• Journal of Microbiology and Biotechnology
• /
• 제18권10호
• /
• pp.1672-1677
• /
• 2008

IscR (iron-sulfur cluster regulator) has been reported to be a repressor of the iscRSUA operon, and in vitro transcription reactions have revealed that IscR has a repressive effect on the iscR promoter in the case of [$Fe_{2}S_{2}$] cluster loading. In the present study, the iscR gene from A. ferrooxidans ATCC 23270 was cloned and successfully expressed in Escherichia coli, and then purified by one-step affinity chromatography to homogeneity. The molecular mass of the IscR was 18 kDa by SDS-PAGE. The optical and EPR spectra results for the recombinant IscR confirmed that an iron-sulfur cluster was correctly inserted into the active site of the protein. However, no [$Fe_{2}S_{2}$] cluster was assembled in apoIscR with ferrous iron and sulfide in vitro. Therefore, the [$Fe_{2}S_{2}$] cluster assembly in IscR in vivo would appear to require scaffold proteins and follow the Isc "AUS" pathway.

• Journal of Microbiology and Biotechnology
• /
• 제19권12호
• /
• pp.1520-1526
• /
• 2009

We have identified the iscR (PA3815) gene encoding an iron-sulfur cluster assembly regulator homolog as one of the genes required for peroxide resistance in Pseudomonas aeruginosa PA14. Here, we present the phenotypic characterization of an iscR deletion mutant in terms of KatA expression, stress responses, and virulence. The iscR null mutant exhibited reduced KatA activity at the posttranslational level, hypersensitivity to hydrogen peroxide, and virulence-attenuation in Drosophila melanogaster and mouse peritonitis models. These phenotypes were fully restored by multicopy-based expression of katA. These results suggest that the requirement of IscR in P. aeruginosa is related to the proper activity of KatA, which is crucial for peroxide resistance and full virulence of this bacterium.

• Journal of Microbiology and Biotechnology
• /
• 제17권8호
• /
• pp.1344-1352
• /
• 2007

An opportunistic human pathogen, Pseudomonas aeruginosa, contains the major catalase KatA, which is required to cope with oxidative and osmotic stresses. As an attempt to uncover the $H_2O_2$-dependent regulatory mechanism delineating katA gene expression, four prototrophic $H_2O_2$-sensitive mutants were isolated from about 1,500 TnphoA mutant clones of P. aeruginosa strain PA14. Arbitrary PCR and direct cloning of the transposon insertion sites revealed that one insertion is located within the katA coding region and two are within the coding region of oxyR, which is responsible for transcriptional activation of several antioxidant enzyme genes in response to oxidative challenges. The fourth insertion was within PA3815 (IscR), which encodes a homolog of the Escherichia coli iron-sulfur assembly regulator, IscR. The levels of catalase and SOD activities were significantly reduced in the iscR mutant, but not in the oxyR mutant, during the normal planktonic culture conditions. These results suggest that both IscR and OxyR are required for the optimal resistance to $H_2O_2$, which involves the expression of multiple antioxidant enzymes including KatA.

