• Korean Journal of Plant Resources
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• v.25 no.6
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• pp.753-761
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• 2012

We performed phylogenetic analyses of a total of 21 acessions covering 5 species in the Korean Trigonotis and one outgroup species using nuclear ribosomal ITS and chloroplast rbcL, matK, ndhF sequences. Outgroup were chosen from the closely related genus Lithospermum zollingeri. Both parsimony and Bayesian Inference methods were used to reconstruct the evolutionary history of the group. The evidence collected indicated that phylogenetic relationships among Korean Trigonotis species are unresolved based on nuclear marker (ITS), as the same as based on separated chloroplast sequences. While the phylogenetic relationships of Korean Trigonotis species almost clearly were resolved in combined chloroplast sequences. Thus, the members of Trigonotis coreana can be distinguished to the members of Trigonotis peduncularis in combined cpDNA sequences and Trigonotis nakaii was treated as a synonymed to Trigonotis radicans var. sericea. In addition, the MP and BI analysis showed Trigonotis icumae as sister of the remained Korean Trigonotis species based on combined molecular markers (BI: PP = 1).

• ALGAE
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• v.18 no.3
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• pp.199-205
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• 2003

It has been considered that genus Cosmarium including Staurastrum had the problems in grouping by morphological characters. Sequence data for the Cytochrome Oxidase subunit III (coxIII) were employed to compare with taxa of two divisions of this genus, with sections in each, for evaluating the taxonomic stability of these morphological characters. The division and section systems were not coincided with the phylogeny inferred from coxIII sequences, as the previous reports from us using nuclear rDNA ITS and chloroplast rbcL sequence comparisons in this genus. Two taxa of Staurastrum were not placed within a same clade each other, and one taxon of these was grouped in Arthrodesmus clade. Two genera, Cosmarium and Staurastrum, cannot be regarded as monophyletic from this result. Mitochondrial coxIII gene was considered as a useful phylogenetic tool to evaluate evolutionary relationships of desmids as in the case of land plants.

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.476-480
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• 2020

A marine red algal species was collected from Uljin, located on the eastern coast of Korea, during a survey of marine algal flora. This alga shares the generic features of Plocamium, and is characterized by linear, flat and thin thalli with narrow axes, branches developing in alternating pairs from margins of the axes, two to four times alternately pinnately branching, linear or occasionally adaxially curved lowermost branchlets and distinctly and compactly clustered stichidia. In a phylogenetic tree based on rbcL sequences, the Korean alga nests in the same clade as P. serrulatum. The genetic distance between both sequences within the clade was calculated as 0.0-0.2%. Based on the morphological and molecular data, this Korean species is identified as P. serrulatum described originally from Taiwan. This is the first record of P. serrulatum in Korean marine algal flora.

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.481-485
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• 2020

A marine red algal species was collected from Honghyeon-ri, Namhae located on the southern coast of Korea during a survey of marine algal flora. This alga shares the generic features of Chondrus, and is characterized by short and thin, canaliculated and crisped fronds as C. retortus. Tetrasporangia are developed from shallow medullary cells on both sides of branches. In a phylogenetic tree based on rbcL sequences, the Korean alga nests in the same clade as C. retortus originally described from Japan. The genetic distance between both sequences within the clade was calculated as 0.0-0.1%. Based on the morphological and molecular data, the alga is identified as Chondrus retortus. This is the first record of C. retortus in Korean marine algal flora.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.314-318
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• 2018

A marine algal species was collected from Sacheonjin, Gangneung located on the eastern coast of Korea during a survey of marine algal flora. This alga shares the generic features of Membranoptera belonging to the subfamily Delesserioideae and is characterized by the presence of combined features of membranous, monostromatic thalli attached by a solid discoid holdfast, blades with a conspicuous terete stipe-like midrib and microscopic lateral veins, entire margins, irregularly alternate to dichotomous branching, and obtuse apices growing apically. In a phylogenetic tree based on rbcL sequences, the Korean alga nests in the same clade with M. alata from the eastern North Atlantic. The genetic distance between both the sequences within the clade was calculated as 0.0%. Based on the morphological and molecular analyses, this Korean species is identified as the generic type, M. alata. This is the first record in the list of Korean marine algal flora.

• Korean Journal of Environmental Biology
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• v.38 no.4
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• pp.611-615
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• 2020

A marine red algal species was collected from Pyengdae-ri, Gujwa in Jeju, Korea during a survey of marine algal flora. This alga shares the generic features of Callophyllis(Gigartinales, Kallymeniaceae), and is characterized by erect and membranous thalli, branches dichotomously or trichotomoulsy divided, margin somewhat undulate or entire and tetrasporangia scattered irregularly in the cortex. In a phylogenetic tree based on rbc L sequences, the Korean alga nests in the same clade as C. mageshimensis originally described from Japan. The genetic distance between both sequences within the clade was 0.0-0.1%. Based on the morphological and molecular data, the alga was identified as Callophyllis mageshimensis. This is the first record of C. mageshimensis in Korean marine algal flora.

