• Microbiology and Biotechnology Letters
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• v.38 no.3
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• pp.322-328
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• 2010

Fermented skate is a traditional Korean food popular in Southwestern area of Korea. It has a characteristic flavor and alkaline pH. In this study we tried to determine the microbial flora in fermented skate using two different approaches. In culture-independent method, we amplified V2 region of 16S rRNA gene by PCR and cloned them into pUC18 plasmid to construct 16S rDNA fragment library. BLAST searches for the sequences obtained from this library revealed that uncultured bacterium clone 054E11.b was the most dominant flora in this fermented fish. In culture-dependent method, we diluted suspension of skate and spreaded on MRS, PCA, and MacConkey plates. We identified colonies grown on those plates by using PCR amplification of V2 region of 16S rRNA and DNA sequencing. BLAST searches of those DNA sequences resulted in totally different species with the observations from the 16S rDNA library analysis. Discrepancies of results obtained from both approaches suggest that the agar plates used in culture-dependent method may be different from the real condition of fermented skate. Therefore, results from culture-independent approach using 16S rDNA fragment library analysis may reflect real microbial flora in fermented skate.

• Journal of Marine Life Science
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• v.4 no.1
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• pp.38-43
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• 2019

The deformity occurring at the early developmental stage of red spotted grouper (Epinephelus akaara) causes detrimental effects on the process of juvenile production. In this study, we have compared the expressions of several key genes (insulin like growth factor 1: IGF-1, bone morphogenic protein 4: BMP4, peroxisome proliferator-activated receptors γ: PPARγ, matrix Gla protein: MGP) for morphogenesis between normal and 2 types (cephalic and jaw) of deformed juvenile fish. Expression of these genes were investigated in the brain, liver and muscle of each group of fish (n=20) by real-time PCR. Expression of IGF-1 and BMP4 mRNA in the brain and liver showed significant difference between normal and deformed fish (p<0.05). However, no difference was observed in the expression of PPARγ and MGP mRNA between normal and deformed fish in any tissues. It seems certain that IGF-1 and BMP4 are associated with the state of deformity in juvenile red spotted grouper.

• Korean Journal of Culture and Social Issue
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• v.27 no.4
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• pp.529-559
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• 2021

This study examines how people perceive and experience busyness, which is a great social pressure in the Korean society, and The socio-psychological motives that drive people's different paces of life, such as busyness or slowness, despite being in the same busy reality, were examined. Furthermore, an in-depth study was conducted on the adaptation factors in the real society according to the speed of individual life. The analysis of this study was based on an in-depth interview data of 21 unmarried men and women in their 20s and 30s, and was conducted using a phenomenological qualitative analysis method. As a result, the perception and experience of being busy had both positive and negative parts. The components of socio-psychological motivation that affect the speed of an individual's life are 'internalization of positive values of (busy/rest)', '(future/present) centric', 'selection & concentration/Inertial busyness', 'personality characteristics' were derived. In addition, 'self-selection', 'successful time management', and 'maintenance of psychological health' were derived as common factors among adaptation factors in real society, 'a sense of achievement' and 'understanding and accepting self-characteristics' were derived as discriminatory factors. became Based on these results, the value and meaning of being busy in Korean society was discussed from a social psychology perspective, and a new perspective was presented to existing studies and discussions related to the speed of life. In addition, several follow-up studies were proposed.

• Journal of Life Science
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• v.27 no.11
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• pp.1331-1339
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• 2017

DNA barcoding is the identification of a species based on the DNA sequence of a fragment of the cytochrome C oxidase subunit I (COI) gene in the mitochondrial genome. It is widely applied to assist with the sustainable development of fishery-product resources and the protection of fish biodiversity. This study attempted to verify horse-head fish (Branchiostegus japonicus) and fake horse-head fish (Branchiostegus albus) species, which are commonly consumed in Korea. For the validation of the two species, a real-time PCR method was developed based on the species' mitochondrial DNA genome. Inter-species variations in mitochondrial DNA were observed in a bioinformatics analysis of the mitochondrial genomic DNA sequences of the two species. Some highly conserved regions and a few other regions were identified in the mitochondrial COI of the species. In order to test whether variations in the sequences were definitive, primers that targeted the varied regions of COI were designed and applied to amplify the DNA using the real-time PCR system. Threshold-cycle (Ct) range results confirmed that the Ct ranges of the real-time PCR were identical to the expected species of origin. Efficiency, specificity and cross-reactivity assays showed statistically significant differences between the average Ct of B. japonicus DNA ($21.85{\pm}3.599$) and the average Ct of B. albus DNA ($33.49{\pm}1.183$) for confirming B. japonicus. The assays also showed statistically significant differences between the average Ct of B. albus DNA ($22.49{\pm}0.908$) and the average Ct of B. japonicus DNA ($33.93{\pm}0.479$) for confirming B. albus. The methodology was validated by using ten commercial samples. The genomic DNA-based molecular technique that used the real-time PCR was a reliable method for the taxonomic classification of animal tissues.

• Toxicological Research
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• v.33 no.1
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• pp.49-54
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• 2017

Vitamin D3 is a fat-soluble secosteroid responsible for enhancing intestinal absorption of calcium, iron, and other materials. Vitamin D3 deficiency, therefore, can cause health problems such as metabolic diseases, and bone disorder. Female sex hormones including estrogen and progesterone are biosynthesized mainly in the granulosa cells of ovary. In this study, we isolated granulosa cells from porcine ovary and cultured for the experiments. In order to examine the effect of vitamin D3 on the ovarian granulosa cells, the mRNA and protein levels of genes were analyzed by real-time PCR and Western blot assay. The production of estrogen from the granulosa cells was also measured by the ELISA assay. Genes associated with follicle growth were not significantly altered by vitamin D3. However, it increases expression of genes involved in the estrogen-biosynthesis. Further, estrogen concentrations in porcine granulosa cell-cultured media increased in response to vitamin D3. These results showed that vitamin D3 is a powerful regulator of sex steroid hormone production in porcine granulosa cells, suggesting that vitamin D deficiency may result in inappropriate sexual development of industrial animals and eventually economic loss.

