• Archives of Hand and Microsurgery
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• 제23권4호
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• pp.223-229
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• 2018

목적: 무지 수근중수 관절염(thumb carpometacarpal joint arthritis)에 대한 관절성형술로 대다각골을 제거한 후 중수골을 안정시키는 여러 방법이 소개되어 있다. 현수 봉합 관절성형술은 덜 침습적이면서 술식이 쉬운 장점이 있다. 본 연구는 무지 수근중수 관절염 환자에서 이 술식을 이용하여 수술한 결과를 알아보고자 한다. 방법: 무지 수근중수 관절염으로 진단받고 현수 봉합 관절성형술을 시행한 환자 12명을 대상으로 후향적으로 연구하였다. 시각통증척도, 수술만족도, DASH (disabilities of the arm, shoulder and hand) 점수를 조사하였고, 단순방사선 검사에서 대다각골 공간 비율을 계측하였다. 결과: 평균 추시 기간은 23개월이었다. 수술 전후 통증 점수는 7.0에서 2.9로 유의하게 감소하여 호전을 보였고 (p<0.05), 수술만족도는 평균 6.9였다. 그러나 수술 전후 DASH 점수는 유의한 차이가 없었다(p=0.06). 평균 대다각골 공간 비율은 수술 전 0.45, 수술 직후 0.33, 마지막 추시 시 0.23이었다. 결론: 현수 봉합 관절성형술은 수술 결과가 만족스럽고, 다른 술식과 비교하여 중수골 침강에 의한 대다각골 공간의 감소도 큰 차이가 없어 진행된 무지 수근중수 관절염 환자에서 유용하게 사용될 수 있을 것으로 생각한다.

• Korean Journal of Radiology
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• 제20권5호
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• pp.830-843
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• 2019

Objective: To examine the potential of intravoxel incoherent motion (IVIM) and blood oxygen level-dependent (BOLD) magnetic resonance imaging for detecting renal changes after iodinated contrast-induced acute kidney injury (CI-AKI) development in a diabetic rabbit model. Materials and Methods: Sixty-two rabbits were randomized into 2 groups: diabetic rabbits with the contrast agent (DCA) and healthy rabbits with the contrast agent (NCA). In each group, 6 rabbits underwent IVIM and BOLD imaging at 1 hour, 1 day, 2 days, 3 days, and 4 days after an iohexol injection while 5 rabbits were selected to undergo blood and histological examinations at these specific time points. Iohexol was administrated at a dose of 2.5 g I/kg of body weight. Further, the apparent transverse relaxation rate (R2^*), average pure molecular diffusion coefficient (D), pseudo-diffusion coefficient (D^*), perfusion fraction (f) were calculated. Results: The D and f values of the renal cortex (CO) and outer medulla (OM) were significantly decreased compared to baseline values in the 2 groups 1 day after the iohexol injection (p < 0.05). A marked reduction in the D^* values for both the CO and OM was also observed after 1 hour in each group (p < 0.05). In the OM, a persistent elevation of the R2^* was detected for 4 days in the DCA group (p < 0.05). Histopathological changes were prominent, and the pathological features of CI-AKI aggravated in the DCA group until day 4. The D, f, and R2^* values significantly correlated with the histological damage scores, hypoxia-inducible transcription factor-1α expression scores, and serum creatinine levels. Conclusion: A combination of IVIM and BOLD imaging may serve as a noninvasive method for detecting and monitoring CI-AKI in the early stages in the diabetic kidney.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.38-38
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• 2017

Water stress obstructs rice growth mainly by oxidative damage in biological cells to cause a reduction of leaf photosynthesis and evapotranspiration processes. In this study, exogenous application of vanillic acid (VA) and p-hydroxybenzoic acid (PHBA) to improve drought tolerance of two Oryza sativa cultivars, Q2 and Q8 was tested. The drought evaluation based on leaf phenotypes to show that both Q2 and Q8 resulted in remarkable water-stress tolerance induced by leaf spraying pretreatment of mixed solution of $50{\mu}M\;VA+50{\mu}M\;PHBA$. The mixtures of $25{\mu}M\;VA+25{\mu}M\;PHBA$ and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treated on Q2 and Q8 in water deficit condition also indicated that total phenols, total flavonoids, and DPPH radical scavenging activity were significantly greater their controls. In general, the accumulation of individual phenolic acids was increased in exogenous phenolic treatments, as compared with controls. Particularly, Q2 obtained a considerable amount of endogenous PHBA after application of $50{\mu}M\;VA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ (0.18 mg/g DW, 0.71 mg/g DW, and 1.41 mg/g DW, respectively); and a negligible content of VA (0.003 mg/g DW) appeared uniquely in the treatment of $50{\mu}M\;VA$. Similarly, Q8 also absorbed a significant quantity of PHBA in $50{\mu}M\;PHBA$, $25{\mu}M\;VA+25{\mu}M\;PHBA$, and $50{\mu}M\;VA+50{\mu}M\;PHBA$ treatments (0.15 mg/g DW, 0.15 mg/g DW, and 0.22 mg/g DW, respectively). In addition, the spraying $50{\mu}M\;PHBA$ and $25{\mu}M\;VA+25{\mu}M\;PHBA$ on Q8 leaves induced similar amount of drought tolerance of Q2 and Q8 were improved, paralleled with the increased amounts of endogenous phenolics revealed that VA and PHBA played an important role to enhance drought tolerance in rice.

