• Title/Summary/Keyword: pulsed electric field (PEF)

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Introduction of the Magnetic Pulse Compressor (MPC) - Fundamental Review and Practical Application

  • Choi, Jae-Gu
    • Journal of Electrical Engineering and Technology
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    • v.5 no.3
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    • pp.484-492
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    • 2010
  • Magnetic switch is a kind of saturable inductor, which utilizes nonlinearity of the magnetization curve of ferromagnetic materials. The right understanding of the saturation phenomena, magnetic properties, voltage-time product, and switching characteristics of the magnetic switch is essential in designing the magnetic pulse compressor (MPC). In this paper, the historical background of research on the MPC, fundamental physical properties of the magnetic switches, and application fields of the MPC are presented. Further, an in-depth analysis of pulse compression in series and parallel MPCs is incorporated. As practical application examples, a series MPC used for water treatments and a parallel MPC used for pulsed electric field (PEF) inactivation of bacteria are cited.

Pasteurization of Carrot Juice by High Voltage Pulsed Electric Fields with Square Wave Pulse and Quality Change during Storage (고전압 square wave pulse를 이용한 당근 주스의 살균 및 저장 중 품질 변화)

  • Shin, Jung-Kue;Ha, Koo-Yong;Pyun, Yu-Ryang;Choi, Mun-Sil;Chung, Myong-Soo
    • Korean Journal of Food Science and Technology
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    • v.39 no.5
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    • pp.506-514
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    • 2007
  • In this study, carrot juice was treated with high voltage pulsed electric fields (PEF) and the changes in its physical and chemical properties during storage at $4^{\circ}C$ and $25^{\circ}C$ were investigated. The sterility fur bacteria, yeast and mold in carrot juice increased with increasing electric field strength and treatment temperature. While yeast and mold were completely inactivated at 65kV/cm with a treatment time of $200{\mu}s$ in a continuous PEF treatment system, bacteria were reduced by four log cycles. The results also showed that square wave pulse treatment was more effective for inactivating microorganisms than exponential decay pulse, and this effect was more apparent for carrot juice of lower pH. Although we observed significant changes in physical and chemical properties such as soluble solid content, pH, acidity, color, and carotene retention when the PEF treated samples were stored at the ambient temperature $(20^{\circ}C)$, no significant physical and chemical changes were found at the cold storage temperature $(4^{\circ}C)$ during 28 days of storage. The results indicate that the PEF treated carrot juice is appropriate for commercial refrigerated storage.

Effects of High Voltage Pulsed Electric Fields on the Extraction of Carotenoid from Phaffia rhodozyma (Phaffia rhodozyma로부터 Carotenoid 추출에 미치는 고전압 펄스 전기장의 영향)

  • Kim, Nam-Hoon;Shin, Jung-Kue;Cho, Hyung-Yong;Pyun, Yu-Ryang
    • Korean Journal of Food Science and Technology
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    • v.31 no.3
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    • pp.720-726
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    • 1999
  • High voltage pulsed electric fields (PEF) technology is a non-thermal technique which is applicable to extract useful components froms biological materials. This research suggested the possibility for extracting carotenoid pigments from Phaffia rhodozyma by PEF treatments. The yeast cell suspensions were treated with high voltage pulses in a recycled PEF treatment chamber which consists a pair of thin plates of stainless steel adhering to a small chamber with approximately $1{\sim}4\;mm$ gap. A 2.5 log reduction in survivability and more than 98% of electropermeabilization of the yeast cells could be achieved by PEF treatment for $300\;{\mu}s$ with an electric field of 30 kV/cm and pulse duration of $1\;{\mu}s$. When the yeast cell suspended in 0.01% NaCl solution were treated with PEF under various conditions, carotenoid pigments were not extracted. However, the PEF treatment of the yeast cell suspensions in 0.01% $CaCl_2$ solution, have positive effects on the extraction of carotenoid pigments ($27.3\;{\mu}g/g$ of dried yeast).

