• Animal Bioscience
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• v.34 no.4
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• pp.613-620
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• 2021

Objective: This study aimed at studying the potential use of Flemingia (Flemingia macrophylla) as a protein source fodder to improve nutrients digestibility and ruminal fermentation efficiency in beef cattle. Methods: Four, Thai native beef cattle were randomly assigned in a 4×4 Latin square design. Four levels of Flemingia hay meal (FHM) were used to replace soybean meal (SBM) in the concentrate mixtures in four dietary treatments replacing levels at 0%, 30%, 60%, and 100% of SBM. Results: The experimental findings revealed that replacements did not effect on intake of rice straw, concentrate and total dry matter (DM) intake (p>0.05). However, the apparent digestibilities of DM, organic matter, crude protein, acid detergent fiber, and neutral detergent fiber were linearly increased up to 100% replacement levels. Moreover, the production of total volatile fatty acids, and propionate concentration were enhanced (p<0.05) whereas the concentration of acetate was reduced in all replacement groups. Consequently, the CH4 production was significantly lower when increasing levels of FHM for SBM (p<0.05). Furthermore, rumen bacterial population was additionally increased (p<0.05) while protozoal population was clearly decreased (p<0.05) in all replacement groups up to 100%. In addition, microbial nitrogen supply and efficiency of microbial nitrogen synthesis were enhanced (p<0.05), as affected by FHM replacements. Conclusion: The findings under this experiment suggest that 100% FHM replacement in concentrate mixture enhanced rumen fermentation efficiency, nutrients digestibilities, bacterial population, microbial protein synthesis, and subsequently reduced CH4 production in beef cattle fed on rice straw.

• Journal of Animal Science and Technology
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• v.47 no.6
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• pp.1081-1086
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• 2005

This study was conducted to investigate the effect of mixture types which consist of different species on the dry matter(DM) yield, botanical composition and forage quality. The experimental design includes three mixture types: Conventional mixtures(orchardgrass 50% + tall fescue 20% + perennial ryegrass 10% + Kentucky bluegrass 10% + white clover 10%), complex mixtures(orchardgrass 40% + tall fescue 20% + perennial ryegrass 10% + Kentucky bluegrass 10% + redtop 10% + alfalfa 5% + red clover 5%) and simple mixtures(orchardgrass 80% + red clover 20%). The DM yield was higher in conventional mixtures(13,070kg/ha) than in other mixtures(p<0.05). In the chemical composition and dry matter digestibility, there was significant difference among mixtures. Crude protein content and dry matter digestibility were higher in complex mixtures than in other mixtures(p<0.05). But, the content of fibrous constituents was higher in conventional mixtures than in other mixtures. The yield of crude protein dry matter(CPDM) and digestible dry matter(DDM) were higher in complex mixtures than in other mixtures. In this experiment, DM yields and quality of mixture types were observed significant difference. therefore, the complex mixtures which combined various species were more effective in enhancing the dry matter digestibility(DMD) and digestible dry matter(DDM) yield.

• Journal of Ginseng Research
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• v.32 no.1
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• pp.39-47
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• 2008

UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. These phenomena in complex skin tissue is controlled with receptor of cell surface growth factor and cytokine receptors. The activation of receptors induces multiple downstream signaling pathways including expression of MMPs (matrix metalloproteinases). This study was aimed to elucidate the mechanism for anti-wrinkle activity of Korean red ginseng, Torilis fructus and Corni fructus mixture (KTNG0345). In this animal study, we have investigated decreasing effects of Korean red ginseng mixture on MMP-3 synthesis through diminishing $TNF-{\alpha}$ signaling that express MMP-1, -3, and -9. c-Jun and c-fos as a component of transcription factor AP-1 (activator protein-1) were analyzed the expression level using real time PCR and western blotting. c-Jun was decreased dose dependent manner both gene and protein level where as cfos was not changed. In upstream, JNK and PAK was not changed, but p38 was decreased in down stream. MMP-3, final product in this pathway was significantly decreased in dose dependent manner. These results suggest that Korean red ginseng mixture have a anti-wrinkle activity through $TNF-{\alpha}$ mediated MMPs expression pathway.

• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.4
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• pp.207-214
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• 2006

The object of this experiment was to suggest the optimum utilization upon rye and hairy vetch crops in Daejeon area. The field trials were conducted from 2002 to 2004 at Chungnam National University in order to evaluate the dry matter yield and quality of rye, hairy vetch, and rye+hairy vetch mixtures. The experiment was designed with split plots (main plot : seeding rate as rye mono culture, hairy vetch monoculture, rye 80%+hairy vetch 20%, rye 60%+hairy vetch 40%, and sub plot : 4 cutting stages as boot, heading, anthesis, milk). The average DM yield for 2 years had a difference on seeding rate and harvesting stages. The DM yield of rye monoculture was higher than those of the other seeding rates. There were significant increases in advanced stages in all treatments. In chemical composition, they also had a difference on seeding rates and growth stages. Hairy vetch monoculture was higher in crude protein (CP) and dry matter digestibility (DMD), but lower in fibrous compound than those of the other seeding rates (p<0.01). The crude protein dry matter (CPDM) and digestible dry matter yield was higher in rye 80%+hairy vetch 20% mixture when it was harvested at anthesis to milk stages. The results of this experiment indicated that when it was harvested at anthesis to the milk stages, rye 80%+hairy vetch 20% mixture was more effective in enhancing the DM yield and forage quality in Daejeon area.

• Journal of The Korean Society of Grassland and Forage Science
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• v.18 no.4
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• pp.355-360
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• 1998

This study was conducted to investigate the effects of mixture of different maturity groups on dry matter yield and nutritive value of oat(var. Foothill and Swan) and rape(var. Akela, Ramon and Ember) in the Fall at the Experimental field of Woosuk University, Wanju, in 1998. The results obtained are summarized as follows : 1. Plant height of Foothill was very slightly higher than that of Swan in oat, but there was no significant difference. Also, although there was no significant difference, there was high in plant height of Ember and Akela with Foothill, and Ramon with Swan. 2. Dry matter(DM) yield of Swan was very slightly higher than that of Foothill in oat, but there was no significant difference. Although there was no significant difference, dry matter yield of Akela was the highest in both oats. 3. Acid detergent fiber and neutral detergent fiber content of Swan were very slightly higher than that of Foothill in oat. And it was slightly higher in Akela or Ramon with Foothill, and it was greatly higher in Ember with Swan. 4. In vitro dry matter digestibility(IVDMD) content of Foothill was very slightly higher than that of Swan in oat. And it was slightly higher in Ember with Foothill, and it was slightly higher in Akela with Swan. Also, in vitro digestible dry matter(IVDDM) of Foothill was very slightly higher than that of Swan in oat. And it was slightly higher in Akela with Foothill and Swan. 5. Crude protein(CP) content and crude protein(CP) yield of Foothill were very slightly higher than that of Swan in oat. And it was slightly higher in Akela with Foothill and Swan. The results demonstrated that although there was not significant difference, the mixture of var, Foothill of oat and var, Akela of rape in the Fall was very good for nutritive value and DM yield.

• Journal of Nutrition and Health
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• v.38 no.5
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• pp.352-363
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• 2005

