• Tuberculosis and Respiratory Diseases
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• v.71 no.4
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• pp.249-253
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• 2011

Background: Mycobacterial infection is a problem throughout the world along with the increase of immunocompromised patients. For this reason, there have been many methods for faster and more accurate diagnosis. In this study, we evaluated several laboratory methods for mycobacterial infection. Methods: From January to December 2009, 635 specimens were cultured with mycobacteria growth indicator tube (MGIT) and Ogawa media. Polymerase chain reaction (PCR) was performed with the AdvanSure tuberculosis (TB)/non-tuberculosis mycobacterium (NTM) real-time PCR Kit (LG Life Sciences, Seoul, Korea). The 69 samples showing positive culture results were identified with the AdvanSure Mycobacteria Genotyping Chip Kit (LG Life Science, Seoul, Korea). Results: Sixty-nine (10.9%) out of 635 samples showed positive results for mycobacterial culture. Among the 635 samples, 64 were positive in MGIT, but only 42 were positive in Ogawa media. Of the 635 samples, 607 (95.6%) showed the same results between MGIT and Ogawa and the results of 579 (95.4%) were also consistent with the TB/NTM real-time PCR results. However, in the case of NTM, only one (1/24, 4.2%) was positive in PCR. In the Mycobacteria genotyping chip analysis, the most frequently identified NTM species in descending order were M. avium, M. intracellulare, M. chelonae and M. abscessus. Conclusion: Culturing with a combination of MGIT and Ogawa is recommended to increase the recovery rate of mycobacteria. Although PCR missed a reasonable number of NTM, it is faster and usually gives results that concur with those from the culture. The appropriate combination of diagnostic methods with clinical correlation are necessary.

• Journal of Digital Contents Society
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• v.17 no.4
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• pp.219-226
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• 2016

In our general life, what we recognize information with our human eyes and use it is diverse and massive. But even the current technologies improved by artificial intelligence are exorbitantly deficient comparing to human visual processing ability. Nevertheless, many researchers are trying to get information in everyday life, especially concentrate effort on recognizing information consisted of text. In the fields of recognizing text, to extract the text from the general document is used in some information processing fields, but to extract and recognize the text from real image is deficient too much yet. It is because the real images have many properties like color, size, orientation and something in common. In this paper, we applies an adaptive edge enhanced MSER(Maximally Stable Extremal Regions) to extract the text area in those diverse environments and the scene text, and show that the proposed method is a comparatively nice method with experiments.

• Korean Journal of Human Ecology
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• v.13 no.1
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• pp.111-122
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• 2004

The purpose of the study lied on supporting the reeducation program which was focused on the demand with the clients by analyzing the recognition on the real situations of the reeducation on the hair designers in the nation. As a result on the subject, the responders said they could usually learn new information through the various seminars that were held by beauty product suppliers and companies, and most of the high school or under high school level designers have participated in various seminars while the designers who graduated from a junior college or universities had experienced new trends at life time education establishments(p<0.01). A hair designer who was single and paid low salary usually depended on the course inside the shop(p<0.01), and most of the information they got was through the suppliers of the beauty products. In that case, regular sources of information, such as educational establishments, informative magazines, Internet were highly recommended. A reason for unsatisfactory reeducation was due to lack of time and a reason for not participating the seminar was also due to time shortage caused by regular working hours, which was reflecting the real situation of the hair designers. As a suggestion for the reeducation establishment, most of the responders wanted vocational schools or life-time educational center. They wanted a fixed regular period for reeducation, especially over the weekend for after working hours. It means they want long-time systematic education. Considering the results, a course which is organized for a long-time systematic purpose is suggested for the hair dressers who want to be professionals in the field with the class equipped with contents of the classes, period, and place. For the purpose to be realized, improvement of the working hours in needed and it will be an incentive to make the hair designers satisfied with their work.

• Land and Housing Review
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• v.2 no.3
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• pp.269-276
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• 2011

Recently, the chance of dew condensation in apartment buildings is increasing because of several reasons. For example, ventilation rate has been decreased because of high-insulations and airtightness for saving energy. Besides, the humidity has been made by drying washes, cooking and bathing inside of apartment buildings. However, there is lack of resonable design criteria for preventing condensation in real life and real surroundings. Therefore, this study is aimed at making a resonable design criteria of preventing condensation by measuring the indoor temperature and humidity in real life. In addition to this, it is aimed at making a resonable outdoor condition and classifying regions by using weather data. The following are the results. The interior criterion for condensation was set up $25^{\circ}C$ and a relative humidity of 55%. The outdoor criterion for condensation was set up $20^{\circ}C$, $-15^{\circ}C$, and $10^{\circ}C$ respectively for the hard frost, middle, and southern areas.

• Journal of Animal Science and Technology
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• v.55 no.6
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• pp.501-508
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• 2013

This study was conducted to determine genetic correlations among carcass traits measured by ultrasound and real carcass measurements and to estimate indirect selection responses for real carcass traits based on ultrasound measurements in Hanwoo cows. To accomplish this, 22,080 ultrasound measurement records from 17,926 cows collected from 2001 to 2012 and 11,907 carcass records obtained from fattened cattle from 2008 to 2012 were used. Genetic parameters were estimated based on eye muscle area (EMA), backfat thickness (BF) and marbling score (MS) measured by ultrasound-scanning of live cows and using the official technique on chilled bovine half-carcasses after slaughtering. Heritability and genetic correlation for carcass traits were estimated using a mixed model equation that consisted of environmental effects as fixed parameters and additive genetic effects and residual effects as random parameters, assuming that traits were different between ultrasound and carcass measurements. This statistical method was applied to the average information restricted maximum likelihood method. The heritability of EMA, BF and MS measured by ultrasound were 0.33, 0.61 and 0.46, respectively, while the heritability estimates of the corresponding traits based on carcass measurements were 0.29, 0.40 and 0.38, respectively and the genetic correlation between ultrasound and carcass traits for EMA, BF and MS were 0.41, 0.78 and 0.67, respectively. The genetic correlation between ultrasound and carcass traits was highly positive. Additionally, the selection response for marbling score was estimated to be 0.42 per generation if the cows were selected based on the ultrasound scan marbling score with an assumed selection intensity of 0.8. Overall, these results indicate that the ultrasound scan technique would be applicable to judging cow selection for genetically improved meat quality.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.500-507
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• 2016

