[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.5657/fas.2003.6.1.041

Initial Transcriptome Profile of Rainbow Trout (Oncorhynchus mykiss) Liver

Kim Soonhag (National Genome Research Institute, Korea National Institute of Health)

Publication Information

Fisheries and Aquatic Sciences / v.6, no.1, 2003 , pp. 41-44 More about this Journal

Abstract

Ninety nine random complementary DNA clones from rainbow trout (Oncorhynchus mykiss) liver cDNA library were partially sequenced as one approach to analyze the transcribed sequences of its genome. Of the sequence generated, $64.0\%$ of the ESTs were represented by 29 known genes. Thirty six clones of the unknown gene products potentially represent 31 unique genes. Serum albumin $(16.1\%)$ was the most abundant in the liver. The structural genes in the liver $(19\%)$ were the highly expressed functional category. This research is helpful to understand tissue specific gene expression profile and basic relationship between tissue and functional categories of the genes.

Keywords

EST; Expression; Gene; Liver; Transcriptome; Trout; Oncorhynchus mykiss;

Citations & Related Records

Reference

1	Sasaki, Y.F., D. Ayusawa and M. Oishi. 1994. Construction of a normalized cDNA library by introduction of a semisolid mRNA-cDNA hybridization system. Nucl. Acids Res., 22, 987- 992 DOI
2	Velculescu, V.E., L. Zhang, B. Vogelstein and K.W. Kinzler. 1995. Serial analysis of gene expression. Science, 270, 484-487 DOI ScienceOn
3	Adams, M.D., J.M. Kelley, J.D. Gocayne, M. Dubnick, M.H. Polymeropoulos, H. Xiao, C.R. Merril, A Wu, B. olde, R.F. Moreno, A.R Kerlavage, W.R. McCombie and J.C. Venter. 1991. Complementary DNA sequencing: Expressed sequence tags and human genome project. Science, 252, 1651- 1656 DOI
4	Byrnes, L. and F. Gannon. 1990. Atlantic salmon (Salmo seler) serum albumin: cDNA sequence, evolution, and tissue expression. DNA Cell BioI., 9, 647- 655 DOI ScienceOn
5	Liang, P. and A.B. Pardee. 1992. Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science, 257, 967-971 DOI
6	Sanger, F., S. Nicklen and A.R Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Nat'I. Acad. Sci. USA, 74, 5463-5467 DOI ScienceOn
7	Paabo, S. 2001. The human genome and our view of ourselves. Science, 291, 1219-1220 DOI ScienceOn
8	Patanjali, S.R, S. Parimoo and S.M. Weissman. 1991. Construction of a uniform abundance (normalized) cDNA library. Proc. Nat'l, Acad. Sci. USA, 88, 1943-1947 DOI ScienceOn