• Tuberculosis and Respiratory Diseases
• /
• 제40권3호
• /
• pp.236-249
• /
• 1993

연구배경 : 정상 폐상피세포에서는 항상 생성되고 있는 활성산소(oxygen radical)에 의한 유해작용에 노출되어 있고, 이들 유해 산소들은 폐기종과 같은 폐질환의 원인 기전으로 생각되고 있다. 본 연구에서는 이 방법을 이용하여 만든 폐상피세포 단일막에서 전기생리학적인 관점에서 물질의 이동지표인 short-circuit current(Isc)와 조직저항(R)에 대한 활성산소의 하나인 $H_2O_2$(hydrogen peroxide)가 어떤 영향을 미치는지를 연구함으로서 세포생리학적 기전을 구명하고자 한다. 방법 : Tissue culture-treated polycarbonant membrane filter 에서 배양시킨 쥐 제 2 형 폐상피세포 배양 단일막에서 $H_2O_2$의 능동적 이온 이동 (Isc) 과 수동적 용질이동에 대한 조직저항(R)에 미치는 효과를 관찰하였다. 배양 제 3 일과 제 4 일째 단일막을 수정된 Ussing chamber에 설치하고 막 양측에 HEPES-buffered Ringer 용액으로 incubation 하였다. 외부에서 0~100 mM 농도의 $H_2O_2$를 apical 또는 basolateral side에 작용시켜 Isc와 R의 변화를 관찰하였다. 폐상피세포 장벽이 외부의 $H_2O_2$에 대하여 방어작용을 가지는 세포내 catalase 활성도를 측정하고, catalase 억제제인 aminotriazol(ATAZ) 20 mM의 효과도 함께 관찰하였다. 결과 : 이 단일막은 형태학적으로 보아서 in vivo 에서의 포유류 제 1 형 폐상피세포 장벽의 특성을 나타내고 세포들 사이는 tight junction을 이루며(조직저항 R: 2,000 ohm-$cm^2$ 이상) sodium ion의 능동적 이동 (Isc: 5 ${\mu}A/cm^2$)을 보였다. $H_2O_2$는 dose-dependent 양식으로 Isc와 R 모두 감소시켰다. Apical side에 작용하는 $H_2O_2$에 있어서는 60분에 50% 억제하는 농도인 $ED_{50}$는 Isc와 R은 약 4mM 이었으나 basolateral side의 경우는 약 0.04mM 로서 그 작용 강도는 apical에 비하여 약 100배 정도 더 컸다. ATAZ 존재시 apical side의 $ED_{50}$는 0.4mM로 감소하였으나 basolateral side의 경우 변화가 없었다. $H_2O_2$의 제거율은 apical 또는 basolateral side 어느 쪽에 존재하든 같았으며, 세포내 catalase 활성도는세포배양기간이 길어짐에 따라 증가함을 보였다. 결론 : 이상의 실험결과는 basolateral side에 작용하는 $H_2O_2$는 세포내 막구성성분 중 basolateral 측에 존재하는 곳에(예, $Na^+,\;K^+$-APTase) 직접 장애를 미칠 것으로 생각된다. 한편 apical side에 작용하는 $H_2O_2$는 막성분에 도달하기 전에 세포내에 존재하는 catalase에 의하여 대부분 그 작용을 잃게 된다. 결론적으로 Isc와 R로 측정된 폐상피세포 장벽의 특성은 $H_2O_2$에 의하여 손상을 받고 apical side 보다는 basolateral side 측정이 더 손상을 잘 받게 된다.

• 한국통신학회논문지
• /
• 제41권7호
• /
• pp.799-808
• /
• 2016

The IEEE 802.15.7r1 Task Group (TG7r1), known as the revision of the IEEE 802.15.7 Visible Light Communication standard targeting the commercial usage of visible light communication systems which mainly use either image sensors or cameras, is of interest in this paper. The vast challenge in Image Sensor Communications (ISC), as it has been addressed in the Technical Consideration Document (TCD) of the TG7r1, is the Image Sensor Compatibility to support the variety of different commercial cameras available on the market. The on-going ISC standard must adhere to compatible image sensors regulations. This paper brings an inside review of the TG7r1 and an inside look of related works on Image Sensor Communications. The paper analyzes the compatibility features by introducing a revised model of receiver to explain how those features are necessary. One of the most challenging but interesting features is the capability in being compatible to camera frame rates. The variation of camera frame rate is modeled from verified experimental results. Noticeably, three singular approaches to support frame rates compatibility, including temporal approach, spatial approach, and frequency-domain approach, are proposed on the paper along with concise definitions. Those schemes have been presented as valuable proposals on the call-for-proposal meeting series of the TG7r1 recently.

