Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Influence of Dietary Supplementation of Condensed Tannins through Leaf Meal Mixture on Intake, Nutrient Utilization and Performance of Haemonchus contortus Infected Sheep

  • Pathak, A.K. (Centre of Advanced Faculty Training in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Dutta, Narayan (Centre of Advanced Faculty Training in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Banerjee, P.S. (Centre of Advanced Faculty Training in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Pattanaik, A.K. (Centre of Advanced Faculty Training in Animal Nutrition, Indian Veterinary Research Institute) ;
  • Sharma, K. (ICAR)
  • Received : 2013.01.24
  • Accepted : 2013.05.17
  • Published : 2013.10.01
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Abstract

The study assessed the effect of dietary supplementation of leaf meal mixture (LMM) containing condensed tannins (CT) on feed intake, nutrient utilization and performance of sheep infected with Haemonchus contortus. Eighteen adult sheep of similar age and body weight ($25.03{\pm}1.52$) were included in this study and out of these, 12 sheep were infected with single dose of infective third stage larvae of H. contortus at 2,000 larvae per sheep. The experimental sheep were allocated in three different groups' i.e. negative control (NC; no infection), control (C; H. contortus infected) and treatment (T; H. contortus infected+CT at 1.5% of the DM through LMM) and the experiment was conducted for a period of 90 d. The intake of dry matter (DM), organic matter (OM) and digestibility of DM, OM, neutral detergent fibre (NDF) and acid detergent fibre (ADF) were comparable among three animal groups. However, digestibility of crude protein (CP) and ether extract (EE) were significantly (p<0.05) higher in NC group as compared to both C and T groups. Nitrogen (N) retention (g/d or % of N intake) was significantly (p = 0.038) lower in C group as compared to T and NC groups. Daily intake (g/kg $W^{0.75}$) of digestible crude protein (DCP), digestible organic matter (DOM) and total digestible nutrient (TDN) did not differ significantly (p<0.05) in the three groups. Haemoglobin (Hb) and packed cell volume (PCV) were significantly (p<0.001) higher in treatment group as compared to control. The level of Hb and PCV reduced (p<0.001) after 30 days of experimental feeding. CT significantly (p<0.001) reduced serum urea in T group as compared to NC and C groups. Serum proteins differed significantly (p<0.01) among the three groups. The activity of serum enzymes AST, ALT, ALP and LDH were also statistically non significant (p<0.05) among treatments. The weight of abomasal lymph nodes (ALN) in T group was higher (p<0.05) than in C group. Treatment group had lower (p<0.05) total worms and fecal egg count compared to control group. It may be concluded that dietary supplementation of CT through LMM significantly improved the N retention, and inhibited the different developmental stages of Haemonchus contortus in experimental sheep.

Keywords

References

  1. Abdel Ali, T. S. 1992. Haematological and biochemical studies on efficiency of synthetic drugs against gastrointestinal nematode parasites in sheep. Aus. Vet. J. Med. 42:197-203.
  2. Ahmad, A., C. Anwar-ul-Hassan, A. Anwar-ul-Haq, and M. A. Majeed. 1990. Serum proteinogram of lambs experimentally induced Haemonchus contortus infection. Vet. Arh. 60:195-200.
  3. Ahmad, M. and J. A. Ansari. 1989. Effect of Haemonchosis on haematology and non specific phosphomonoesterase activities in sheep and goats. Helminthol. 26:295-302.
  4. Anbarasu, C., Narayan Dutta, K. Sharma, and M. Rawat. 2004. Response of goats to partial replacement of dietary protein by a leaf meal mixture containing Leucaena leucocephala, Morus alba and Tectona grandis. Small Rum. Res. 51:47-56. https://doi.org/10.1016/S0921-4488(03)00203-7
  5. Anonymous. 1984. Manual of veterinary investigation. Vol. 2 reference book 390. Ministry of Agriculture, Fisheries and Food. Her Majestry- stationary office, London, pp. 161-187.