• International Journal of Oral Biology
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• 제36권3호
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• pp.117-122
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• 2011

Endothelial cells are a vital constituent of most mammalian organs and are required to maintain the integrity of these tissues. These cells also play a major role in angiogenesis, inflammatory reactions, and in the regulation of thrombosis. Angiogenesis facilitates pulp formation and produces the vessels which are essential for the maintenance of tooth homeostasis. These vessels can also be used in bone and tissue regeneration, and in surgical procedures to place implants or to remove cancerous tissue. Furthermore, endothelial cell regeneration is the most critical component of the tooth generation process. The aim of the present study was to stimulate endothelial regeneration at a site of acute cyclophosphamide (CP)-induced endothelial injury by treatment with human umbilical cord-derived endothelial/mesenchymal stem cells (hEPCs). We randomly assigned 16 to 20-week-old female NOD/SCID mice into three separate groups, a hEPC ($1{\times}10^5$ cells) transplanted, 300mg/kg CP treated and saline (control) group. The mice were sacrificed on days 5 and 10 and blood was collected via the abdominal aorta for analysis. The alanine transaminase (ALT), aspartate aminotransferase (AST), serum alkaline phosphatase (s-ALP), and albumin (ALB) levels were then evaluated. Tissue sections from the livers and kidneys were stained with hematoxylin and eosin (HE) for microscopic analysis and were subjected to immunohistochemistry to evaluate any changes in the endothelial layer. CP treatment caused a weight reduction after one day. The kidney/body weight ratio increased in the hEPC treated animals compared with the CP only group at 10 days. Moreover, hEPC treatment resulted in reduced s-ALP, AST, ALT levels compared with the CP only group at 10 days. The CP only animals further showed endothelial injuries at five days which were recovered by hEPC treatment at 10 days. The number of CD31-positive cells was increased by hEPC treatment at both 5 and 10 days. In conclusion, the CP-induced disruption of endothelial cells is recovered by hEPC treatment, indicating that hEPC transplantation has potential benefits in the treatment of endothelial damage.

• 생명과학회지
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• 제21권12호
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• pp.1761-1771
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• 2011

방사선 조사에 대한 방호 효과와 방사선 치료에 의한 부작용 경감 효과를 가지는 새로운 생약복합조성물(HemoHIM)의 자외선 조사에 의한 피부 면역계 방호 효과에 대해 알아보았다. IL-4와 GM-CSF로 분화시킨 수 지상세포에 자외선을 직접 조사하여 항원전달기능의 감소를 유발하는 자외선 조사 조건을 확립하고, HemoHIM을 자외선 조사 전 후에 처리하여 자외선에 의해 감소한 수지상세포의 항원전달기능이 회복되는 것을 확인하였다. 그리고 접촉성 과민반응 모델을 이용하여 피부의 Langerhans 세포의 항원전달기능의 감소를 유발하는 자외선 조사 조건을 확립하고, Langerhans 세포의 항원전달기능의 감소를 유발하고 HemoHIM을 각각 복강 또는 경구로 투여하는 방법으로 처리하였을 때, 억제된 Langerhans 세포의 항원전달 기능을 회복시키는 효과가 있다는 것을 확인하였다. 이상의 결과로 HemoHIM은 자외선 조사로 저하된 피부 면역 기능을 회복시키는 효과가 있다는 것을 증명하였고, 피부 면역 기능을 개선하는 새로운 자외선 차단제 개발을 위한 소재로 사용할 가능성을 제시하였다.

