• 한국식물병리학회지
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• 제10권1호
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• pp.7-12
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• 1994

Incidence of radish disease caused by Rhizoctonia solani ranged from 1 to 30% in fields located at Hwaseong, Naju and Yeoncheon in Korea during the growing seasons in 1989, 1990 ad 1993. A total of 133 isolates of R. solani was obtained from the diseased seedlings, leaves and roots of radish collected. The fungus was most commonly isolated from the roots. Among 133 isolates of R. solani, 56 isolates were classified as anastomosis group AG-1 by anastomosis test, 37 isolates as AG-2-1, and 40 isolates as AG-4. Among the isolates of AG-1, 26 isolates were grouped as cultural type IA, and the others as cultural type IB. Cultural types IA and IB of AG-1, were isolated from the leaves, AG-2-1 from the roots, and AG-4 from the seedlings, leaves and roots. Pathogenicity tests revealed that the AG-1(IA) isolates were highly virulent on leaves of radish, but avirulent on the seedlings, petioles and roots. The AG-1(IB) isolates were highly virulent on the leaves, but mildly virulent on the seedlings and avirulent or mildly virulent on the petioles and roots. The AG-2-1, isolates were mildly virulent on the leaves and seedlings and mildly or highly virulent on the petioles and roots. The AG-4 isolates were highly virulent on the seedlings and mildly or highly virulent on the leaves, petioles and roots.

• The Korean Journal of Ecology
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• 제19권3호
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• pp.271-282
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• 1996

This study was aimed to observe the effects of municipal sewage sludge as a organic fertilizer on the growth and KDICical omposition of Altari radish (Raphanus sativus). The accumulation rate of heavy metals of the sludge in the radish was also observed. Municipal sewage sludge of Taejon City was applied to the soil of Pai-Chai University Farm to make 6 gradients of sludge contents in the soil. Root weight of Altari radish was affected significantly by N and P contents in the soil. The longest leaves and roots were produced in the control group, in which urea was applied as nitrogen source to the soil. Even though any other sludge-applied groups produced shorter leaves and roots than the control group, the length of the leaves and roots and the weight of the whole plant of the radish tended to increase with the increase of the sludge application. Contents of N, K, Ca and Mg in the radish were not significantly different among those in 6 experimental groups, but P contents in leaves varied among the 6 groups. Zn content of spring Altari radish treated with 200% sludge was 57.6 mg/kg. It is the highest contents among 6 experimental groups. Contents of Zn, Mn and Cd were higher in leaves than in roots, but less than those in market vegetables. From the above results, it is concluded that municipal sewage sludge can be applied as organic fertilizer to the growth of Altari radish. But this sludge could not be applied under our current regulations, which must be reviewed at intra-governmental leavels.

• 한국자원식물학회지
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• 제30권6호
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• pp.579-589
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• 2017

Glucosinolates (GSLs), beneficial secondary metabolites for human health are abundantly present in radish vegetable. Radish is a member of Brassicaceae family and its seed, leaf and root contain very important GSLs. The objective of this study was to determine the variation of individual and total GSL contents in leaves and roots of 44 radish (Raphanus spp.) germplasm (26 R. sativus L., 3 R. raphanistrum, and 15 R. sativus L. var. raphanistroides Makino), and compare the GSL contents between leaves and roots among three Raphanus species. Thirteen GSLs were identified, being the glucoraphasatin (GRS) and glucobrassicin (GBS) the most abundant aliphatic and indolyl GSLs in both the leaves and roots. Variation in individual and total GSL contents was found among the germplasm of three Raphanus species. The GRS content was higher in roots than that of leaves in all three Raphanus species but the GBS content was higher in leaves than roots. GRS was represented 87.0%, 92.7% and 94.7% of the total GSL in roots of R. sativus L., R. raphanistrum and R. sativus L. var. raphanistroides (Makino) germplasm, respectively. Germplasm of R. raphanistrum exhibited the highest (average, $79.5{\mu}mol/g\;dw$) total GSL with a ranged from 62.7 to $92.9{\mu}mol/g\;dw$. The germplasm IT119288, Joseonmu and IT119262 from R. sativus L., RA 504 and K046542 from R. raphanistrum, and Gyeongju-2003-32 (G2003-32) and IT302373 from R. sativus L. var. raphanistroides (Makino) had high total GSL contents and these could be good candidates for developing the functional compounds-rich varieties in radish breeding program.