• ALGAE
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• v.37 no.4
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• pp.249-264
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• 2022

Two genera of the Rhodymeniales, Halopeltis and Leptofauchea, are here reported for the first time from the Hawaiian Islands and represent the deepest records for both genera. Molecular phylogenetic analyses of cytochrome oxidase subunit I (COI), rbcL, and large subunit ribosomal DNA (LSU) sequences for Hawaiian specimens of Leptofauchea revealed one well-supported clade of Hawaiian specimens and three additional lineages. One of these clades is described here as Leptofauchea huawelau sp. nov., and is thus far known only from mesophotic depths at Penguin Bank in the Main Hawaiian Islands. L. huawelau sp. nov. is up to 21 cm, and is the largest known species. An additional lineage identified in the LSU and rbcL analyses corresponds to the recently described L. lucida from Western Australia, and is a new record for Hawai'i. Hawaiian Halopeltis formed a well-supported clade along with H. adnata from Korea, the recently described H. tanakae from mesophotic depths in Japan, and H. willisii from North Carolina, and is here described as Halopeltis nuahilihilia sp. nov. H. nuahilihilia sp. nov. has a distinctive morphology of narrow vegetative axes that harbor constrictions along their length. The current distribution of H. nuahilihilia includes mesophotic depths around W. Maui, W. Moloka'i, and the island of Hawai'i in the Main Hawaiian Islands. Few reproductive characters were observed because of the small number of specimens available; however, both species are distinct based on phylogeny and morphology. These descriptions further emphasize the Hawaiian mesophotic zone as a location harboring many undescribed species of marine macroalgae.

• Korean Journal of Plant Taxonomy
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• v.39 no.3
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• pp.135-142
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• 2009

Phylogeny of the family Ophioglossaceae and a phylogenetic position of Mankyua were estimated through analyses of chloroplast rbcL gene sequences and spore morphology. Sequence analysis of the rbcL gene clearly indicated that there are two major lineages in the family Ophioglossaceae: Botrychioid lineage and Ophioglossoid lineage. The Botrichioid lineage is composed of three distinct clades: Botrychium, Helminthostachys and Mankyua, where Helminthostachys and Mankyua were placed as sister groups to the Botrychium. Within the genus Botrychium, subgenera Septridium and Botrychium were monophyletic, while taxa of subgen. Botrypus branched as sister of the two, successively, thus making a non-monophyletic group. Ophioglossum formed the Ophioglossoied lineage, where the subgen. Ophioglossum is monophyletic, while subgen. Cheiroglossa and Ophoderma formed a sister relationship with subgen. Ophioglossum. In terms of external morphology and spores, Mankyua is most similar to Helminthostachys, however, patristic distance in the cladogram and trophophore characteristics of the two genera are distinct. Therefore, Mankyua is a well defined genus within the family in terms of morphology as well as molecular phylogeny which places it in basal position of the Botrychioid lineage on the gene tree.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.7
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• pp.967-972
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• 2018

The genus Chattonella belonging to the class raphidophyceae, is a harmful algal bloom species. Recently, its occurrence has been increasing and expanding along the Korean coast. Species identification of the genus Chattonella only by morphological observation is difficult due to the lack of rigid cell walls. In this study, the morphological characteristics and genetic affinity of Chattonella sp. isolated from Deungnyang Bay in 2017 were examined. We carried out single-cell isolation from field samples then sequenced three different areas using the single-cell PCR method: 1) parts of ribosomal operon, the large subunit (LSU) of the rDNA, 2) the chloroplast-encoded subunit psaA of Photosystem I, and 3) rbcL encoding the large subunit of the Rubisco gene. The cells were morphologically very similar to the general genus Chattonella ($74.0{\pm}10.1{\mu}m$ in length, $33.1{\pm}3.6{\mu}m$ in width). The three partial gene sequences were insufficient to justify distinction at the species rank. However, they clustered at 99-100 % sequence similarity with C. marina, C. marina var. antiqua and C. marina var. ovata.

• Korean Journal of Plant Taxonomy
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• v.48 no.3
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• pp.206-217
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• 2018

The nucleotide sequences of the chloroplast rbcL, matK, and psbA-trnH and nuclear internal transcribed spacer (ITS) regions were determined from all species of Viburnum in Korea with multiple accessions to reconstruct the phylogeny and to evaluate the utility of the DNA sequences as DNA barcodes. The results of phylogenetic analyses of the cpDNA and ITS data are consistent with the findings of previous studies of Viburnum. Four morphologically closely related species, V. dilatatum, V. erosum, V. japonicum, and V. wrightii, were included in a strongly supported sister clade of V. koreanum and V. opulus. Viburnum odoratissimum is suggested to be sister to the V. dilatatum/V. koreanum clade in the cpDNA data, while V. odoratissimum is a sister to V. furcatum in the ITS data. Viburnum burejaeticum and V. carlesii are strongly supported as monophyletic. Our analyses of DNA barcode regions from multiple accessions of the species of Viburnum in Korea confirm that six out of ten species in Korea can be discriminated at the species level. The V. dilatatum complex can be separated from the remaining species according to molecular data, but the resolution power to differentiate a species within the complex is weak. This study suggests that regional DNA barcodes are useful for molecular species identification in the case of Viburnum when flowering or fruiting materials are not available.