Browse > Article
http://dx.doi.org/10.5352/JLS.2002.12.4.469

Up-regulation of Bax is associated with DNA topoisomerase I inhibitor β-lapachone-induced apoptosis in human prostate carcinoma cells  

공규리 (동의대학교 한의과대학 생화학교실)
최병태 (동의대학교 해부학교실 및 한의학연구소)
최영현 (동의대학교 한의과대학 생화학교실)
Publication Information
Journal of Life Science / v.12, no.4, 2002 , pp. 469-476 More about this Journal
Abstract
The DNA topoismerase I inhibitor $\beta$-lapachone, the product of a tree from South America, is known to exhibit various biological properties, however the mechanisms of which are poorly understood. In the present report, we investigated the effects of $\beta$-lapachone on the growth of human prostate carcinoma DU-145 cells. Upon treatment with $\beta$-lapachone, a concentration-dependent inhibition of cell viability was observed and cells developed many of the hallmark features of apoptosis, including condensation of chromatin and DNA fragmentation. Flow cytometry analysis confirmed that $\beta$-lapachone increased populations of apoptotic-sub Gl phase. In addition, proteolytic cleavages of poly (ADP-ribose) polymerase (PARP) and $\beta$-catenin protein were observed after treatment of $\beta$-lapachone. These apoptotic effects of $\beta$-lapachone in DU-145 cells were associated with marked induction of Bax protein, however the levels of Bcl-2 expression were decreased in a dose-dependent manner.
Keywords
$\beta$-lapachone; prostate carcinoma; apoptosis; Bax; Bcl-2;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Comparison of antibacterial and antifungal activities of lapachol and β-lapachone /
[ Guiraud,P.;R.Steiman,G.M.Campos-Takati;F.Seigle-Murandi;M.Simeon de buocherg ] / Planta Med.   DOI   ScienceOn
2 β-lapachone-mediated apoptosis in human promyelocytic leukemia (HL-60) and human prostate canver cells:a p53-independent response /
[ Planchom,S.M.;S.Wuerzberger;B.Frydman;D.T.Witiak;P.Hutson;D.R.Church;G.Wilding;D.A.Boothman ] / Cancer.Res.
3 Topoisomerase-I inhibitors for human malignant glioma:differential modulation of p53,P21 bax and bcl-2 experssion and of CD95-mediated apoptosis by camptothecin and β-lapachone /
[ Weller,M.;S.Winter;C.Schmidt;P.Esser;A.Fontana;J.Dichgans;P.Groscurth ] / Int.J.Cancer   DOI   ScienceOn
4 Apoptosis by death factor /
[ Nagata,S ] / Cell   DOI   ScienceOn
5 Phosphatiedylserine externaliztion during CD95-induced apopotsis of cells and cytoplasts ICE/CED-3 protease activity /
[ Martin,S.J.;D.M.Finucane;G.P.Amarante-Mendes;G.A.O'Brien;D.R.Green ] / J.Biol.Chem.   DOI   ScienceOn
6 p53-independent induction of p21(WAF1/CIP1),reduction of cyclin in human prostate carcinoma cells /
[ Choi,Y.H.;W.H.Lee;K.Y.Park;L.Zhang ] / Jpn.J.Cancer Res.   DOI
7 Mitochondria, Oxidative stress, and antioxidant defences /
[ Lenaz,G.;C.Bovina;G.Formiggini;G.P.Gastelli ] / Acta.Biochem.Pol.
8 β-lapachone induced cell death in human hepatoma(HepA2)cells /
[ Lai.C.C.;T.J.Liu;L.K.Ho;M.J.Don;Y.P.Chau ] / Histol.Histopathol
9 Permature p34cdc2 activation required for apoptosis /
[ Shi,L.;W.K.Nishioka,J.Th'ng;E.M.Bardury;D.W.Litchfield;A.H.Greenberg ] / Science   DOI
10 Phosphorylation of p53, induction of Bax and activation of caspases during β-lapachoine- mediated apoptosis in human prostate epithelial cells /
[ Choi,Y.H.; M.J. Kim;S.Y.Lee;Y.N.Lee;G.Y.Chi;H.S.Eom;N.D.Kim;B.T.Choi ] / Int.J.Oncol.