• 식물병과 농업
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• 제5권1호
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• pp.61-63
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• 1999

Bacterial soft rot was observed on radish grown in Hongcheon, Kanwon-Do, Korea. The soft rot symptoms began as small water-soaked lesions. The water-soaked lesions enlarged rapidly in roots and produced a foul odor. When roots were affected in the field, the shoots also became infected and watery, causing infected plants to wilt, disorganize, and die. The causal organism was isolated from the lesions, and the identified as Erwinia chrysanthemi based on the morphological, physiological and biochemical characteristics. E. chrysanthemi is first described bacterium which causes bacterial soft rot on radish in Korea.

• 한국환경농학회지
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• 제24권3호
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• pp.245-252
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• 2005

The objective of the present work was to determine the corresponding uptake and assimilation of ${NO_3}^-$ in roots and shoots of leafy radish by applying of mixed amino acids (MAA). The amino acids used in this experiment were alanine (Ala), ${\beta}-alanine\;({\beta}-Ala)$, aspartic acid (Asp), asparagines (Asn), glutamic acid (Glu), glutamine (Gln), and glycine (Gly). Leafy radish was grown by conventional fertilization with macro- and micronutrients under controlled conditions. The 15-day-old seedlings were treated 0, 0.3 and 3.0 mM of MAA containing 5 mM ${NO_3}^-$ in growth medium. Nitrate uptake was determined by following ${NO_3}^-$ depletion from the uptake solution. The activity of the enzymes related to the process of ${NO_3}^-$ reduction (NR: nitrate reductase; NiR: nitrite reductase; GS: glutamine synthetase) and the content of ${NO_2}^-\;and\;{ND_3}^-$ were analyzed in shoots and roots. The results of this study showed that ${NO_3}^-$ uptake was inhibited 38% with treatment of 0.3 mM of MAA. However, there was more than three times increase of N03- uptake in 3.0 mM MAA. In addition, the enzymatic activities were positively affected by the high MAA rate. Finally, the ${NO_3}^-$ content was increased slightly both in shoots and roots of leafy radish by MAA treatments.

• Asian-Australasian Journal of Animal Sciences
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• 제32권3호
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• pp.413-420
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• 2019

Objective: This study investigated the effects of dry radish leaf and root on the quality of pork patties during refrigeration storage. Methods: The patties were divided into the following three groups: the control containing 0% dry radish leaf root powder, RL1 containing 0.5% dry radish leaf root powder, and RL2 and RL3 containing 1% and 2% dry radish leaf root powder, respectively. Proximate composition, pH, cooking loss, microbial analysis, lipid oxidation analysis, color, texture profile analysis and sensory test were performed. Results: Moisture, crude protein, and crude ash contents in RL2 and RL3 were significantly higher than those in other groups (p<0.05), whereas crude fat contents in RL2 and RL3 were significantly lower than other groups (p<0.05). Lightness was significantly lower in RL2 and RL3 than in CON (p<0.05). Cooking loss for RL2 and RL3 were significantly lower than those for the other groups (p<0.05). The pH, thiobarbituric acid levels, and total plate counts of RL2 and RL3 were significantly lower than those of CON at days 7 and 14 (p<0.05). Hardness values of RL2 and RL3 were significantly lower than those of CON, whereas chewiness values were higher than those of CON (p<0.05). In addition, the juiciness of RL2 were significantly greater (p<0.05) than those of the other groups. Conclusion: Dried radish leaves and roots improved the proximate composition and quality characteristics of pork patties, providing a basis to produce high-quality patties with extended expiration dates. Thus, dried radish leaves and roots are effective ingredients for health or functional foods.

• Preventive Nutrition and Food Science
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• 제20권2호
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• pp.119-125
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• 2015

The biologically active compounds raphasatin and sulforaphene are formed during the hydrolysis of radishes by an endogenous myrosinase. Raphasatin is very unstable, and it is generated and simultaneously degraded to less active compounds during hydrolysis in aqueous media. This study determined the hydrolysis conditions to maximize the formation of raphasatin and sulforaphene by an endogenous myrosinase and minimize their degradation during the hydrolysis of radish roots. The reaction parameters, such as the reaction medium, reaction time, type of mixing, and reaction temperature were optimized. A stability test for raphasatin and sulforaphene was also performed during storage of the hydrolyzed products at $25^{\circ}C$ for 10 days. The formation and breakdown of raphasatin and sulforaphene in radish roots by endogenous enzymolysis was strongly influenced by the reaction medium, reaction time, and type of mixing. The production and stabilization of raphasatin in radishes was efficient in water and dichloromethane with shaking for 15 min at $25^{\circ}C$. For sulforaphene, the favorable condition was water as the reaction medium without shaking for 10 min at $25^{\circ}C$. The maximum yields of raphasatin and sulforaphene were achieved in a concurrent hydrolysis reaction without shaking in water for 10 min and then with shaking in dichloromethane for 15 min at $25^{\circ}C$. Under these conditions, the yields of raphasatin and sulforaphene were maximized at 12.89 and $1.93{\mu}mol/g$ of dry radish, respectively. The stabilities of raphasatin and sulforaphene in the hydrolyzed products were 56.4% and 86.5% after 10 days of storage in water and dichloromethane at $25^{\circ}C$.

