• 대한수의학회지
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• 제38권4호
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• pp.803-810
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• 1998

Pullorum disease caused by Salmonella pullorum, has been considered as one of the most important diseases in both clinically and economically in poultry industry since it had been firstly reported in 1925 in Korea. This disease is still problem in the industry in this country even though several attempts have been made to eradicate the disease. As one of the trials to solve the problem, we investigated the pattern of the outbreak of the disease, isolated the causative agent, S pullorum and tested biochemical properties, antimicrobial susceptibility and plasmid profiles of the isolates. Outbreak of the disease based on the species was the highest in layer followed by in Korean native chick, and broiler. Daily mean mortality in vertical transmission (0.90%) was higher than that in horizontal (0.14%). There was no seasonal difference in the outbreak. Also, biochemical properties and antimicrobial susceptibility pattern of the isolates were same. However four different plasmid profiles of the isolates were observed. These results suggested that S pullorum isolates were different in the genotype while they were same in phenotypes.

• 대한수의학회지
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• 제43권1호
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• pp.77-86
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• 2003

Pullorum disease due to Salmonella enterica subspecies enterica bioserovar Pullorum (S. pullorum) is reported to be an endemic disease in domestic poultry flocks. The pulsed-field gel electrophoresis (PFGE) subtyping method was used to assess the extent of genetic diversity and clonality of most of salmonella serotypes and other diverse bacterial species from animals and environmental samples in worldwide. Nowadays, PFGE has already been evaluated as a gold standards for molecular subtyping of salmonella serotypes compared with other molecular analysis methods. PFGE of XbaI digested chromosomal DNA from 23 strains of S. pullorum gave 5 distinctive pulsotypes (from SXPI to SXPV) with 5% confidence range of Dice coefficients, indicating that PFGE is very discriminative and that multiple clones of S. pullorum have been existed and diffused all of domestic poultry flocks industries since 1995. Two dominant pulsogroups (SXA & SXB) appeared as a major clones in this country, because they had consistently been recovered from diverse sources including both chicken organs and raw feed materials between 1995 and 1998. In addition, the matching percentage of PFGE profiles (PFP) among strains from both chickens and feed ingredients provides indirect evidence of the possible transmission of pullorum disease from contaminated raw feed ingredients for chicken production. In calculating of discrimination index (DI) for PFGE method by Simpson's index, DI was appeared as 0.917. Therefore, this index suggested that the present PFGE would seem to be a desirable and confident molecular typing method for S. pullorum strains. To our knowledge for pullorum disease, this is the first study to compare S. pullorum strains from chicken organs and feed samples using the PFGE.

• 대한수의학회지
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• 제10권2호
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• pp.1-5
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• 1970

In these studies the efficracy of plate, tube agglutination and agar-gel precipitin test were compared for the detection of pullorum infected chickens. From all the chickens showing positive reaction in agar-gel precipitin test, Salmonella pullorum organisms were isolated.

• 한국동물위생학회지
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• 제21권3호
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• pp.313-323
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• 1998

In order to establish a sensitive and specific diagnostic method for detection of antibody to Salmonella pullorum, a enzyme-linked immunosorbent assay(ELISA) was designed and standardized. The diagnostic efficacy of the established ELISA was compared with that of the serum plate agglutination test and immunodiffusion test for pullorum disease. 1. The chicken hyperimmune sera to Salmonella pullorum, S gallinarum, S typhimurium and S typhi were shown the cross reaction to S pullorum antigen by serum plate agglutination test. 2. When compared the cross reaction titer of microplate agglutination test for chickens hyperimmune sera, it was found that the titer were 64 in S pullorum, 32 in S gallinarum, 4 in S typhimurium and 8 in S typhi, respectively. 3. When compared the specificity of various antigen(HA, EA, PA and SA) by the immunodiffusion test, the most suitable antigen was phenol-treated bactrium. 4. The optimal concentration of S pullorum antigen for ELISA was 1 : 160 dilution of bacterium. 5. The efficacy of the ELISA for detection of S pullorum antibody was compared with serum Plate agglutination test and immunodiffusion test in chickens infected with S pullorum. The antibody was first detected at 6 days after infection using three tests examined. The antibody was alldetected at 9 days by ELISA, at 12 days by serumplate agglutination test, at 15 days by immunodiffusion test.

• 대한수의학회지
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• 제41권3호
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• pp.357-365
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• 2001

This study was performed to detect the Salmonella genus-specific DNA marker for comparing of polymophisms between S pullorum and S gallinarum by using PCR amplified techniques. A total of ten primers were used to detect DNA polymorphisms from S pullorum and S gallinarum. The number of DAF bands detected per each primer varied from 26 to 45, with an average of 32.7 using 10 primers. A total of 327 DAF bands were generated and among them 123 bands were polymorphic(37.6%). These DNA amplified fingerprinting(DAF) specific bands for S pullorum and S gallinarum were observed from all primers. For S pullorum, GEN 60-04, GEN 70-04 and GEN 70-03 primers showed a high level of polymorphism with 0.79, 0.70 and 0.57, respectively. But GEN 60-05 primer did not show a level of polymorphism. For S gallinarum, GEN 70-03, 60-04, 60-07, 70-05 and 70-04 primers showed a higher a low level of polymorphism from 0.16 to 0.28. Each five strains of S pullorum and S gallinarum were isolated from chickens showed typical clinical signs related with infection of pullorum disease or fowl typhoid at commercial chicken farms. DNA markers of these strains produced by GEN 70-04, GEN 70-05 and GEN 70-08 showed significant difference of band patterns between S pullorum and S gallinarum. These DNA markers could be used for comparison of DNA marker polymorphism between S pullorum and S gallinarum as well as rapid diagnosis of fowl typhoid and pullorum disease of domestic fowls.

