• 한국축산식품학회지
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• 제37권6호
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• pp.799-803
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• 2017

In the present study, centrifugation and filtration pretreatments were evaluated to decrease sample preparation time and to improve the sensitivity and specificity of multiplex polymerase chain reaction (PCR) for the detection of low levels of pathogenic Escherichia coli in various foods. Pathogenic E. coli (E. coli NCCP11142, E. coli NCCP14037, E. coli NCCP 14038, E. coli NCCP14039, and E. coli NCCP15661) was inoculated into pork, beef, and baby leafy vegetables at 1, 2, and 3 Log CFU/g. The samples were shaken 30 times (control), then centrifuged or filtered. DNA extracts from the samples were subjected to PCR using the $Powerchek^{TM}$ Diarrheal E. coli 8-plex Detection Kit. In the pork samples, no E. coli was detected in the control samples, while E. coli were detected in 100% of 3-Log CFU/g inoculated and centrifuged samples, and in 100% of 2 and 3-Log CFU/g inoculated, and filtered samples. In the beef samples, all control samples appeared to be E. coli-negative, while E. coli was detected in 50-75% of centrifuged samples, regardless of inoculated level, and in 100% of 2 and 3-Log CFU/g inoculated, and filtered samples. In baby leafy vegetables, E. coli were not detected in 25-50% of the control samples, while E. coli were detected in 0-25% of the centrifuged samples, and 75-100% of the filtered samples, depending on the inoculum amount. In conclusion, filtration pretreatment can be used to minimize sample preparation time, and improve the sensitivity and specificity of rapid detection of pathogenic E. coli in various foods.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.277.1-277.1
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• 2013

Molecular diagnostics consists of three processes, which are a sample pretreatment, a nucleic acid amplification, and an amplicon detection. Among three components, sample pretreatment is an important process in that it can increase the limit of detection by purifying nucleic acid in biological sample from contaminants that may interfere with the downstream genetic analysis such as nucleic acid amplification and detection. To achieve point-of-care virus detection system, the sample pretreatment process needs to be simple, rapid, and automatic. However, the commercial RNA extraction kits such as Rneasy (Qiagen) or MagnaPure (Roche) kit are highly labor-intensive and time-consuming due to numerous manual steps, and so it is not adequate for the on-site sample preparation. Herein, we have developed a rotary microfluidic system to extract and purify the RNA without necessity of external mechanical syringe pumps to allow flow control using microfluidic technology. We designed three reservoirs for sample, washing buffer, and elution buffer which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense a RNA sample solution, a washing buffer, and an elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted in the downstream. Such a novel rotary sample preparation system eliminates some complicated hardwares and human intervention providing the opportunity to construct a fully integrated genetic analysis microsystem.

• Journal of Microbiology and Biotechnology
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• 제29권4호
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• pp.548-557
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• 2019

Staphylococcus species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of Staphylococcus species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of Staphylococcus reference strains (n = 19) and isolates (n = 96) from various foods with consideration for the impact of sample preparation methods and incubation period. Additionally, the spectra of isolated Staphylococcus strains were analyzed using principal component analysis (PCA) and a main spectra profile (MSP)-based dendrogram. MALDI-TOF MS accurately identified Staphylococcus reference strains and isolated strains: the highest performance was by the EX method (83.3~89.5% accuracy) at species level identification (EDT, 70.3~78.9% accuracy; DT, less than 46.3~63.2% accuracy) of 24-h cultured colonies. Identification results at the genus level were 100% accurate at EDT, EX sample preparation and 24-h incubation time. On the other hand, the DT method showed relatively low identification accuracy in all extraction methods and incubation times. The analyzed spectra and MSP-based dendrogram showed that the isolated Staphylococcus strains were characterized at the species level. The performance analysis of MALDI-TOF MS shows the method has the potential ability to discriminate between Staphylococcus species from foods in Korea. This study provides valuable information that MALDI-TOF MS can be applied to monitor microbial populations and pathogenic bacteria in the food industry thereby contributing to food safety.

• 한국콘텐츠학회논문지
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• 제11권11호
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• pp.296-312
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• 2011