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Inactivation of Lactobacillus plantarum by High Voltage Pulsed Electric Fields Treatment (고전압 펄스 전기장 처리에 의한 Lactobacillus plantarum의 불활성화)

  • Shin, Hae-Hun;Pyun, Yu-Ryang
    • Korean Journal of Food Science and Technology
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    • v.29 no.6
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    • pp.1175-1183
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    • 1997
  • Lethal effects of high voltage pulsed electric fields (PEF) on suspensions of Lactobacillus plantarum cells in phosphate buffer solution were examined by using continuous recycle treatment system. Critical electric field strength and treatment time needed for inactivation of L. plantarum were 13.6 kV/cm and $16.1\;{\mu}s$ at room temperature, respectively. As decrease in frequency (decreasing pulse number), the degree of inactivation of L. plantarum was increased. A 2.5 log reduction in microbial population could be achieved with an electric field strength of 80 kV/cm, 300 Hz frequency and $2000\;{\mu}s$ treatment time. Survivability was decreased with increase in total treatment time (cycle number) and frequency at the same cycle number. As sterilization model of continuous recycle PEF treatment, $logS=-N_m\;log\;m+B$ and $N_m=k_1\;P_n+k_2$ were established. This model was very well fitted to tile empirical data. The rate of inactivation increased with increase in the processing temperature. The maximum reduction in survivability (5.6 log reduction) was obtained with 80 kV/cm electric field strength at $50^{\circ}C$ for $1000\;{\mu}s$ treatment.

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Production of Ethanol Directly from Potato Starch by Mixed Culture of Saccharomyces cerevisiae and Aspergillus niger Using Electrochemical Bioreactor

  • Jeon, Bo-Young;Kim, Dae-Hee;Na, Byung-Kwan;Ahn, Dae-Hee;Park, Doo-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.545-551
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    • 2008
  • When cultivated aerobically, Aspergillus niger hyphae produced extracellular glucoamylase, which catalyzes the saccharification of unliquified potato starch into glucose, but not when grown under anaerobic conditions. The $K_m\;and\;V_{max}$ of the extracellular glucoamylase were 652.3 mg/l of starch and 253.3 mg/l/min of glucose, respectively. In mixed culture of A. niger and Saccharomyces cerevisiae, oxygen had a negative influence on the alcohol fermentation of yeast, but activated fungal growth. Therefore, oxygen is a critical factor for ethanol production in the mixed culture, and its generation through electrolysis of water in an electrochemical bioreactor needs to be optimized for ethanol production from starch by coculture of fungal hyphae and yeast cells. By applying pulsed electric fields (PEF) into the electrochemical bioreactor, ethanol production from starch improved significantly: Ethanol produced from 50 g/l potato starch by a mixed culture of A. niger and S. cerevisiae was about 5 g/l in a conventional bioreactor, but was 9 g/l in 5 volts of PEF and about 19 g/l in 4 volts of PEF for 5 days.

Non-thermal Pasteurization of Carrot Juice by High Voltage Pulsed Electric Fields with Exponential Decay Pulse (고전압 Exponential Decay Pulse를 이용한 당근주스의 비열(非熱) 살균)

  • Ha, Koo-Yong;Shin, Jung-Kue;Lee, Seok-Hoon;Cho, Hyung-Yong;Pyun, Yu-Ryang
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1577-1582
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    • 1999
  • Carrot juice inoculated with $2\;{\times}\;10^8\;cfu/mL$ of Escherichia coli was treated with pulsed electric fields(PEF) for the purpose of a development of new cold pasteurization processes. Inactivation of E. coli in carrot juice increased with increase in intensity of the electric field strength and treatment time. The cells were suspended at concentration of ca. $2\;{\times}\;10^8$ cells per ml. A reduction of 4D was obtained at 40 kv/cm and 256 exponential decay pulses at room temperature. Critical electric field strength(Ec) and treatment time(tc) needed for inactivation of E. coli were 11.74 kV/cm and $3.6\;{\mu}s$ at room temperature, respectively. The combination of PEF and thermal treatment inactivated E. coli more effectively. The reductions of up to 5.5D were observed when the carrot juice was treated with PEF of 22.5 kV/cm and $205\;{\mu}s$ at $50^{\circ}C$. PFF treatment did not effect in color, pH, $^{\circ}Brix$, titratable acidity and ${\alpha}-,\;{\beta}-carotene$ contents of carrot juice.

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Quality Evaluation of Spent Hen and Broiler with Pulsed Electric Field and Super Heated Steam Treatment (펄스자기장 및 과열증기 처리를 통한 노계육과 육계의 품질 평가)

