Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
권호 표지
DOI QR코드

DOI QR Code

A practical approach to handling protein samples under degradation

  • Jeong-Yong, Suh (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Sung Hyun, Hong (Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, Seoul National University)
  • Received : 2022.12.13
  • Accepted : 2022.12.18
  • Published : 2022.12.20
Download PDF
⟨ Previous Next ⟩

Abstract

Protein structure determination using NMR spectroscopy requires a suite of heteronuclear 3-D NMR experiments that can take a couple of weeks for completion. During the experiments, protein samples may suffer from slow degradation due to co-purifying proteases, which complicates and slows down the assignment procedure. Here we describe a practical protocol to avoid unwanted proteolysis during the experiment.

Keywords

Acknowledgement

This work was supported by Cooperative Research Program for Agriculture Science & Technology Development, Rural Development Administration (PJ011112016). The authors thank the high field NMR facility at the Korea Basic Science Institute and the National Center for Inter-university Research Facilities at Seoul National University.

References

  1. S. Y. Ohki and M. Kainosho, Prog. Nucl. Magn. Reson. 53, 208 (2008)
  2. Y. K. Chae, J. Kor. Mag. Reson. Soc. 4, 133 (2000)
  3. P. Guntert, Q. Rev. Biophys. 31, 145 (1998)
  4. T. Kiefhaber, R. Rudolph, H. H. Kohler, and J. Bichner, Nat. Biotech. 9, 825 (1991)
  5. G. L. Rosano, E. S. Morales, and E. A., Ceccarelli, Protein Sci. 28, 1412 (2019)
  6. B. A. Johnson, and R. A. Blevins, J. Biomol. NMR 4, 603 (1994)
  7. H. J. Dyson and P. E. Wright, J. Biomol. NMR 73, 651 (2019)