[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.0906.06028

IscR Modulates Catalase A (KatA) Activity, Peroxide Resistance, and Full Virulence of Pseudomonas aeruginosa PA14

Kim, Seol-Hee (Department of Life Science, Sogang University)
Lee, Bo-Young (Department of Life Science, Sogang University)
Lau, Gee W. (Department of Pathobiology, University of Illinois at Urbana-Champaign)
Cho, You-Hee (Department of Life Science, Sogang University)

Publication Information

Journal of Microbiology and Biotechnology / v.19, no.12, 2009 , pp. 1520-1526 More about this Journal

Abstract

We have identified the iscR (PA3815) gene encoding an iron-sulfur cluster assembly regulator homolog as one of the genes required for peroxide resistance in Pseudomonas aeruginosa PA14. Here, we present the phenotypic characterization of an iscR deletion mutant in terms of KatA expression, stress responses, and virulence. The iscR null mutant exhibited reduced KatA activity at the posttranslational level, hypersensitivity to hydrogen peroxide, and virulence-attenuation in Drosophila melanogaster and mouse peritonitis models. These phenotypes were fully restored by multicopy-based expression of katA. These results suggest that the requirement of IscR in P. aeruginosa is related to the proper activity of KatA, which is crucial for peroxide resistance and full virulence of this bacterium.

Keywords

Pseudomonas aeruginosa; IscR; KatA; iron;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 4 (Related Records In Web of Science)

Reference
Cited By KSCI

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