• Title/Summary/Keyword: resistance model

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Control Indian meal moth Plodia interpunctella by gas treatment

  • Han, Gyung Deok;Kwon, Hyeok;Jin, Hyun Jung;Kum, Ho Jung;Kim, Bo Hwan;Kim, Wook
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.45-45
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    • 2017
  • The Indian meal moth, Plodia interpunctella, is one of the most important pests of stored food in the food processing industry worldwide. To control the Indian meal moth, methyl bromide, phosphine, high carbon dioxide, sulfuryl fluoride and plant essential oil fumigation have been considered. However, these treatments have disadvantages. For example, depleting the ozone layer, showing resistance in insect, low control efficacy or need high cost for treatment. Chlorine dioxide ($ClO_2$) is strong disinfectant and insecticide. The gas caused a malfunction in enzymes. The oxidative stress induced by $ClO_2$ gas treatment damaged to a physiological system and all life stages of P. interpunctella. The gaseous $ClO_2$ is a convincing alternative to methyl bromide for controlling P. interpunctella. The gaseous $ClO_2$ was generated by a chlorine dioxide generator (PurgoFarm Co., Ltd., Hwasung, Korea). It generated highly pure $ClO_2$ gas and the gas blown out through a vent into a test chamber. Gas entry to the chamber was automatically controlled and monitored by a PortaSene II gas leak detector (Analytical Technology, Collegeville, PA, USA). The properly prepared eggs, larvae, pupae, and adults of P. interpunctella were used in this experiment. Data were analyzed using SAS 9.4. Percentage data were statistically analyzed after arcsine-root transformation. Analysis of variance was performed using general linear model, and means were separated by the least significant difference test at P < 0.05. Fumigation is an effective management technique for controlling all stages of P. interpunctella. We found that $ClO_2$ gas treatment directly effects on egg, larvae, pupae and adults of P. interpunctella. The gas treatment with proper concentration for over a day achieved 100 % mortality in all stages of P. interpunctella and short time treatment or low concentration gas treatment results showed that the egg hatchability, pupation rate, and adult emergency rate were lowered compare with untreated control. Also, abnormal pupae or adult rate were increased. Gaseous $ClO_2$ treatment induced insecticidal reactive oxygen species (ROS), and it resulted in fatal oxidative stress in P. interpunctella. Taken together, these results showed that exposure proper concentration and time of the gas control all stages of P. interpunctella by inducing fatal oxidative stress. Further studies will be required to apply the gas treatment under real-world condition and to understanding physiological reaction in P. interpunctella caused by oxidative stress.

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Primary Culture and Characteristics of Blood-Brain Barrier Endothelial Cells from Rat Brain Microvessel (쥐의 뇌 미세혈관 내피세포를 이용한 뇌혈관장벽 내피세포의 배양과 특성)

  • Lee, Hee-Sang;Kim, Seok-Jung;Kim, Dae-Jin;Chung, Yoon-Hee;Kim, Sung-Su;Lee, Won-Bok;Kim, Kyung-Yong
    • Applied Microscopy
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    • v.35 no.4
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    • pp.55-63
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    • 2005
  • The characteristics of primary cultured rat brain microvessel endothelial cells (RBMECs) were studied using microscopy, immunohistochemistry and measuring of transendothelial electrical resistance (TER). The RBMECs formed a monolayer by $5{\sim}6$ days after plating and showed characteristics of whirling appearance. The TER increased until day 5 and decreased then. There was few immunoreaction with anti-GFAP, anti-GalC, anti-neurofilament 160/200 kD antibodies. So the contamination of astrocyte, oligodendrocyte, and neuron. could be ruled out.. Immunoreaction to vWF antigen was widespread througout the cytoplasm as Weibel-Palade granule. Immunoreaction to tight junction proteins, i.e. occludin, ZO-1, and ZO-2 was seen at cell contact. In summary, RBMECs isolated and cultured showed morphological, immunohistochemical and electrical characteristics of blood-brain barrier (BBB). The in vitro BBB model can be used in studying characteristics of in vivo BBB.

