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Antioxidative Effect of Plant Food Mixtures in Rat Fed on High Fat-High Cholesterol Diet  

Kwak, Chung-Shil (Aging and Physical Culture Research Institute, Medical college of Seoul National University)
Kim, Mee-Yeon (Department of Food and Nutrition, Ewha Woman's University)
Lee, Mee-Sook (Department of Food and Nutrition, Hannam University)
Publication Information
Journal of Nutrition and Health / v.38, no.5, 2005 , pp. 352-363 More about this Journal
Abstract
The previous extensive in vitro studies on the antioxidative activities of a number of Korean grains, vegetables, seaweeds and mushrooms, and the various combinations of these food source exhibited a wide range of antioxidative activities, and four food mixtures composed of 5 kinds of foods (5A, 5B, 5C and 5D) were designed from 16 selective foods showing. high antioxidant effect, in vitro, to find the good combinations for the meal planning. Mixture 5B or 5C contained very high levels of total flavonoid and polyphenol, and ethanol extract from 5A, 5B or 5C showed very strong inhibitory effects against in vitro $Fe^{2+}-induced$ lipid peroxidation and ethanol extract from 5B or 5C showed remarkable DPPH radical scavenging effect and lipid peroxide-protein conjugation inhibition effect. And in vivo study was also carried out with two mixtures (5B, 5C). Powders (P5B, P5C) or ethanol extracts (E5B, E5C) of these mixtures were supplemented to Sprague-Dawley rats fed on high fat $(15\%)-high$ cholesterol $(1\%)$ semipurified diet for 5 weeks. The total antioxidant power in serum was significantly higher in P5B, P5C, E5B and E5C groups than in high fat control group, and $ascorbate-Fe^{2+}-induced$ TBARS was significantly lowered by E5B supplementation in rat liver. In liver tissue, Cu, Zn-SOD activity was significantly higher in P5B and E5B groups than in high fat control group, while catalase or GSH-peroxidase (GPx) activity was not changed by any supplementations. In kidney, Cu, Zn­SOD activity was significantly higher in P5B group than in high fat control group, while GPx activity was not changed by any supplementations. Taken together, mixture 5B and 5C showed very strong antioxidative effects both in vitro and in vivo. Therefore, the ingredient Korean foods of 5B and 5C could be recommended to take a lot together for prevention from age-related chronic diseases.
Keywords
food mixture; ethanol extract; total antioxidant power; ascorbate-induced TBARS; lipid peroxide­protein conjugation.;
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1 Sohal RS, Sohal BH, Brunk UT. Relationship between antioxidant defenses and longevity in different mammalian species. Mech Aging Dev 53: 217-227. 1990   DOI   ScienceOn
2 Cutler RG. Antioxidants and aging. Am J Clin Nutr 53 (IsuppI) : 373s-379s, 1991
3 Jelodar GA, Kaleki M, Motadayen MR, Sims S. Effect of fenugreek, onion and garlic on blood glucoses and histopathology of pancreas of alloxan-induced diabetic rats. Indian J Med Sci 59 (2) : 64-69, 2005   DOI   ScienceOn
4 Miller NJ, Rice-Evans CA. Antioxidant activity of resveratrol in red wine. Clin Chern 41: 1789, 1995
5 Lee JS, Chang JS, Lee JY, Kim JA. Capsaicin-induced apoptosis and reduced release of reactive oxygen species in MBT-2 murine bladder tumor cells. Arch Pharm Res 27 (II): 1147-1153,2004   DOI   ScienceOn
6 Shoo HJ. The antibacterial action of garlic, onion, ginger and red pepper juice. J Korean Soc Food Sci Nutr 28(1): 94-99, 1999
7 Kang JO. Antioxidative activity of Mugwort extract on human low density lipoprotein. Kor J Soc Food Sci 16 (6): 623-628, 2000
8 Kim ES, Kim MK. Effect of dried leaf powders and ethanol extracts of Persimon, Green tea and Pine needle on lipid metabolism and antioxidative capacity in rats. Korean J Nutr 32(4): 337-352, 1999