• Korean Journal of Oriental Medicine
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• v.16 no.2
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• pp.33-41
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• 2010

Patterns are symbolic expressions of life style or sentiment of people as a product of culture. Furthermore, it is a type of language that the shape or implication does not change much over time. Patterns shown on the Korean medical containers are related to human life and health that they are not very diverse and splendid but closed used in real life. The medical containers include the alcohol container or tea-ware in broad sense, but under this study, it would be handled only for the Korean medical containers directly used to protect human health and treatment of diseases. They would be the Medicine Mortar and Pestle that is used to grind the herbal medical ingredients, pots that hold the liquid decoction, containers to keep the medical ingredients in general conceptual use for medical purposes. The patterns inscribed are classified in animal pattern, plant pattern or alphabet pattern. Turtle, crane, ginseng, and life patterns have the contemplation to pray for long life without disease or good fortune, while plum pattern, dragon pattern and the like are used in the medical containers used mainly in royal families with the social status and dignity shown. As such, patterns have not only ornament elements but also the symbolic implication to represent long life without illness for human. It provides the basic data of medical wisdom contained in the pattern and willingness of human to protect life from illness. On the basis of such research, it would require ensuing studies to make comparison and analysis with the medical containers with the patterns in the adjacent countries in the days to come.

• Cartoon and Animation Studies
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• s.9
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• pp.250-265
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• 2005

Life drawing is one of the most important curriculum not only in 2D animation but also in 3D animation. It was secret weapon of Disney how they made great success of Snow White and Seven Dwarfs and all the fame of Disney animation. Life Drawing which has its back ground history is major curriculum of famous animation school such as CAL Arts which Disney made and Sheridan College in Canada. But in Korea, a lot of the universities and colleges which have animation department do not really seem to understand the importance of the life drawing and it's effectiveness, thus do not emphasize it as a short cut to get close to the real work field. Now that the academical society as well as industry are very well of the fact that the animation education is directly related to the animation industry, the schools should have to teach step by step and closer to the basic fundamentals. The intentions of this study is to emphasize the importance of the Life Drawing, one of the essential fundamental course, which not only help the universities and colleges that have animation course to organize their curriculum. but also to help the students who graduate the program easily find their position in the animation industry.

• Molecules and Cells
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• v.43 no.4
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• pp.373-383
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• 2020

Our previous study revealed a novel role of Fas-associated death domain-containing protein (FADD) in islet development and insulin secretion. Insulin-degrading enzyme (IDE) is a zinc metalloprotease that selectively degrades biologically important substrates associated with type 2 diabetes (T2DM). The current study was designed to investigate the effect of FADD phosphorylation on IDE. We found that the mRNA and protein levels of IDE were significantly downregulated in FADD-D mouse livers compared with control mice. Quantitative real-time polymerase chain reaction analysis showed that FADD regulates the expression of IDE at the transcriptional level without affecting the stability of the mRNA in HepG2 cells. Following treatment with cycloheximide, the IDE protein degradation rate was found to be increased in both FADD-D primary hepatocytes and FADD-knockdown HepG2 cells. Additionally, IDE expression levels were reduced in insulin-stimulated primary hepatocytes from FADD-D mice compared to those from control mice. Moreover, FADD phosphorylation promotes nuclear translocation of FoxO1, thus inhibiting the transcriptional activity of the IDE promoter. Together, these findings imply a novel role of FADD in the reduction of protein stability and expression levels of IDE.

• Journal of Agricultural Extension & Community Development
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• v.4 no.1
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• pp.339-351
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• 1997

This study intended to enhance the quality of life of rural adolescents through survey 185 young people in YeonChun County, in the course of measuring the level of life of adolescents and finding several variables related with the level and drawing some applications for enhancement. As a result of this study we had some findings 1) the most young people were belonging to the second (vital feeling value) level and third (mental value) level 2) Sex, religion, tendency oriented to other person were three related variables with the level. The more the people having other-oriented tendency the higher the level of value. Female, Christian is higher in the level. 3) Many adolescents showed little hearty satisfaction concering their own life 4) The level of act in their real life were corresponded with the level of value 5) Characteristics of high value-level adolescents against low value-level ones were that they had high level of value-recognition, less family member. The hit ratio was 83.7 percent. On the basis of this study the following suggestions for the improvement of quality of life for youth in the rural area in the future. First, we should make a great effort in value education in order to clearly get the goal of the true life. The quality improvement such as the education of mental life and religion for the young people by developing programs to continously improve the value of life. Second, the necessity of behavior guiding other oriented tendency should be emphasized. We should connect adolescents with value recognition through more interest in actions. And third, it is necessary to make a professional effort for the improvement of level of value, that is, an in depth study of the theoretical systemization.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.35 no.4
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• pp.393-400
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• 2011

In this study, a procedure for the inverse estimation of the fatigue life parameters of springs which utilize the field fatigue life test data is proposed to replace real test with the FEA on fatigue life prediction. The Bayesian approach is employed, in which the posterior distributions of the parameters are determined conditional on the accumulated life data that are routinely obtained from the regular tests. In order to obtain the accurate samples from the distributions, the Markov chain Monte Carlo (MCMC) technique is employed. The distributions of the parameters are used in the FEA for predicting the fatigue life in the form of a predictive interval. The results show that the actual fatigue life data are found well within the posterior predictive distributions.