한국어병학회지 (Journal of fish pathology)

  • 제18권3호
  • /
  • Pages.287-292
  • /
  • 2005
  • /
  • 1226-0819(pISSN)
  • /
  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
권호 표지

Immune gene expression following LPS exposure in the gill of rainbow trout, Oncorhynchus mykiss

  • Hong, Su-Hee (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 발행 : 20051200
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

In the present study, immune gene expression of rainbow trout, Oncorhynchus mykiss, against bacterial endotoxin (LPS) was studied in vivo. The expression of prointflammatory cytokine genes (IL-1$\beta$ and TNF-$\alpha$) and IFN-related genes (IRF-I, Mx-3) at gill was assessed by RT-PCR at different time point of day1 and day 3 post-injection. It was shown that the proinflammatory cytokine gene expression at gill was induced 1 day after LPS injection but the expression was not sustained until day 3. Meanwhile upregulated expression of IFN-related genes was found to be only at day 3 post injection, indicating indirect effect of LPS on these genes.

키워드

참고문헌

  1. Adams, J. L., Semrod, S. D. and Czuprynski, C. J.: Administration of bacterial lipopolysaccharide elicits circulating TNF-a in neonatal calves. J. Clin. Microbiol., 28: 998-1001, 1990
  2. Campos, M., Godson, D., Hughes, H. P. A. and Babiuk, L. A.: Cytokine applications in infectious disease, In: Ruminant Immunology (B. M. Goddeeris, ed.), CRC Press, Boca Raton, FL, 229-235, 1993
  3. Davidson, J., Smith, T. and Martin, S. A. M.: Cloning and sequence analysis of rainbow trout LMP2 cDNA and differential expression of the mRNA. Fish & Shellfish Immunol., 9: 621-632. 1999 https://doi.org/10.1006/fsim.1999.0222
  4. Hong, S., Zou, J., Crampe, M., Peddie, S., Scapigliati, G., BoIs, N., Cunningham, C. and Secombes, C. J.: The production and bioactivity of rainbow trout (Oncorhynchus mykiss) recombinant IL-l$beta$. Vet. Immunol. Immunopathol., 81: 1-14, 2001 https://doi.org/10.1016/S0165-2427(01)00328-2
  5. Hughes, H. P. A. and Babiuk, L. A: Potentiation of the immune response by cytokines. In Handbook of Vertebrate Immunology (P.-P. Pasto ret, P. Griebel, H. Bazin & A. Goaverts, eds). London: Academic Press. pp. 183-202, 1998
  6. Myers, M. J. and Murtaugh, M. P.: Biology of Tumour Necrosis Factor. In the Cytokines Handbook, 2nd ed. (ed. A. W Thomson). Academic Press, London, 121-151, 1994
  7. Nygaard, R., Husgard, S., Sommer, A. I., Leong, J. A. C. and Robertsen, B.: Induction of Mx protein by interferon and double-stranded RNA in salmonid cells. Fish & Shellfish Immunol., 10: 435-450, 2000 https://doi.org/10.1006/fsim.1999.0249
  8. Sergey, E. L., Gayle, D., Flynn, M. C. and PlataSalarnan, C. R.: lL-l$beta$ system (ligand, receptor type I, receptor accessory protein and receptor antagonist), TNF$alpha$, TGF-$beta$ and neuropeptide Y mRNAs in specific brain regions during bacterial LPS-induced anorexia. Brain research Bulletin., 45: 507-515,1998 https://doi.org/10.1016/S0361-9230(97)00437-1
  9. Zou, J., Wang, T, Hirano, I., Aoki, T, lnagawa, H., Honda, T, Soma, G. I., Ototake, M., Nakanishi, T, Ellis, A. E. and Secombes, C. J.: Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss. Dev. Comp. Immunol., 26: 161-172, 2002 https://doi.org/10.1016/S0145-305X(01)00058-1