The previous extensive in vitro studies on the antioxidative activities of a number of Korean grains, vegetables, seaweeds and mushrooms, and the various combinations of these food source exhibited a wide range of antioxidative activities, and four food mixtures composed of 5 kinds of foods (5A, 5B, 5C and 5D) were designed from 16 selective foods showing. high antioxidant effect, in vitro, to find the good combinations for the meal planning. Mixture 5B or 5C contained very high levels of total flavonoid and polyphenol, and ethanol extract from 5A, 5B or 5C showed very strong inhibitory effects against in vitro $Fe^{2+}-induced$ lipid peroxidation and ethanol extract from 5B or 5C showed remarkable DPPH radical scavenging effect and lipid peroxide-protein conjugation inhibition effect. And in vivo study was also carried out with two mixtures (5B, 5C). Powders (P5B, P5C) or ethanol extracts (E5B, E5C) of these mixtures were supplemented to Sprague-Dawley rats fed on high fat $(15\%)-high$ cholesterol $(1\%)$ semipurified diet for 5 weeks. The total antioxidant power in serum was significantly higher in P5B, P5C, E5B and E5C groups than in high fat control group, and $ascorbate-Fe^{2+}-induced$ TBARS was significantly lowered by E5B supplementation in rat liver. In liver tissue, Cu, Zn-SOD activity was significantly higher in P5B and E5B groups than in high fat control group, while catalase or GSH-peroxidase (GPx) activity was not changed by any supplementations. In kidney, Cu, Zn­SOD activity was significantly higher in P5B group than in high fat control group, while GPx activity was not changed by any supplementations. Taken together, mixture 5B and 5C showed very strong antioxidative effects both in vitro and in vivo. Therefore, the ingredient Korean foods of 5B and 5C could be recommended to take a lot together for prevention from age-related chronic diseases.

• Biomolecules & Therapeutics
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• v.18 no.1
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• pp.39-47
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• 2010

Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor involved in neuronal differentiation, plasticity, survival and regeneration. BDNF draws massive attention mainly due to the potential as a therapeutic target in neurological diseases such as depression and Alzheimer's disease. In a primary screening for the natural compounds enhancing BDNF release from cultured rat primary cortical neuron, we found that compounds such as baicalein, tanshinone IIa, cinnamic acid, epiberberine, genistein and wogonin among many others increased BDNF release. All the compounds at $0.1{\mu}M$ of concentration barely showed stimulatory effect on BDNF induction, however, their combination (mixture 1; baicalein, tanshinone IIa and cinnamic acid, mixture 2; epiberberine, genistein and wogonin) showed synergistic increase in BDNF release as well as mRNA and protein expression. The level of BDNF expression was comparable to the maximum BDNF stimulation attainable by a positive control oroxylin A ($20{\mu}M$) without cell toxicity as determined by MTT analysis. Both mixtures synergistically increased the phosphorylation of extracellular signal-regulated kinase (ERK) as well as cAMP response element binding protein (CREB), an immediate and essential regulator of BDNF expression. Similar to these results, mixture of these compounds synergistically inhibited the up-regulation of inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide treatments in rat primary astrocytes. These results suggest that the combinatorial treatment of natural compounds in lower concentration might be a useful strategy to obtain sufficient BDNF stimulation in neurological disease condition such as depression, while minimizing potential side effects and toxicity of higher concentration of a single compound.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.10
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• pp.1446-1458
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• 2013

The study assessed the effect of dietary supplementation of leaf meal mixture (LMM) containing condensed tannins (CT) on feed intake, nutrient utilization and performance of sheep infected with Haemonchus contortus. Eighteen adult sheep of similar age and body weight ($25.03{\pm}1.52$) were included in this study and out of these, 12 sheep were infected with single dose of infective third stage larvae of H. contortus at 2,000 larvae per sheep. The experimental sheep were allocated in three different groups' i.e. negative control (NC; no infection), control (C; H. contortus infected) and treatment (T; H. contortus infected+CT at 1.5% of the DM through LMM) and the experiment was conducted for a period of 90 d. The intake of dry matter (DM), organic matter (OM) and digestibility of DM, OM, neutral detergent fibre (NDF) and acid detergent fibre (ADF) were comparable among three animal groups. However, digestibility of crude protein (CP) and ether extract (EE) were significantly (p<0.05) higher in NC group as compared to both C and T groups. Nitrogen (N) retention (g/d or % of N intake) was significantly (p = 0.038) lower in C group as compared to T and NC groups. Daily intake (g/kg $W^{0.75}$) of digestible crude protein (DCP), digestible organic matter (DOM) and total digestible nutrient (TDN) did not differ significantly (p<0.05) in the three groups. Haemoglobin (Hb) and packed cell volume (PCV) were significantly (p<0.001) higher in treatment group as compared to control. The level of Hb and PCV reduced (p<0.001) after 30 days of experimental feeding. CT significantly (p<0.001) reduced serum urea in T group as compared to NC and C groups. Serum proteins differed significantly (p<0.01) among the three groups. The activity of serum enzymes AST, ALT, ALP and LDH were also statistically non significant (p<0.05) among treatments. The weight of abomasal lymph nodes (ALN) in T group was higher (p<0.05) than in C group. Treatment group had lower (p<0.05) total worms and fecal egg count compared to control group. It may be concluded that dietary supplementation of CT through LMM significantly improved the N retention, and inhibited the different developmental stages of Haemonchus contortus in experimental sheep.