급속한 저출산과 고령화 추세를 보이고 있는 우리나라는 IMF 외환위기 이후 저성장과 저금리가 장기적으로 지속되면서 경제적으로 어려움을 겪고 있다. 이러한 여건에서 현재 중년층은 다가올 노년기에 대비하기 위해 신체적, 심리적, 사회적, 경제적 은퇴준비를 체계적으로 해야 하는 것이 매우 중요한 과업이다. 따라서 본 연구는 중년층의 직업특성과 활동특성이 신체적, 심리적, 사회적, 경제적 은퇴준비 영역에 미치는 영향을 파악하고자 한다. 수도권과 충청권 지역의 회사원, 공무원, 자영업, 전문직에 종사하는 30~50대 344명을 대상으로 구조화된 설문지를 이용하여 자료를 수집하였으며 분석한 결과를 정리하면 다음과 같다. 첫째, 연령이 높고 건강이 좋을수록 직장에서의 역할이 안정적이고 여가활동을 많이 할수록 신체적 준비를 더 많이 하고 있었다. 둘째, 남성에 비해 여성이 직장동료와 좋은 관계를 유지할수록 직업에 대한 태도가 긍정적이고 여가활동을 중요시할수록 심리적 준비를 더 많이 하는 것으로 나타났다. 셋째, 자신의 직장이 안정적이라고 인식하며 사회활동과 여가활동을 많이 할수록 사회적 준비를 더 많이 하는 것으로 나타났다. 넷째, 경제적으로 여유가 있을수록 경제적 준비 정도가 높았지만 직업특성과 활동특성은 경제적 준비에 영향을 미치지 않는 것으로 나타났다. 이러한 분석결과는 최근 사회적 이슈가 되고 있는 은퇴준비가 직업 특성과 활동특성에 의해 영향을 받고 있음을 보여주며, 향후 중년층의 은퇴준비를 위한 구체적인 정책과 프로그램이 다양하게 준비되어야 함을 의미한다.

• Strategy21
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• 통권42호
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• pp.83-113
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• 2017

After defeat in World War II, Japan's Peace Constitution committed the country to forego the acquisition of offensive military capabilities. However, in the midst of the post-cold war period, Japan began to change its security posture in line with the so-called 'normal state theory', which called for a more robust defense posture and expanded security activities. The second Abe administration promoted these security policies by issuing a National Security Strategy as well as a new National Defense Program Outline(NDPO) in 2013 and by establishing new security institutions such as the National Security Council. The Abe administration also adopted the new concept of a 'Unified Mobile Defense Force' in the 2013 which replaced the 'Dynamic Defense Force' as a new criteria for the Self-Defense Force's acquisition of military capabilities. In this new concept of military capabilities, the Ground Self-Defense Force is planning to replace existing divisions with mobile divisions and to form 'Amphibious Rapid Deployment Bridge' for the first time in 2018, which has long been taboo in Japan. Japan has experience a Marine Corps in the past. Likewise, an offensive changes in the military strategy can change the spectrum of strategy and 'Amphibious Rapid Deployment Bridge' plays a big role in this. Furthermore, Japan is increasing the Coast Guard's budget and capabilities in preparation for contingencies around the Senkaku islands (called the Diaoyu in Chinese). The South Korea navy should utilize Japan's changing security posture to deter immediate threat such as North Korea's military provocations and potential enemy threat such as China, Japan, Russia.

• 한국전기전자재료학회논문지
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• 제25권7호
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• pp.543-551
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• 2012

In this study, transparent conducting Al-doped Zinc Oxide (AZO) films with a thickness of 150 nm were prepared on corning glass substrate by the RF magnetron sputtering with using a Al-doped zinc oxide (AZO), ($Al_2O_3$: 2 wt%) target at room temperature. This study investigated the effect of rapid thermal annealing temperature and oxygen ambient on structural, electrical and optical properties of Al-doped zinc oxide (AZO) thin films. The films were annealed at temperatures ranging from 400 to $700^{\circ}C$ by using Rapid thermal equipment in oxygen ambient. The effect of RTA treatment on the structural properties were studied by x-ray diffraction and atomic force microscopy. It is observed that the Al-doped zinc oxide (AZO) thin film annealed at $500^{\circ}C$ at 5 minute oxygen ambient gas reveals the strongest XRD emission intensity and narrowest full width at half maximum among the temperature studied. The enhanced UV emission from the film annealed at $500^{\circ}C$ at 5 minute oxygen ambient gas is attributed to the improved crystalline quality of Al-doped zinc oxide (AZO) thin film due to the effective relaxation of residual compressive stress and achieving maximum grain size.

• 한국수산과학회지
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• 제23권2호
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• pp.167-177
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• 1990

매쇄 정어리 육에 정어리 자가 소화액 및 정어리 코오지를 첨가하여 발효 숙성시키므로서 질소 함량이 높고 풍미가 양호한 숙성 정어리 제조조건을 검토하였다. 정어리 자가 소화액의 제조조건은 마쇄한 정어리육에 $80\%$ 가수한 후 $55^{\circ}C$에서 180 r.p.m.으로 교반하면서 6시간 자가소화 시키는 것이 적합하였다. 속성 정어리 액젓 제조를 위한 식염 농도 및 발효 온도는 각각 $15\%$ 및 $40^{\circ}C$가 적당하였으며 마쇄정어리 육에 자가 소화액 및 정어리 기질 코오지를 각각 $10\%$ 첨가하고 식염, 밀가루, 정어리 자가 소화액을 각각 $15\%$, $10\%$ 및 $150\%$ 비율이 되도록 혼합한 후 $40^{\circ}C$에서 15일간 발효시키므로서 향미가 양호하고 질소함량이 높은 속성 정어리 액젓을 제조할 수 있었다.

