• Title/Summary/Keyword: reflux esophagitis

Search Result 120, Processing Time 0.025 seconds

Effect of Toosendan Fructus on Chronic Acid Reflux Esophagitis Rats (천연자(川練子)가 만성 역류성 식도염 흰쥐에 미치는 효과)

  • Lee, Jin A;Shin, Mi-Rae;Choi, Jeong Won;Roh, Seong-Soo
    • The Korea Journal of Herbology
    • /
    • v.36 no.3
    • /
    • pp.1-8
    • /
    • 2021
  • Objective : Reflux esophagitis (RE), one of gastroesophageal reflux disease (GERD), is a disease that causes inflammation due to reflux of stomach contents such as stomach acid and pepsin due to the unstable gastroesophageal sphincter, and is currently increasing worldwide. The currently used treatment for reflux esophagitis has various side effects. Therefore, in this study the effect of Toosendan Fructus extract on chronic acid reflux esophagitis in rats was evaluated in order to find a new treatment material for reflux treatment. Methods : After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), Toosendan Fructus 200 mg/kg body weight/day-treated chronic acid reflux esophagitis rats (TF, n=8). After, we were taken esophageal tissue and esophageal mucosa damage was identified, and analyzed the expression of NADPH oxidase, AP-1/MAPK-related proteins, and tight junction proteins by western blot in esophageal tissue. Results : Toosendan Fructus administration significantly protected the esophageal mucosal damage of reflux esophagitis. Also, Toosendan Fructus significantly reduced the expression of NADPH oxidases (NOX2 and p22phox) and AP-1/MAPK-related proteins (c-Fos, c-Jun, p-p38, p-ERK, and p-JNK). In addition, it significantly increased the expression of tight junction proteins (Occludin, Claudin-3, and Claudin-4). Conclusions : These results suggest that Toosendan Fructus reduced damage to the esophageal mucosa by protecting the esophageal mucosa by upregulating tight junctions proteins as well as inhibiting the AP-1/MAPK pathway through reducing NADPH oxidases expression.

Protective Effect of ECQ on Rat Reflux Esophagitis Model

  • Jang, Hyeon-Soon;Han, Jeong Hoon;Jeong, Jun Yeong;Sohn, Uy Dong
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.16 no.6
    • /
    • pp.455-462
    • /
    • 2012
  • This study was designed to determine the protective effect of Rumex Aquaticus Herba extracts containing quercetin-3-${\beta}$ -D-glucuronopyranoside (ECQ) on experimental reflux esophagitis. Reflux esophagitis was induced by surgical procedure. The rats were divided into seven groups, namely normal group, control group, ECQ (1, 3, 10, 30 mg/kg) group and omeprazole (30 mg/kg) group. ECQ and omeprazole groups received intraduodenal administration. The Rats were starved for 24 hours before the experiments, but were freely allowed to drink water. ECQ group attenuated the gross esophagitis significantly compared to that treated with omeprazole in a dose-dependent manner. ECQ decreased the volume of gastric juice and increased the gastric pH, which are similar to those of omeprazole group. In addition, ECQ inhibited the acid output effectively in reflux esophagitis. Significantly increased amounts of malondialdehyde (MDA), myeloperoxidase (MPO) activity and the mucosal depletion of reduced glutathione (GSH) were observed in the reflux esophagitis. ECQ administration attenuated the decrement of the GSH levels and affected the MDA levels and MPO activity. These results suggest that the ECQ has a protective effect which may be attributed to its multiple effects including anti-secretory, anti-oxidative and anti-inflammatory actions on reflux esophagitis in rats.

