• Title/Summary/Keyword: purifying

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Pharmacological screening of Alnus japonica and islation of active constituent

  • Kang, Min-Hee;Jeong, Choon-Sik;Jung, Yun-Yang;Hyun, Jin-Ee;Lee, Eun-Bang
    • Proceedings of the PSK Conference
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    • 2003.04a
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    • pp.269.2-269.2
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    • 2003
  • An extract of Alnus japonica (Betulaceae) cortex has been traditionally used for purifying blood, and curing feces containing blood, enteritis, diarrhea, alcoholism and cut wounds. In the preliminary test was carried out for determining whether it has the novel pharmacological activity, the butanol fraction showed significant inhibitory effect on carrageenan-induced paw edema as an acute inflammation, HCI.ethanol-induced gastric lesion and aspirin-ligation gastric ulcer. (omitted)

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A practical approach to handling protein samples under degradation

  • Jeong-Yong, Suh;Sung Hyun, Hong
    • Journal of the Korean Magnetic Resonance Society
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    • v.26 no.4
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    • pp.66-70
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    • 2022
  • Protein structure determination using NMR spectroscopy requires a suite of heteronuclear 3-D NMR experiments that can take a couple of weeks for completion. During the experiments, protein samples may suffer from slow degradation due to co-purifying proteases, which complicates and slows down the assignment procedure. Here we describe a practical protocol to avoid unwanted proteolysis during the experiment.

The Infiltration Velocity of a Sewage Disposal System with Water Plant and Gravel Bed (수초·골재 하수처리장의 투수속도)

  • Chung, Dong Yang
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.6 no.3
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    • pp.29-34
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    • 2003
  • This paper describes the effects of sewage amount, temperature, and years in operation on the infiltration rate of a sewage disposal system. The self-purifying sewage disposal system, which is typically used in rural areas, consists of reeds and fine gravel. Water plants are planted on the gravel bed which provides the habitat for microbes. The basic process is that the gravel bed filters incoming sewage. Thus this system requires the smooth flow of sewage through the gravel. However, the efficiency of the disposal system will be lowered if the gravel bed is clogged with sewage sludge. A three year study shows that infiltration rate slows down significantly until the 7th day, depending on the sewage amount and the temperature. After the 7th day, the infiltration rate remains almost constant. In addition, the infiltration rate decreases as the temperature falls. It also decreases as the number of years in operation increase. But there is no significant change in the infiltration rate after the 7th day, independent of the temperature, the sewage amount, and years in operation. In order to take advantage of high infiltration rate, which improves the efficiency of the disposal system in its early stages, having two gravel beds and using them alternatively will be efficient. This operation method is called intermittent load and makes the disposal system last longer. The water plant roots above the gravel bed make the effective filtration possible because they delay accumulation of the sewage sludge and stabilize the filtration ability.

Adsorption Characteristics of Granular Activated Carbon Filter Used for Drinking Water Purifier (정수기용 입상활성탄소 필터의 흡착특성에 관한 고찰)

  • Baek, Young-Man;Park, Je-Chul;Kim, Hyung-Jin
    • Journal of Environmental Science International
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    • v.17 no.8
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    • pp.899-905
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    • 2008
  • Quality test for activated carbon(AC) filter used for drinking water purifier is now an obligatory test and the standard material for valid purifying amount in water purifier performance test has been changed from residual chlorine to chloroform according to the notice of Ministry of Environment in 2006. Therefore, this study aimed to compare the ingredients of AC filters by confirming chloroform removal rate of AC filter and conducting 4 adsorption tests (Iodine, methylene blue decolorization, phenol value, ADS value) for AC filters provided by manufacturers. With water pressure of $1kgf/cm^2$, 1,500 liters of prepared inflow went through to check chloroform removal rate. As a result, product with removal rate of below 60% from all products. On the other hand, 4 adsorption tests were conducted for filters in the market and filters from manufacturers. None of the products satisfied all 4 tests. In particular, they showed great shortage to the standard in phenol value and ADS value test. However, manufacturers' filter showed much better performance than filters in the market. Also, the result of valid purifying amount test for each of five products of appropriate product and inappropriate product based on filter quality test showed average 4,440 liters for appropriate product and average 2,620 liters for inappropriate product. According to the result, it is shown that the filter with good adsorption also had good chloroform removal efficiency and adsorption efficiency. Therefore, it is expected that customers can screen good quality product through obligatory conduct of filter quality test. However, it is considered that complementation in system is required for future inspection.