Long-Term Performance Evaluation of Concrete Utilizing Oyster Shell in Lieu of Fine Aggregate (굴패각을 잔골재로 대체 사용한 콘크리트의 장기성능 평가)

  • Yang, Eun-Ik;Yi, Seong-Tae;Kim, Hak-Mo;Shim, Jae-Seol
    • Journal of the Korea Concrete Institute
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    • v.15 no.2
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    • pp.280-287
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    • 2003
  • To evaluate the practical application of oyster shells(OS) as construction materials, an experimental study was performed. More specifically, the long-term mechanical properties and durability of concrete blended with oyster shells were investigated. Test results indicate that long-term strength of concrete blended with 10% oyster shells is almost identical to that of normal concrete. However, the long-term strength of concrete blended with 20% oyster shells is appreciably lower than that of normal concrete. Thereby, concrete with higher oyster shell blend has the possibility of negatively influencing the concrete long-term strength. Elastic modulus of concrete blended with crushed oyster shells decreases as the blending mixture rate increases. Namely, the modulus is reduced to approximately 10∼15% when oyster shells are blended up to 20% as the fine aggregate. The drying shrinkage strain increases with an increasing crushed oyster shells substitution rate. In addition, the existing model code of drying shrinkage and creep do not coincide with the test results of this study. An adequate prediction equation needs to be developed. The utilization of oyster shells as the fine aggregate in concrete has an insignificant effect on fleering and thawing resistance, carbonation and chemical attack of concrete. However, water permeability is considerably improved.

Analysis of Frequency Response Curve for Conduction-Cooled Power Capacitors (전도 냉각 파워 커패시터의 주파수 응답 곡선 분석)

  • An, Gyeong Moon;Kim, Hiesik
    • Journal of the Institute of Electronics and Information Engineers
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    • v.53 no.10
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    • pp.123-130
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    • 2016
  • High-frequency induction heating equipment can heat the metal by applying a High-Frequency power to the resonant circuit. The resonance circuit is composed of the work coil and the conduction-cooled power capacitor, it influences the performance of the heat treatment equipment according to the characteristics of the capacitor. However, dependence on conduction-cooled power capacitor's import is high due to lack of core technology research and development. Minimizing the generation of internal heat transmitted inside during LC resonance, reduce the reactive power loss, there is a need for a capacitor within the voltage characteristic outstanding. To implement localization it is vital that prior study of the analysis on the frequency response characteristic for the finished capacitor advanced manufacturer be implemented. Studying the interpolation method to read the value at any point of the characteristic curve for a given log-log scale was applied to the analysis tool of the capacitor by my proposed algorithm. The simulation for reproducing frequency response curves was attempted by assuming a capacitor in a simplified series equivalent RC circuit to obtain the equivalent series resistance value. It was confirmed that the reproduction rate was the result value above 83% as compared to the simulation of the properties and characteristics on the actual reactive power for Peak value, and that the algorithm can be applicable when analyzing and predicting the characteristic curves of a simpled model capacitor.

Evaluation of the Stability of Quay Wall under the Earthquake and Tsunami (지진 및 지진해일파 작용하의 해안안벽의 안정성평가)

  • Lee, Kwang-Ho;Ha, Sun-Wook;Lee, Kui-Seop;Kim, Do-Sam;Kim, Tae-Hyung
    • Journal of the Korean Geotechnical Society
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    • v.27 no.3
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    • pp.41-54
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    • 2011
  • The present study analyzes the stability of waterfront quay wall under the combined action of earthquake and tsunami. Adopting the limit equilibrium method, the stability of waterfront quay wall is checked for both the sliding and overturning. Forces due to tsunami are compared with the proposed formula and the 3-D one-field Model for immiscible TWO-Phase flows (TWOPM-3D). Variations of the stability of wall are also proposed by the parametric study including tsunami water height, horizontal seismic acceleration coefficient, internal friction angle of soil, friction angle between the wall and the soil and the pore water pressure ratio. The present study about the stability of wall is also compared with the case when earthquake and tsunami are not considered. As a result, the result of numerical analysis about the tsunami force is similar to that of proposed formula. When earthquake and tsunami are simultaneously considered, the stability of wall in passive case significantly decreases and tsunami forces in active case are affected as a resistance force on the wall and so the stability of wall increases.

Seismic Behavior and Estimation for Base Isolator Bearings with Self-centering and Reinforcing Systems (자동복원 및 보강 시스템과 결합된 면진받침의 지진거동과 평가)

  • Hu, Jong Wan
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.35 no.5
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    • pp.1025-1037
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    • 2015
  • Flexible base isolation bearings that separate superstructure from ground have been widely used in the construction field because they make a significant contribution to increasing the fundamental period of the structure, thereby decreasing response acceleration transmitted into the superstructure. However, the established bearing devices installed to uphold the whole building give rise to some problems involved with failure and collapse due to lack of the capacity as modern structures are getting more massive and higher. Therefore, this study suggests new isolation bearings assembled with additional restrainers enabled to reinforcing and recentering, and then evaluates their performance to withstand the seismic load. The superelastic shape memory alloy (SMA) bars are installed into the conventional lead-rubber bearing (LRB) devices in order to provide recentering forces. These new systems are modeled as component spring models for the purpose of conducting nonlinear dynamic analyses with near fault ground motion data. The LRB devices with steel bars are also designed and analyzed to compare their responses with those of new systems. After numerical analyses, ultimate strength, maximum displacement, permanent deformation, and recentering ratio are compared to each model with an aim to investigate which base isolation models are superior. It can be shown that LRB models with superelastic SMA bars are superior to other models compared to each other in terms of seismic resistance and recentering effect.