  • Jeon, Ki-Hong;Hwang, Yoon-Seon;Kim, Young-Boong;Choi, Yun-Sang;Kim, Eun-Mi;Lee, Dong-Un;Choi, Jinyoung
    • The Korean Journal of Food And Nutrition
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    • v.29 no.5
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    • pp.753-759
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    • 2016
  • The processing properties of spent hen and broiler chicken were investigated before and after treatment to improve texture characteristics. Each treatment consisted steaming (S) with $85^{\circ}C$ for 20 min, Pulsed Electric Field (PEF) with 1.5 KV/cm for 4 sec, and Super Heated Steam (SH) with an oven temp. of $300^{\circ}C$, a steam temp. of $350^{\circ}C$ for 8 min. The yield of spent hen and broiler were 66.85% and 63.80% respectively in the control, but decreased in every treatment was lowest at 61.05% in the PEF treatment (p<0.05). In the color test, L value decreased, but the a and b values increased regardless of the species of spent hen or broiler. In the test of heating loss, the S treatment of spent hen had the highest result of 45.25% but lowest of 30.66% in the SH treatment of the broiler. When it was compared with various treatments, SH after PEF treatment showed the better result in terms of heating loss than the PEF or SH treatment respectively. In the test of texture, the broiler showed the lowest hardness of 5.57 kg in the SH (p<0.05). Otherwise, the spent hen resulted in 14.08 kg of hardness in steaming after PEF, but it improved significantly to 10.73 kg in SH after PEF. In the test of 9 scored sensory evaluation of overall palatability, 7.8 point was the best score with SH treatment in the broiler. The best score in spent hen was 6.3 point which was SH after PEF treatment. With this experiment, SH after PEF was the condition in the treatments to have the better texture of spent hen.

Influence of Pulsed Electric Field on Accumulation of Calcium in Lactobacillus rhamnosus B 442

  • Goral, Malgorzata;Pankiewicz, Urszula;Sujka, Monika;Kowalski, Radoslaw;Giral, Dariusz;Kozlowicz, Katarzyna
    • Journal of Microbiology and Biotechnology
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    • v.30 no.1
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    • pp.44-53
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    • 2020
  • Calcium is an element that performs many important functions in the human body. A study was conducted on the use of a pulsed electric field (PEF) to enrich cells of Lactobacillus rhamnosus B 442 in calcium ions. The highest concentration of calcium ions in bacterial cells (7.30 mg/g d.m.) was obtained at ion concentration of 200 ㎍/ml of medium and with the use of the following PEF parameters: field strength 3.0 kV/cm, exposure time 10 min, pulse width 75 ms and 20 h of culturing after which bacteria were treated with the field. Cell biomass varied in the range from 0.09 g/g d.m. to 0.252 g/g d.m., and the total number of bacteria ranged from 1010 CFU/ml to 1012 CFU/ml. Microscope photographs prove that calcium ions were situated within the cells of the bacteria, and electroporation contributed to an increase in the effectiveness of the ion bioaccumulation process. Samples containing calcium and subjected to electroporation displayed intensive fluorescence. The significance of this research was the possibility of using probiotic bacteria enriched with calcium ions for the production of functional food in subsequent studies.

The study of Low Temperature Pasteurization System using High Voltage High Current Pulse Electric Field (고압 대전류 펄스 전계를 이용한 저온 살균장치 시스템 연구)

  • ;;;;Pavlov
    • Proceedings of the KIPE Conference
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    • 1998.07a
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    • pp.162-165
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    • 1998
  • The non-thermal pasteurization of foods using High Voltage High Current Pulsed Electric Fields (HVHC-PEF) is a promising technology and a sound complement or replacement to traditional thermal pasteurization. The conventional thermal method also inactivates bacteria and other microorganisms harmful to humans, but degrades natural color, flavor, texture and nutrients. At this point, a nonthermal pasteurization technique, HVPEF is thought to be a new processing technique which is able to produce a good quality foods nutritional as well as sensuous. In this paper, the system for HVHC-PEF pasteurization is presented. It use square wave pulse instead of exponential ones. So, power rating of system is reduced considerably. Design considerations for 20kV 500A class equipment are analyzed and experimental results are discussed.

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Commercial pasteurization of foods using high voltage pulsed electric fields treatment (고전압 펄스 전기장을 이용한 식품의 상업적 살균)

  • Shin, Jung-Kue
    • Food Science and Industry
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    • v.53 no.3
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    • pp.284-294
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    • 2020
  • High voltage pulsed electric field technology has been attracting attention in the the food industry as an eco-friendly nonthermal process technology using electrical energy. The lack of understanding of the equipment and the burden of equipment cost have not significantly increased the commercial application, but the potential as a technology to replace the heat process has been continuously increased. Sterilization of foods using the PEF process has been applied to liquid foods with low viscosity such as fruit and vegetable juices, but recently, high viscosity smoothies, high concentrate protein drink, mixed juice, and alcoholic beverages. Studies on sterilization of solid foods such as powders, raw meats are also being conducted. Also, the application of extraction and recovery of useful ingredients, activation of active compounds, pretreatment of drying, improvement of meat quality, changes of properties of starch has been studies.