Analysis of Growth Indicators of Applied Plants by AHU(Air Handling Unit)-linking with Artificial Soil-based Vegetation Bio-filters (인공토양기반 식생바이오필터의 AHU(Air Handling Unit) 연계를 통한 적용식물의 생육지표 분석)

  • Kim, Tae-Han;Lee, So-Dam;An, Byung-Ryul
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.21 no.3
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    • pp.99-110
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    • 2018
  • Compared to yellow dust coming from China or particulate matter created naturally in spring due to Total Suspended Particulate(TSP), particulate matter in winter season have much more serious effect on human body as they penetrate cell membranes. Although such particulate matter are becoming a social issue, there are no concrete plans on how to reduce them. Air-purifying plants are limited in maintaining the indoor air quality of large area because it is usually difficult to quantify their performance. In order to improve this, a bio-filter that can be connected to air conditioner is suggested as an option. This study seeks to improve air conditioning model-based monitoring method for bio-filters from prior studies and objectify correlations between applied vegetation and growing environment into quantitative indicators. By doing so, this study seeks to provide criteria on plants applied to artificial soil-based vegetation bio-filters and basic information to set air-conditioning features. The study results confirmed significant tendency on the growing stability of each purifying plant in mechanical air-conditioning environment. Among three models selected for bio-filter vegetation models, epipremnum aureum showed high performance in quantitative indicators, including soil moisture, EC, and leaf temperature, etc., indicating that it would assure the highest growing stability in this test air-conditioning environment.

Functional Characterization of the C-Terminus of YhaV in the Escherichia coli PrlF-YhaV Toxin-Antitoxin System

  • Choi, Wonho;Yoon, Min-Ho;Park, Jung-Ho
    • Journal of Microbiology and Biotechnology
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    • v.28 no.6
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    • pp.987-996
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    • 2018
  • Bacterial programmed cell death is regulated by the toxin-antitoxin (TA) system. YhaV (toxin) and Pr1F (antitoxin) have been recently identified as a type II TA system in Escherichia coli. YhaV homologs have conserved active residues within the C-terminus, and to characterize the function of this region, we purified native YhaV protein (without denaturing) and constructed YhaV proteins of varying lengths. Here, we report a new low-temperature method of purifying native YhaV, which is notable given the existing challenges of purifying this highly toxic protein. The secondary structures and thermostability of the purified native protein were characterized and no significant structural destruction was observed, suggesting that the observed inhibition of cell growth in vivo was not the result of structural protein damage. However, it has been reported that excessive levels of protein expression may result in protein misfolding and changes in cell growth and mRNA stability. To exclude this possibility, we used an [$^{35}S$]-methionine prokaryotic cell-free protein synthesis system in vitro in the presence of purified YhaV, and two C-terminal truncated forms of this protein (YhaV-L and YhaV-S). Our results suggest that the YhaV C-terminal region is essential for mRNA interferase activity, and the W143 or H154 residues may play an analogous role to Y87 of RelE.

Optimization of coagulant dosing process in water purification system using neural network (신경회로망을 이용한 상수처리시스템의 응집제 주입공정 최적화)