Single-molecule real-time (SMRT) sequencing allows identification of methylated DNA bases and methylation patterns/motifs at the genome level. Using SMRT sequencing, diverse bacterial methylomes including those of Helicobacter pylori, Lactobacillus spp., and Escherichia coli have been determined, and previously unreported DNA methylation motifs have been identified. However, the methylomes of Xanthomonas species, which belong to the most important plant pathogenic bacterial genus, have not been documented. Here, we report the methylomes of Xanthomonas axonopodis pv. glycines (Xag) strain 8ra and X. campestris pv. vesicatoria (Xcv) strain 85-10. We identified $N^6$-methyladenine (6mA) and $N^4$-methylcytosine (4mC) modification in both genomes. In addition, we assigned putative DNA methylation motifs including previously unreported methylation motifs via REBASE and MotifMaker, and compared methylation patterns in both species. Although Xag and Xcv belong to the same genus, their methylation patterns were dramatically different. The number of 4mC DNA bases in Xag (66,682) was significantly higher (29 fold) than in Xcv (2,321). In contrast, the number of 6mA DNA bases (4,147) in Xag was comparable to the number in Xcv (5,491). Strikingly, there were no common or shared motifs in the 10 most frequently methylated motifs of both strains, indicating they possess unique species- or strain-specific methylation motifs. Among the 20 most frequent motifs from both strains, for 9 motifs at least 1% of the methylated bases were located in putative promoter regions. Methylome analysis by SMRT sequencing technology is the first step toward understanding the biology and functions of DNA methylation in this genus.

• Journal of The Korean Association of Information Education
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• v.24 no.5
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• pp.443-451
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• 2020

In elementary school software education, a programming process is experienced through a simple problem solving process. And even this experience emphasizes that the problem-solving process is a CS Unplugged activity. However, CS Unplugged has a disadvantage in that it only learns the principles of computing, and the learner cannot experience real problem solving. In this study, a learning program using artificial intelligence robots was developed with the goal of cultivating the ability to solve problems encountered in the real life of elementary school students. Students could solve complex problems in real life from the point of view of artificial intelligence through the developed program, and increase their interest and understanding of artificial intelligence education through robot control.

• Journal of Korean Elementary Science Education
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• v.40 no.4
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• pp.433-442
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• 2021

The purpose of this study is to develop an artificial-intelligence STEAM program for real-time interactive online class for elementary science and to analyze its effect on science academic achievement and creative problem-solving ability. The applied unit was 'Life of plant', a 4th grade science subject with high difficulty in teaching and learning mainly by memorization. The theme of the program is 'Creating a doctor of plant artificial intelligence chatbot'. The results of this study were as follows: The program developed in this study had a positive effect on elementary school students' science academic achievement and creative problem-solving ability. Therefore, the artificial intelligence STEAM program for elementary science interactive online class is effective in improving students' scientific academic achievement and creative problem-solving ability, and further research on artificial intelligence STEAM education theory, method, and practice is required.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.22 no.1
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• pp.163-170
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• 2022

This paper introduces a method of extracting facial features due to unrefined inputs in real life and improving the problem of not guaranteeing the ideal performance and speed of the object recognition model through a storage algorithm through weight summation. Many facial recognition processes ensure accuracy in ideal situations, but the problem of not being able to cope with numerous biases that can occur in real life is drawing attention, which may soon lead to serious problems in the face recognition process closely related to security. This paper presents a method of quickly and accurately recognizing faces in real time by comparing feature points extracted as input with a small number of feature points that are not overfit to multiple biases, using that various variables such as picture composition eventually take an average form.

• Journal of Life Science
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• v.24 no.2
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• pp.105-111
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• 2014

The purpose of this study was to characterize equine heat-shock protein (Hsp) genes and analyze their expression pattern in various horse tissues and blood leukocytes after exercise. In a previous study, RNA sequencing of blood and skeletal muscles of thoroughbreds before and after exercise was performed using differently expressed gene (DEG) analysis. Three Hsp genes (HspH1, Hsp90${\alpha}$ and Hsp70) were selected by DEG analysis and were found to be differentially expressed in either blood or muscle. To validate and extend previous observations on these genes, we performed RT-PCR analyses of horse tissue as well as real-time qPCR analyses of blood leukocytes after exercise. mRNA expression of these Hsp genes was found to be ubiquitous in the analyzed tissues (including thyroid, colon, skeletal muscle, cecum, kidney, spinal cord, heart, and lung). In addition, Hsp mRNA expression of these genes in extracted whole blood increased after 120 minutes of exercise compared to the baseline condition. These results are in agreement with the results of human and other experimental animals, suggesting that regulatory mechanisms that are responsible for upregulation of Hsp gene transcription may be conserved among species. Further investigations to correlate Hsp gene expression patterns with athletic performance or recovery processes after exercise are warranted.