The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY (한국해양학회지:바다)

The Korean Society of Oceanography (한국해양학회)
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Genetic Polymorphism among Korean Salmonids Determined by RAPD (Randomly Amplified Polymorphic DNA) Analysis

  • Park, Jung-Youn (Biotechnology Research Institute, National Fisheries Research & Development Institute) ;
  • Kim, Mi-Jung (Biotechnology Research Institute, National Fisheries Research & Development Institute)
  • Published : 2007.05.31
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Abstract

RAPD analyses using 60 OPERON primers and 13 URPs were performed in order to assess the genetic variation and frequency of polymorphisms in Korean salmonids. RAPDS were very reproducible and most useful at the sub-species level. In RAPD analysis, 138 polymorphic bands were detected between Oncorhynchus masou subspecies and 99 bands were generated in two types of rainbow trout. Estimated genetic distances between O. masou subspecies were 0.28794, and between wild rainbow trout and an albino mutant was 0.22786. Each species of salmonid was well characterized using URP 4R, the obtained bands could be useful as a species specific RAPD markers.

Keywords

References

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