• Journal of Korean Society of Environmental Engineers
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• v.31 no.2
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• pp.96-101
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• 2009

In this study, the effects of three different biological activated carbon (BAC) materials (each coal, coconut and wood based activated carbons), empty bed contact time (EBCT) and water temperature on the removal of sulfonamide 5 species in BAC filters were investigated. Experiments were conducted at three water temperatures (5, 15 and $25^{\circ}C$) and four EBCTs (5, 10, 15 and 20 min). The results indicated that coal based BAC retained more attached bacterial biomass on the surface of the activated carbon than the other BACs, increasing EBCT or increasing water temperature increased the sulfonamide 5 species removal in BAC columns. In the coal-based BAC columns, sulfachloropyridazine (SCP), sulfamethazine (SMT) and sulfathiazole (STZ) removal efficiencies were 30~80% and sulfadimethoxine (SDM), sulfamethoxazole (SMX) removal efficiencies were 18~70% for 5~20 min EBCT at $25^{\circ}C$. The kinetic analysis suggested a first-order reaction model for sulfonamide 5 species removal at various water temperatures (5~$25^{\circ}C$). The pseudo-first-order reaction rate constants and half-lives were also calculated for sulfonamide 5 species removal at 5~$25^{\circ}C$. The reaction rate and half-lives of sulfonamide 5 species ranging from 0.0094~0.0718 $min^{-1}$ and 9.7 to 73.7 min various water temperaturs and EBCTs in this study could be used to assist water utilities in designing and operating BAC filters for sulfonamide antibiotic compounds removal.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.32 no.2
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• pp.121-126
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• 1999

In order to degrade chitin by enzymatic hydrolysis, it is required from screening highly active deacetylase. To this end, we examined three fungal strains and it turned out that Mucor rouxii produced highly active deacetylase, this enzyme exhibited the highest enzymatic activity against colloidal chitin. The conditions for growing Mucor rouxii are as follows; the effective carbon source, nitrogen source, adequate initial pH, temperature and incubation time were $2\%$ glucose, $1.33\%$ yeast extract, $0.66\%$ pepton, 4.5, $25{\pm}2^{\circ}C$ and 48hr, respectively. The optimum pH and temperature for purified enzyme activity were 5.5 and $40^{\circ}C$, respectively. The purified enzyme was stable at pH ranging from 4.5 to 5.5. However, the enzyme activity was decreased to less than $50\%$ at pH blow 45 and above 7.5. At temperatures above $50^{\circ}C$, the enzyme activity was decreased remarkably. The enzyme was inhibited by LiC1, $HgCl_2$, and $BaCl_2$, but stimulated by $CaCl_2$ and $ZnC1_2$, The activity of purified enzyme was increased by L-cysteine and 2-mercaptoethanol, while decreased by O-phenanthroline, p-CMB, EDTA, and iodoacetate. The $K_m$ and the $V_{max}$ value of purified enzyme were $1.2\%$ and 59.5 U/mg, respectively. The deacetylation activity of purified enzyme was not detected at optimal reaction condition when chitin particle suspension was used.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.4 no.3_4
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• pp.113-120
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• 1971

In 1971, about 150,000 fingerlings of grass and silver carps were produced and distributed from the Fishculture Laboratory of Pusan fisheries College. The adults Were those transplanted from Japan in 1963. Findings during the process of this production are summarized as following : 1. Brood fish must be handled with a great care during the catching, hormone injection and maturity inspection so as the fish are not injured, otherwise, the eggs would not mature perfectly. Scale fall also affects significantly. 2. The amount of pituitary to be injected is preferable to be 2 to 3 times or more in donor's body weight to obtain good results. 3. The eggs should be spawned or stripped and inseminated as soon as mature. If the spawning is delayed the eggs become overmature and the hatching rate decreases significantly. 4. The water once used for the incubation of eggs should not be reused. The eggs under hatching process were melted away when water once used for incubation was reused. 5. A great care must be paid to keep water in quality when the net cage culture system is employed for raising fry or early fingerlings of Chinese carps. The best method to keep water in quality is to supply water in the cage continuously through a pipe or hose. 6. Heavy outbreaks of Spirogyra occured when clear well water was supplied into the cage, and a great number of fry or fingerlings were trapped or entangled resulting in a significant decrease of fish under rearing. It was prevented when moderately bloom-ed pond water was supplied into the cage. 7. Silver carp fry are usually captured and transported in the cool season because they can not be handled in the warm season owing to a high mortality, but cage cultured small size fingerlings ranging from 1.5 to 2 cm in total length are easily handled and transported even in the hot summer season, thus it enables the fish farmers to start growing them one season earlier.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.2
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• pp.59-67
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• 1983

