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Immune gene expression following LPS exposure in the gill of rainbow trout, Oncorhynchus mykiss  

Hong, Su-Hee (Faculty of Marine Bioscience and Technology, Kangnung National University)
Publication Information
Journal of fish pathology / v.18, no.3, 2005 , pp. 287-292 More about this Journal
Abstract
In the present study, immune gene expression of rainbow trout, Oncorhynchus mykiss, against bacterial endotoxin (LPS) was studied in vivo. The expression of prointflammatory cytokine genes (IL-1$\beta$ and TNF-$\alpha$) and IFN-related genes (IRF-I, Mx-3) at gill was assessed by RT-PCR at different time point of day1 and day 3 post-injection. It was shown that the proinflammatory cytokine gene expression at gill was induced 1 day after LPS injection but the expression was not sustained until day 3. Meanwhile upregulated expression of IFN-related genes was found to be only at day 3 post injection, indicating indirect effect of LPS on these genes.
Keywords
Immune; Oncorhynchus mykiss; Lipopolysaccharide; Gill; Interleukin-$1\beta$; TNF-a; Mx-3; IRF-I;
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