  6. AOAC. 2000. Official methods of analysis, Association of Official Analytical Chemists, 16th edn, Washington, DC, USA.
  7. Athanasiadou, S., I. Kyriazakis, F. Jackson, and R. L. Coop. 2001. Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: in vitro and in vivo studies. Vet. Parasitol. 99:205-219. https://doi.org/10.1016/S0304-4017(01)00467-8
  8. Balic, A., V. M. Bowles, and E. N. Meeusen. 2000. Cellular profiles in the abomasal mucosa and lymph node during primary infection with Haemonchus contortus in sheep. Vet. Immunol. Immunopathol. 75: 109-120. https://doi.org/10.1016/S0165-2427(00)00189-6
  9. Barham, D. and P. Trinder. 1972. An improved color reagent for the determination of blood glucose by the oxidase system. Analyst 97:142-145. https://doi.org/10.1039/an9729700142
  10. Bhatta, R., A. K. Shinde, S. Vaithiyanathan, S. K. Sankhyan, and D. L. Verma. 2002. Effect of polyethylene glycol-6000 on nutrient intake, digestion and growth of kids browsing Prosopis cineraria. Anim. Feed Sci. Technol. 101:45-54. https://doi.org/10.1016/S0377-8401(02)00180-3
  11. Boyd, J. W. 1984. The interpretation of serum biochemistry test results in domestic animals. Vet. Clin. Pathol. 13:7-14.
  12. Bretaudiere, J. P., A. Vassault, L. Amsellem, M. L. Pourci, H. Thiece-Phung, and M. Bailly. 1977. Criteria for establishing a standardized method for determining alkaline phosphatase activity in human serum. Clin. Chem. 23:2263-2274.
  13. Buddle, B. M., G. Jowett, R. S. Green, P. G. Douch, and P. L. Risdon. 1992. Association of blood eosinophilia with the expression of resistance in Romney lambs to nematodes. Int. J. Parasitol. 22: 955-960. https://doi.org/10.1016/0020-7519(92)90053-N
  14. Cenci, F. B., H. Louvandini, C. M. McManus, A. Dell'Porto, D. M. Costa, S. C. Araujo, A. P. Minho, and A. L. Abdalla. 2007. Effects of condensed tannin from Acacia mearnsii on sheep infected naturally with gastrointestinal helminthes. Vet. Parasitol. 144:132-137. https://doi.org/10.1016/j.vetpar.2006.09.021
  15. Chaichisemsari, M., B. Eshratkhah, N. Maherisis, M. Sadaghian, and S. Hassanpour. 2011. Evaluation of total protein, albumin, globulin and blood urea nitrogen concentrations in gastrointestinal nematodes infected sheep. Global Vet. 6:433-437.
  16. Chandrawathani, P., M. Adnan, and C. M. Zaini. 2000. Preliminary study on Neem (Azadirachta indica) as an alternative anthelmintic for sheep. Proc. 12th Vet. Assoc. Malaysia Scientific Cong., Malaysia, Kuanyan.
  17. Coop, R. L. 1982. The impact of sub clinical parasitism in ruminants. In: Parasites-Their world and Ours (Ed. D. F. Mettrick and S. S. Desser) Amsterdam: Elsevier, pp. 439-450.
  18. Dacie, Z. and S. Lewis. 1969. Practical Heamatology 4th ed. J. and A., Churchill, London.
  19. Dawkins, H. J. S., R. G. Windon, and G. K. Eagleson. 1989. Eosinophil responses in sheep selected for high and low responsiveness to Trichostrongylus colubriformis. Int. J. Parasitol.19:199-205. https://doi.org/10.1016/0020-7519(89)90008-8
  20. Dey, A., N. Dutta, K. Sharma and A.K. Pattanaik. 2008. Effect of dietary inclusion of Ficus infectoria leaves as a protectant of proteins on the performance of lambs. Small Rumin. Res. 75: 105-114. https://doi.org/10.1016/j.smallrumres.2007.06.013
  21. Diogenes, P. V. A., A. C. D. Suassuna, S. M. M. Ahid and B. Soto-Blanco. 2010. Serum protein electrophoretic profile of goats infected with Haemonchus contortus. J. Anim. Vet. 9:1603-1606. https://doi.org/10.3923/javaa.2010.1603.1606
  22. Doumas, B. T., W. A. Watson, and H. G. Biggs. 1971. Albumin standards and the measurements of serum albumin with bromocresol green. Clin. Chem. Acta. 31:87-96. https://doi.org/10.1016/0009-8981(71)90365-2
  23. Dubey, M., N. Dutta, P. S. Banerjee, A. K. Pattanaik, K. Sharma, and M. Singh. 2012. Effect of condensed tannin supplementation through a tree leaves mixture on erythrocytic antioxidant status and gastrointestinal nematodes in kids. Anim. Nutr. Feed Technol. 12:91-102.