11 Induction of CDK inhibitors (p21(WAF1)) and Bak in the β-lapachone-induced apoptosis of human prostate cancer cells /
[ Don,M.J.;Y.H.Chang;K.K.Chen;L.K.Ho;Y.P.Chau ] / Mol.Pharmacol.
12 The Bcl-e protein family /
[ Antonsson,B.;J.C.Martinou ] / Exp.Cell.Res.   DOI   ScienceOn
13 Induction of apoptosis in MCF-7:WS8 breast cancer cells by β-lapachone /
[ Wuerzberger,S.M.;J.J.Pink;S.M.Planchon;K.L.Byers;W.G.Bornmannn;D.A.Boothman ] / Cancer.Res.
14 Apoptosis-induced cleavage of β-catenin by caspase-3 results in proteolytic fragments with reduced tran-sacrivation potentail /
[ Steinhusen,U.;V.Badock;A.Bauer;J.Behrens;B.Wittman-Liebold;B.Dorken;K.Bommnet ] / J.Biol.Chem.   DOI   ScienceOn
15 β-lapachone induces cell cycle arrest and apoptosis in human colon cancer cells /
[ Huang.L;A.B.Pardee ] / Mol.Med.
16 Multople pathways ro apoptosis /
[ Evans,V.G ] / Cell.Biol.Int.   DOI   ScienceOn
17 Induction of p21(WAF-1/CIPI) during differentiation /
[ Steinman,R.A.;B.Hoffman;A.Iro;C.Guilouf;D.A.Lievermann;M.E.E1-Houseini ] / Oncogene
18 Cleavage of poly ADP-ribose polymerase by a proteinase with properties like ICE. /
[ Lazebnik,Y.A.;S.H.Kaufmann;S,Desnoyers;G.G.Poirier;W.C.Earnshaw ] / Nature   DOI   ScienceOn
19 Apoptosis and programmed cell death immunity /
[ Cohen,J.J.;R.C.Duke;V.A.Fadok;K.S.Sellins ] / Annu.Rev.Immunol   DOI   ScienceOn
20 Effects of β-lapachone, a peroxide-generation quinone, on macrolecue synthesis and degration in Try-panosoma cruzi /
[ Goijman,S.G.;A.O.Stoppani ] / Arch.Biochem.Biophys   DOI   ScienceOn
21 Activation of c-Jin NH2-terminal kinase and subsequent CPP32/Yama during topoisomerase inhiitor β-lapachone -induced apoptosis through an oxidation -dependent pathway /
[ Shiah.S.G.;S.E.Chuang;Y.P.Chau.S.C.Shen;M.L.Kuo ] / Cancer.Res.
22 Regulation of cyclin D1 by calpain prostease /
[ Choi,Y.H.;S.J.Lee;P.Nguyen;J.S.Jang;J.Lee;M.L.Wu;E.Takano;M.Maki;P.A.Henkart;J.B.Trepel ] / J.Biol.Chem.   DOI   ScienceOn
23 β-lapachone enhancement of lipid peroxidation and superoxide anion and gydrogen peroxide formation by sarcoma 180 ascites tumor cells /
[ Dacampo.R.;F.S.Cruz;A.Boveris;R.P.Muniz;D.M.Esquivel ] / Biochem.Pharmacol.   DOI   ScienceOn
24 Induction of apotosis by ursolic acid through activation of caspases and down-regulation of c-IAPs in human prostate epithelial cells /
[ Choi,Y.H.;J.H.Baek;M.A.Yoo;H.Y.Chung;N.D.Kim;K.W.Kim ] / Int.J.Oncol.
25 Cell survical and death programmes /
[ Chiarugi,V.;L.Magnelli;A.Turchetti;M.Cinelli;S.Cavari;M.Ruggiero ] / Pharmacol.Res.   DOI   ScienceOn
26 Yama/CPP32, a mammalian homolog of CED-3,is a CrmA-inhibitable protease that cleaver the death suvstrate poly(ADP-ribose) polymerase /
[ Tewari.M.;L.T.Quan;K.O'Rourke;S.Desnoyers,Z.Zeng;D.R.Beidler;G.G.Poirier;G.S.Salvesen;V.M.Dixit ] / Cell   DOI   ScienceOn
27 Apoptotic activity of novel bile acid derivatives in human leukemic T cells through the activation of caspases /
[ Choi,Y.H.;E.O.Im;H.S.Suh;Y.E.Jin;W.H.Lee;Y.H.Yoo;K.W.Kim;N.D.Kim ] / Int.J.Oncol.