• 한국환경보건학회지
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• 제9권1호
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• pp.95-99
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• 1983

A study on the salmonella pullorum was carried out on 15, 000 hens of breeding chicken, when examined using pullorum antigens, and 165eggs from the isolated breeding to 35among 171carriers used in o serums in Chonnam area during the period from November 22 to December 20, 1982 The results are summarized as follows 1. Distribution on the carriers of pullorum disease was investigated 171 carriers (1.1%) among 15, 000 hens examined. 2. A majority (33.3%) of group D(transovarian transmission) was observed by classification of salmonella groups in the order of group OB (18.8%), group E(18.2%), group $C_1, C_2$ (each 15.2%~), and group OA (12.7%)

• Journal of Microbiology and Biotechnology
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• 제19권9호
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• pp.898-903
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• 2009

Pullorum disease affecting poultry is caused by Salmonella enterica serovar Pullorum and results in severe economic loss every year, especially in countries with a developing poultry industry. The pathogenesis of S. Pullorum is not yet well defined, as the specific virulence factors still need to be identified. Thus, to isolate specific DNA fragments belonging to S. Pullorum, this study used suppression subtractive hybridization. As such, the genome of the S. Pullorum C79-13 strain was subtracted from the genome of Salmonella enterica serovar Gallinarum 9 and Salmonella enterica serovar Enteritidis CMCC(B) 50041, respectively, resulting in the identification of 20 subtracted fragments. A sequence homology analysis then revealed three types of fragment: phage sequences, plasmid sequences, and sequences with an unknown function. As a result, several important virulence-related genes encoding the IpaJ protein, colicin Y, tailspike protein, excisionase, and Rhs protein were identified that may play a role in the pathogenesis of S. Pullorum.

• 대한수의학회지
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• 제10권2호
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• pp.7-12
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• 1970

This study was conducted to abserve the effects of cortisone acetate, estrogenic hormone and tocopherol on the antibody tresponse to both pullorum suspects and negatives of The increase of antibody titer was shown in a few birds after the treatment of either cortisone acetate or estrogenic hormone. There wuas no change in antibody titer of chickens treated with tocopherol.

• 한국동물위생학회지
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• 제20권1호
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• pp.19-26
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• 1997

The present study was conducted to investigate the general epidemiological situations with 18-pullorum infected chickens from Kyongbuk provinces during the period from June 1995 to January 1996. On the Salmonella pullorum isolation tests by rectal swab culture method from infected chickens (386-samples), any Salmonella spp was not isolated from infected live-birds. But 2-S pullorum were isolated of 2-dead chickens(33.3% ) from 6-dead chickens which were positively reacted by serological tests. On the other hand, we could not isolated any Salmonella spp. in any parts of egg-contents ; egg-shell, egg-white and egg-yolks with 25-infected bird eggs. On the tests of antibiogram, 2-S pullorum strains were highly sensitive to GM, AM, SXT, CZ, K, FIM, ENR, C, AN, N, NN, LIN＋SP, CF, TE and PB, respectively and intermediate sensitive to the CB, CFP, CL, S, P and XNL. But 2-strains were resistant to CC, DP, E, L, OX, TLA and TyLO. In the serological tests, pullorum antibody titers of 18-infected birds was from 2.76 to 9.18 with average by the microplate test. During the 6-months, pullorum antibody average titers were not changed generally. To validate the effects of the antimicrobial agent treatments to the serological antibody titers, infected 6-chickens was medicated with 0.5%-futazolidone. The titer of premeditated birds was average 4.26 but after medication with furazolidone, the titers of treated 6-birds was average 4.08.

• 한국가금학회지
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• 제32권1호
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• pp.23-27
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• 2005

가금 추백리와 밀접한 관계가 있는 단백질을 동정하기 위하여 인위적으로 추백리를 유도한 개체와 정상인 개체의 간에서 단백질을 추출하였으며, 2차원 전기영동(2DE)과 mass spectrometry(MS)를 이용하여 차등 발현되는 단백질을 조사하였다. 총 300여개의 단백질 spot들이 관찰되었으며, 이중 발현양에 현저한 차이를 보이는 spot을 MALDI-TOF MS와 protein database search를 통해 분석한 결과, 가금 APOAI (Apolipoprotein A1)으로 확인되었다. 추백리가 감염된 닭의 간에서 APOA1 단백질의 증가는 손상된 혈관의 재생과 밀접한 관계가 있으며 추백리의 감염 여부를 나타내 주는 Bio-marker로서 활용될 수 있을 것으로 사료된다.