  24. Entrocasso, C. M., J. J. Parkins, J. Armour, K. Bairden, and P. N. McWilliam. 1986. Metabolism and growth studies in housed calves given a morantel sustained release bolus and exposed to natural trichostrongyle infection. Res. Vet. Sci. 40:65-75.
  25. Etter, E., H. Hoste, C. Chatier, I. Pors, C. Koch, C. Broqua, and H. Coutineau. 2000. The effect of two levels of dietary protein on resistance and resilience of dairy goats experimentally infected with Trichostrongylus colubriformis: comparison between high and low producers. Vet. Res. 31:247-258. https://doi.org/10.1051/vetres:2000120
  26. Fox, M. T., D. Gerelli, S. R. Pett, D. E. Jacob, M. Gill, and D. L. Gale. 1989. Ostertagia ostertagi infection in the calf: effects of a trickle challenge on appatite, digestibility, rate of passage of digesta and live weight gain. Res. Vet. Sci. 47:294-298.
  27. Ganai, A. M., H. A. Ahmad, and S. Bilal. 2010. Nutritional evaluation of green mulberry (Morus multicaulis) leaves in sheep. Anim. Nutr. Feed Technol. 10: 133-138.
  28. Ganai, A. M., H. A. Ahmad, J. I. A. Bhat, and S. Bilal. 2007. Nutritional evaluation of green popular (Populus deltoids) foliage in sheep. Appl. Biol. Res. 9:34-38.
  29. Hallett, C. J. and J.G. H. Cook. 1971. Produced nicotinamide adenine dinucleotide- coupled reaction for emergency blood urea estimation. Clin. Chem. Acta. 35:33-37. https://doi.org/10.1016/0009-8981(71)90289-0
  30. Hassanpour, S., B. Eshratkhah, M. Sadaghian, N. Maherisis, M. Chaichisemsari, and M. kiyaninahand. 2011. Relationship between gastrointestinal parasite infection and faecal protein excretion in Moghani ewes. Int. J. Anim.Vet. Adv. 3:167-169.
  31. Hoskin, S. O., P. R. Wilson, T. N. Barry, W. A. G. Charleston, and G. C. Waghorn. 2000. Effect of forage legumes containing condensed tannins on lungworm (Dictyocaulus sp.) and gastrointestinal parasitism in young red deer (Cervus elaphus). Res. Vet. Sci. 68:223-230. https://doi.org/10.1053/rvsc.1999.0366
  32. IFOM. 2006. Proceedings of the first international conference of animal in organic production, Minnesota University, St. Paul, Minnesota, USA August, 23-25.
  33. Kaneko, J. J. 1997. Clinical biochemistry of domestic animals. 5th edn, (Academic Press, New York, USA), pp. 885-905.
  34. Kantwa, L. C., L. Gupta, B. P. Singh, and S. P. Tailor. 2006. Nutritional evaluation of mulberry leaves (Morus alba) in sheep and goat. Indian J. Small Rum. 12:202-205.
  35. Kearl, L. C. 1982. Nutrient requirements of ruminants in developing countries. International Feed Stuffs Institute, Utah Agricultural Experimental Station, (Utah State University, Logan, Utah- 84322, USA), pp. 45-81.
  36. Kyriazakis, I., D. H. Anderson, R. L. Coop, and F. Jackson. 1996. The pathophysiology and development of immunity during long-term subclinical infection with Trichostrongylus colubriformis of sheep receiving different nutritional treatments. Vet. Parasitol. 65:41-54. https://doi.org/10.1016/0304-4017(96)00947-8
  37. Kyriazakis, I., J. D. Oldham, R. L. Coop, and F. Jackson. 1994. The effect of subclinical intestinal nematode infection on the diet selection of growing sheep. Br. J. Nutr. 72:665-677. https://doi.org/10.1079/BJN19940070
  38. Leng, R. A. 1990. Factors affecting the utilization of poor quality forages by ruminants particularly under tropical condition. Nutr. Res. Rev. 3:277-303. https://doi.org/10.1079/NRR19900016
  39. MacKinnon, K. M. 2007. Differential gene expression and immune regulatory mechanism in parasitic-resisitant hair and susceptible wool sheep infected with the parasitic nematode, Haemonchus contortus. Ph D. Thesis. Faculty of Virginia Polytechnic Institute and State University, Blackberg, Virginia.