Effect of a Mixture of Rhei Rhizoma and Scutellariae Radix Extract on Acute Reflux Esophagitis Rats (대황(大黃)과 황금(黃芩) 추출물 혼합물이 급성 역류성 식도염 흰쥐에 미치는 효과)

  • Lee, Jin A;Shin, Mi-Rae;Lee, Sang-Nam;Park, Soon-Ae;Park, Hae-Jin
    • The Korea Journal of Herbology
    • /
    • v.35 no.6
    • /
    • pp.43-53
    • /
    • 2020
  • Objective : Reflux esophagitis is a disease caused by reflux of stomach contents, stomach acid, and pepsin into the esophagus, and is currently increasing worldwide. This study was conducted to evaluate the effect of a mixture of Rhei Rhizoma and Scutellariae Radix (RS) extract on acute reflux esophagitis in rats. Methods : Rats were divided into five groups for examination: Normal group (Nor, n=8), water-treated acute reflux esophagitis rats (Con, n=8), tocopherol 30 mg/kg body weight/day-treated acute reflux esophagitis rats (Toco, n=8), RS 100 mg/kg body weight/day-treated acute reflux esophagitis rats (RS100, n=8), RS 200 mg/kg body weight/day-treated acute reflux esophagitis rats (RS200, n=8). All rats fasted for 18 h and then were derived by linking the metastatic junction between pylorus and forestomach and corpus. And rats were sacrificed 5 h after surgery. We analyzed the expression of NADPH, MAPK, inflammatory, anti-inflammatory, and tight junction related proteins by western blot in esophageal tissue and observed the level of reactive oxygen species (ROS), alanine aminotransferanse (ALT), and aspartate aminotransferase (AST) in serum. Results : RS administration significantly protected the esophageal mucosal damage of reflux esophagitis, and ROS, AST, and ALT levels were significantly reduced in RS administration compared to Con group. In addition, RS administration effectively suppressed MAPK and NF-κB pathways and upregulated protein expressions of tight junction protein. Conclusions : These results suggest that RS protected the esophageal mucosa by inhibiting the MAPK and NF-κB pathways and upregulating tight junctions.

A Case Study of Patients with Reflux Esophagitis Using Ortho-Cellular Nutrition Therapy (OCNT) (세포교정영양요법(OCNT)을 이용한 역류성식도염 환자 사례 연구)

  • Cheon, Neung Soo
    • CELLMED
    • /
    • v.13 no.4
    • /
    • pp.15.1-15.6
    • /
    • 2023
  • Objective: A case report on improving the symptoms of patients with reflux esophagitis by Ortho-Cellular Nutrition Therapy (OCNT). Methods: A 61-year-old Korean male who has been taking proton-pump inhibitors in the hospital for a long time due to the symptoms of reflux esophagitis. Results: The practice of Ortho-Cellular Nutrition Therapy (OCNT) restored the patient's mucosal cells of the esophageal sphincter, which led to the judgment that he was cured of reflux esophagitis. Conclusion: Ortho-Cellular Nutrition Therapy (OCNT) can be effective in relieving the symptoms of patients with reflux esophagitis.

A Comparative Study of Sepiae Os, Arcae Concha, Ostreae Concha and Esomeprazole in a Mouse Model of Reflux Esophagitis (역류성 식도염 생쥐 모델에서 해표초, 와릉자, 모려와 Esomeprazole의 치료효과에 대한 비교 연구)

  • Song, Chang-Hun;Baek, Tae-Hyun
    • The Journal of Korean Medicine
    • /
    • v.39 no.2
    • /
    • pp.92-105
    • /
    • 2018
  • Objectives: This aim of this study is to compare the reflux esophagitis improvement effects of Sepiae Os, Arcae Concha, Ostreae Concha, and Proton Pump Inhibitor(esomeprazole) through rat experiments. Methods: NO production inhibitory effect was measured by NO production amount and iNOS mRNA expression level in cell lines. iNOS, $TNF-{\alpha}$ and $p-I{\kappa}B$, and serotonin were compared using immunohistochemistry at the rat reflux esophagitis. Reflux esophagitis connection external form, lower esophageal sphincter, and gap were observed and an esophageal inflammatory indicator, IL-6 activity was also evaluated by immunohistochemistry. Results: NO production and iNOS mRNA expression was showed concentration dependent decrease in cell lines treated with Sepiae OS, Arcae Concha, and Ostreae Concha at the experiments of cell lines. In the suppression of iNOS and $p-I{\kappa}B$ at the rat reflux esophagitis, Sepiae Os treat group(SOT) and Ostreae Concha treat group(OCT) were more effective. In the increase of serotonin at the rat reflux esophagitis, ACT, MT and OCT were more effective. Damage of lower esophageal sphincter, and gap between esophageal keratin and mucosa were observed less at the SOT, ACT, OCT. In the suppression of IL-6 at the rat reflux esophagitis, SOT and OCT were more effective than GE and, SOT was more effective than MT significantly. Conclusions: The anti-inflammatory effect was the best in the SOT and lower esophageal sphincter muscle contraction was the best in the ACT at the rat reflux esophagitis. Sepiae OS was more effective than esomeprazole in the suppression of iNOS, $TNF-{\alpha}$, and IL-6.