• Journal of the Korean Society of Food Science and Nutrition
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• v.17 no.3
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• pp.191-197
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• 1988

Sorption properties of corn starch(CS) and Soy protein isolates(SPI) in plastic films packaging were investigates for binary system. The mixture were sealed in plastic films of low density polyethylene(LDPE), oriented polypropylene(OPP) and LDPE/OPP coated film and packaging effect on the changes of moisture sorption during storage at $40^{\circ}C$ were studied. The following results were obtained. The water vapor permeability of material films was $32.6g/m^2/24hrs(below\;g)$ for 0.02mm LDPE film, 14.01g for 0.04mm LDPE film, 7.30g for 0.02mm OPP film, 3.37g for 0.04mm OPP film and 4.869 for 0.02mm LDPE/0.02 mm OPP confine film at $40^{\cire}C$ 90%RH, therefore the OPP film was more effective than LDPE film on the resistance of relative humidity. And the OPP film packaging sealed mixture of food samples was also more elective then LDPE film, having same thickness for increase of water vapor permeability during storage at $40^{\cire}C$. A general increase in sorption rate was found more in SPI than CS in the packaged mixtures.

• The Korea Journal of Herbology
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• v.35 no.1
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• pp.57-68
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• 2020

Objective : The aim of present study was to clarify the effect of Areca Semen and Toosendan Fructus Mixture (AT-mix) on chronic reflux esophagitis (CRE) in rats. Methods : The antioxidant activity of AT-mix was measured through DPPH and ABTS radical scavenging activities in vitro. CRE was induced in SD rats (5 weeks, male) by ligating the border forestomach and granular portion with 2-0 silk and the duodenum near the pyloric portion was covered with 2-mm wide piece of 18-Fr Nélaton catheter. And then rats were treated AT-mix 200 mg/kg one daily for 14 days. The anti-oxidant and inflammatory protein levels were evaluated using western blotting. Results : Gross lesion of esophageal mucosa after AT-mix treatment showed a superior enhancement compared with that of CRE control rats. AT-mix treatment strongly reduced both DPPH and ABTS radical scavenging activities (DPPH, IC₅₀ 8.15±0.14 ㎍/mL; ABTS, IC₅₀ 24.69±0.03 ㎍/mL, repspectively). Levels of the NADPH oxidase subunit including NOX4 and p22^phox increased in CRE control rats. Otherwise, AT-mix treatment significantly reduced. The activation of Nuclear factor-erythroid 2-related factor 2 (Nrf2) led to significantly the up-regulation of HO-1. The inhibition of IκBα phosphorylation led to NF-κB inactivation. Subsequently, NF-κB inactivation significantly induced the decrease of COX-2, iNOS, TNF-α, and IL-6 protein expressions. Conclusion : Taken together, these results suggest that AT-mix treatment can attenuate the esophageal mucosal ulcer though inhibiting NF-κB pathway and enhancing Nrf2/HO-1 pathway. Thus, the additional mechanism study about AT-mix would need for the development as a safe herbal therapy for CRE.