• 한국기계가공학회지
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• 제1권1호
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• pp.123-132
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• 2002

In this study, the static and dynamic characteristics of endmilling process was modelled and the analytic realization of chatter mechanism was discussed. In this regard, We have discussed on the comparative assessment of recursive time series modeling algorithms that can represent the machining process and detect the abnormal machining behaviors in precision endmilling operation. In this study, simulation and experimental work were performed to show the malfunctional behaviors. For this purpose, new recursive least square method (RLSM) were adopted for the on-line system identification and monitoring of a machining process, we can apply these new algorithms in real process for detection of abnormal chatter. Also, The stability lobe of chatter was analysed by varying parameter of cutting dynamices in regenerative chatter mechanics.

• International Journal of Fluid Machinery and Systems
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• 제8권4호
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• pp.221-229
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• 2015

For efficient design process of regenerative blower, the present study provides new generalized pressure and leakage flow loss models, which can be used in the performance analysis method of regenerative blower. The present performance analysis on designed blower is made by incorporating momentum exchange theory between impellers and side channel with mean line analysis method, and its pressure loss and leakage flow models are generalized from the related fluid mechanics correlations which can be expressed in terms of blower design variables. The present performance analysis method is applied to four existing models for verifying its prediction accuracy, and the prediction and the test results agreed well within a few percentage of relative error. Furthermore, the present performance analysis method is also applied in developing a new blower used for fuel cell application, and the newly designed blower is manufactured and tested through chamber-type test facility. The performance prediction by the present method agreed well with the test result and also with the CFD simulation results. From the comparison results, the present performance analysis method is shown to be suitable for the actual design practice of regenerative blower.

• 한국항공우주학회지
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• 제37권12호
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• pp.1201-1208
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• 2009

액체로켓 연소기의 재생냉각 챔버의 제작에 사용되는 구리합금의 성형성을 돔 장출 시험과 인장시험을 수행하여 평가하였다. 성형성 평가에 사용된 시편은 열처리 유무와 재료의 방향성을 고려하여 제작하여 고온 열처리와 재료의 방향성이 성형성에 미치는 영향을 평가하였다. 시험 결과 열처리 후 구리합금 재료의 성형한계 값은 열처리 전 시편의 성형한계 값에 비하여 증가하였으나, 시편의 제작 방향에 따른 성형성의 차이는 열처리 영향에 비하여 작게 나타났다. 그리고 성형성 평가 시험방법에 따라 성형한계 값이 다르게 나타났다. 이러한 결과 들로부터 연소기 재생냉각 챔버의 내측구조물에서 실린더 형상의 구조물을 벌징 공정으로 네킹이나 파손 없이 노즐 형상으로 성공적으로 성형하기 위해서는 벌징 전 재료에 대한 고온열처리가 매우 중요함을 확인하였다. 또한 시험방법이 성형성 평가에 크게 영향을 미치는 것을 확인하였다. 본 연구를 통하여 얻은 구리합금의 성형한계도는 이 재료를 사용하여 제작하는 연소기 재생냉각 챔버 노즐의 구조설계를 위한 데이터로 활용할 예정이다.

• 한국유체기계학회 논문집
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• 제17권1호
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• pp.35-40
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• 2014

A multi-objective optimization of a regenerative fan for enhancing the aerodynamic and aeroacoustic performance was carried out using an integrated fan design system, namely, Total FAN-Regen$^{(R)}$. The Total FAN-Regen$^{(R)}$ was developed for non-specialists to carry out a series of design process, viz., computational preliminary design, three-dimensional aerodynamic and aeroacoustic analyses, and design optimization, for a regenerative fan. An aerodynamic analysis of the regenerative fan was conducted by solving three-dimensional Reynolds-averaged Navier-Stokes equations using the shear stress transport turbulence model. And, an aeroacoustic analysis of the regenerative fan was implemented in a finite/infinite element method by solving the variational formulation of Lighthill's analogy based on the results of the unsteady flow analysis. An optimum shape obtained by Total FAN-Regen$^{(R)}$ shows the enhanced efficiency and decreased sound pressure level as much as 1.5 % and 20.0 dB, respectively, compared to those of the reference design. The performance test was carried out for an optimized regenerative fan to validate the performance of the numerically predicted optimal design.

• Tissue Engineering and Regenerative Medicine
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• 제15권6호
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• pp.751-760
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• 2018

BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency. METHODS: We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM. RESULTS: We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified. CONCLUSION: Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2001년도 춘계학술대회 논문집
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• pp.971-974
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• 2001

In this study, the static and dynamic characteristics of endmilling process was modelled and the analytic realization of chatter mechanism was discussed. In this regard, We have discussed on the comparative assessment of recursive time series modeling algorithms that can represent the machining process and detect the abnormal machining behaviors in precision endmilling operation. In this study, simulation and experimental work were performed to show the malfunctional behaviors. For this purpose, new recursive(RLSM) were adopted for the on-line system identification and monitoring of a machining process, we can apply these new algorithms in real process for detection of abnormal chatter. Also, the stability lobe of chatter was analysed by varying parameter of cutting dynamices in regenerative chatter mechanics.