9 Kim HJ, Lee IS, Lee KR. Antimutagenic and anticancer effects of Ramaria botrytis Rick extracts. J Korean Soc Food Sci Nutr 28 (6): 1321-1325,1999
10 Park YR. Effect of polyamine on modification of biomodics by aldehydes. Ph. D thesis. Seoul National University, 2000
11 Chae SK, Kang GS, Ma SJ, Bang KW, Oh MW, Oh SH. Standard Food Analysis. Jigu-moonwha Sa, pp. 381-382, 2002
12 Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of 'Antioxidant power': The FRAP Assay. Anal Biochem 239: 70-76, 1996   DOI   ScienceOn
13 Habig WH, Pabst MI and Jakoby WB. Glutathion S-transferase. J Bioi Chem 249: 7130-7139, 1974
14 Mattson MP. Mother's legacy: mitochondrial DNA mutations and Alzheimer's disease. Trends Neurosci 20: 373-280, 1997   DOI   PUBMED   ScienceOn
15 Terada LS, Leff A, Repine JE. Measurement of xanthine oxidase in biological tissues. In: Methods in Enzymology. vol 186, Academic Press, pp.651-652, 1990
16 RLee SJ, Choi JH. Effects of green tea catechins on the superoxide dismutase, glutathione peroxidase and xanthine oxidase activities of kidney in diabetic rats. Korean J Nutr 34(7): 734-740,2001
17 Azhar S, Cao L, Reaven E. Alteration of adrenal antioxidant defence system during aging in rats. J Clin Invest 96: 1414-1420, 1995   DOI   ScienceOn
18 Tappel AL. Lipid peroxidation damage to cell components. Fed Proc 32: 1870-1974, 1973
19 Tucker KL, Hallfrisch J, Qiao N, Muller D, Andres R, Fleg JL. The combination of high fruit and vegetable and low saturated fat intakes is more protective against mortality in aging men than is either alone: The Baltimore longitudinal study of aging. J Nutr 135: 556-561,2005
20 Lee MS, Lim SJ, Kim SA, Woo MK, Kwak CS, Park SC. Antimutagenic and antioxidative effects of ethanol extracts from Garlic, Ginger, Green onion and Red pepper. Kor J Gerontology 13 (1 ) : 8-16,2003
21 Kim YD, Yang WM. Research on component of wild plant. Korean J Food Sci Technol15 (4): 10-16,1991
22 Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 16: 144-158,1965
23 Hayashi T, Sawa K, Kawasaki M, Arisawa M, Shimisu M, Morita M. Inhibition of cow's milk xanthine oxidase by flavonoids. J Natural Products 51: 345-348, 1988   DOI   PUBMED
24 Lee E. Effects of powdered pine needle on serum and liver lipid composition and antioxidative capacity in rats fed high oxidized fat. J Kor Soc Food Sci Nutr 32 (6) : 926-930,2003
25 Jung SW, Kim MK. Effect of dried powdered of Chamomile, Sage and Green tea on antioxidative capacity in 15-month-old rats. Korean J Nutr 36(7): 699-710,2003
26 Aebi. Catalase in vitro. In: Methods in Enzymology 105: 121-126, 1984   DOI
27 Kim SK, Lee HI, Kim MK. Effect of water and ethanol extracts of persimmon leaf and green tea different conditions on lipid metabolism and antioxidative capacity in 12-month-old rats. Korean J Nutr 34 (5) : 499-512, 2001
28 Lin YL, Cheng CY, Lin YP, Lau YN, Juan IM, Lin JK. Hypolipidemic effect of green tea leaves through induction of antioxidant and phase II enzymes including superoxide dismutase, catalase and glutathione S-transferase. J Agric Food Chern 46: 1893-1899, 1998   DOI   ScienceOn
29 Kogure K, Goto S, Nishimura M, Yasumoto M, Abe K, Ohiwa C, Sassa H, Kusurni T, Terada H. Mechanism of potent antiperoxidative effect of capsaicin. Biochirn Biophys Acta 1573 (I): 84-92,2002
30 Otamari T and Sjodahl R. Increased lipid peroxidation in malignant tissues of patients with colorectal cancer. Cancer 64: 422-425, 1989   DOI   PUBMED   ScienceOn
31 Antonella S, Mario S, Maria L, Daniela M, Francesco B, and Francesco C. F1avonoids as antioxidant agents: Importance of their interaction with biomembranes. Free Rad Bio Med 19(4): 481-486, 1995   DOI   ScienceOn
32 Kang YR, Park YK, Ha TY, Moon KD. Effects of Pine needle extracts on serum and liver lipid contents in rats fed high fat diet. J Korean Socfood Nutr 25 (3): 367-373, 1996