An experiment on the rearing of tilapia stocked in closed recirculating tanks eliminating biological filter beds was carried out at the Fish Culture Experiment Station of the National Fisheries University of Pusan, from May 18 through October 21, 1982, and the growth rates, feed conversion, water quality, spawning prevention and space utilization efficiency were discussed. Finally discussed is the feasibility on the establishment of commercial production units. On the water quality, the water temperature ranged from $22.8^{\circ}C\;to\;29.1^{\circ}C$, and total ammonia arround 10 ppm or slightly up. Maintaining phytoplankton bloom was not successful probably because of the active consumption by the heavily stocked tilapia. Several attempts were made by changing the culture water with green water from a nearby earthen pond with results of fading-away in a couple of days. Feed conversions were relatively high ranging from 0.9 to 1.2 except for experiment 1 when the fish were not fully recovered from weakened wintering state. The feed used was partly laboratory prepared $25\%$ protein diet and mostly commercially available $39\%$ protein carp feed. Spawning was completely controlled during the experiment, resulting from density effect, which ranged from 10kg to 40.7kg per square meter with water depth of 0.5 to 0.6m. Space utilization efficiency was very high. Daily net production from the experiment division 3, which showed the highest result, was 6.206 kg per tank, which is calculated 3,235 metric tons per hectare per year, This time, water temperature ranged from 27.8 to $29.1^{circ}C$, average being $28.4^{circ}C$, and total ammonia arround 10 ppm. An estimation for the commercial set-up of the production system based on the results of experiment divisions which had initial stocking rate $15\;kg/m^2$ or up, is made. If the total facility, 8 tanks comprising $56\;m^2$ in surface area, is used for the present study, the yield would become 5,639 kg from 200 day rearing, which would be possible under double sheets vinyl house without additional heating, and it is thought feasible in the economic view point, when 10 or more units are operated.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.19 no.6
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• pp.563-574
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• 1986

The structure of gonads, gametogenesis and reproductive cycle of the jackknife clams, Solen strictus and Solen gordonis were investigated mainly by histological observation. The first species used were monthly sampled at the coastal area of Dadaepo, Pusan, Korea and Naechodo, Kunsan, Korea for one year from February 1982 to January 1983. The second species were monthly sampled at the sand beach of Dadaepo, Pusan, Korea, from February 1982 to January 1983. Sexualities of Solen strictus and Solen gordonis are dioecious, and these species are oviparous. The gonads are irregularly arranged from the subregion of mid-intestinal gland in visceral cavity to reticular connective tissue of foot. The ovary was composed of a number of small ovarian sacs and the testis was composed of several testicular lobuli which from the tubular structure. Early multiplicating oogonium was about $10{\mu}m$ in diamater. Nucleus and nucleolus, at that time, were distinct in appearance. Each of the early growing oocytes made an egg-stalk, connected to the germinal epithelium of the ovarian sac. A great number of undifferentiated mesenchymal tissue and eosinophilic granular cells are abundantly distributed in the ovarian sacs in the early development stages. With the further development of gonad, these tissue and cells gradually disappeared. Then the undifferentiated mesenchymal tissue and eosinophilic granular cells function as nutritive cells in the formation and development of the early stage germ cells. Mature oocytes were free in the lumen of ovarian sacs and gradually become round or oval. Ripe oocyte was about 80 to $90{\mu}m$ in diameter. With the further development of testis, each of the testicular lobuli formed stratified layers composed of spermatogonia, spermatocytes, spermatids and spermatozoa in groups on the germinal epithelium. After spawning, the gonad gradually degenerated, and disorganized completely. Then new differentiated tissues were rearranged next year. The annual reproductive cycle of those species could be classified into five stages; multiplicative, growing, mature, spent, degenerative and resting stage. It seems that the spawning season is closely related to the water temperature, and the spawning of Solen strictus occurs from June to July at above $20^{\circ}C$ in water temperature. The peak spawning season appeared in June at Dadaepo and in July at Kunsan, The spawning of Solen gordonis occurs from May to June with the peak spawning season in June. Percentages of the first maturity in female of Solen strictus ranging from 5.1-6.0 cm and 7.1-8.0 cm in shell length were $50\%$ and $100\%$, respectively.