Test methodology of acceleration life test on feeder cable assembly (Feeder Cable Assembly의 가속수명시험법 개발)

  • Han, Hyun Kak
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.8
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    • pp.62-68
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    • 2016
  • The feeder cable assembly is an automotive part used for telecommunication. If it malfunctions, the control and safety of the automobile can be put at risk. ALT (Accelerated Life Testing) is a testing process for products in which they are subjected to conditions (stress, strain, temperatures, etc.) in excess of their normal service parameters in an attempt to uncover faults and potential modes of failure in a short amount of time. Failure is caused by defects in the design, process, quality, or application of the part, and these defects are the underlying causes of failure or which initiate a process leading to failure. Thermal shock occurs when a thermal gradient causes different parts of an object to expand by different amounts. Thermal shock testing is performed to determine the ability of parts and components to withstand sudden changes in temperature. In this research, the main causes of failure of the feeder cable assembly were snapping, shorting and electro-pressure resistance failure. Using the Coffin-Manson model for ALT, the normal conditions were from Tmax = $80^{\circ}C$ to Tmin = $-40^{\circ}C$, the accelerated testing conditions were from Tmax = $120^{\circ}C$ to Tmin = $-60^{\circ}C$, the AF (Acceleration Factor) was 2.25 and the testing time was reduced from 1,000 cycles to 444 cycles. Using the Bxlife test, the number of samples was 5, the required life was B0.04%.10years, in the acceleration condition, 747 cycles were obtained. After the thermal shock test under different conditions, the feeder cable assembly was examined by a network analyzer and compared with the Weibull distribution modulus parameter. The results obtained showed good results in acceleration life test mode. For the same reliability rate, the testing time was decreased by a quarter using ALT.

CHANGES OF ABUTMENT SCREW AFTER REPEATED CLOSING AND OPENING

  • Kim Hee-Jung;Chung Chae-Heon;Oh Sang-Ho;Choi Han-Cheol
    • The Journal of Korean Academy of Prosthodontics
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    • v.42 no.6
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    • pp.628-640
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    • 2004
  • Statement of problem. Wear as a result of repeated closing/opening cycles may decrease the friction coefficient of screw head, threads, and other mating components and, consequently, resistance to opening gradually decreases. It may cause screw loosening, which is one of the most common failures in implant prosthesis. Purpose. The purpose of this study is to evaluate the changes on the head and thread surface of the abutment screws after repeated closing and opening through the examination of tested screws in SEM(scanning electron microscope). Materials and methods. Five species of abutments were selected (3i-three, Avana-two) respectively by two pieces. The implant fixtures were perpendicularly mounted in liquid unsaturated polyesther(Epovia, Cray Valley Inc.) with dental surveyor. Each abutment was secured to the implant fixture by each abutment screw with recommended torque value using a digital torque controller. The abutment screws were repeatedly tightened and removed 20 times with a digital controller. FESEM (field emission scanning electron microscope, Netherland, Phillips co., model:XL 30 SFEG) was used to observe changes of each part caused by repeatedly closing/opening expeiment. First, the Photomicrographs of pre-test screws provided by each manufacturer were taken. The changes of each screw were investigated after every fifth closing and opening experiment with FESEM. Scaning electron microscope photomicrographs of each screw were taken four times. Results. As the number of closing and opening was increased, the wear or distortion of hexed or squared slot that contacted with the driver tip was more severely progressed. Wear or distortion of hexed slot was more severe than that of squared slot and it was more remarkable in the titanium screw than in the gold screw. All the tested screws showed that the width in the crest of their screw thread decreased gradually as the test was proceeded. Conclusions. Conclusively, we recommend the clinical use of gold screw, a periodic exchanges of abutment screws and avoiding repeated closing/opening unnecessarily. We also suggest a more careful manipulation of the abutment screw and screw-driver and using of abutment screw with an acute-angled slot design rather than an obtuse-angled one. Finally, it is suggested that the new slot design and the surface treatment for enduring wear or distortion should be devised.

