• Molecules and Cells
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• v.45 no.8
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• pp.588-602
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• 2022

Various RNA-binding proteins (RBPs) are key components in RNA metabolism and contribute to several neurodevelopmental disorders. To date, only a few of such RBPs have been characterized for their roles in neocortex development. Here, we show that the RBP, Rbms1, is required for radial migration, polarization and differentiation of neuronal progenitors to neurons in the neocortex development. Rbms1 expression is highest in the early development in the developing cortex, with its expression gradually diminishing from embryonic day 13.5 (E13.5) to postnatal day 0 (P0). From in utero electroporation (IUE) experiments when Rbms1 levels are knocked down in neuronal progenitors, their transition from multipolar to bipolar state is delayed and this is accompanied by a delay in radial migration of these cells. Reduced Rbms1 levels in vivo also reduces differentiation as evidenced by a decrease in levels of several differentiation markers, meanwhile having no significant effects on proliferation and cell cycle rates of these cells. As an RNA binding protein, we profiled the RNA binders of Rbms1 by a cross-linked-RIP sequencing assay, followed by quantitative real-time polymerase chain reaction verification and showed that Rbms1 binds and stabilizes the mRNA for Efr3a, a signaling adapter protein. We also demonstrate that ectopic Efr3a can recover the cells from the migration defects due to loss of Rbms1, both in vivo and in vitro migration assays with cultured cells. These imply that one of the functions of Rbms1 involves the stabilization of Efr3a RNA message, required for migration and maturation of neuronal progenitors in radial migration in the developing neocortex.

• Archives of Plastic Surgery
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• v.36 no.5
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• pp.642-648
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• 2009

Purpose: Hexadactyly without thumb is a rare congenital anomaly of the hand where six triphalangeal digits are symmetrically distributed without thumb. Contrary to mirror hands, triphalangeal six digits are symmetrically distributed on each side at the midline with well - differentiated carpal bones, extensor tendons, one ulnar and one radius. The authors developed a new surgical technique based on a three - dimensional concept to correct the hexadactyly and applied to 2 cases of hexadactyly with good functional and aesthetic results. Here we document the surgical technique and its result. Methods: A 16 month old male patient visited our clinic with chief complaints of bilateral hexadactyly deformity. On physical examination most radial first and second digits showed no opposition and adduction motion on both side hands. Radiography showed 6 triphalangeal digits with normal development of carpal, radial and ulnar bone. Right side abnormality was corrected by removal of most radial side extra - digit, rotation and migration of 2nd ray to thumb position and creation of 1st web by transposing a mid - palm based rectangular palmar flap as in Snow & Littler procedure which has been being applied for correction of 1st web syndactyly in cleft hand deformity. Seven months later, left side abnormality was also corrected with the same procedure. Results: Postoperative appearances of the both hands were satisfactory. Flexion, extension, opposition and grasping were possible with the pollicized 2nd ray. Pinching power was 3.0 kg 15 months after surgery and 2.5 kg 22 months after in right hand respectively. Conclusion: In correction of hexadactyly deformity, satisfactory aesthetic and relevant functional results can be expected with authors' newly developed technique: removal of most radial digit, rotation and migration of 2nd digit to thumb position as well as creation of the 1st web space by transposition of mid - palm based rectangular flap.

• Korea-Australia Rheology Journal
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• v.19 no.3
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• pp.99-107
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• 2007

A nanofluid is a mixture of solid nanoparticles and a common base fluid. Nanofluids have shown great potential in improving the heat transfer properties of liquids. However, previous studies on the characteristics of nanofluids did not adequately explain the enhancement of heat transfer. This study examined the distribution of particles in a fluid and compared the mechanism for the enhancement of heat transfer in a nanofluid with that in a general microparticle suspension. A theoretical model was formulated with shear-induced particle migration, viscosity-induced particle migration, particle migration by Brownian motion, as well as the inertial migration of particles. The results of the simulation showed that there was no significant particle migration, with no change in particle concentration in the radial direction. A uniform particle concentration is very important in the heat transfer of a nanofluid. As the particle concentration and effective thermal conductivity at the wall region is lower than that of the bulk fluid, due to particle migration to the center of a microfluid, the addition of microparticles in a fluid does not affect the heat transfer properties of that fluid. However, in a nanofluid, particle migration to the center occurs quite slowly, and the particle migration flux is very small. Therefore, the effective thermal conductivity at the wall region increases with increasing addition of nanoparticles. This may be one reason why a nanofluid shows a good convective heat transfer performance.

• Journal of the Korean Society of Visualization
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• v.10 no.3
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• pp.11-15
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• 2012

Inertial migration phenomena of phytoplankton in pipe flows were investigated using a digital holography technique. As the Reynolds number increases, the microorganisms suspended in a pipe flow are focused at a certain radial position which is called equilibrium position or pinch point. In this study, the effects of the size of microorganism and Reynolds number in the range of 1 < Re < 78 on the inertial migration were investigated and the results are compared with those for solid particles under similar experimental conditions. As a result, the equilibrium position for the elastic microorganisms is not so distinct, compared to the solid particles. This results from deformation of elastic body shape caused by shear-gradient of surrounding flow.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2005.04a
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• pp.245-248
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• 2005

During manufacturing thick composite cylinders, large thermal residual stresses are developed and induce catastrophic interlaminar failures. Since the residual stresses are dependent on many process parameters, such as temperature distribution during cure, cure shrinkage, winding tension, and migration of fibers, calculation of the residual stresses is very difficult. Therefore a radial-cut method have been used to measure the residual stresses in the composite cylinders. But the conventional radial-cut method needs to know numerous material properties which are not only troublesome to obtain but also vary with change of fiber arrangement during consolidation. In this paper, a new radial-cut method with cut-cylinder-bending test was proposed and the measured residual stresses were compared with calculated thermal residual stresses. It was found that the new radial-cut method which does not need to know any of material properties gave better estimation of residual stresses regardless of radial variation of material properties. Additionally, interlaminar tensile strength could be obtained by the cut-cylinder-bending test.

