• Title/Summary/Keyword: pseudo-science

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In vitro Multiplication through Single-Node Culture of Sea-Milkwort (Glaux maritima L.) (갯봄맞이(Glaux maritima L.) 실생의 단마디배양을 통한 기내증식)

  • Bae, Su-Ji;Kang, Beum-Chang;Jeong, Mihye;Kim, Soochong;Kim, Chang Kil;Han, Jeung-Sul
    • Horticultural Science & Technology
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    • v.34 no.3
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    • pp.461-471
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    • 2016
  • This study was conducted to establish an in vitro propagation system for sea-milkwort (Glaux maritima L.), which is an endangered coastal plant species with high horticultural value. Two phenotypes, 'Red type (RT)' and 'Pistachio type (PT)' based on the colors of stem and flower, were obtained from a personal horticulturist in 2009 and used for this study as plant materials. The stock plants showed typical morphologies in flower, capsule, and seed appearances as previously reported. Low temperature treatment at $4^{\circ}C$ for four or more weeks after in vitro sowing maximized seed germination percentage, indicating that imbibition of seed and subsequent low temperature treatment are crucial for its germination. The in vitro seedlings had phenotypic variation, falling into 'RT' and 'PT' classes like the stock plants. Although slight differences depending on genotype and medium were recognized, the fourth or fifth nodes detached from the in vitro seedlings revealed the best multiplication efficacy when estimated on the basis of total number of nodes of newly developed axillary shoots. In addition, the nodes from 'RT' and 'PT' regenerated the most shoots on medium supplemented with $0.5mg{\cdot}L^{-1}$ BA alone and $0.5mg{\cdot}L^{-1}$ BA plus $0.5mg{\cdot}L^{-1}$ IAA, respectively. The node culture-derived plantlets were well acclimatized in a culture room ex vitro and completed the pseudo-annual life cycle coincident with that in the natural salt march habitat with the current cultivation method of applying fresh water-irrigation under an inland environment. This work represents the first report of in vitro propagation of sea-milkwort. Thus, our study will contribute to exo-habitat conservation and natural habitat restoration of this endangered species in addition to development of a horticultural product.

Rheology Control of Cement Paste for Applying ECC Produced with Slag Particles to Self-Consolidating and Shotcreting Process (고로슬래그 미분말이 혼입된 자기충전 및 숏크리트용 ECC의 개발을 위한 시멘트풀 레올로지 제어)

  • Park, Seung-Bum;Kim, Jeong-Su;Kim, Yun-Yong
    • Journal of the Korea Concrete Institute
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    • v.20 no.1
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    • pp.67-75
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    • 2008
  • An engineered cementitious composite produced with slag particles (Slag-ECC) had been developed based on micromechanical principle. Base grain ingredients were properly selected, and then the mixture proportion was optimized to be capable of achieving robust tensile ductility in the hardened state. The rheological design is performed in the present study by optimizing the amount of admixtures suitable for self-consolidating casting and shotcreting process in the fresh state. A special focus is placed on the rheological control which is directly applicable to the construction in field, using prepackaged product with all pulverized ingredients. To control the rheological properties of the composite, which possesses different fluid properties to facilitate two types of processing (i.e., self-consolidating and shotcreting processing), the viscosity change of the cement paste suspensions over time was initially investigated, and then the proper dosage of the admixtures in the cement paste was selected. The two types of mixture proportion were then optimized by self-consolidating & shotcreting tests. A series of self-consolidating and shotcreting tests demonstrated excellent self-consolidation property and sprayability of the Slag-ECC. The rheological properties altered through this approach were revealed to be effective in obtaining Slag-ECC hardened properties, represented by pseudo strain-hardening behavior in uniaxial tension, allowing the readily achievement of the desired function of the fresh Slag-ECC. These ductile composites with self-consolidating and shotcreting processing can be broadly utilized for a variety of applications, e.g., in strengthening seismic resistant structures with congested reinforcements, or in repairing deteriorated infrastructures by shotcreting process.