Evaluation of Interlayer Shear Properties and Bonding Strengths of a Stress-Absorbing Membrane Interlayer and Development of a Predictive Model for Fracture Energy (덧씌우기 응력흡수층에 대한 전단, 부착강도 평가 및 파괴에너지 예측모델 개발)

  • Kim, Dowan;Mun, Sungho;Kwon, Ohsun;Moon, Kihoon
    • International Journal of Highway Engineering
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    • v.20 no.1
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    • pp.87-95
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    • 2018
  • PURPOSES : A geo-grid pavement, e.g., a stress-absorbing membrane interlayer (SAMI), can be applied to an asphalt-overlay method on the existing surface-pavement layer for pavement maintenance related to reflection cracking. Reflection cracking can occur when a crack in the existing surface layer influences the overlay pavement. It can reduce the pavement life cycle and adversely affect traffic safety. Moreover, a failed overlay can reduce the economic value. In this regard, the objective of this study is to evaluate the bonding properties between the rigid pavement and a SAMI by using the direct shear test and the pull-off test. The predicted fractural energy functions with the shear stress were determined from a numerical analysis of the moving average method and the polynomial regression method. METHODS : In this research, the shear and pull-off tests were performed to evaluate the properties of mixtures constructed using no interlayer, a tack-coat, and SAMI with fabric and without fabric. The lower mixture parts (describing the existing pavement) were mixed using the 25-40-8 joint cement-concrete standard. The overlay layer was constructed especially using polymer-modified stone mastic asphalt (SMA) pavement. It was composed of an SMA aggregate gradation and applied as the modified agent. The sixth polynomial regression equation and the general moving average method were utilized to estimate the interlayer shear strength. These numerical analysis methods were also used to determine the predictive models for estimating the fracture energy. RESULTS : From the direct shear test and the pull-off test results, the mixture bonded using the tack-coat (applied as the interlayer between the overlay layer and the jointed cement concrete) had the strongest shear resistance and bonding strength. In contrast, the SAMI pavement without fiber has a strong need for fractural energy at failure. CONCLUSIONS : The effects of site-reflection cracking can be determined using the same tests on cored specimens. Further, an empirical-mechanical finite-element method (FEM) must be done to understand the appropriate SAMI application. In this regard, the FEM application analy pavement-design analysis using thesis and bonding property tests using cored specimens from public roads will be conducted in further research.

Histopathologic Features and CD5+ B-lymphocyte Expression in the Experimental Allergic Neuritis (실험적 자가면역성 말초신경염에서의 조직병리적 병변 및 CD5+ B-림프구의 발현)

  • Cho, Joong-Yang;Choi, Won-Jun;Kim, Sung-Hun;Sung, Jung-Joon;Kim, Ho-Jin;Park, Kyung-Seok;Choi, Ki-Young;Kim, Hyun-Jung;Lee, Kwang-Woo
    • Annals of Clinical Neurophysiology
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    • v.1 no.2
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    • pp.91-98
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    • 1999
  • Background : The pathogenesis of acute inflammatory demyelinating polyradiculoneuropathy (AIDP), Guillain Barre syndrome (GBS) is not clear, but it has been known that the immune mechanisms play an important role. Authors performed this study to establish an animal model of experimental allergic neuritis (EAN) by immunizing the myelin components of peripheral nerves and to understand the electrophysiological and histopathological features as well as the ${CD_5}^+$ B-lymphocyte changes in peripheral bloods in the EAN models. Methods : Lewis rats weighing 150-200 gm were injected subcutaneously in soles two times with total myelin, P0, P1, or P2 proteins purified from the bovine cauda eguina. The EAN induction was assessed by evaluating clinical manifestations. The electrophysiological and histopathological features were studied as routine methods. The ${CD_5}^+$ Blymphocytes were double stained using monoclonal FITC conjugated anti-rat CD45RA and R-PE conjugated anti-rat ${CD_5}^+$ antibodies and calculated using a fluorescence activated cell sorter (FACS). Results : The EAN animal models were established. In two out of five, in one out of two, in none out of three, and in none out of one Lewis rats injected with purified total myelin, P0, P1, P2 proteins respectively, They showed slow spontaneous motor activity and weak resistance against pulling back by tails. The typical electrophysiological and histologic findings in total protein and P0 induced EAN animal models were the decreased conduction velocity, the decreased compound muscle action potential (CMAP) amplitude and the dispersion phenomenon. The perivascular infiltrates of lymphocytes with focal demyelinating process were found in light microscopy. The ${CD_5}^+$ B-lymphocyte expression in three EANs were 2.38%, 3.50% 2.50%, which were not significantly increased, compared with those in normal controls. Conclusion : The EAN animal models were successfully established by injecting the total myelin and P0 myelin and they showed electrophysiological and histological features typical of demyelinating process. However they did not show an increased expression of ${CD_5}^+$ B-lymphocyte in peripheral bloods which could be indirect evidence of humoral autoimmunity.

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