenging of ROS(reactive oxygen species).

• 미생물학회지
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• 제23권3호
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• pp.203-208
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• 1985

Bacillu! thuringiensis var. krustaki 3ab k-3의 감온성치사돌연변이체에 대한 생물학적 성상의 변이성의 차이를 조사하고 유전적 특성을 규명하기 위하여 생화학적 성상검사, 항생제에 대한 감수성 조사, 용혈성 검사, 돌연변이체균주의 특수배지에서의 성장비교, Mouse에 대한 치사효과를 $28^{\circ}C$의 허용온도에서 조사하였다. Urease를 생산하지 못하는 돌연변이체는 ts-U154, ts-U601, ts-U602, ts-U603, ts-U604, t-U788 이었다. ts-U603만이 운동성이 상실되었고, ts-U154는 Salicin과 cellobiose을 분해하지 못했고, ribose를 분해하지 않는 균주는 ts-U603였다. 항생제에 대한 반응은 ampicillin, cephalothin, penicillin에는 모두 저항성을 나타냈으나, ts-U154 균주만이 감수성을 나타냈다. Fructus gardenia배지에서 색소를 생산하지 않은 것은 ts-U21, ts-U74, ts-U131과 ts-U154였다 가장 적은 용혈성을 나타내는 균주는 정상형고 ts-U602였고 가장 많은 용혈성을 나타내는 것은 ts-U603 (1:512 x) 이었다. Mouse 에 대한 치사효과는 전혀 없었다.

• 대한화학회지
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• 제43권6호
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• pp.670-675
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• 1999

Methanopterin의 pteridine부분과 관련있는 구조를 가지는 6-[1-(4-ribityl-anilino)ethyl]-1,3,7-trimethyllumazine (2)를 합성하였다. 4-Ribitylaniline 유도체는 D-ribose와 N-benzoyl-4-bromoaniIine (7)을출발 물질로 하여 여러 단계를 거쳐 합성되었다. 6-Acetyl-1,3,7-trimethyllumazine (4)는 Timmis 반응을 이용하여 4-amino-1,3-dimethyl-5-nitrosouracil (3)과 2,4-pentanedione을 반응시켜 얻을 수 있었다. 화합물 4는 $NaBH_4$에 의한 환원반응과 뒤이은 $SOCl_2$에 의한 염소화반응으로 6-(1-chloroethyl)-1,3,7-trimethyllumazine (6)으로 변환되었다. 화합물 6과 4-(2,3:4,5-di-O-isopropylidene-D-ribityl)aniline (13)의 친핵성 치환반응으로 6-[1-{4-(2,3:4,5-di-O-isopropylidene-D-ribityl)anilino}ethyl]-1,3,7-trimethyllumazine (14)가 합성되었다 목표 화합물 2는 산 촉매하에서 화합물 14를 가수분해하여 얻어졌다.

• 한국식품영양과학회지
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• 제18권2호
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• pp.199-204
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• 1989

오징어육속에 함유되어 있는 주요유리당 성분을 조사하고 이들 성분의 추출용매와 추출시간에 따른 변화를 조사하였다. 오징어육의 주요 유리당성분은 glucose, fructose ribose이며 이들의 양이 전체의 80%이상을 차지하였고 galactose, xylose, mannose도 소량 검출되었다. 추출조건에 따라서는 생오징어의 경우는 물로서 추출하는 것이, 건조오징어의 경우는 70%에탄올로 추출하는것이 유리당의 추출량을 증가시킬 수 있었다. 추출시간에 따른 유리당의 추출량은 추출온도 $100^{\circ}C$이하에서는 추출용매에 관계없이 $2{\sim}3$시간 추출한 것이 가장 높게 나타났고, $120^{\circ}C$의 고온에서는 추출 1시간에 가장 높고, 그 이후에서는 급속히 감소하는 현상을 보였다.

• Molecules and Cells
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• 제37권7호
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• pp.526-531
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• 2014

Human PARP family consists of 17 members of which PARP-1 is a prominent member and plays a key role in DNA repair pathways. It has an N-terminal DNA-binding domain (DBD) encompassing the nuclear localisation signal (NLS), central automodification domain and C-terminal catalytic domain. PARP-1 accounts for majority of poly-(ADP-ribose) polymer synthesis that upon binding to numerous proteins including PARP itself modulates their activity. Reduced PARP-1 activity in ageing human samples and its deficiency leading to telomere shortening has been reported. Hence for cell survival, maintenance of genomic integrity and longevity presence of intact PARP-1 in the nucleus is paramount. Although localisation of full-length and truncated PARP-1 in PARP-1 proficient cells is well documented, subcellular distribution of PARP-1 fragments in the absence of endogenous PARP-1 is not known. Here we report the differential localisation of PARP-1 Nterminal fragment encompassing NLS in PARP-$1^{+/+}$ and PARP-$1^{-/-}$ mouse embryo fibroblasts by live imaging of cells transiently expressing EGFP tagged fragment. In PARP-$1^{+/+}$ cells the fragment localises to the nuclei presenting a granular pattern. Furthermore, it is densely packaged in the midsections of the nucleus. In contrast, the fragment localises exclusively to the cytoplasm in PARP-$1^{-/-}$ cells. Flourescence intensity analysis further confirmed this observation indicating that the N-terminal fragment requires endogenous PARP-1 for its nuclear transport. Our study illustrates the trafficking role of PARP-1 independently of its enzymatic activity and highlights the possibility that full-length PARP-1 may play a key role in the nuclear transport of its siblings and other molecules.