Removal Characteristics of Heavy Metals from Aqueous Solution by Recycled Aggregate and Recycled Aggregate/Steel Slag Composites as Industrial Byproducts (산업부산물인 순환골재 및 순환골재/제강슬래그 조합을 이용한 수용액상에서의 중금속 제거 특성)

  • Shin, Woo-Seok;Kim, Young-Kee
    • Applied Chemistry for Engineering
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    • v.26 no.4
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    • pp.477-482
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    • 2015
  • This study examined the adsorption characteristics of heavy metal ions ($Cr^{6+}$, $As^{3+}$) in an aqueous solution using recycled aggregate (RA) and recycled aggregate (RA)/steel slag (SS) composites. The RA and SS are favorable for the absorbent because it contains about 91% and 86.9%, respectively, which are some of the major adsorbent ingredients (CaO, $SiO_2$, $Al_2O_3$ and $Fe_2O_3$) for heavy metal. Kinetic equilibrium of $Cr^{6+}$ and $As^{3+}$ in RA and RA/SS composites reached within 180 min and 360 min, respectively. The kinetic data presented that the slow course of adsorption follows the Pseudo first and second order models. The equilibrium data were well fitted by the Freundlich model and showed the affinity order of $As^{3+}$ > $Cr^{6+}$. The results of $As^{3+}$ also showed that the adsorption capacity slightly increased with increasing pH from 6 to 10. Meanwhile, the adsorption capacity of $Cr^{6+}$ was slightly decreased. From these results, it was concluded that the RA and RA/SS composites can be successfully used for removing the heavy metals ($Cr^{6+}$ and $As^{3+}$) from aqueous solutions.

Adsorption of Pb(II) by Cherry (Prunus x yedoensis) Leaf-Derived Biochar (왕벚나무 잎으로 제조된 바이오차의 Pb(II) 흡착특성)

  • Lee, Myoung-Eun;Hwang, Kyu-Taek;Kim, Sun-Young;Chung, Jae-Woo
    • Journal of the Korea Organic Resources Recycling Association
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    • v.26 no.2
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    • pp.33-41
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    • 2018
  • Large amounts of leaves from street trees fall onto the streets annually and need to be cleaned and treated. Cherry trees are common street trees in Korea. The adsorption characteristics of Pb(II) by cherry leaf (CL) and cherry leaf-derived biochar (CB) were studied through a series of batch experiments. CB was produced through the carbonization of CL at $800^{\circ}C$ for 90 min. Carbonization increased the C content and pH value, while decreased H and O contents. Well developed pore structure was observed at the surface of CB. The pseudo-second order model better described the kinetics of Pb(II) adsorption onto CL and CB, indicating that the rate-limiting step of the heavy metal sorption is chemical sorption. Fast adsorption rates and high adsorption capacities were obtained by the carbonization from CL to CB. Langmuir models better adequately described the Pb(II) adsorption onto CL and CB. Maximum adsorption capacities of Pb(II) expressed by Langmuir constant, $Q^0$ were 37.31 mg/g and 94.34 mg/g, when CL and CB were used as adsorbents, respectively.

A Synecological Study of the Riverside Vegetation of the Upper Stream of Nakdong River, Korea1a - I. Forest and Shrub Vegetation - (낙동강 상류의 하천변 식생의 군락생태학적 연구 - I. 삼림 및 관목성 식생 -)

  • Song, Jong-Suk
    • Korean Journal of Environment and Ecology
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    • v.22 no.4
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    • pp.443-452
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    • 2008
  • This study conducted a survey on forest and shrub vegetation in the riverside of the main stream of the Nakdong River on the southeastern part of Korea based on the method of ZM school of phytosociology. As a result, this study identified the vegetation units as ten associations and communities belonging to three classes, and four communities unclear to the upper units. The identified vegetation units were as follows: A-1 Acero-Fagetea class group, Fraxino-Ulmetaria, undecided alliance: 1) Fraxinus mandshurica community; Zelkovetalia(prov.), Zelkovion(prov.): 2) Zelkova serrata community, A-2 Salicetea sachalinensis, Salicetalia koreensis, Salicion koreensis: 3) Salicetum koreensio-chaenomeloides, 4) Salicetum koreensis, 5) Hemipteletum davidii, 6) Salicetum gracilistylae, 7) Salicetum graciliglandis, 8) Salicetum subfragilis, A-3 Rosetea multiflorae, Dioscoreo-Puerarietalia lobatae, Actinidio-Vition coignetiae: 9) Stephanandra incisa community, 10) Lycium chinense community, A-4 unclear upper units: 11) Clerodendron trichotomum community, 12) Ailanthus altissima community, 13) Robinia pseudo-acacia community. 14) Amorpha fruticosa community. The community units identified by the study corresponded with the result of cluster analysis quite nicely while the result of PCA analysis in multivariate analyses showed less correspondence with cluster analysis result, but it was interpreted as a degree of aggregation according to anthropogenic influence. The vegetation unit of forests and shrubs, unlike the herbal community in highly common with those of Japan, included five vegetation units unique to Korea at a collective level. On the basis of the above synecological study, this research discussed the relation between each community and environment, and natural environment, etc.