• 한국식품과학회지
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• 제36권2호
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• pp.189-195
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• 2004

본 연구에서는 조제분유 중에 영양강화를 위해 첨가하는 비타민 A와 E의 함량측정을 위해 각기 다른 실험방법을 사용하지 않고 2가지 비타민을 동시에 분석하는 신속분석법을 위와 같이 수행하였다. 비타민 A와 E를 유기용매로 동시에 신속하게 추출하고 역상컬럼과 PDA-HPLC를 이용하여 각각의 성분으로 모두 분리한 후 동시에 검출하는 방법을 사용하였다. 국제표준인증물질 및 조제분유를 시료로 사용하여 본 연구의 실험방법에 의해 측정된 값을 식품공전방법 및 AOAC방법에 의한 측정값과 비교한 결과 유사한 측정결과를 얻을 수 있었다. 또한 본 연구의 실험방법에 의하여 수행한 국제인증표준물질중의 비타민 A와 E의 함량측정 결과는 인증된 표준값내의 결과를 보여주었다. 따라서, 비타민 A또는 E를 강화한 분유, 이유식 등의 분말 유제품 중에서 비타민 A와 E의 함량을 측정 하고자 할 때 한정된 장비와 인력으로 각각의 2가지 실험방법을 수행하기가 어렵거나 시간단축이 필요한 경우, 본 연구에서 수행한 실험방법과 같이 시료 전처리를 간단하고 신속하게 수행할 뿐만 아니라 역상컬럼과 PDA-HPLC에 의해 비타민 A와 E를 동시에 분석함으로써 보다 효율적인 분석을 진행 할 수 있을 것으로 사료된다.

• Journal of Biosystems Engineering
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• 제37권6호
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• pp.420-428
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• 2012

Purpose: Porcine proliferative enteropathy(PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE in a pig farm in earlier stage, a rapid diagnosing test equipment is needed for farmers. To test the equipment appropriately, we prepare the samples and antibodies for rapid detecting the Lawsonia intracellularis in pig feces. Methods : To prepare the PPE infected samples, we sampled PPE suspected pig feces in a pig farm. To manufacture a anti-Lawsonia intracellularis antibody for capturing the Lawsonia intracellularis, the rabbit-anti LsaA synthetic peptide polyclonal antibody was inoculated to rabbits. To select the couple of antibodies which is most well sandwiched with the bacteria, ELISA test was done with PPE infected ileum samples. Finally, to verify the PPE infected feces which would be used to test the rapid kit, PCR test was done on the sampled PPE suspected feces Results : The rabbit-anti LsaA synthetic peptide polyclonal antibody is developed, and is verified to capture the bacterial well through the fluorescence antibody test. Also, we found that the monoclonal antibody and the polyclonal antibody could be used as couples for sandwiching the bacteria. Finally, through the PCR test for samples of pig feces, we could prepare the 150 PPE positive samples and 50 PPE negative samples. Conclusions : The manufactured polyclonal antibody and the imported monoclonal antibody could be used to capture the bacteria using the sandwich techniques. Also, the prepared PPE infected negative and positive samples could be used to test the performance of the rapid kit to capture the bacterium Lawsonia intracellularis.

• 한국콘텐츠학회논문지
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• 제16권2호
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• pp.467-479
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• 2016

본 연구의 목적은 급격한 산업화와 도시화로 인한 핵가족화가 가족 및 개인의 책임과 의무로 여겨져 왔던 부모부양의식에 커다란 변화를 가져온 현실에 있어서, 시대 상황적으로 여러 가지 상대적으로 취약한 베이비부머 세대 스스로의 노후준비가 부모 부양의식에 미치는 영향이 어떠한지 살펴보고자 한다. 연구결과 종교, 주거상태, 월평균소득 등이 부모부양의식에 미치는 유의미한 결과를 보였으며, 경제생활관련 노후준비수준, 건강생활관련 노후준비수준, 여가생활관련 노후준비수준, 가족생활관련 노후준비수준, 주거생활 관련 노후준비수준 그리고 노후준비수준 총합 모두에서 부모부양의식과 관련하여 유의성을 나타내었다. 특히 주거생활관련 노후준비수준과 여가생활관련 노후준비수준이 부모부양의식에 미치는 영향에 있어서 유의확률이 .001(*p<0.05) 가장 유의미하게 나타났고, 이어서 경제생활관련 노후준비수준이 .005(*p<0.05)의 유의확률을 나타냈다. 결과적으로 본인들의 노후 준비수준이 높을수록 상대적으로 그들의 부모부양의식이 높음을 알 수 있었다. 따라서 이러한 연구결과를 근거로 정책적 제언을 하면, 첫 번째, 그들의 주거생활과 관련한 안정적 지원정책이 차별화하여 지원되어야 할 것이며, 두 번째로 봉사활동 등 사회활동을 통한 여가활동을 위한 사회적 프로그램의 지원이 필요하며, 아울러 아직 젊은 그들에게 경제활동을 통한 경제생활 불안감을 해소 할 수 있도록 국가의 정책적인 배려가 있어야 할 것이다.