• 식물병연구
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• 제16권2호
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• pp.148-152
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• 2010

무 시들음병에 대한 저항성 검정법을 확립하기 위하여 접종 방법, 침지 시간, 접종원 농도 등의 발병 조건에 따른 '송백무'(감수성)와 '토광무'(중도저항성)의 무 시들음병 발생을 조사하였다. 이병토 접종법, 관주 접종법과 뿌리 침지법 등의 접종 방법에 따른 무 시들음병 발생을 실험한 결과, 이병토 접종법과 관주 접종법에 의해서는 무시들음병이 발생하지 않았다. 뿌리 침지법의 경우, 뿌리 상처 처리구는 무상처 처리구보다 무 시들음병이 더 많이 발생하였으며, 무상처 처리구에서 두 품종의 무 시들음병 발생은 차이를 나타냈다. 뿌리를 포자현탁액에 침지하는 시간(1시간~24시간)에 따른 무 시들음병 발생은 모든 처리구에서 높은 병 발생을 보였으며, 접종 시간이 증가하여도 무 시들음병 발생은 증가하지 않았다. 그러나 포자현탁액의 포자 농도가 증가함에 따라 무 시들음병 발생은 증가하였다. 위의 결과를 이용하여 시판 무 41종 품종의 시들음병 저항성 정도를 검정하였다. 온실에서 14일 재배한 무의 뿌리를 $1.0{\times}10^7$ conidia/ml의 포자현탁액에 2시간 동안 침지하여 실험한 결과, 6종 품종(15%)은 저항성, 22종 품종(53%)은 중도저항성 그리고 13종 품종(32%)은 감수성을 보였다.

• 식물병연구
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• 제29권1호
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• pp.64-71
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• 2023

In October 2021, soft rot disease seriously affected radish crop in Dangjin, Chungcheongnam-do, Korea. The infected radishes were stunted and turned dark green, with yellowish leaf foliage. A slimy, wet, and decayed pith region was observed in the infected roots. The bacterial strain KNUB-03-21 was isolated from infected roots. The biochemical and morphological characteristics of the isolate were similar to those of Pectobacterium brasiliense. Phylogenetic analysis based on the sequences of the 16S rRNA region and the concatenated DNA polymerase III subunit tau (dnaX), leucine-tRNA ligase (leuS), and recombinase subunit A (recA) genes confirmed that the isolate is a novel strain of P. brasiliense. Artificial inoculation of radish with P. brasiliense KNUB-03-21 resulted in soft rot symptoms similar to those observed in infected radish in the field; subsequently, P. brasiliense KNUB-03-21 was reisolated and reidentified. To our knowledge, this is the first report of P. brasiliense as a causal pathogen of radish soft rot in Korea.

• 한국환경보건학회지
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• 제2권1호
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• pp.1-4
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• 1975

The law prohibits use of human excreta without proper treatment since 1968 in Korea, but the rate of infection of parasitic diseases is high among all ages of people. An examination of parasitic eggs in soil and vegetables was performed from April to September, 1974, soil samples were collected near toilet, at gardens, at vegetable farms with excreta used and not used, and strawberry farms in Shindong myon, Chunseong-gun, Kangwon province, and vegetables collected were cabbage, young radish, lettuce, Welshonion, spinach and strawberry. Main findings are as follows 1. Positive samples of Ascaris lumbricoides are 18 out of 25(64%) in the soils near toilet, 9 out of 28(3.2%) in the garden soils and 8 out of 18(44%) in the soils of vegetable farms. 2. Trichocephalus trichiurus and Enterobius vermicularis were detected in 8 soil samples respectively Trichostrongylus orientalis 7(25%) and Ancylostoma duodenale 3(11%) out of in the soils near toilets. 3. Parasitic egg negatives are 62(55%) out of 112 soil samples. 4. Ascaris lumbricoides is found in 11 roots(48%) of cabbage and young radish out of 23 respectively, 14 lettuce(34%) out of 41,4 Welsh onion(40%) out of 10,3 spinach(33%) out of 9, and 2 strawberry(11%) out of 18. 5. Ancylostoma duodenale positives are 2 roots of cabbage(9%) out of 23, 4 young radish (17%) out of 23, and 7 lettuce(17%) out of 41, and negatives of parasitic eggs on vegetables are 62 roots(51%) out of 124 samples.