33 Lee KI, Rhee SK, Park KY, Kim JO. Antimutagenic compounds identified from perilla leaf. Korean J Soc Food Nutr 21: 302-307, 1992
34 Rifici VA, Schneider SH, Khachadurian AK. Lipoprotein oxidation mediated by 1774 murine macrophage is inhibited by individual red wine polyphenols but not by ethanol. J Nutr 132: 2532-2537,2002
35 Im KJ, Lee SK, Park OK, Rhee MS, Lee JK. Inhibitory effects of garlic extracts on the nitrosation. J Korean Soc Agric Chern Biotechal 43 (2): 110-115, 2000
36 Jo HJ, Choi MJ. Effect of 1% garlic powder on serum and liver lipid and plasma amino acid concentration in rats fed cholesterol diet.J Korean Soc Food Sci Nutr 31 (I): 98-103,2002
37 Hyun KW, Kim JH, Song KJ, Lee JB, Jang JH, Kim YS, Lee JS. Physiological functionality in Geumsan perilla leaves from greenhouse and field cultivation. Korean J Food Sci Technol 35 (5): 975-979,2003
38 Halvorsen BL, Holte K, Myhrstad MCW, Barikmo I, Hvattum E, Remberg SF, Wold AB, Haffner K, Baugerod, Anderson LF, Moskang JO, Jacobs DR, Blomhoff RB. A systematic screeing of total antioxidants in dietary plants.J Nutr 132: 461-471,2002
39 Ninfali P, Mea G, Giorgini S, Rocchi M, Bacchiocca M. Antioxidant capacity of vegetables, spices and dressings relevant to nutrition. Br J Nutr 93 (2) : 257-266,2005   DOI   ScienceOn
40 Kim SA, Kim J, Kwak CS, Lee MS. Antimutagenic and cytotoxic effects of ethanol extracts from five kinds of seaweeds. J Korean Soc Food Sci Nutr 34(4): 451-458,2005   DOI
41 Cho SY, Han YB, Shin KU. Screening for antioxidant activity of edible plants.J Korean Soc Food Sci Nutr 30(1): 133-137,2001
42 Visioli F, Bogani P, Grande S, Galli C. Mediterranean food and health: Building human evidence. J Physiol Pharmacol 56 (s1) : 37-49,2005
43 Bae JT, Chang JS, Lee KR. Effect of Saecodon asparatus extract on expression of cell cycle-associated proteins in HepG2 cells. J Korean Soc Food Sci Nutr 31 (2) : 329-332,2002
44 Oh SI, Lee MS. Screening for antioxidative and antimutagenic capacities in 7 common vegetables taken by Korean. J Korean Soc Food Sci Nutr 32 (8): 1344-1350,2003
45 Misra HP and Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and simple assay for superoxide dismutase. J Bioi Chem 247: 3170-3175, 1972
46 Ra KS, Chung SH, Sub HJ, Son JY, Lee HK. Inhibitor of xanthine oxidase from onion skin. Korean J Food Sci Technol 30 (3) : 697-701,1998
47 Gomez-Sanchez A, Hermonsin I, Mayo I. Cleavage and oligomerization of malondialdehyde under physiological conditions. Tetrahedron Letters 28: 4077-4080, 1990
48 Surb YJ, Hurb YJ, Kang JY, Lee E, Kong G, Lee SJ. Resveratrol, an antioxidant present in red wine, induces apopotosis in human promyelocyte leukemia (HL-60) cells. Cancer Letters 140: 110, 1999
49 Report on 2001 National Health and Nutrition Survey. Ministry of Health and Welfare, 2002
50 Park JC, Ha JO, Park KY. Antimutagenic effect of flavonoids isolated from Oenanthe javanica. J Korean Soc Food Sci Nutr 25: 588-592, 1996
51 Kwak CS, Lim SJ, Kim SA, Park SC, Lee MS. Antioxidative and antimutagenic effects of Korean Buckwheat, Sorghum, Millet and Job's tears. J Korean Soc Food Sci Nutr 33(6): 921-929, 2004   DOI
52 Gotteried H and Dunkley WL. Ascorbic acid and copper in linoleate oxidation: Measurement of oxidation by ultraviolet spectrophotometry and the thiobarbituric acid test. J Lipid Res 10: 555-560, 1969
53 Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72: 248-254, 1976   DOI   PUBMED   ScienceOn
54 Block G, Patterson B, Subar A. Fruit, vegetables and cancer prevention: A review of the epidemiological evidence. Nutr Cancer 18: 1-29, 1992
55 Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Miller A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 84: 407-412, 1993
