• Journal of the Korean Society of Manufacturing Process Engineers
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• v.1 no.1
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• pp.123-132
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• 2002

In this study, the static and dynamic characteristics of endmilling process was modelled and the analytic realization of chatter mechanism was discussed. In this regard, We have discussed on the comparative assessment of recursive time series modeling algorithms that can represent the machining process and detect the abnormal machining behaviors in precision endmilling operation. In this study, simulation and experimental work were performed to show the malfunctional behaviors. For this purpose, new recursive least square method (RLSM) were adopted for the on-line system identification and monitoring of a machining process, we can apply these new algorithms in real process for detection of abnormal chatter. Also, The stability lobe of chatter was analysed by varying parameter of cutting dynamices in regenerative chatter mechanics.

• International Journal of Fluid Machinery and Systems
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• v.8 no.4
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• pp.221-229
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• 2015

For efficient design process of regenerative blower, the present study provides new generalized pressure and leakage flow loss models, which can be used in the performance analysis method of regenerative blower. The present performance analysis on designed blower is made by incorporating momentum exchange theory between impellers and side channel with mean line analysis method, and its pressure loss and leakage flow models are generalized from the related fluid mechanics correlations which can be expressed in terms of blower design variables. The present performance analysis method is applied to four existing models for verifying its prediction accuracy, and the prediction and the test results agreed well within a few percentage of relative error. Furthermore, the present performance analysis method is also applied in developing a new blower used for fuel cell application, and the newly designed blower is manufactured and tested through chamber-type test facility. The performance prediction by the present method agreed well with the test result and also with the CFD simulation results. From the comparison results, the present performance analysis method is shown to be suitable for the actual design practice of regenerative blower.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.37 no.12
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• pp.1201-1208
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• 2009

Dome stretching tests and tension tests were performed to evaluate the formability of a copper alloy used for manufacturing the regenerative cooling chamber. The test specimens were prepared to investigate the effect of heat treatment and direction of specimens on the formability. The test results show that forming limit values are increased by the heat treatment of the material but the variation of the forming limit values by manufacturing direction is negligible compared to the heat treatment effect, and forming limit values are also different according to the test methods. These results indicate that the high temperature heat treatment of the material before bulging is a very important process to deform the inner cylindrical structure of the regenerative cooling chamber into a nozzle shape by the bulging process without necking or fracture and the test methods also have a great effect on a evaluation of the formability. The forming limit diagram obtained in this study would be utilized to the design of regenerative cooling chamber nozzles.

• The KSFM Journal of Fluid Machinery
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• v.17 no.1
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• pp.35-40
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• 2014

A multi-objective optimization of a regenerative fan for enhancing the aerodynamic and aeroacoustic performance was carried out using an integrated fan design system, namely, Total FAN-Regen$^{(R)}$. The Total FAN-Regen$^{(R)}$ was developed for non-specialists to carry out a series of design process, viz., computational preliminary design, three-dimensional aerodynamic and aeroacoustic analyses, and design optimization, for a regenerative fan. An aerodynamic analysis of the regenerative fan was conducted by solving three-dimensional Reynolds-averaged Navier-Stokes equations using the shear stress transport turbulence model. And, an aeroacoustic analysis of the regenerative fan was implemented in a finite/infinite element method by solving the variational formulation of Lighthill's analogy based on the results of the unsteady flow analysis. An optimum shape obtained by Total FAN-Regen$^{(R)}$ shows the enhanced efficiency and decreased sound pressure level as much as 1.5 % and 20.0 dB, respectively, compared to those of the reference design. The performance test was carried out for an optimized regenerative fan to validate the performance of the numerically predicted optimal design.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.751-760
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• 2018

BACKGROUND: Bone tissue engineering based on pluripotent stem cells (PSCs) is a new approach to deal with bone defects. Protocols have been developed to generate osteoblasts from PSCs. However, the low efficiency of this process is still an important issue that needs to be resolved. Many studies have aimed to improve efficiency, but developing accurate methods to determine efficacy is also critical. Studies using pluripotency to estimate efficacy are rare. Telomerase is highly associated with pluripotency. METHODS: We have described a quantitative method to measure telomerase activity, telomeric repeat elongation assay based on quartz crystal microbalance (QCM). To investigate whether this method could be used to determine the efficiency of in vitro osteogenic differentiation based on pluripotency, we measured the pluripotency pattern of cultures through stemness gene expression, proliferation ability and telomerase activity, measured by QCM. RESULTS: We showed that the pluripotency pattern determined by QCM was similar to the patterns of proliferation ability and gene expression, which showed a slight upregulation at the late stages, within the context of the general downregulation tendency during differentiation. Additionally, a comprehensive gene expression pattern covering nearly every stage of differentiation was identified. CONCLUSION: Therefore, this assay may be powerful tools for determining the efficiency of differentiation systems based on pluripotency. In this study, we not only introduce a new method for determining efficiency based on pluripotency, but also provide more information about the characteristics of osteogenic differentiation which help facilitate future development of more efficient protocols.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.971-974
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• 2001