Effect of Rhei Rhizoma and Scutellariae Radix Mixture in Chronic Acid Reflux Esophagitis Rats (만성 역류성 식도염에서 황련과 오수유 혼합물이 식도 점막에 미치는 영향)

  • Lee, Jin A;Oh, Min Hyuck;Shin, Mi-Rae;Roh, Seong-Soo;Park, Hae-Jin
    • Korean Journal of Pharmacognosy
    • /
    • v.52 no.3
    • /
    • pp.177-185
    • /
    • 2021
  • Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach contents reflux into the esophagus, causing complications such as mucosal damage. The purpose of this study was to determine the effect of Rhei Rhizoma and Scutellariae Radix mixture (RS) in chronic acid reflux esophagitis (CARE), one of the GERD. After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), tocopherol 30 mg/kg-treated chronic acid reflux esophagitis rats (Toco, n=8), Rhei Rhizoma and Scutellariae Radix mixture 100 mg/kg-treated chronic acid reflux esophagitis rats (RSL, n=8), Rhei Rhizoma and Scutellariae Radix mixture 200 mg/kg-treated chronic acid reflux esophagitis rats (RSH, n=8). Gross lesion of esophageal mucosa after RS treatment showed a superior enhancement compared with that of Control group. Additionally, RS significantly decreased the levels of MPO and MDA, effectively inhibited NADPH oxidase, and regulated the expression of the AMPK/LKB1/NF-κB pathway. Moreover, it significantly increased the expression of tight junction proteins. Taken together, RS not only alleviates inflammation of the esophageal mucosa via the AMPK/LKB1/NF-κB pathway by reducing oxidative stress, but also improves esophageal function by modulating tight junction proteins.

A Case Study of 4 Patients with Reflux Esophagitis Who Had Improved Gastroesophageal Reflux Disease after Treatment with SokPyeonAnGel (속편안겔 투여 후 위식도 역류질환 증상의 호전을 보인 역류성 식도염 병발 환자 치험 4례)

  • Kim, Min-ji;Jang, In-soo;Kang, Sei-young
    • The Journal of Internal Korean Medicine
    • /
    • v.36 no.1
    • /
    • pp.40-48
    • /
    • 2015
  • Objectives : This study is to report the effects of SokPyeonAnGel (SPAG) on patients with reflux esophagitis. Methods : Four patients with gastroesophageal reflux disease (GERD) symptoms, who had suffered from different symptoms, were diagnosed with reflux esophagitis by gastroscopy. SPAG was orally administered 30 minutes after each meal, 3 times a day. Analysis of the Gastroesophageal Reflux Disease Questionnaire (GerdQ) for GERD symptoms and quality of life was performed before and 1~3 weeks after SPAG treatment. Results and Conclusions : The rating scores decreased by 1~4 points after treatment. In particular, there was a 2-point decrease in “How often did you take additional medication for your heartburn and/or regurgitation?” except for one patient who hadn't taken additional medication. This suggests that SPAG could be used to treat symptoms of reflux esophagitis.

The Literatual Study of Yijin-tang-gamibang Effects on Reflux Esophagitis (역류성 식도염에서의 이진탕가미방(二陳湯加味方)의 효과에 대한 문헌적 고찰)

