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http://dx.doi.org/10.22889/KJP.2021.52.3.177

Effect of Rhei Rhizoma and Scutellariae Radix Mixture in Chronic Acid Reflux Esophagitis Rats  

Lee, Jin A (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Oh, Min Hyuck (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Shin, Mi-Rae (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Roh, Seong-Soo (Department of Herbology, College of Korean Medicine, Daegu Haany University)
Park, Hae-Jin (DHU Bio Convergence Testing Center)
Publication Information
Korean Journal of Pharmacognosy / v.52, no.3, 2021 , pp. 177-185 More about this Journal
Abstract
Gastroesophageal reflux disease (GERD) is a gastrointestinal disorder in which stomach contents reflux into the esophagus, causing complications such as mucosal damage. The purpose of this study was to determine the effect of Rhei Rhizoma and Scutellariae Radix mixture (RS) in chronic acid reflux esophagitis (CARE), one of the GERD. After inducing reflux esophagitis through surgery, the group was separated and the drug was administered for 2 weeks; Normal rats (Normal, n=8), chronic acid reflux esophagitis rats (Control, n=8), tocopherol 30 mg/kg-treated chronic acid reflux esophagitis rats (Toco, n=8), Rhei Rhizoma and Scutellariae Radix mixture 100 mg/kg-treated chronic acid reflux esophagitis rats (RSL, n=8), Rhei Rhizoma and Scutellariae Radix mixture 200 mg/kg-treated chronic acid reflux esophagitis rats (RSH, n=8). Gross lesion of esophageal mucosa after RS treatment showed a superior enhancement compared with that of Control group. Additionally, RS significantly decreased the levels of MPO and MDA, effectively inhibited NADPH oxidase, and regulated the expression of the AMPK/LKB1/NF-κB pathway. Moreover, it significantly increased the expression of tight junction proteins. Taken together, RS not only alleviates inflammation of the esophageal mucosa via the AMPK/LKB1/NF-κB pathway by reducing oxidative stress, but also improves esophageal function by modulating tight junction proteins.
Keywords
Chronic reflux esophagitis; Rhei rhizoma; Scutellariae radix; Oxidative stress; Inflammation;
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1 Cho, Y. H., Kim, H. J., Kim, D. I., Jang, J. Y., Shin, Y. H., Cho, Y. G. and An, B. J. (2015) Effect of garlic (Allium sativum L.) stems on inflammatory cytokines, iNOS and COX-2 expressions in Raw 264.7 cells induced by lipopolysaccharide. Korean J. Food Preserv. 22: 613-621.   DOI
2 Shin, D. H. and Choi, Y. H. (2013) Glutamine deprivation inhibits invasion of human prostate carcinoma LnCap cells through inactivation of matrix metalloproteinases and modulation of tight junctions. J. Korean Soc. Food Sci. Nutr. 42: 1167-1174.   DOI
3 Mihara, M., Uchiyama, M. (1978) Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86: 271-278.   DOI
4 Van Dyke, T. E. and Kornman, K. S. (2008) Inflammation and factors that may regulate inflammatory response. J. Periodontol. 79: 1503-1507.   DOI
5 Germolec, D. R., Shipkowski, K. A., Frawley, R. P. and Evans, E. (2018) Markers of inflammation. Methods Mol. Biol. 1803: 57-79.   DOI
6 Nam, J. W. and Lee, Y. S. (2012) Role of NADPH oxidase in the mechanism of arachidonic acid-induced apoptosis in HepG2 human hepatoblastoma cells. Yakhak Hoeji 56: 80-85.
7 Ko, C. M., Lee, S. H. and Park, H. A. (2007) The relationship between reflux esophagitis, behavioral risk factors and Helicobactor pylori. Korean J. Health Promot. Dis. Prev. 7: 60-67.
8 Han, C. H., Lee J. S., Lim, H. H., Oh, Y. S., Yoon, Y. K., Kim, S. G., Kim, M. S., Ryoo, E. S., Youn, D. J., Jung, I. S., Ko, B. M., Yoo, C. B., Cho, Y. D., Cho, J. Y., Lee, M. S. and Shim, C. S. (2000) The significance of gastroesophageal reflux disease (GERD) symptoms in patients with reflux esophagitis. J. Neurogastroenterol Motil. 6: 196-205.
9 Lee, S. K. and Lim, S. W. (2016) The administration of Jeungmiyijin-tang to rats with induced gastro reflux esophagitis. J. Int. Korean Med. 37: 1030-1041.   DOI
10 Velagapudi, R., El-Bakoush, A., Lepiarz, I., Ogunrinade, F. and Olajide, O. A. (2017) AMPK and SIRT1 activation contribute to inhibition of neuroinflammation by thymoquinone in BV2 microglia. Mol. Cell. Biochem. 435: 149-162.   DOI
11 Kim, Y. H., Kim, S. Y. and Hwang, M. W. (2011) A case study of taeumin patient with gastro-esophageal reflux disease(GERD) who treated successfully with Yeoldahanso-tang(Reduohanshao-tang). J. S. C. M. 23: 132-138.   DOI
12 Park, C., Gweon, O. C., Choi, Y. H. and Kim, J. I. (2009) Aged black garlic inhibits cyclooxygenase-2 expression and prostaglandin E2 production by phorbol 12-myristate-13-acetate through inactivation of nuclear factor-kappaB. Cancer Prev. Res. 14: 161-170.
