• 제목/요약/키워드: rainbow

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Initial Transcriptome Profile of Rainbow Trout (Oncorhynchus mykiss) Liver

  • Kim Soonhag
    • Fisheries and Aquatic Sciences
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    • 제6권1호
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    • pp.41-44
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    • 2003
  • Ninety nine random complementary DNA clones from rainbow trout (Oncorhynchus mykiss) liver cDNA library were partially sequenced as one approach to analyze the transcribed sequences of its genome. Of the sequence generated, $64.0\%$ of the ESTs were represented by 29 known genes. Thirty six clones of the unknown gene products potentially represent 31 unique genes. Serum albumin $(16.1\%)$ was the most abundant in the liver. The structural genes in the liver $(19\%)$ were the highly expressed functional category. This research is helpful to understand tissue specific gene expression profile and basic relationship between tissue and functional categories of the genes.

무지개송어의 바이러스성 출혈성 패혈증에 관한 기초적 연구 (A Basical Study on Viral Haemorrhagic Septicemia of Rainbow trout, Salmo gairdneri)

  • 이근광
    • 한국어병학회지
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    • 제7권1호
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    • pp.7-11
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    • 1994
  • 무지개송어(Salmo gairdneri)의 바이러스성 출혈성 패혈증에 관하여 연구하였다. 병어의 hematocrit값은 정상어보다 매우 낮게 나타났으며, GOT와 GPT값은 정상어보다 병어에서 약간 높게 나타났다. 병어의 혈청과 장기의 마쇄액을 CHSE-214세포에 감염시켜 세포변성 효과를 조사한 결과 감염 24시간 후에 세포변성이 나타났다.

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Immune gene expression following LPS exposure in the gill of rainbow trout, Oncorhynchus mykiss

  • Hong, Su-Hee
    • 한국어병학회지
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    • 제18권3호
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    • pp.287-292
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    • 2005
  • In the present study, immune gene expression of rainbow trout, Oncorhynchus mykiss, against bacterial endotoxin (LPS) was studied in vivo. The expression of prointflammatory cytokine genes (IL-1$\beta$ and TNF-$\alpha$) and IFN-related genes (IRF-I, Mx-3) at gill was assessed by RT-PCR at different time point of day1 and day 3 post-injection. It was shown that the proinflammatory cytokine gene expression at gill was induced 1 day after LPS injection but the expression was not sustained until day 3. Meanwhile upregulated expression of IFN-related genes was found to be only at day 3 post injection, indicating indirect effect of LPS on these genes.

Stimulatory Effects of cyclic AMP on Vitellogenin Induction by Estradiol-17$\beta$ in the Primary Culture of Hepatocytes in the Rainbow Trout Oncorhynchus mykiss

  • Yeo In-Kyu
    • Fisheries and Aquatic Sciences
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    • 제1권2호
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    • pp.153-158
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    • 1998
  • Effects of cyclic (c) AMP and G-protein related reagents (3-isobutyl-l-methyxanthine (IBMX), Forskolin (FSK), cholera toxin (CTX), and pertussis toxin (PTX≫ on estradiol-17$\beta$ induced vitellogenin (VTG) induction were examined in primary hepatocyte cultures in rainbow trout Oncorhynchus mykiss. The addition of IBMX, FSK, or CTX to the incubation medium markedly increased VTG production, while PTX was not effective in stimulating the production. It is well known that cAMP regulates phosphorylation and dephosphorylation through mediation of protein kinase A. These results suggest that VTG production is highly dependent on cAMP state in hepatocytes because of its highly phosphorylated nature.

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Identification of intestinal microflora in rainbow trout

  • Lee, Soon-Deuk;Lee, Yeon-Hee
    • Journal of Microbiology
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    • 제33권4호
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    • pp.273-277
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    • 1995
  • Although trout farming is well established in Korea, very little information is available on the composition of intestinal microflora in rainbow trout (Salmo gairdnerii). In 1994, from October through November, we investigated the composition and succession of intestinal bacteria. As fish grew, total viable counts increased dramatically until 45 days after fertilization when anaerobes started to appear on the media. After this time, they increased steadily. Ten aerobic generic were identified and Gram negative bacteria constituted 85% of total isolates. Among these, Pseudomonas, Eikenella, and Alcaligenes were the three major genera. Six anaerobic genera were isolated and identified. The ratio of anaerobes to aerobes was about 1 : 1 in adult trout and the composition of genera was changed under different conditions.

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열적외선 이미지를 이용한 영상 처리 (Image Processing using Thermal Infrared Image)

  • 정병조;장성환
    • 한국산학기술학회논문지
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    • 제10권7호
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    • pp.1503-1508
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    • 2009
  • 본 논문은 열적외선 카메라 이미지를 영상 처리 기법을 활용하여 실시간으로 구현하는데 그 목적이 있다. 열적외선 영상 데이터는 온도의 변화에 따라 Hot Mapping, Cool Mapping, Rainbow Mapping을 하였으며, 열적외선 이미지의 명암대비 기능을 알아보기 위해 히스토그램 영상처리 기법을 사용하였고, 물체의 구분을 위해서 열적외선 이미지의 에지 부분을 추출하였다. 또한 이미지에서 온도를 추출해 내기 위해 이미지 정보 프로그램을 만들어 온도를 측정할 수 있었다.

Regulation of Cyp 1A1 Gene Expression by Retinoic Acid Receptor, Retinoid X Receptor and Constitutive Androstane Receptor in Rainbow Trout Hepatoma Cells(Rth 149)

  • Kim, Ji-Sun;Yang, So-Yeun;Seo, Mi-Jung;Sheen, Yhun-Yhong
    • 한국독성학회:학술대회논문집
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    • 한국독성학회 2003년도 추계학술대회
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    • pp.136-136
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    • 2003
  • Exposure of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) causes a variety of biological and toxicology effects, most of which are mediated by aryl hydrocarbon receptor (AhR). The ligand-bound AhR as a heterodimer with AhR nuclear translocator (ARNT) binds to its specific DNA recognition site, the dioxin-responsive element (DRE), and it results in increased transcription of CVP1A1 gene.(omitted)

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Genetic Polymorphism among Korean Salmonids Determined by RAPD (Randomly Amplified Polymorphic DNA) Analysis

  • Park, Jung-Youn;Kim, Mi-Jung
    • 한국해양학회지:바다
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    • 제12권2호
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    • pp.102-111
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    • 2007
  • RAPD analyses using 60 OPERON primers and 13 URPs were performed in order to assess the genetic variation and frequency of polymorphisms in Korean salmonids. RAPDS were very reproducible and most useful at the sub-species level. In RAPD analysis, 138 polymorphic bands were detected between Oncorhynchus masou subspecies and 99 bands were generated in two types of rainbow trout. Estimated genetic distances between O. masou subspecies were 0.28794, and between wild rainbow trout and an albino mutant was 0.22786. Each species of salmonid was well characterized using URP 4R, the obtained bands could be useful as a species specific RAPD markers.