• Korean Journal of Health Education and Promotion
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• v.21 no.2
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• pp.17-32
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• 2004

The purpose of this study was to evaluate the patterns of stresses arising from various life styles and their related sociodemographic factors in urban residents. The subjects were recruited from the population of two ‘Dong’s (administrative blocks) representative of Daejeon city through stratified cluster random sampling during the period ranging from June I st to Aug. 31 st, 2003. Self-administered questionnaires, including items asking about subjects' sociodemographic characteristics, daily life styles, measurement of stresses by General Health Questionnaire (Goldberg, 1978), were delivered to 396 residents and their responses were analyzed with the following results. 1. Based on the discriminant scores of HPI, 46.7％ of the subjects were found to have less than 4 points (poor life style), whereas 53.3％ had points higher than 5 (good life style). Higher than 5 points were scored more frequently in females than in the male, in the age group of 40's - 60's than the 30's, in the group with spouse than without, in the group with both spouse and offsprings than without, and in the group owning a house than not. 2. Based on the degree of stress, 17.7％ of the subjects were determined to be healthy, 74.5％ were potentially under stress, and 7.8％ were at higher risk of stress. The proportion of healthy individuals were significantly higher in the male, advanced aged group(40's and 60's), the group with higher education years( over highschool), with higher monthly income over two million Won, and with spouse, than their respective counterparts. On the other hand the proportion of the individuals potentially under stress and at higher risk of stress was significantly higher in the female, in the age group of 30's and over 60's, in the group with academic career lower than middle school, with monthly income lower than two million Won, and without spouse. 3. Based on the relation of HPI with degree of stresses, subjects with HPI scores lower than 4 had increased rate of falling into the groups under potential stress and at higher risk of stress, while on the other hand those with over 5 points were found to be healthy in light of stress. 4. Based on the specific relation of each item of HPI with degree of stress, the proportion of healthy individuals was higher in the groups who take appropriate hours of sleep(7-8hours), who take breakfasts everyday, who take physical exercises everyday, who don't smoke, who don't drink alcohol, who take snacks everyday, who are overweight and obese, whereas the proportion of the group under potential stress and at higher risk of stress was higher in their comparable counterparts. 5. The relation of mean scores of HPI with stress scores in both male and female subjects showed negative correlation that the higher HPI scores, the lower stress scores. 6. Multivariate regression analysis to reveal the factors influencing the stress of the subjects showed that for men the significant factors were age, education, presence of job or not, exercise, subjective health status, with the explanatory power of 26.3％. For women, they included educational years, presence of spouse or not, job, owning a house or not, sleeping hours, drinking habit, taking snacks, subjective health status, with the explanatory power of 31.8％. The above study results suggests that stresses of urban residents have significant correlation with daily life styles and this correlation is also remarkably distinguished by different age and sex.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.7
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• pp.902-907
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• 2007

This study was carried out to find the effect of ${\gamma}$ -irradiation on the physical properties of alginic acid and ${\lambda}$-carrageenan solution. Alginic acid and ${\lambda}$-carrageenan aqueous solution were irradiated with $Co^{60}$ ${\gamma}$ -rays in dosages ranging from 3 to 100 kGy, and then the molecular weight, viscosity and color were measured. The molecular weight of alginic acid and ${\lambda}$-carrageenan solution were decreased as the ${\gamma}$ -irradiation dosage increased. In addition, the viscosity of irradiated alginic acid and ${\lambda}$-carrageenan solution were decreased depending upon the ${\gamma}$ -irradiation dosage, too. The high dosage irradiated alginic acid and ${\lambda}$-carrageenan solution's color were changed to yellow. These results suggest that low dosage of ${\gamma}$ -irradiation from 3 to 20 kGy is a very effective method for producing low-molecular alginic acid and ${\lambda}$-carrageenan.

