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Research about Urban Growth Model's Automation (도시성장모형의 시뮬레이션 자동화에 관한 연구)

  • Yun, Jeong-Mi;Park, Jeong-Wo
    • Journal of the Korean Association of Geographic Information Studies
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    • v.11 no.1
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    • pp.1-9
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    • 2008
  • Recently, various researches have been studied on the predict method of land change according to its development. The Cellular Automata(CA) is one of the most popular methods in the urban growth modeling. The basis principle of CA is to repeat operations, which convert the current cell into new cell state by the transaction rule. It will minimize the loss of data by using Fuzzy-AHP and it can lead the flexible urban growth modeling. However, AHP would have a disadvantage to repeat the procedure of the collecting intentions until it derives the weight. Also, it is necessary for the simulation of CA to repeat the operations and the test of data accuracy should be accompanied. The purpose of this study is to predict the Busan city growth model and analyze it according to the automated test method by applying CA as well as Fuzzy-AHP. This study shall improve the difficulties caused by complexity and repetitiveness in the urban grow modeling. The practical modeling could be derived from the verification, and the derived modules could be applied to the similar case studies.

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Analysis of Chromosome Composition of Gastrodia elata Blume by Fluorescent in situ Hybridization using rDNA and Telomeric Repeat Probes (rDNA와 말단소체 반복서열 탐침을 이용한 천마의 FISH 염색체 조성 분석)

  • Zhou, Hui Chao;Park, Eung Jun;Kim, Hyun Hee
    • Korean Journal of Medicinal Crop Science
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    • v.26 no.2
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    • pp.113-118
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    • 2018
  • Background: Gastrodia elata Blume is a saprophytic perennial plant in the Orchidaceae family, because of its agricultural and medicinal effectiveness, researchers focus on its genome and chemical components. However, cytogenetic information based on the chromosome structure and composition to construct chromosomal backbone for genome sequencing research and for the development and breeding of plants is very limited. Methods and Results: We determined the metaphase chromosome composition of the G. elata genome by fluorescence in situ hybridization (FISH) using 5S and 45S rDNAs and telomeric repeat probes. The nuclear genome of G. elata was organized into 2 n = 36, with relatively small ($2.71-5.50{\mu}m$)chromosomes that showed gradual decrease in size. Conglutination phenomenon was observed among the metaphase chromosomes, and it was distinguished from that in other plant metaphase chromosome spreads. One pair of signal was detected for each 5S and 45S rDNA in the pericentromeric region and interstitial region on the short arm of chromosomes 10 and 4, respectively, and telomeric DNA signals were detected in the terminal region of most chromosomes. Conclusions: To our knowledge, this is the first FISH chromosome composition result in G. elata and could be useful in more comprehensive molecular cytogenetic and genomic analyses as well as breeding programs of the medicinal plant G. elata.

Development of Chloroplast DNA-Based Simple Sequence Repeat Markers for Angelica Species Differentiation (당귀 종판별을 위한 엽록체 기반 SSR 마커 개발)

  • Park, Sang Ik;Kim, Serim;Gil, Jinsu;Lee, Yi;Kim, Ho Bang;Lee, Jung Ho;Kim, Seong Cheol;Jung, Chan Sik;Um, Yurry
    • Korean Journal of Medicinal Crop Science
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    • v.24 no.4
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    • pp.317-322
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    • 2016
  • Background: In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species. Methods and Results: A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00. Conclusions: These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.

Studies on the Incidence of Reproductive Disorder in Hanwoo (한우 암소의 번식장애에 관한 조사 연구)

  • 백광수;성환후;고응규;이명식;류일선;강희설;조원모;신기준
    • Korean Journal of Animal Reproduction
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    • v.21 no.4
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    • pp.411-421
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    • 1997
  • A survey was carried out to investigate the symptoms and occurance of reproductive disorder in Hanwoo(Korean native cattle). Data of the reproductive disorder of 561 heads from 28 farm households have been collected from Dec. '95 to Nov. '96 and analyzed calving no, nutritional body condition and housing forms. The results obtained are summarized as follows: 1. The incidence of reproductive disorder was 20.1% and the major common symptoms were repeat breeding(39.8%), anestrous(31.0%), nymphomania(10.6%) and subestrous(8.9%). 2. The incidence of reproductive disorder according to the calving no., that of heifer, calved one to two calving no. and over three calving no. were 20.6%, 13.9% and 34.5%, respectively. 3. Reproductive disorder incidence according to the nutritional body condition was 18.3%, 14.6% and 48.7% at body condition score(BCS) less than 2.0, 2.5 to 3.0 and over than 3.5, respectively. At BCS over 3.0, the symptoms of common reproductive disorder were repeat breeding(17.6%), anestrous(12.2%), nymphomania(10.8%) and subestrous(2.7%). 4. The incidence of reproductive disorder according to the housing form 15.8% and 34.6% for group feeding in open house and individual stanchion feeding in stall, respectively. In group feeding, reproductive disorder incidence of cows raised in space of more than 9.9$m^2$ per head was 14.1%, while that of cows raised less than 9.9$m^2$ per head was 18.2%. And incidence of repeat breeding, aneestrous and subestrous was more frequent in individual stanchion feeding than group feeding.

