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http://dx.doi.org/10.5352/JLS.2014.24.2.105

Identification of Equine Heat Shock Proteins Gene and Their mRNA Expression Analysis after Exercise  

Cho, Hyun-Woo (Department of Animal Science, College of Life Sciences, Pusan National University)
Park, Jeong-Woong (Department of Animal Science, College of Life Sciences, Pusan National University)
Choi, Jae-Young (Department of Animal Science, College of Life Sciences, Pusan National University)
Sivakumar, S. (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University)
Kim, Nam-Young (Subtropical Animal Experiment Station, National Institute of Animal Science, RDA)
Shin, Teak-Soon (Department of Animal Science, College of Life Sciences, Pusan National University)
Cho, Seong-Keun (Department of Animal Science, College of Life Sciences, Pusan National University)
Kim, Byeong-Woo (Department of Animal Science, College of Life Sciences, Pusan National University)
Cho, Byung-Wook (Department of Animal Science, College of Life Sciences, Pusan National University)
Publication Information
Journal of Life Science / v.24, no.2, 2014 , pp. 105-111 More about this Journal
Abstract
The purpose of this study was to characterize equine heat-shock protein (Hsp) genes and analyze their expression pattern in various horse tissues and blood leukocytes after exercise. In a previous study, RNA sequencing of blood and skeletal muscles of thoroughbreds before and after exercise was performed using differently expressed gene (DEG) analysis. Three Hsp genes (HspH1, Hsp90${\alpha}$ and Hsp70) were selected by DEG analysis and were found to be differentially expressed in either blood or muscle. To validate and extend previous observations on these genes, we performed RT-PCR analyses of horse tissue as well as real-time qPCR analyses of blood leukocytes after exercise. mRNA expression of these Hsp genes was found to be ubiquitous in the analyzed tissues (including thyroid, colon, skeletal muscle, cecum, kidney, spinal cord, heart, and lung). In addition, Hsp mRNA expression of these genes in extracted whole blood increased after 120 minutes of exercise compared to the baseline condition. These results are in agreement with the results of human and other experimental animals, suggesting that regulatory mechanisms that are responsible for upregulation of Hsp gene transcription may be conserved among species. Further investigations to correlate Hsp gene expression patterns with athletic performance or recovery processes after exercise are warranted.
Keywords
Equus caballus; exercise; heat shock protein; real-time quantitative PCR; thoroughbred;
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