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http://dx.doi.org/10.7783/KJMCS.2016.24.4.317

Development of Chloroplast DNA-Based Simple Sequence Repeat Markers for Angelica Species Differentiation  

Park, Sang Ik (Department of Industrial Plant Science and Technology, Chungbuk National University)
Kim, Serim (Department of Industrial Plant Science and Technology, Chungbuk National University)
Gil, Jinsu (Department of Industrial Plant Science and Technology, Chungbuk National University)
Lee, Yi (Department of Industrial Plant Science and Technology, Chungbuk National University)
Kim, Ho Bang (Life Sciences Research Institute, Biomedic Co. Ltd.)
Lee, Jung Ho (Green Plant Institute)
Kim, Seong Cheol (Department of Herbal Crop Research, NIHHS, RDA)
Jung, Chan Sik (Department of Herbal Crop Research, NIHHS, RDA)
Um, Yurry (Forest Medicinal Resources Research Center, National Institute of Forest Sciecne)
Publication Information
Korean Journal of Medicinal Crop Science / v.24, no.4, 2016 , pp. 317-322 More about this Journal
Abstract
Background: In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species. Methods and Results: A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00. Conclusions: These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.
Keywords
Angelica; Chloroplast DNA; Simple Sequence Repeat;
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Times Cited By KSCI : 7  (Citation Analysis)
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