56 Frei B, Stocker R, Ames BN. Antioxidant defenses and lipid peroxidation in human plasma. Acad Aci USA 85: 9748-9752, 1988   DOI   ScienceOn
57 Hub K, Shin US, Lee SI. Effect of heat-treated ceruloplasmin on the hepatic xanthine oxidase activity and type conversion. Arch Pharm Rec 18 (1): 56-59, 1995   DOI   ScienceOn
58 Ceruti PA. Prooxidant states and tumor promotion. Science 227: 375-381, 1985   DOI   PUBMED
59 Bompart GJ, Prevot DS, Bascands JL. Rapid automated analysis of glutathione reductase, peroxidase and S-transferase activity: application to cisplatin-induced toxicity. Clin Biochem 23 (6) : 501-504, 1990   DOI   ScienceOn
60 Parfitt VJ, Rubba P, Bolton C, Marotta G, Hartog M, Mancini M. A comparison of antioxidant status and free radical peroxidation of plasma lipoproteins in healthy young persons from Naples and Bristol. Eur Heart J 15: 871-876, 1994
61 Katiyar SK, Agarwal R, Mukhtar H. Inhibition of tumor promotion in SENCAR mouse skin by ethanol extract of Zingober officinale rhizome. Cancer Res 56: 1023-1030,1996
62 Blois MS. Antioxidant detennination by the use of a stable radical. Nature 26: 1199-1200,1958
63 Miller DM, Aust SD. Studies of ascorbate-dependent, iron-catalyzed lipid peroxidation. Arch Biochem Biophys 271: 113-120, 1989   DOI   ScienceOn
64 Suzuki F, Kobayashi M, Komatsu Y, Kato A, Pollard RB. Keishika- kei-to, a traditional Chinese herbal medicine, inhibits pulmonary metastasis of B16 melanoma. Anticancer Res 17: 873-878, 1997
65 Rao CV, Rivenson A, Simi B, Reddy BS. Chemoprevention of colon carcinogenesis by dietary curcurnin, a naturally occuring plant phenolic compound. Cancer Res 55: 259-266, 1995
66 Sohn HE, Lee JY, Kim DC, Hwang WI. Enhancement of anticancer activity by combination of garlic (Allium Sativum) extract and Vitamin C. J Korean Soc Food Sci Nutr 30(2): 372-376, 2001
67 Stirpe F, Della Corte E. The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J Bioi Chem 244(14): 3855-3863, 1969
68 Tappel AL. Glutahione peroxidase and hydroperoxides. In methods in Enzymology 52: 506-513, 1978   DOI
69 Lee YC, Hwang KH, Han DH, Kim SD. Compositions of Opumtia fiscus-medica. Korean J Food Sci Technol 29 (5) : 847-853, 1997
70 Lowry OH, Rosebrough NJ, Farr AL and Rndall RI. Protein measurement with the Folin phenol reagent. J Bioi Chem 193: 265-275, 1951
71 Park CS, Kwon CJ, Choi MA, Park KS, Choi KH. Scavenging activities of mugwort and pine needle extracts. Kor J Food Preserv 9 (2) : 248-252, 2002
72 Kim HY, Lee JH, Choi JH, Kim DI, Park SH, Baik SJ, Cho WG. Effects of butanol fraction of pine needle on oxygen radical and their scavenging enzymes in liver of SD rats. Kor J Nutr 35 (3) : 292-295, 2002
73 Uesugi T, Fukui Y, Yamori Y. Beneficial effects of soybean isoflavone supplementation on bone metabolism and serum lipids in postmenopausal Japanese women: a four-week study. J Am Coll Nutr 21 (2) : 97-102,2002
74 Mo JQ, Hom DG, Anderson JK. Decreases in protective enzymes correlates with increased oxidative damage in the aging mouse brain. Mech Aging Dev 81: 73-80, 1995   DOI   PUBMED   ScienceOn
75 Park KY, Cho EJ, Rhee SH. Increased antimutagenic and anticancer activities of Chines cabbage Kimchi by changing and levels of sub-ingredient. J Korean Soc Food Sci Nutr 27 (4) : 625-632, 1998
76 Kwak HJ, Kwon YJ, Jeong PH, Kwon JH, Kim HK. Physiological activity and antioxidative effect of methanol extract from Onion (Allium cepa L). Korean J Nutr 29 (2): 349-355,2000
77 Hwang YJ, Nam HK, Chang MJ, Noh GW, Kim SH. Effect of Lentinus edodes and Pleurotus eryngii extracts on proliferation and apoptosis in human colon cancer cell lines. J Korean Soc Food Sci Nutr 32 (2) : 217-222,2003
78 Gutteridge JM. Lipid peroxidation and antioxidants as biomarker of tissue damage. Clin Chem 41 (pt 2): 1819-1826,1995