• Journal of Korean Society of Forest Science
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• v.88 no.3
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• pp.352-363
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• 1999

It has been rare to obtain reliable information related to the size of forest land in North Korea. Several sources of forest statistics, ranging from the first map of forest distribution in Korean Peninsula produced in 1910 to official data reported by the North Korea Government in 1997, were gathered and analyzed to define the characteristics of forest cover changes over years. In addition, Landsat satellite data obtained from 1973 to 1993 were processed for the two study areas of the provinces of Pyungyang and Heasan, where the topography and land use pattern are significantly different each other. Using three sets of multitemporal Landsat imagery, land cover ma-ps were produced by computer classification. Although forest statistics reported before 1990 are somewhat inconsistent, they mere gradually decreasing over years. The estimates of 1991 satellite data and the recent statistics reported in 1998 shows very steep decline in forest lands as compared to the ones before 1990. The abrupt decrease of forest lands after 1990 was also found on the detailed analysis of Landsat data for the two study areas of Pyungyang and Heasan. The rapid decline of forest lands may have something to do with the poor economic situation of the country and the continuing natural disasters of severe flooding and drought. Unstocked forest, which was not classified into forest land, was a very distinct and pervasive land cover type that can be easily observed on satellite imagery. Since unstocked forest land in North Korea may be a critical factor for degrading environmental quality as well as for the continuing natural disasters, further analysis is necessary to define the exact extent and the physical characteristics of the cover type.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.453-457
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• 2016

This study investigates the hatching periods and hatchability of the eggs of Pochazia shantungensis at different collection times from 2011 to 2014, and the effect of temperature on the growth of P. shantungensis nymphs in an area of its outbreak. The hatchability of P. shantungensis eggs varies with their collection time; their hatchability in late November was higher than that in March of the next year, but no difference was observed in their hatching periods. The hatching periods of the eggs were 51.2, 31.3, 24.8, 19.4, 17.1, and 19.4 days at 15, 18, 21, 24, 27, and $30^{\circ}C$, respectively. The hatchability was above 70% at temperatures ranging from 18 to $27^{\circ}C$. The hatching time of the overwintered eggs in the Gurye region in Korea was reduced by 9 days from 2011 to 2014. The hatching rate was relatively higher when the average temperature in the winter season was relatively warmer. The dvelopmental periods of the first to fifth nymphs were 82.8, 58.0, 45.8, and 39.6 days at 18, 21, 24, and $27^{\circ}C$, respectively, at the relative humidity of 40~70%, and a photoperiod off 14 h light:10 h dark. The higher the temperature, the shorter the developmental period. At $30^{\circ}C$, all life stages after the fourth nymph died. Thus, the optimum growth temperature was estimated to be $27^{\circ}C$. For all life stages from the egg to the fourth nymph, the relationship between the temperature and developmental rate was expressed by the linear equation Y = 0.0015 X - 0.014. The lower developmental threshold was $9.3^{\circ}C$ and the effective cumulative temperature was 693.3 degree-days. The lower developmental threshold of approximately $3.8^{\circ}C$ was the lowest at the fourth nymph stage.

• Korean Journal of Ichthyology
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• v.18 no.1
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• pp.1-11
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• 2006

Genomic DNA isolated from two geographical populations of pond-smelt (Hypomesus nipponensis) was amplified for RAPD (randomly amplified polymorphic DNA) analysis. The populations were obtained from Chungju (CJ), in the inland area, and Dangjin (DJ), in the vicinity of the West Sea in Korea. Seven arbitrarily selected primers, OPB-06, OPB-10, OPB-13, OPB-17, OPC-09, OPC-17 and OPC-20, were used to generate the shared loci, polymorphic, and specific loci. Three hundred and eighty-three loci observed per primer were identified in the CJ population, and 287 were identified in the DJ population. Among them, 91 polymorphic loci or 23.8% were polymorphic in the CJ population, and 47 (16.4%) in the DJ population. The number of shared loci observed was 198 in the CJ population and 176 in the DJ population. Forty-four and 75 specific loci were detected in the CJ and DJ populations, respectively. Especially, 99 numbers of shared loci by the two populations, with an average of 14.1 per primer, were observed in the two pond-smelt populations. The average bandsharing value between the two geographical pond-smelt populations was $0.700{\pm}0.008$, ranging from 0.600 to 0.846. Compared separately, the bandsharing value of individuals within the CJ population was higher than that of the DJ population. The dendrogram obtained using the data from the seven primers indicated three genetic clusters: cluster 1, CJ 01, 02, 03, 04, 05, 06, 07, 08, 09, 10, and 11; cluster 2, DJ 01, 02, 03, 04, 05, 06, 07, 08, and 09; and cluster 3, DJ 10 and 11. The genetic distance between the two geographical populations ranged from 0.040 to 0.545. Thus, RAPD-PCR analysis revealed a significant genetic distance between the two pond-smelt populations.