• 한국미생물학회:학술대회논문집
• /
• 한국미생물학회 2006년도 International Meeting of the Microbiological Society of Korea
• /
• pp.69-71
• /
• 2006

• Journal of Information Science Theory and Practice
• /
• 제8권3호
• /
• pp.64-76
• /
• 2020

Scientometrics and social network analysis (SNA) measures were used to analyze the international scientific collaboration (ISC) of All India Institute of Medical Sciences (AIIMS) for a period of 10 years (2009-2018). The dataset consists of 19,622 records retrieved from the Scopus database. The mean degree of collaboration 0.95 implied that researchers of AIIMS tend to collaborate domestically (80.29%) and internationally (14.67%). The data exhibits a hyper authorship pattern, and a medium-size research team consists of 4 to 10 authors who contributed a maximum of 62.08% (12,182) publications. 71.97% of research findings are scattered in journal articles. The most preferred journals published 58.55% of medical literature. An undirected collaboration network is constructed in Pajek to study the ISC of AIIMS during the period 2009-2018 which consists of 179 vertices (Vn) and 11,938 edges. The degree centrality (Dc) identified that the United States of America (Dc - 54; CC - 0.99) and United Kingdom (Dc - 41; 0.98) are the most collaborative countries in the whole network as well as the most influential countries. The Louvain community detection method is used to detect influential research groups of AIIMS. The temporal evolution of ISC of AIIMS studied through scientometrics and SNA measures shed light on the structure and properties of ISC networks of AIIMS. It revealed that AIIMS, India has taken keen steps to enrich the quality of research by extending and encouraging the collaboration between institutions and industries at the international level.

• 한국통신학회논문지
• /
• 제41권8호
• /
• pp.843-850
• /
• 2016

This paper introduces a novel modulation scheme for vehicular communication in taking advantage of existing LED lights available on a car. Our proposed 2-Phase Shift Keying (2-PSK) is a spatial modulation approach in which a pair of LED light sources in a car (either rear LEDs or front LEDs) is used as a transmitter. A typical camera (i.e. low frame rate at no greater than 30fps) that either a global shutter camera or a rolling shutter camera can be used as a receiver. The modulation scheme is a part of our Image Sensor Communication proposal submitted to IEEE 802.15.7r1 (TG7r1) recently. Also, a neural network approach is applied to improve the performance of LEDs detection and decoding under the noisy situation. Later, some analysis and experiment results are presented to indicate the performance of our system

• 제어로봇시스템학회논문지
• /
• 제7권11호
• /
• pp.912-917
• /
• 2001

In this study, we define the measuring method of crank angle precisely using an event and perform a study on the hardware structure and software algorithms which is applicable for the commercial engine. Also we developed a Computer-ECU(Personal computer based electronic control unit) using a computer and a microprocessor, for performing the ignition at a desire position(angle) and for controlling a duty ratio a pulse for ISC(Idle speed control). We applied these algorithms to the modeling which is induced a concept of event and got a better result than a conventional ECU in the state of transient as a result of performing air fuel ratio control in a commercial engine. This technique can be used for the back to improve ECU performance. It the present type of Hybrid I. C voltage regulator is altered to the new type of regulator, we will be surely able to reduce the production cost as well as simplify the design of alternator\`s rear bracket and rectifier part because of the removal of trio diode. Experiment is taken by MS-R004.

• 대한화장품학회:학술대회논문집
• /
• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book I
• /
• pp.73-84
• /
• 2003

Skin aging process on pre-menopausal women is a problem that needs to be prevented as early as possible. The decrease of oestrogen level which is one of the intrinsic factors of the skin aging process will affect the skin biological process, due to oestrogen receptors on the skin. A number of researches conducted on pre-menopausal women with the allocation of oestrogen hormone resulted in delaying the skin aging process. The administration of soy isoflavon, a phytoestrogen found in daily food, on pre-menopausal women is hoped to be able to prevent skin aging process, even clinically or molecular biologically. This research aims to explain the benefit of administering of soy isoflavon on skin aging process. The design of the research is randomised controlled trial (RCT). As many as 60 pre-menopausal women were collected with simple random sampling method. Soy isoflavon is an independent variable, while skin aging process is a dependent variable assessed from the hydration, sebum level, average roughness, depth of wrinkles, skin clarity, length of the telomere. Analysis was conducted using t and MANDVA tests and.the result showed a significance (F = 10,439; p = 0,001) over the allocation of soy isoflavon to the whole variable dependent, including the telomere length and the skin hydration, meant that allocation of soy isoflavon could delay skin aging process.