Development of Revegetation Methods Using Fresh Woodchip from Construction Works (건설현장 발생재를 활용한 비탈면 녹화에 관한 연구)

  • Nam, Sang-Jun;Kim, Kyung-Hoon;Yeo, Hwan-Joo;Jung, Ji-Jun
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.7 no.3
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    • pp.86-95
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    • 2004
  • This study was conducted to develop recycle revegetation methods for the restoration of the steep slopes by using fresh wood chip from construction sites. In general, the fresh wood chips can be used as soil media for the restoration works, because they can increase infiltration of rainfall and give enough porous to breathe and elongate for the root growth as well as economic value. The experiment was carried out to compare the effect of fresh wood chips from different mixing with soil, organic material and macromolecular compound which used for slope restoration works conducted by Hyunwoo green(Ltd.). The main results by monitoring for two years are summarized as follows; 1. The soil media made with low percentage of fresh wood chip covered quickly by herb plants. Especially, the soil mixture Type C (wood chip 20%) showed 80 percent ground coverage within two months after seeding. 2. The soil mixture type E (wood chip 40%) and type F (wood chip 50%) which contains more fresh wood chips than soil type C was under 30 percent ground coverage because wood plants are germinated well. If the restoration works aims at making forest, then the soil type E and F would be recommended than using soil type C. 3. Among the woody plants, Ailanthus alfissima, Pinus rigida, Pinus densiflora, and Albizzia julibrissin showed high percentage of germination rates and vigorous growth. In case of shrubs, Lespedeza cyrtobotria and Indigofera pseudo-tintoria scored high percentage of germination rates. 4. In native plants, Chrysanthemum indicum, Artemisia princeps, Lutos corniculatus and Imperata cylindrica showed high percentage of appearance. In case of introduced herbs, Coreopsis lanceolata, Coreopsis tinctoria and Oenothera oborata grew so vigorously. 5. The soil types which including fresh wood chips over 30-40 percentage showed the most diverse plant composition and the most effective germination rates and growth pattern with woody plants. 6. This works to develop recycle revegetation methods using fresh wood chips need more efforts for monitoring the exact effect of fresh wood chips as the soil media.

The Effect of UV Intensity and Wavelength on the Photolysis of Triclosan (TCS) (광반응을 이용한 Triclosan 분해에서의 UV 광세기와 파장의 효과)

  • Son, Hyun-Seok;Choi, Seok-Bong;Khan, Eakalak;Zoh, Kyung-Duk
    • Journal of Korean Society of Environmental Engineers
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    • v.27 no.9
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    • pp.1006-1015
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    • 2005
  • We investigated the effect of hydroxyl radicals on the photolysis of triclosan (TCS), which is a potent broad-spectrum antimicrobial agent. TCS degradation during the initial reaction time of 5 min followed a pseudo-first order kinetic model ai all light intensities at a wavelength of 365 nm and at the low light intensities at a wavelength of 254 nm. The photodegradation rate significantly increased with decreasing wavelength and increasing the UV intensities. The activity of hydroxyl radicals was suppressed when methanol was used as the solvent instead of water. An increase in the photon effect was observed when the UV intensity was higher than $5.77{\times}10^{-5}$ einstein $L^{-1}min^{-1}$ at 254 nm, and lower than $1.56{\times}10^{-4}$ einstein $L^{-1}min^{-1}$ at 365 nm. The quantum yield efficiency for the photolysis of TCS was higher at 365 nm than at 254 nm among the above mentioned UV intensities. Dibenzodichloro-p-dioxin (DCDD) and dibenzo-p-dioxin were detected as intermediates at both UV intensities of $1.37{\times}10^{-4}$ and $1.56{\times}10^{-4}$ einstein $L^{-1}min^{-1}$ at 365 nm. Dichlorophenol and phenol were also detected in all cases. Based on our findings, we presented a possible mechanism of TCS photolysis.