28 Tissue-specifiec Bcl-2 protein partners in apoptosis:An ovarian paradigm /
[ Hsu.S.Y.;A.J.Hsueh ] / Physiol.Rev.
29 Bcl-2 protects against β-lapachone-mediated caspase 3 activation and apoptosis in gyman myeloid leukemia(HL-60) cells /
[ Planchom,S.M.;S.Wuerzberger-Davis; J.J.Pink;K.A. Robertson;W.G.Robertson;D.A.Boothman ] / Oncol.Rep.
30 β-lapachone :synthesis of derivatives and activities in tumor models /
[ Schaffner-Sabba,K.;K.H.Schmidt-Ruppin;W.Wehrli;A.R.Schuerch;J.W.Wasley ] / J.Med.Chem.   DOI
31 Induction of apoptosis by β-lapachone in human prostate canver cells /
[ Li,C.J.;L.Wang;A.B.Pardee ] / Cancer.Res.
32 Apoptosis.The role of the endonuclease /
[ Arends,M.J.;R.G.Morris;A.H.Wylli ] / Am.J.Pathol
33 Cell adhesion molecules in the development and progression of malignant melanoma /
[ Johnson,J.P. ] / Cancer Metastasis.Res.   DOI   ScienceOn
34 Bcl-2 prolongs cell survival after Bax-induced release of cytochrome c. /
[ Rosse,T.;R.Olivier;L.Monney;M.Rager;S.Conus;I.Fellay;B.Jansen;C.Broner ] / Nature   DOI   ScienceOn
35 β-Lapachone, a novel DNA topoisomerase I inhibitor with a mode of action different from camptothecin /
[ Li,C.J.;L.Averboukh;A.B.Pardee ] / J.Biol.Chem.
36 Apoptosis-associated cleavade of β-catenin in human colon cancer and rat hepatoma cells /
[ Fukuda.K ] / Int. J.Biochem.Cell.Biol   DOI   ScienceOn
37 p53 is required for radiation -induced apoptosis in mouse thymocytes /
[ Lowe,S.W.;E.M.Schmitt;S.W.Smith;B.A.Osborme;T.Jacks ] / Nature   DOI   ScienceOn
38 In vitro and in vivo evaluation of the toxicity of 1,4-napthoquinone and 1,2--naphthoquinone derivatives against Trypansoma cruzi /
[ Lopez,J.N.;F.S.Cruz;R.Docampo.;M.E.Vascpmcellos;M.C.Sampaio;A.V.Pinto;B.Gilbert ] / Ann.Trop.Med.Parasitol
39 Specific proteolytic cleavage of poly(ADP-ribose) polymerase:an early marker of chemotherapy-induced apoptosis /
[ Kaufmann,S.H.;S.Desnoyers;Y.Ottaviano;N.E.Davidson;G.G.Poirier ] / Cancer.Res.
40 E-cadherin-catenin cell-cell adhesion complex and human cancer /
[ Wijnhoven,B.P;W.N.Dinjens;M.Pignatelli ] / Br.J.Surg   DOI   ScienceOn
41 The topoisomerase I inhibitors,camptothecin and β-lapachone, induce apoptosis of human retinal pigment epithelial cells /
[ Hueber,A.;P.Esser,K.Heimann;N.Kociok;S.Winter;M.Weller ] / Exp.Eye.Res.   DOI   ScienceOn
42 Cadherins;crucial regulators of structure and fuction on reproductive tissues /
[ Rowlands,T.M.J.M.Symonds,R.Farookhi;O.W.Blaschuk ] / Rev.Reprod   DOI   ScienceOn
43 Induction of bax by genotoxic stress in human cells correlates with normal p53 status and apoptosis /
[ Zhan,Q.;S.Fan;I.Bae;C.Guillouf;D.A.Liebermann;P.M.O'Connor;A.J.Jr.Fornace ] / Oncogene
44 Activation of a cysteine protease in MCF-7 and T47D brest cancer cells during β-lapachone-mediated apoptosis /
[ Pink,J.J.;S.Wuerzberger-Davis;C.Tagliarino;S.M.Planchon;X.Yang;C.J.Froelich:D.A.Boothman ] / Exp.Cell.Res.   DOI   ScienceOn