13 Vane, J. R., Bakhle, Y. S. and Botting, R. M. (1998) Cyclooxygenase 1 and 2. Annu. Rev. Pharmacol. Toxicol. 38: 91-120.
14 Lee, J. A, Shin, M. R., Park, H. J. and Roh, S. S. (2021) Effect of Evodiae Fructus and Arecae Semen mixture on esophageal mucosa in chronic acid reflux esophagitis. Biomed. Sci. Letters. 27: 77-87.   DOI
15 Fitzhugh, O. G. and Nelson, A. A. (1948) Liver tumors in rats fed thiourea or thioacetamide. Science 108: 626-628.   DOI
16 Kwon, O. J., Choo, B. K., Lee, J. Y., Kim, M. Y., Shin, S. H., Seo, B. I., Seo, Y. B., Rhee, M. H., Shin, M. R., Kim, G. N., Park, C. H. and Roh, S. S. (2016) Protective effect of Rhei Rhizoma on reflux esophagitis in rats via Nrf2-mediated inhibition of NF-Kb signaling pathway. BMC Complement Altern. Med. 16: 7.
17 Lee, J. A, Shin, M. R., Roh, S. S. and Park, H. J. (2021) Effects of a mixture of Citri Pericarpium and Scutellariae Radix on acute reflux esophagitis in rats. J. Nutr. Health. 54: 321-333.   DOI
18 Choi, H. C. (2012) Effects of AMP-activated protein kinase activating compounds and its mechanism. Yeungnam Univ. J. Med. 29: 77-82.   DOI
19 Chapelle, N., Ben Ghezala, I., Barkun, A. and Bardou, M. (2021) The pharmacotherapeutic management of gastroesophageal reflux disease (GERD). Expert. Opin. Pharmacother. 22: 219-227.   DOI
20 Hahm, K. B., Shin, Y. J., Won, O. H., Chung, J. B., Lee, S. I. and Park, I. S. (1995) Hemostatic and ulcer healing effects of endoscopic injection of submucotic fibrin glues: focus on scavenging reactive oxygen intermediates and ODC activities. Korean J. Gastroenterol. 27: 18-30.
21 Song, C. H. and Baek, T. H. (2018) A comparative study of Sepiae os, Arcae concha, Ostreae concha and esomeprazole in a mouse model of reflux esophagitis. J. Korean Med. 39: 92-105.   DOI
22 Park, C. S., Kim, D. H. and Lee, K. J. (2017) Evaluation of the effectiveness of alkaline mineral water on reflux esophagitis. J. Korean Soc. Water 4: 20-26.
23 Mousa, H. and Hassan, M. (2017) Gastroesophageal reflux disease. Pediatr. Clin. North Am. 64: 487-505.   DOI
24 Lim, S. C. (2010) Effects of Baikal Skullcap (Scutellaria baicalensis Georgi) extracts on the recovery of liver function in CCl4-exposed rats. Korean J. Pant Res. 23: 54-59.
25 Hwang, I.Y. and Jeong, C.S. (2013) A study on the antigastritic effects of rheum species extracts and their active components. J. Fd Hyg. Safety 28: 330-336.   DOI
26 Kim, B. H. (2011) Effects of Rhei Rhizoma on hastric ulcer in speague-dawley rats. Korean J. Physiology & Pathology 25: 71-77.
27 Jeun, D. J., Cha, Y. Y. and Lee, E. (2011) Inflammatory effect of Rheum undulatum L.. J. Oriental Rehab. Med. 21: 35-46.
28 Lee, Y. E., Park, H. J., Park, C. B. and Hwang, S. M. (2021) Anti-inflammatory activity of Scutellaria Baicalensis root extract in lipopolysaccharide-induced RAW 267.4 cells. Korean J. Food Sci. Technol. 53: 115-120.   DOI
29 Yang, S. H., Lee, H. S., Lee, E. and Lee, J. M. (2009) Immunomodulatory activity of Scutellaria Baicalensis Georgi pharmacopuncture on acute inflammatory rat model. J. Meridian & Acupoint 26: 55-68.
30 Yoon, H. J. and Park, Y. S. (2010) Effects of Scutellaria baicalensis water extract on lipid metabolism and antioxidant defense system in rats fed high fat diet. J. Korean Soc. Food Sci. Nut. 39: 219-226.   DOI
31 Lee, J. A, Shin, M. R., Lee, S. N., Park, S. A. and Park, H. J. (2020) Effect of a mixture of Rhei Rhizoma and Scutelariae Radix extract on acute reflux esophagitis rats. Kor. J. Herbol. 35: 43-53.   DOI
32 Omura, N., Kashiwagi, H., Chen, G., Suzuki, Y., Yano, F. and Aoki, T. (1999) Establishment of surgically induced chronic acid reflux esophagitis in rats. Scand. J. Gastroenterol. 34: 948-953.   DOI
33 Zhang, L., Liu, G., Han, X., Liu, J., Li, G. X., Zou, D. W. and Li, Z. S. (2015) Inhibition of p38 MAPK activation attenuates esophageal mucosal damage in a chronic model of reflux esophagitis. Neurohastroenterol. Motil. 27: 1648-1656.   DOI