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Telomeres Distribution and Telomerase Activity During Chick Embryonic and Developmental Stages

  • Cho, E.J.;Kang, M.Y.;Jung, G.S.;Sohn, S.H.
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2003.10a
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    • pp.111-111
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    • 2003
  • Telomeres are the end of chromosomes and consist of a tandem repeat sequence of (TTAGGG)n and associated proteins. Telomerase is a ribonucleoprotein which act as a template for the synthesis of telomeric DNA. Telomeres are essential for chromosome stability and are related with cell senescence, apoptosis and cancer. Even though telomeres and telomerase have been studied extensively, very little is known about telomere dynamics in embryonic cells. This study was carried out to analyze the telomeres distribution and telomerase activity of chicken cells during embryonic and developmental stages. The target cells for analysing were sperms, ovulated ova, early embryonic cells and the cells from brain, heart, liver, kidney and germinal tissue in fetus. Telomeres distribution on target cells was analyzed by Q-FISH (Quantitation-Fluorescence in situ Hybridization) techniques using a chicken telomere repeat probe. Telomerase activity was performed by TRAP assay (Telomeric repeat Amplification Protocol) with target DNA. In results, the telomeres of chicken were found at the ends of all chromosomes. In addition, chicken had interstitial telomeres on chromosomes 1, 2 and 3. Telomerase activity was highly detectable in early embryonic cells, germinal tissues and kidney cells. Whereas telomerase activity was gradually down-regulated when the organs, including brain, heart, and liver, were developed from embryos. In the distribution of telomeric DNA on the embryonic and developmental stages, most of the cells was gradually decreased in telomere quantity during ontogenesis.

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Assessment of Genetic Relationship among Watermelon Varieties Revealed by ISSR Marker (Inter-simple sequence repeat (ISSR) marker를 이용한 수박의 품종간 유연관계 분석)

  • Kwon Yong-Sham;Lee Won-Sik;Cho Il-Ho
    • Journal of Life Science
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    • v.16 no.2 s.75
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    • pp.219-224
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    • 2006
  • Inter-simple sequence repeat (ISSR) analysis were used to assess genetic diversity among 18 genotypes of watermelon (Citrullus lanatus Thunb.) including breeding lines and commercial varieties. The 21 ISSR primers selected from 100 primers were showed the amplification of 105 reproducible fragments ranging from about 200 bp to 5000 bp. A total of 58 DNA fragments were polymorphic with an average 2.7 polymorphic bands per primer. The polymorphic primers were divided into 18 anchored primers and 3 non anchored primers. All of the anchored primers were di-nucleotide repeat motif, and was more polymorphic than non anchored primers. Eighteen watermelon genotypes were classified into two large groups. Clustering was in some accordance with the division of fruit shape into 18 watermelon. Therefore, ISSR markers may be suitable for variety discrimination and for constructing a linkage map of watermelon.

Performance Analysis of Incremental Cooperative Communication with Relay Selection Based on The Relays Arrangement (중계기 선택 기법이 적용된 증분 협력 통신의 중계기 배치에 따른 성능 분석)

  • Kim, Lyum;Kong, Hyung-Yun
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.22 no.10
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    • pp.941-950
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    • 2011
  • In this paper, we analysis the end-to-end performance of the incremental cooperative communication with relay selection. In the conventional cooperative scheme, the source(S) broadcasts the signal to the relay(R) and the destination(D) at 1st phase, and the R forwards the signal to the D at 2nd phase. Although this scheme can improve performance and provide diversity gain, it suffers from decreasing spectrum efficiency. In order to overcome this problem, the incremental cooperative model can be used. In this paper, we study two incremental cooperative method : the first uses ARQ with threshold SNR and the second uses HARQ with channel coding. we also evaluated performance of the incremental cooperative communication based on the R arrangement by using both methods.

A Cooperative ARQ Scheme for Single-hop and Multi-hop Underwater Acoustic Sensor Networks (단일-홉과 다중-홉 수중 음향 센서 네트워크에서의 효율적인 협력 재전송 기법)

  • Lee, Jae-Won;Cho, Ho-Shin
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.5B
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    • pp.539-548
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    • 2011
  • We propose an efficient cooperative ARQ (Automatic Repeat reQuest) scheme for single-hop and multi-hop underwater acoustic communications, in which cooperative nodes are used to provide more reliable alternative paths for a specific source-to-destination connection. This alternative path has higher channel quality than that of the direct source-destination path. In addition, during a packet-relay through multiple hops, the typical acknowledgement (ACK) signal is replaced with overhearing data packet returned back from the next hop. The usage of overhearing as an ACK improves the system performance. In this paper, we evaluate the proposed scheme by comparing it with a conventional S&W ARQ in terms of throughput efficiency. Computer simulation results show that the proposed cooperative retransmission scheme can significantly improve the throughput by increasing the probability of successful retransmission.

Cloning and DNA Sequencing for Unstable Minisatellites DNA Regions in E. coli. (대장균 내에서 불안정한 Minisatellite DNA 영역의 클론닝 및 DNA 염기서열 결정)

  • 임선희;김재우;김광섭;정윤희;윤세련;배호정;안태진;선우양일
    • Korean Journal of Microbiology
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    • v.40 no.2
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    • pp.65-72
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    • 2004
  • Instability of some eukaryotic sequence propagated in prokaryotic hosts is a frequently observed phenomenon. It is well documented that long inverted repeats, AT-rich sequences with structures like Z-DNA are extremely unstable in E. coli. These sequences may either be under-represented or even lost when cloned in E. coli. When we analyzed the polymorphic pattern for several tandom repeat (TR) in human SCKI gene, we found some TR regions were frequently deleted from plasmids and had difficult problem for their sequencing. These regions may result in non-clonability of the DNA sequence. Here we have cloned two difficult TR regions under low temperature and made two library for DNA sequencing using a nebulizer or sonicator. This study will help to determine the unstable genomic elements in complex mammalian genome.