• 한국공작기계학회논문집
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• 제10권4호
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• pp.76-83
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• 2001

In this study, the static and dynamic characteristics of turning process was modelled and the analytic realization of regen-erative chatter mechanism was discussed. In this regard, we have discussed on the comparative assessment of recursive times series modeling algorithms that can represent the machining process and detect the abnormal machining behaviors in precision turning operation. In this study, simulation and experimental work were performed to show the malfunction behaviors. For this purpose, new Recursive Extended Instrument Variable Method(REIVM) was adopted for the on-line system identification and monitoring of a machining process. Also, we can apply REIVE algorithms in real process for the detection of chatter frequency and dynamic property and analyze the stability lobe of the system by changing a parameter of cutting dynamics in regenerative chatter mechanics, if it is stable or unstable, Also, The stability lobe of chatter was analysed.

• 한국동물학회지
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• 제28권3호
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• pp.151-165
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• 1985

솔나방의 變態에 따른 中腸上皮의 形態的 變化를 終齡幼蟲類에서 踊化直後까지 5단계로 나누어 光學 및 電子顯微鏡을 사용하여 관찰하였다. 終齡類蟲의 中腸上皮는 圓桂狀細胞, 再生細胞 및 內分泌細胞로 구성되어 있다. 終齡幼蟲의 圓桂狀細胞에서는 核으로부터 유출되는 분비물질이 核膜에 규칙적으로 배열되어 관찰되며, 정단부에서는 lysosome이 점차 증가해 간다. 또한, 遊離綠에서는 微細絨毛사이로 팽출한 細胞質突起가 나타나는데 細胞小器官은 관찰되지 않는다. 再生細胞는 分化가 활발해짐에 따라 杯胞가 있는 杯狀細胞型으로 관찰되며, 細胞質에서는 液胞가 점차 증가한다. 踊化直前에는 Ca의 주성분으로 사료되는 물질이 동심원상으로 나타나며 杯胞는 腸內腔으로 분리된다.幼蟲類의 圓桂狀細胞에서 관찰되는 lysosome의 증가, 核物質의 細胞質로 유출, 遊離綠의 細胞質突起는 幼蟲期 中腸上皮의 退化過程을 반영하고 있다고 사료되며, 再生細胞는 杯狀細胞型으로 分化되지만 杯胞가 腸內腔으로 분리되고저 踊化期의 새로운 中腸上皮를 구성한다고 믿어진다.

• Journal of Periodontal and Implant Science
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• 제52권6호
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• pp.437-454
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• 2022

Embryonic stem cells have been a popular research topic in regenerative medicine owing to their pluripotency and applicability. However, due to the difficulty in harvesting them and their low yield efficiency, advanced cell reprogramming technology has been introduced as an alternative. Dental stem cells have entered the spotlight due to their regenerative potential and their ability to be obtained from biological waste generated after dental treatment. Cell reprogramming, a process of reverting mature somatic cells into stem cells, and transdifferentiation, a direct conversion between different cell types without induction of a pluripotent state, have helped overcome the shortcomings of stem cells and raised interest in their regenerative potential. Furthermore, the potential of these cells to return to their original cell types due to their epigenetic memory has reinforced the need to control the epigenetic background for successful management of cellular differentiation. Herein, we discuss all available sources of dental stem cells, the procedures used to obtain these cells, and their ability to differentiate into the desired cells. We also introduce the concepts of cell reprogramming and transdifferentiation in terms of genetics and epigenetics, including DNA methylation, histone modification, and non-coding RNA. Finally, we discuss a novel therapeutic avenue for using dental-derived cells as stem cells, and explain cell reprogramming and transdifferentiation, which are used in regenerative medicine and tissue engineering.

• BMB Reports
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• 제47권5호
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• pp.249-255
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• 2014

Polo-like kinase-1 (Plk1) belongs to a family of serine-threonine kinases and plays a critical role in mitotic progression. Plk1 involves in the initiation of mitosis, centrosome maturation, bipolar spindle formation, and cytokinesis, well-reported as traditional functions of Plk1. In this review, we discuss the role of Plk1 during DNA damage response beyond the functions in mitotsis. When DNA is damaged in cells under various stress conditions, the checkpoint mechanism is activated to allow cells to have enough time for repair. When damage is repaired, cells progress continuously their division, which is called checkpoint recovery. If damage is too severe to repair, cells undergo apoptotic pathway. If damage is not completely repaired, cells undergo a process called checkpoint adaptation, and resume cell division cycle with damaged DNA. Plk1 targets and regulates many key factors in the process of damage response, and we deal with these subjects in this review.