• Geomechanics and Engineering
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• v.2 no.2
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• pp.141-156
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• 2010

In the framework of meshfree methods, a new methodology is developed based on radial point interpolation method (RPIM). This methodology is applied to a one-dimensional contaminant transport modelling in the saturated porous media. The one-dimensional form of advection-dispersion equation involving reactive contaminant is considered in the analysis. The Galerkin weak form of the governing equation is formulated using 1D meshfree shape functions constructed using thin plate spline radial basis functions. MATLAB code is developed to obtain the numerical solution. Numerical examples representing various phenomena, which occur during migration of contaminants, are presented to illustrate the applicability of the proposed method and the results are compared with those obtained from the analytical and finite element solutions. The proposed RPIM has generated results with no oscillations and they are insensitive to Peclet constraints. In order to test the practical applicability and performance of the RPIM, three case studies of contaminant transport through the landfill liners are presented. A good agreement is obtained between the results of the RPIM and the field investigation data.

• Korea-Australia Rheology Journal
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• v.19 no.2
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• pp.89-95
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• 2007

The flow characteristics of chicken blood in a micro-tube with a $100{\mu}m$ diameter are investigated using a micro-Particle Image Velocimetry (PIV) technique. Chicken blood with 40% hematocrit is supplied into the micro-tube using a syringe pump. For comparison, the same experiments are repeated for human blood with 40% hematocrit. Chicken blood flow has a cell-free layer near the tube wall, and this layer's thickness increases with the increased flow speed due to radial migration. As a hemorheological feature, the aggregation index of chicken blood is about 50% less than that of human blood. Therefore, the non-Newtonian fluid features of chicken blood are not very remarkable compared with those of human blood. As the flow rate increases, the blunt velocity profile in the central region of the micro-tube sharpens, and the parabolicshaped shear stress distribution becomes to have a linear profile. The viscosity of both blood samples in a low shear rate condition is overestimated, while the viscosity in a high shear rate range is underestimated due to radial migration and the presence of a cell-depleted layer.

• Archives of Plastic Surgery
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• v.46 no.5
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• pp.399-404
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• 2019

Background The objectives of this study were to design polycaprolactone nanofibers with a radial pattern using a modified electrospinning method and to evaluate the effect of radial nanofiber deposition on mechanical and biological properties compared to non-patterned samples. Methods Radially patterned polycaprolactone nanofibers were prepared with a modified electrospinning method and compared with randomly deposited nanofibers. The surface morphology of samples was observed under scanning electron microscopy (SEM). The tensile properties of nanofibrous mats were measured using a tabletop uniaxial testing machine. Fluorescence-stained human bone marrow stem cells were placed along the perimeter of the radially patterned and randomly deposited. Their migration toward the center was observed on days 1, 4, and 7, and quantitatively measured using ImageJ software. Results Overall, there were no statistically significant differences in mechanical properties between the two types of polycaprolactone nanofibrous mats. SEM images of the obtained samples suggested that the directionality of the nanofibers was toward the central area, regardless of where the nanofibers were located throughout the entire sample. Florescence images showed stronger fluorescence inside the circle in radially aligned nanofibers, with significant differences on days 4 and 7, indicating that migration was quicker along radially aligned nanofibers than along randomly deposited nanofibers. Conclusions In this study, we successfully used modified electrospinning to fabricate radially aligned nanofibers with similar mechanical properties to those of conventional randomly aligned nanofibers. In addition, we observed faster migration along radially aligned nanofibers than along randomly deposited nanofibers. Collectively, the radially aligned nanofibers may have the potential for tissue regeneration in combination with stem cells.

• 한국가시화정보학회:학술대회논문집
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• 2005.12a
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• pp.85-88
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• 2005

Experimental study has been conducted to evaluate characteristics of particle laden flows at the ratio of channel diameter to particle diameter (B = 14.9, 21.6 and 55). Particle velocities and radial concentrations are obtained using a microscope Nd:YAG laser and cooled CCD camera. Results show that there are relative velocities between the fluid and the particles at B = 14.9. It is also observed that the particles are accumulated at r=$0.5\∼0.82R$, with R being tile tube radius, and particle migration occurs at small Reynolds number, by comparing with the results obtained in macro scale. This gives optimal factors for designing microfluidic channels for cell or Particle separation, particle focusing, and so on.

• Journal of Power Electronics
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• v.17 no.5
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• pp.1288-1297
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• 2017

The estimation of the remaining useful life (RUL) of lithium-ion (Li-ion) batteries is important for intelligent battery management system (BMS). Data mining technology is becoming increasingly mature, and the RUL estimation of Li-ion batteries based on data-driven prognostics is more accurate with the arrival of the era of big data. However, the support vector machine (SVM), which is applied to predict the RUL of Li-ion batteries, uses the traditional single-radial basis kernel function. This type of classifier has weak generalization ability, and it easily shows the problem of data migration, which results in inaccurate prediction of the RUL of Li-ion batteries. In this study, a novel multi-kernel SVM (MSVM) based on polynomial kernel and radial basis kernel function is proposed. Moreover, the particle swarm optimization algorithm is used to search the kernel parameters, penalty factor, and weight coefficient of the MSVM model. Finally, this paper utilizes the NASA battery dataset to form the observed data sequence for regression prediction. Results show that the improved algorithm not only has better prediction accuracy and stronger generalization ability but also decreases training time and computational complexity.