• 생물환경조절학회지
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• 제21권4호
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• pp.354-361
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• 2012

외국에서 도입한 블루베리의 내한성을 평가하고, 목재수분계측기가 월동 중 블루베리 가지의 동해피해를 간편하고 신속하게 진단하는데 활용할 수 있는지를 알아보기 위하여 수행되었다. 월동 중 블루베리의 가지의 고사율은 0~100%로 다양하게 나타나 품종별 내한성의 차이가 컸다. 블루베리 가지의 저온처리에 따른 TTC 검정에서 품종별 OD값은 $-40^{\circ}C$ < $-21^{\circ}C$ < $4^{\circ}C$ 순으로 처리온도가 낮을수록 낮게 나타났다. 블루베리 가지의 저온처리에 따른 가지절단면의 색의 검정은 가지고 사율에 의한 내한성과 다른 결과와 차이가 있었다. 목재수분계측기에 의해 측정된 살아있는 블루베리 가지의 월동 중 최저 수분함량은 약 15%였으며, 월동 중 블루베리 가지의 위치별 수분함량은 나무 아랫부분일수록 높고 가지 끝으로 갈수록 낮았으나, 봄으로 접어들면서 가지 끝의 수분함량이 점점 높아져 20~40% 범위로 측정되었다. 월동 중 동해피해를 받은 가지는 점점 건조되어 수분함량이 5% 이하로 낮아졌다. 동해를 받은 블루베리 가지의 수분함량은 14% 수준 이하일 것으로 추정되며, 목재수분계측기가 블루베리 가지의 동해피해를 현장에서 신속하게 진단하는데 활용될 수 있을 것으로 기대된다.

• 농촌계획
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• 제24권4호
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• pp.135-146
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• 2018

A Visual landscape planning and management system has been introduced and implemented by each ministry so as to solve the problems of visual landscape destruction due to recognition on the value of natural landscape of beautiful territory and various development projects. At present, this system emphasizes the importance of the visual and perceptual aspect of the landscape however, there is a lack of techniques required for comprehensively predicting, evaluating, and managing it. Furthermore, sustainable landscape management after the completion of development projects has been inadequately carried out, as the focus has been only on consultation in the planning process of the development project in institutional performance. To this end, we presented objective and standardized criteria to predict and judge the effects of development projects on landscapes before project implementation. During the implementation of the development project, the influence of the visual landscape becomes accumulated in the construction progress stage. There is a need to identify the main viewpoints and to examine the continuous changes in the landscape-influencing factors, owing to the remarkable influences on the landscape, such as the change in the topography and the change caused by the artificial structure. During the stage of managing the influence on the visual landscape after the completion of the project, the influence on landscape should be monitored by measuring the change in the continuous landscape-influencing factors and determining the extent to which the actual reduction plan has been implemented. These processes should be performed continuously to maintain the quality of the visual landscape. The change in the landscape caused by the development project is shown to cause relatively greater visual damage than other factors composing the landscape owing to the influence of the artificial factors including the structure or the building. This shows that not only detailed examination of the visual impact before the development project but also continuous management is required during and after the development project. For this purpose, we derived eight landscape-influencing factors including form/shape, line, color, texture, scale/volume, height, skyline, and landscape control point. The proposed considering to be of high utilization in that it has a clear target of the landscape influencing factors.

• 동의생리병리학회지
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• 제33권2호
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• pp.102-108
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• 2019

This study aims to evaluate the anti-inflammatory effect of Coptidis Rhizoma (CR) extract for atopic dermatitis through maintaining skin barrier and regulating Th2 cell differentiation. We divided NC/Nga mice into 3 groups as follows; atopy-like dermatitis induced group with CR treatment (CT, n=10), no treatment group(Ctrl), atopy-like dermatitis elicited group(AE). Atopy-like dermatitis was induced to NC/Nga mice by sensitizing with dermatophagoides farinae(DfE) on 7, 8, 9, 11, 12, and 13th week. After inducing atopic dermatitis, CR extract was administered 20 mg/kg daily for the experimental duration to the CT group. We measured the integrity of lipid layers in the epidermis and Th2 differentiation through immunohistochemical staining against filaggrin, loricrin, IL-4, and IL-13. We also measured the distribution of subcutaneous collagen fibers by the Masson's trichrome staining. Administration of CR significantly inhibited the reduction of lipid layers in the skin that caused atopy. The expression of IL-4, IL-13, each of which is a cytokine secreted by T helper type 2 (Th2) cells, was markedly suppressed in the CT group as compared with AE group (p<0.05). CR treatment also decreased the expression of iNOS, $p-I{\kappa}B$. Atopic dermatitis induced dermatological damage to skin, such as hyperplasia of epithelium, and capillary proliferation was significantly reduced by CR administration. CR effectively inhibited the thinning of the skin barrier and inflammatory responses in atopic dermatitis-induced mice. In particular, it showed anti-inflammatory effects by reducing the expression of IL-4 and IL-13, Th2 cell cytokines, which play a crucial role in development of atopic dermatitis. Therefore, CR can be a good candidate to ameliorate and treat atopic dermatitis.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.40-44
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• 2003