In this study, the static and dynamic characteristics of endmilling process was modelled and the analytic realization of chatter mechanism was discussed. In this regard, We have discussed on the comparative assessment of recursive time series modeling algorithms that can represent the machining process and detect the abnormal machining behaviors in precision endmilling operation. In this study, simulation and experimental work were performed to show the malfunctional behaviors. For this purpose, new recursive(RLSM) were adopted for the on-line system identification and monitoring of a machining process, we can apply these new algorithms in real process for detection of abnormal chatter. Also, the stability lobe of chatter was analysed by varying parameter of cutting dynamices in regenerative chatter mechanics.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.10 no.4
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• pp.76-83
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• 2001

In this study, the static and dynamic characteristics of turning process was modelled and the analytic realization of regen-erative chatter mechanism was discussed. In this regard, we have discussed on the comparative assessment of recursive times series modeling algorithms that can represent the machining process and detect the abnormal machining behaviors in precision turning operation. In this study, simulation and experimental work were performed to show the malfunction behaviors. For this purpose, new Recursive Extended Instrument Variable Method(REIVM) was adopted for the on-line system identification and monitoring of a machining process. Also, we can apply REIVE algorithms in real process for the detection of chatter frequency and dynamic property and analyze the stability lobe of the system by changing a parameter of cutting dynamics in regenerative chatter mechanics, if it is stable or unstable, Also, The stability lobe of chatter was analysed.

• The Korean Journal of Zoology
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• v.28 no.3
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• pp.151-165
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• 1985

The morphological changes of the midgut epithelium during the metamorphosis of pine caterpillar are observed with light and electron microscope, being divided into 5 stages from the 8th instar larva to just after pupation. The midgut epithelium of 8th instar larva is composed of columnar cell, goblet cell, regenerative cell, and endocrine cell. The secretorials are arranged on the nuclear membrane in the columnar cell of the midgut epithelium in the 8th instar larva, and lysosomes are augmented in the apical portion. Cytoplasmic extrusions are observed in the apical surface of columnar cell but they have no cell organells. Nucleus, mitochondria, rER, Golgi complex, and free ribsomes are observed in the regenerative cell. Regenerative cells are differentiated into the form of goblet cell, and vacuoles are gradually increased in the cytoplasm. Just pupa stage, the materials, which appears to be mainly composed of Ca, are observed in the circular form and goblet cavity of regenerative cell are detached to lumen. As a result, it reflects the process of the degeneration of the midgut epithelium that lysosomes are gradually augmented in the columnar cell, that nuclear materials are removed to cytoplasm, and that cytoplasmic extrusions are observed in the apical surface. And though regenerative cells are differentiated into the form of the goblet cell, it is believed that goblet cavity is detached from regenerative cell to the lumen and midgut epithelium of pupa stage is formed.

• Journal of Periodontal and Implant Science
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• v.52 no.6
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• pp.437-454
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• 2022

Embryonic stem cells have been a popular research topic in regenerative medicine owing to their pluripotency and applicability. However, due to the difficulty in harvesting them and their low yield efficiency, advanced cell reprogramming technology has been introduced as an alternative. Dental stem cells have entered the spotlight due to their regenerative potential and their ability to be obtained from biological waste generated after dental treatment. Cell reprogramming, a process of reverting mature somatic cells into stem cells, and transdifferentiation, a direct conversion between different cell types without induction of a pluripotent state, have helped overcome the shortcomings of stem cells and raised interest in their regenerative potential. Furthermore, the potential of these cells to return to their original cell types due to their epigenetic memory has reinforced the need to control the epigenetic background for successful management of cellular differentiation. Herein, we discuss all available sources of dental stem cells, the procedures used to obtain these cells, and their ability to differentiate into the desired cells. We also introduce the concepts of cell reprogramming and transdifferentiation in terms of genetics and epigenetics, including DNA methylation, histone modification, and non-coding RNA. Finally, we discuss a novel therapeutic avenue for using dental-derived cells as stem cells, and explain cell reprogramming and transdifferentiation, which are used in regenerative medicine and tissue engineering.

• BMB Reports
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• v.47 no.5
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• pp.249-255
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• 2014

Polo-like kinase-1 (Plk1) belongs to a family of serine-threonine kinases and plays a critical role in mitotic progression. Plk1 involves in the initiation of mitosis, centrosome maturation, bipolar spindle formation, and cytokinesis, well-reported as traditional functions of Plk1. In this review, we discuss the role of Plk1 during DNA damage response beyond the functions in mitotsis. When DNA is damaged in cells under various stress conditions, the checkpoint mechanism is activated to allow cells to have enough time for repair. When damage is repaired, cells progress continuously their division, which is called checkpoint recovery. If damage is too severe to repair, cells undergo apoptotic pathway. If damage is not completely repaired, cells undergo a process called checkpoint adaptation, and resume cell division cycle with damaged DNA. Plk1 targets and regulates many key factors in the process of damage response, and we deal with these subjects in this review.