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Primary Culture and Characteristics of Blood-Brain Barrier Endothelial Cells from Rat Brain Microvessel  

Lee, Hee-Sang (Department of Anatomy, College of Medicine, Chung-Ang University)
Kim, Seok-Jung (Department of Anatomy, College of Medicine, Chung-Ang University)
Kim, Dae-Jin (Department of Anatomy, College of Medicine, Chung-Ang University)
Chung, Yoon-Hee (Department of Anatomy, College of Medicine, Chung-Ang University)
Kim, Sung-Su (Department of Anatomy, College of Medicine, Chung-Ang University)
Lee, Won-Bok (Department of Anatomy, College of Medicine, Chung-Ang University)
Kim, Kyung-Yong (Department of Anatomy, College of Medicine, Chung-Ang University)
Publication Information
Applied Microscopy / v.35, no.4, 2005 , pp. 55-63 More about this Journal
Abstract
The characteristics of primary cultured rat brain microvessel endothelial cells (RBMECs) were studied using microscopy, immunohistochemistry and measuring of transendothelial electrical resistance (TER). The RBMECs formed a monolayer by $5{\sim}6$ days after plating and showed characteristics of whirling appearance. The TER increased until day 5 and decreased then. There was few immunoreaction with anti-GFAP, anti-GalC, anti-neurofilament 160/200 kD antibodies. So the contamination of astrocyte, oligodendrocyte, and neuron. could be ruled out.. Immunoreaction to vWF antigen was widespread througout the cytoplasm as Weibel-Palade granule. Immunoreaction to tight junction proteins, i.e. occludin, ZO-1, and ZO-2 was seen at cell contact. In summary, RBMECs isolated and cultured showed morphological, immunohistochemical and electrical characteristics of blood-brain barrier (BBB). The in vitro BBB model can be used in studying characteristics of in vivo BBB.
Keywords
BBB; Endothelial cell; Tight junction;
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