  • Kim, Keun-Hong;Lee, Jong-Ho;Cho, Chong-Kwan;Yoo, Hwa-Seung;Lee, Yeon-Weol
    • Journal of Haehwa Medicine
    • /
    • v.22 no.1
    • /
    • pp.119-128
    • /
    • 2013
  • Objectives: Yijin-tang-gamibang has been used in the Korean Medicine for treating various digestive disease. This study was aimed to investigate the effects and safety of Yijin-tang-gamibang in reflux esophagitis through the analysis of articles. Method: A total of 9 articles about Yijin-tang-gamibang and reflux esophagitis were used to develop this article. Results: According to basic research and clinic research data, it is supported that Yijin-tang-gamibang was useful prescription in reflux esophagitis. Yijin-tang-gamibang has favorable protective effects on the reflux esophagitis induced by pylorus and forestomach ligation in rats. After treatment with Yijin-tang-gamibang, patients showed improvement in all symptoms associated with reflux esophagitis and functional dyspepsia, including general condition. And Yijin-tang-gamibang did not show any toxic effect in single oral dose toxicity test. Conclusion: The results of this study suggest that Yijin-tang-gamibang showed favorable protective effects on the reflux esophagitis. However, it proved insufficient to confirm its efficacy owing to lack of clinical studies of high quality. So, we need well designed studies to verify clinical efficacy of Yijin-tang-gamibang hereafter.

Effect on rat model of reflux esophagitis treated with Charybdis japonica extract (민꽃게 추출물의 역류성 식도염 동물모델에서 유효성 평가)

  • Nam, Hyeon-Hwa;Seo, Yun-Soo;Lee, Ji Hye;Seo, Young Hye;Yang, Sungyu;Moon, Byeong Cheol;Kim, Wook Jin;Nan, Li;Choo, Byung Kil;Kim, Joong-Sun
    • The Korea Journal of Herbology
    • /
    • v.35 no.4
    • /
    • pp.17-23
    • /
    • 2020
  • Objectives : In this study, we investigated the protective effects of Charybdis japonica (C. japonica) water extract on the acute reflux esophagitis in rat models. Methods : Twenty rats were divided into four groups for examination: normal control group (n=6), the reflux esophagitis group (n=6), reflux esophagitis treated with positive control group (ranitidine 40 mg/kg, n=6), reflux esophagitis treated with C. japonica group (100 mg/kg, n=6). All rats fasted for 18 hr and then were induced with reflux esophagitis by a pylorus and forestomach ligation operation. After 4 hr, the rats were sacrificed. The proinflammatory cytokine and proteins expression measured by western bolt assay, and the histopathological analysis of the esophageal mucosa measured by hematoxylin and eosin staining. Results : C. japonica administration significantly was protecting esophageal mucosal damage upon histological analysis of reflux esophagitis in rats. The C. japonica treatment confirmed the protection of the reduction of claudin-5, an evaluation index of the damage of tight junctions in the reflux esophagitis. C. japonica was also found to inhibit the expression of proteins such as COX-2 and TNF-α in the rat esophagus. C. japonica markedly attenuated the activation of NF-κB and phosphorylation of IκBα at the same time. Conclusion : These results indicated that C. japonica suppressed the development of esophagitis through the modulation of inflammation by regulating NF-κB activation. Based on these findings, we concluded that C. japonica can prevent reflux esophagitis.

The Inhibitory Effect of Quercetin-3-O-${\beta}$-D-Glucuronopyranoside on Gastritis and Reflux Esophagitis in Rats

  • Min, Young-Sil;Lee, Se-Eun;Hong, Seung-Tae;Kim, Hyun-Sik;Choi, Byung-Chul;Sim, Sang-Soo;Whang, Wan-Kyun;Sohn, Uy-Dong
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.13 no.4
    • /
    • pp.295-300
    • /
    • 2009
  • It was evaluated the inhibitory action of quercetin-3-O-${\beta}$-D-glucuronopyranoside (QGC) on reflux esophagitis and gastritis in rats. QGC was isolated from the herba of Rumex Aquaticus. Reflux esophagitis or gastritis was induced surgically or by administering indomethacin, respectively. Oral QGC decreased ulcer index, injury area, gastric volume, and acid output and increased gastric pH as compared with quercetin. Furthermore, QGC significantly decreased gastric lesion sizes induced by exposing the gastric mucosa to indomethacin. Malondialdehyde levels were found to increase significantly after inducing reflux esophagitis, and were reduced by QGC, but not by quercetin or omeprazole. These results show that QGC can inhibit reflux esophagitis and gastritis in rats.