  40. MacRae, J. C. 1993. Metabolic consequence of intestinal parasitism. Proc. Nutr. Soc. 52:121-130. https://doi.org/10.1079/PNS19930044
  41. Makkar, H. P. S. 2000. Quantification of Tannins in Tree Foliage - A Laboratory Manual (FAO/IAEA Working Document, Vienna, Austria).
  42. McDonald, P., R. A. Edwards, J. F. D. Greenhalgh, and C. A. Morgan. 1995. In: Animal Nutrition 5th edn, (Longman Scientific and Technical Inc., New York).
  43. Min, B. R. and S. P. Hart. 2003. Tannins for suppression of internal parasites. J. Anim. Sci. 81:E102-E109.
  44. Misra, S. C., D. N. Panda, and S. Psida. 1996. Haematological and histological alterations of immature Paramphistomiasis in lambs. Indian Vet. J. 73:1274-1276.
  45. Molan, A. L., G. C. Waghorn, and W. C. McNabb. 1999. Condensed tannins and gastrointestinal nematodes in sheep. Proc. NZ. Grassl. Assoc. 61:57-61.
  46. Molan, A. L., G. C. Waghorn, and W. C. McNabb. 2002.Effect of condensed tannins on egg hatching and larval development of Trichostrongylus colubriformis in vitro. Vet. Rec. 150:65-69. https://doi.org/10.1136/vr.150.3.65
  47. Moore, D. A., T. H. Terrill, B. Kouakou, S. A. Shaik, J. A. Mosjidis, J. E. Miller, M. Vanguru, G. Kannan, and J. M. Burke. 2008. The effects of feeding sericea lespedeza hay on growth rate of goats naturally infected with gastrointestinal nematodes. J. Anim. Sci. 86:2328-2337. https://doi.org/10.2527/jas.2007-0411
  48. Murata, H., N. Shimada, and M. Yoshika. 2004. Current research on acute phase proteins in veterinary diagnosis: An overview. Vet. J. 168:28-40. https://doi.org/10.1016/S1090-0233(03)00119-9
  49. Newman, D. J. and C. P. Price. 1999. Renal function and nitrogen metabolites. In: Tietz Textbook of Clinical Chemistry (Ed. C. A. Burtis and E. R. Ashwood), 3rd edn, Philadelphia: W B Saunders Company, pp. 1204.
  50. Niezen, J. H., G. C. Waghorn, and W. A. G. Charleston. 1998a. Establishment and fecundity of Ostertagia circumcincta and Trichostrongylus colubriformis in lambs fed Lotus (L. pedunculatus) or perennial ryegrass (Lolium perenne). Vet. Parasitol. 78:13-21. https://doi.org/10.1016/S0304-4017(98)00121-6
  51. Niezen, J. H., H. A. Robertson, G. C. Waghorn, and W. A. G. Charleston. 1998b. Production, faecal egg counts and worm burdens of ewe lambs which grazed six contrasting forages. Vet. Parasitol. 80:15-27. https://doi.org/10.1016/S0304-4017(98)00202-7
  52. Pandey, V. S., W. Pralomkram, S. Kochapakdee, and S. Saithanoo. 2001. Benzimidazol resistance in Haemonchus contortus from goat in Thailand. PSU Goat Res. Pub. pp. 337.
  53. Paolini, V., A. Frayssines, F. D. L. Farge, P. Dorchies, and H. Hoste. 2003a. Effects of condensed tannins on established populations and on incoming larvae of Trichostrongylus colubriformis and Teladorsagia circumcincta in goats. Vet. Res. 34:331-339. https://doi.org/10.1051/vetres:2003008
  54. Paolini, V., F. De La Farge, F. Prevot, P. Dorchies, and H. Hoste. 2005. Effects of the repeated distribution of sainfoin hay on the resistance and the resilience of goats naturally infected with gastrointestinal nematodes. Vet. Parasitol. 127:277-283. https://doi.org/10.1016/j.vetpar.2004.10.015
  55. Paolini, V., J. P. Bergeaud, C. Grisez, F. Prevot, P. Dorchies, and H. Hoste. 2003b. Effects of condensed tannins on goats experimentally infected with Haemonchus contortus. Vet. Parasitol. 113:253-261. https://doi.org/10.1016/S0304-4017(03)00064-5
  56. Parkins, J. J. and P. Holmes. 1989. Effects of gastrointestinal helminth parasites on ruminant nutrition. Nutr. Res. Rev. 2: 227-246. https://doi.org/10.1079/NRR19890016
  57. Pathak, A. K. 2011. Nutritional status and performance of ruminants as influenced by gastrointestinal nematodes: An overview. NE Vet. 11:20-26.