Soil-borne infectious disease including Pythium aphanidermatum and Rhizoctonia solani causes severe damage to plants, such as cucumber. This soil-borne infectious disease was not controlled effectively by chemical pesticide. Since these diseases spread through the soil, chemical agents are usually ineffective. Instead, biological control, including antagonistic microbe can be used as a preferred control method. An efficient method was developed to select an antagonistic strain to be used as a biological control agent strain. In this new method, surface tension reduction potential of an isolate was included in the ‘decision factor’ in addition to the other factors, such as growth rate, and pathogen inhibition rate. Considering these 3 decision factors by a statistical method, an isolate from soil was selected and was identified as Bacillus sp. GB16. In the pot test, this strain showed the best performance among the isolated strains. The lowest disease incidence rate and fastest seed growth was observed when Bacillus sp. GB16 was used. Therefore this strain was considered as plant growth promoting rhizobacteria (PGPR). The action of surface tension reducing component was deduced as the enhancement of wetting, spreading, and residing of antagonistic strain in the rhizosphere. This result showed that new selection method was significantly effective in selecting the best antagonistic strain for biological control of soil-borne infectious plant pathogen. The antifungal substances against P. aphanidermatum and R. solani were partially purified from the culture filtrates of Bacillus sp. GB16. In this study, lipopeptide possessing antifungal activity was isolated from Bacillus sp. GB16 cultures by various purification procedures and was identified as a surfactin-like lipopeptide based on the Fourier transform infrared spectroscopy (FT-IR), nuclear magnetic resonance (NMR), high performance liquid chromatography mass spectroscopy (HPLC-MS), and quadrupole time-of-flight (Q-TOF) ESI-MS/MS data. The lipopeptide, named GB16-BS, completely inhibited the growth of Pythium aphanidermatum, Rhizoctonia solani, Penicillium sp., and Botrytis cineria at concentrations of 10 and 50 mg/L, respectively. A novel method to prevent the foaming and to provide oxygen was developed. During the production of surface active agent, such as lipopeptide (surfactin), large amount of foam was produced by aeration. This resulted in the carryover of cells to the outside of the fermentor, which leads to the significant loss of cells. Instead of using cell-toxic antifoaming agents, low amount of hydrogen peroxide was added. Catalase produced by cells converted hydrogen peroxide into oxygen and water. Also addition of corn oil as an oxygen vector as well as antifoaming agent was attempted. In addition, Ca-stearate, a metal soap, was added to enhance the antifoam activity of com oil. These methods could prevent the foaming significantly and maintained high dissolved oxygen in spite of lower aeration and agitation. Using these methods, high cell density, could be achieved with increased lipopeptide productivity. In conclusion to produce an effective biological control agent for soil-borne infectious disease, following strategies were attempted i) effective screening of antagonist by including surface tension as an important decision factor ii) identification of antifungal compound produced from the isolated strain iii) novel oxygenation by $H_2O_2-catalase$ with vegetable oil for antifungal lipopeptide production.

• 생명과학회지
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• 제20권12호
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• pp.1777-1783
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• 2010

식물체는 대사과정의 부산물로서 또는 생물학적으로 피해를 줄 수 있는 다양한 종류의 외부 스트레스에 직면했을 활성산소(Reactive Oxygen Species, ROS)를 생산한다. 이러한 oxidative 스트레스로부터 자신들을 보호하기 위하여 식물세포들은 다양한 종류의 항산화 단백질들을 보유하고 있다. 하지만 이들의 작용기작은 여전히 자세히 밝혀지지 않았다. Peroxiredoxins (Prxs)은 식물체에 광범위하게 존재하는 thiol-을 함유한 항산화 단백질로 N-말단에 존재하는 cysteine 잔기를 이용하여 hydrogen peroxide를 환원한다. 이러한 과정에서 peroxiredoxins의 활성부위인 cysteine 잔기는 선택적으로 cysteine sulfinic acid로 산화됨으로써 peroxidase activity의 불활성화를 일으킨다. 이러한 산화과정은 비가역적으로 일어난다. 최근 발견된 진핵생물들에 잘 보존된 sulphiredoxin (Srx1)이라 불리는 단백질은 cysteine-sulphinic acid를 환원시키는 기능을 지닌다. 본 논문에서는 애기장대에 존재하는 Prxs와 Srx의 기능에 대하여 서술할 예정이다.