  • Nam, Ui-Seok;Park, Jong-Jin;Jang, Seok-Ho;Cha, Sang-Yeop;U, Gwang-Bang;Lee, Bong-Guk;Han, Tae-Hwan;Go, Taek-Beom
    • Journal of Institute of Control, Robotics and Systems
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    • v.3 no.6
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    • pp.644-651
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    • 1997
  • In the water purification plant, chemicals are injected for quick purification of raw water. It is clear that the amount of chemicals intrinsically depends on water quality such as turbidity, temperature, pH and alkalinity. However, the process of chemical reaction to improve water quality (e.g., turbidity) by chemicals is not yet fully clarified nor quantified. The feedback signal in the process of coagulant dosage, which should be measured (through the sensor of the plant) to compute the appropriate amount of chemicals, is also not available. Most traditional methods focus on judging the conditions of purifying reaction and determine the amounts of chemicals through manual operation of field experts using Jar-test data. In this paper, a systematic control strategy is proposed to derive the optimum dosage of coagulant, PAC(Polymerized Aluminium Chloride), using Jar-test results. A neural network model is developed for coagulant dosing and purifying process by means of six input variables (turbidity, temperature, pH, alkalinity of raw water, PAC feed rate, turbidity in flocculation) and one output variable, while considering the relationships to the reaction of coagulation and flocculation. The model is utilized to derive the optimum coagulant dosage (in the sense of minimizing turbidity of water in flocculator). The ability of the proposed control scheme validated through the field test has proved to be of considerable practical value.

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Sequence Diversity of a Domesticated Transposase Gene, MUG1, in Oryza Species

  • Kwon, Soon-Jae;Park, Kyong-Cheul;Son, Jae-Han;Bureau, Thomas;Park, Cheul-Ho;Kim, Nam-Soo
    • Molecules and Cells
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    • v.27 no.4
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    • pp.459-465
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    • 2009
  • MUG1 is a MULE transposon-related domesticated gene in plants. We assessed the sequence diversity, neutrality, expression, and phylogenetics of the MUG1 gene among Oryza ssp. We found MUG1 expression in all tissues analyzed, with different levels in O. sativa. There were 408 variation sites in the 3886 bp of MUG1 locus. The nucleotide diversity of the MUG1 was higher than functionally known genes in rice. The nucleotide diversity (${\pi}$) in the domains was lower than the average nucleotide diversity in whole coding region. The ${\pi}$ values in nonsynonymous sites were lower than those of synonymous sites. Tajima D and Fu and Li $D^*$ values were mostly negative values, suggesting purifying selection in MUG1 sequences of Oryza ssp. Genome-specific variation and phylogenetic analyses show a general grouping of MUG1 sequences congruent with Oryza ssp. biogeography; however, our MUG1 phylogenetic results, in combination with separate B and D genome studies, might suggest an early divergence of the Oryza ssp. by continental drift of Gondwanaland. O. long-istaminata MUG1 divergence from other AA diploids suggests that it might not be a direct ancestor of the African rice species.

Study on Manufacture of High Purity TiCl4 and Synthesis of High Purity Ti Powders (고순도 TiCl4 제조 및 이를 활용한 고순도 Ti 분말 제조 공정 연구)

  • Lee, Jieun;Yoon, Jin-Ho;Lee, Chan Gi
    • Journal of Powder Materials
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    • v.26 no.4
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    • pp.282-289
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    • 2019
  • Ti has received considerable attention for aerospace, vehicle, and semiconductor industry applications because of its acid-resistant nature, low density, and high mechanical strength. A common precursor used for preparing Ti materials is $TiCl_4$. To prepare high-purity $TiCl_4$, a process based on the removal of $VOCl_3$ has been widely applied. However, $VOCl_3$ removal by distillation and condensation is difficult because of the similar physical properties of $TiCl_4$ and $VOCl_3$. To circumvent this problem, in this study, we have developed a process for $VOCl_3$ removal using Cu powder and mineral oil as purifying agents. The effects of reaction time and temperature, and ratio of purifying agents on the $VOCl_3$ removal efficiency are investigated by chemical and structural measurements. Clear $TiCl_4$ is obtained after the removal of $VOCl_3$. Notably, complete removal of $VOCl_3$ is achieved with 2.0 wt% of mineral oil. Moreover, the refined $TiCl_4$ is used as a precursor for the synthesis of Ti powder. Ti powder is fabricated by a thermal reduction process at $1,100^{\circ}C$ using an $H_2-Ar$ gas mixture. The average size of the Ti powder particles is in the range of $1-3{\mu}m$.