  58. Pathak, A. K. and S. P. Tiwari. 2012a. Influence of Haemonchus contortus infection on nutrient intake and its utilization in kids fed different levels of nutrition. Indian J. Anim. Nutr. 29:52-57.
  59. Pathak, A. K. and S. P. Tiwari. 2012b. Influence of Haemonchus contortus on biochemical profile in kids fed on different diets. Vet. Pract. 13:63-65.
  60. Pathak, A. K. and S. P. Tiwari. 2013. Effect of high plane of nutrition on the performance of Haemonchus contortus infected kids. Vet. World. 6:22-26.
  61. Pathak, A. K. and S. Pal. 2008. Seasonal prevalence of gastrointestinal parasites in goats from durg district of Chhattisgarh. Vet. World 1:136-137.
  62. Patra, A. K., K. Sharma, N. Dutta, and A. K. Pattanaik. 2006. Effect of partial replacement of dietary protein by a leaf meal mixture on nutrient utilization by goats in pre and late gestation. Small Rumin. Res. 63:66-74. https://doi.org/10.1016/j.smallrumres.2005.02.008
  63. Patterson, D. M., F. Jackson, J. F. Huntley, L. M. Stevenson, D. G. Jones, E. Jackson, and J. F. Russel. 1996a. Studies on caprine responsiveness to Nematodiasis: segregation of male goats into responders and non-responders. Int. J. Parasitol. 26:187-194. https://doi.org/10.1016/0020-7519(95)00121-2
  64. Patterson, D. M., F. Jackson, J. F. Huntley, L. M. Stevenson, D. G. Jones, E. Jackson, and J. F. Russel. 1996a. The response of breeding doses to Nematodiasis: segregation into responders and non-responders. Int. J. Parasitol. 26:1295-1303. https://doi.org/10.1016/S0020-7519(96)00125-7
  65. Payne, J. M. and S. Payne. 1987. The metabolic profile test. Oxford University Press, Oxford (1987) p. 179.
  66. Richmond, W. 1973. Cholesterol: oxygen oxidoreductase enzyme catalyzes oxidation of cholesterol to 4-cholesten-3-one with production of hydrogen peroxides and $H_{2}O_{2}$ react with PAP reagent. Clin. Chem. 19:1350.
  67. Rowe, J. B., J. V. Nolan, G. de Chaneet, E. Teleni, and P. H. Holmes. 1988. The effect of haemonchosis and blood loss into the abomasum on digestion in sheep. Br. J. Nutr. 59:125-139. https://doi.org/10.1079/BJN19880016
  68. Rumosa, Gwaze, F., M. Chimonyo, and K. Dzama. 2010. Relationship between nutritionally-related blood metabolites and gastrointestinal parasites in Nguni goats of South Africa. Asian-Aust. J. Anim. Sci. 23:1190-1197. https://doi.org/10.5713/ajas.2010.90547
  69. Sastary, G. A. 1989. Veterinary clinical pathology. CBS Publishers and Distributors Pvt. Ltd., Delhi, India
  70. Scalbert, A. 1991. Antimicrobial properties of tannin. Phytochemistry 30:3875-3883. https://doi.org/10.1016/0031-9422(91)83426-L
  71. Scharenberg, A., F. Heckendorn, Y. Arrigo, H. Hertzberg, A. Gutzwiller, H. D. Hess, M. Kreuzer, and F. Dohme. 2008. Nitrogen and mineral balance of lambs artificially infected with Haemonchus contortus and fed tanniferous sainfoin (Onobrychis vicifolia). J. Anim. Sci. 86:1879-1890. https://doi.org/10.2527/jas.2007-0448
  72. Scharenberg, A., Y. Arrigo, A. Gutzwiller, U. Wyss, H. D. Hess, M. Kreuzer, and F. Dohme. 2007. Effect of feeding dehydrated and ensiled tanniferous sainfoin (Onobrychis vicifolia) on nitrogen and mineral digestion and metabolism of lambs. Arch. Anim. Nutr. 611:390-405.