Modification of IKONOS RPC Using Additional GCP (지상기준점 추가에 의한 IKONOS RPC 갱신)

  • Bang, Ki-In;Jeong, Soo;Kim, Kyung-Ok;Cho, Woo-Sug
    • Journal of Korean Society for Geospatial Information Science
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    • v.10 no.4 s.22
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    • pp.41-50
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    • 2002
  • RPM is the one of the sensor models which is proposed by Open GIS Consortium (OGC) as image transfer standard. And it is the sensor model for end-users using IKONOS, a commercial pushbroom satellite, imagery which provide about 1m ground resolution. Parameters called RPC which is IKONOS RFM coefficients are serviced to end-users. But if some users try to make additional effort to get rigorous geo-spatial information, it is necessary to apply mathematic or abstract sensor models, because vendors don't offer any ancillary data for physical sensor models such as satellite orbit and navigation. Abstract sensor models such as pushbroom Direct Linear Transform (DLT) require many GCPs well distributed in imagery, and mathematic sensor model such as RFM, polynomials need much more GCPs. Therefore RPC modification using additional a few GCPs is the best solution. In this paper, two methods are proposed to modify RPC. One is method to use pseudo GCPs generated in normalized cubic, and another method uses parameters observations and a few GCPs. Through two methods, we get improvement of accuracy 50% and over.

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Comparison of Species Distribution Models According to Location Data (위치자료의 종류에 따른 생물종 분포모형 비교 연구)

  • Seo, Chang-Wan;Park, Yu-Ri;Choi, Yun-Soo
    • Journal of Korean Society for Geospatial Information Science
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    • v.16 no.4
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    • pp.59-64
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    • 2008
  • We need to use the strength of each Species Distribution Model(SDM) because presence location data were only collected due to time and economic limitations in Korea. This study investigated and compared GAM(Generalized Additive Model) which is one of presence-absence models with Maxent(Maximum Entropy Model) which is one of presence only models according to location data(presence/absence data). The target species was Fisher(Martes pennanti) which is an endangered species in California, USA. We implemented environmental data such as topography, climate and vegetation, and applied models to sub-regions and study area. The results of this study were as follows. Firstly, GAM which used real presence and absence data was better than GAM which used pseudo-absence data and Maxent which used presence-only data. Secondly, Maxent was better than GAM when presence-only data were used. Lastly, each model which applied to different regions didn't predict other area well due to the difference of habitat environment and over-predicted outside of study area. We need to select an optimal model to predict a suitable habitat according to the type and distribution of location data.

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Feasibility of Coculture Method for Production of Chimeric Mice Using J1 Embryonic Stem Cells

  • Shin Hye-Jun;Park Sung-Sik;Kim Sun-Uk;Cho Sang-Mi;Han Ying-Hao;Kim Hyun-Sun;Kim Sang-Geun;Lee Dong-Seok;Yu Dae-Yeul
    • Biomedical Science Letters
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    • v.10 no.4
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    • pp.391-395
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    • 2004
  • The demand for the production of gene-defective mice from embryonic stem (ES) cells is increasing to clarify decisive gene function in vivo. Although blastocyst injection is widely used to generate ES cell-mediated knockout mice, coculture method has been alternatively used because of several advantages, such as low cost and simple procedure. Thus, this experiment was designed to demonstrate the feasibility of the coculture method using J1 ES cells, which are known to be efficient for blastocyst injection. Eight-cell embryos were harvested from 2.5 days post-coitum (dpc), denuded with acid tyrode's solution, and transferred onto trypsinized J1 ES cells. Aggregation was carried out following two typical methods, which are simple coculture method and aggregation in groove prepared by aggregation needle. Successfully aggregated-embryos were developed to blastocysts for 24 h and transferred into uterus of pseudo-pregnant foster mother. Chimeric offspring was judged by coat pigmentation. In this study, we could obtain chimeric mice from all the two aggregation methods, but the chimera production efficiencies in coculture using groove were three times higher at least than those in the other group. In conclusion, these observations suggest that coculture method should be available for production of knockout mice from J1 ES cells. Presently, the germ-line transmission rates of the chimeras produced from the two methods are under investigation.

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