Apoptosis-Inducing Effect of Herba Patriniae Extract in Androgen Independent Prostate Cancer DU145 Cells

남성호르몬 비의존형 전립선 암세포에서 패장 추출물의 세포고사 유도 효과

  • Kwon Kang Beom (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kim Eun Kyung (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Ryu Cheal In (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Park Hyung Kwon (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Seong Ki Ho (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Song Je Moon (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Lee Kyung Yong (Department of Physiology, School of Oriental Medicine, Wonkwang University) ;
  • Kwon Young Dal (Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Wonkwang University) ;
  • Seo Eun A (Department of Food and Nutrition, School of Human Envioronmental Science, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, School of Oriental Medicine, Wonkwang University)
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 김은경 (원광대학교 한의과대학 생리학교실) ;
  • 류철인 (원광대학교 한의과대학 생리학교실) ;
  • 박형권 (원광대학교 한의과대학 생리학교실) ;
  • 성기호 (원광대학교 한의과대학 생리학교실) ;
  • 송제문 (원광대학교 한의과대학 생리학교실) ;
  • 이경용 (원광대학교 한의과대학 생리학교실) ;
  • 권영달 (원광대학교 한의과대학 재활의학교실) ;
  • 서은아 (원광대학교 생활과학대학 식품영양학과) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2004.12.01

Abstract

Herba Patriniae(HP) has been known to exert anti-tumoral activity in Korea. However, its molecular mechanism, of action is not understood. In this study, we found that HP induced apoptosis in androgen-dependent prostate cancer DU145 cells as evidenced by DNA fragmentation and chromatine condensation in hoechst dye staining. Our data demonstrated that HP-induced apoptotic cell death was accompanied by activation of caspase-3 and cleavages of its substrates, poly(ADP-ribose) polymerase(PARP) in a time- and concentration-dependent manner. Taken together, these results suggest that HP induces the activation of caspase-3, degradation of PARP, and eventually leads to apoptotic cell death.

Keywords

References

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