  73. Shaik, S. A., T. H. Terrill, J. E. Miller, B. Kouakou, G. Kannan, R. M. Kaplan, J. M. Burke, and J. A. Mosjidis. 2006. Sericea lespedeza hay as natural deworming agent against gastrointestinal nematode infection in goats. Vet. Parasitol. 139:150-157. https://doi.org/10.1016/j.vetpar.2006.02.020
  74. Sharma, D. K., P. P. S. Chauhan, and R. D. Agarwal. 2001. Changes in the levels of serum enzymes and total protein during experimental haemonchosis in Barbari goats. Small Rumin. Res. 42:119-123. https://doi.org/10.1016/S0921-4488(01)00241-3
  75. Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods, 8th edn. East West Press Pvt. Ltd., New Delhi.
  76. Symons, L. E. A. 1982. Protein synthesis in the whole body, liver and skeletal muscle. In Parasites-Their world and Ours, pp. 233-241 (Ed. D. F. Mettrick and S. S. Desser) Amsterdam, Elsevier.
  77. Talpatra, S. K., S. C. Ray, and K. C. Sen. 1940. Estimation of phosphorus, chlorine, calcium, magnesium, sodium and potassium in foodstuffs. Indian J. Vet. Sci. Anim. Husb. 10: 243-258.
  78. Terrill, T. H., G. B. Douglas, A. G. Foote, R. W. Purchas, G. F. Wilson, and T. N. Barry. 1992. Effect of condensed tannins upon body growth, wool growth and rumen metabolism in sheep grazing sulla (Hedysarum cornarium) and perennial pasture. J. Agric. Sci. Camb. 119:265-273. https://doi.org/10.1017/S0021859600014192
  79. Thefeld, W., H. Hoffmeister, E. W. Busch, P. U. Koller, and J. Vollmar. 1974. Referenzwerte fur die Bestimmungen der Transaminasen GOT und GPT sowie der alkalischen phosphatase im serum mit optimierten standardmethoden. Dtsch. Med. Wochenschr. 99:343-351. https://doi.org/10.1055/s-0028-1107760
  80. Urquhart, G. M., J. Armour, J. L. Duncan, A. M. Dunn, and F. W. Jennings. 1996. Veterinary parasitology, 2nd edn. (Blackwell Science Ltd) pp. 276-278.
  81. Van Houtert, M. F. J. and A. R. Sykes 1996. Implications of nutrition for the ability of ruminants to withstand gastrointestinal nematode infections. Int. J. Parasitol. 26:1151-1167. https://doi.org/10.1016/S0020-7519(96)00120-8
  82. Van Soest, P. J., J. B. Robertson, and B. A. Lewis. 1991. Methods for dietary fibre, neutral detergent fibre and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74: 3583-3597. https://doi.org/10.3168/jds.S0022-0302(91)78551-2
  83. Vatzidis, H. 1977. An improved biuret reagent. Clin. Chem. 23: 908.
  84. Waghorn, G. C. 1996. Condensed tannins and nutritive absorption from small intestine. Proc. Can. Soc. Anim. Sci., Lethbridge, Alberta, Canada. L. M. Rode, edn.
  85. Waghorn, G. C., I. D. Shelton, and B. R. Sinclair. 1990. Distribution of elements between solid and supernatant fractions of digesta in sheep given six diets. NZ. J. Agric. Res. 33:259-269. https://doi.org/10.1080/00288233.1990.10428418
  86. Wroblewski, V. F. and L. S. Duean. 1955. Bestimmung der Aktivital der lactate dehydragenase. Proc. Soc. Exp. Biol. 90: 210-214. https://doi.org/10.3181/00379727-90-21985
  87. Yanez-Ruiz, D. R. and E. Molina-Alcaide. 2008. A comparative study of nutrients utilization, alkaline phosphatase activity and creatinine concentration in the serum of sheep and goats fed diets based on olive leaves. J. Anim. Physiol. Anim. Nutr. 92: 141-148. https://doi.org/10.1111/j.1439-0396.2007.00719.x

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