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http://dx.doi.org/10.5142/JGR.2008.32.1.039

Mechanisms of Korean red ginseng and herb extracts(KTNG0345) for anti-wrinkle activity  

So, Seung-Ho (Health Food Research Group, KT&G Central Research Institute)
Lee, Seong-Kye (Health Food Research Group, KT&G Central Research Institute)
Hwang, Eui-Il (Health Food Research Group, KT&G Central Research Institute)
Koo, Bon-Suk (Health Food Research Group, KT&G Central Research Institute)
Han, Gyeong-Ho (Health Food Research Group, KT&G Central Research Institute)
Chung, Jin-Ho (Department of Dermatology, Seoul National University College of Medicine)
Lee, Min-Jung (Department of Dermatology, Seoul National University College of Medicine)
Kim, Na-Mi (Health Food Research Group, KT&G Central Research Institute)
Publication Information
Journal of Ginseng Research / v.32, no.1, 2008 , pp. 39-47 More about this Journal
Abstract
UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. These phenomena in complex skin tissue is controlled with receptor of cell surface growth factor and cytokine receptors. The activation of receptors induces multiple downstream signaling pathways including expression of MMPs (matrix metalloproteinases). This study was aimed to elucidate the mechanism for anti-wrinkle activity of Korean red ginseng, Torilis fructus and Corni fructus mixture (KTNG0345). In this animal study, we have investigated decreasing effects of Korean red ginseng mixture on MMP-3 synthesis through diminishing $TNF-{\alpha}$ signaling that express MMP-1, -3, and -9. c-Jun and c-fos as a component of transcription factor AP-1 (activator protein-1) were analyzed the expression level using real time PCR and western blotting. c-Jun was decreased dose dependent manner both gene and protein level where as cfos was not changed. In upstream, JNK and PAK was not changed, but p38 was decreased in down stream. MMP-3, final product in this pathway was significantly decreased in dose dependent manner. These results suggest that Korean red ginseng mixture have a anti-wrinkle activity through $TNF-{\alpha}$ mediated MMPs expression pathway.
Keywords
anti-wrinkle; matrix metalloproteinase-3; AP-1; p38$TNF-{\alpha}$; Korean red ginseng;
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1 Sachsenmaier, C., Radler-Pohl, A., Zinck, R., Nordheim, A., Herrlich, P. and Rahmsdorf, H.J. : Involvement of growth factor receptors in the mammalian UVC response. Cell, 78, 963-972 (1994).   DOI   ScienceOn
2 Coffer, P.J., Burgering, B.M.T., Peppelenbosch, M.P., Bos, J.L. and Kruijer, W. : UV activation of receptor tyrosine kinase activity. Oncogene, 11, 561-569 (1995).
3 Knebel, A., Rahmsdorf, H.J., Ullrich, A. and Herrlich, P. : Dephosphorylation of receptor tyrosine kinases as target of regulation by radiation, oxidants or alkylating agents. EMBO J., 15, 5314-5325 (1996).
4 Fanger, G.R., Johnson, N.L. and Johnson, G.L. : MEK kinases are regulated by EGF and selectively interact with Rac/Cdc42. EMBO J., 16, 4961-4972 (1997).   DOI   ScienceOn
5 Choi, S. : Epidermis proliferative effect of the Panax ginseng ginsenoside Rb2. Arch. Pharm. Res., 25, 71-76 (2002).   DOI   ScienceOn
6 Kitajima, J., Suzuki, N., Ishikawa, T. and Tanaka, Y. : New hemiterpenoid pentol and monoterpenoid glycoside of Torilis japonica fruit and consideration of the origin of apiose. Chem. Pharm. Bull., 46, 1583-1586 (1998).   DOI
7 박종희 외: 상용약용식물도감, 신일상사, 196 (2000).
8 Beata Skiba, Bronwyn Neill, Terrence J. Piva.: Gene expression profiles of TNF-$\alpha$, TACE, furin, IL-1b and matrilysin in UVA and UVB-irradiated HaCat cells. Photodermatol Photoimmunol Photomed, 21, 173-182 (2005).   DOI   ScienceOn
9 Hee-Jae Cha, Moon-Taek Park, Hae-Young Chung, Nam Deuk Kim, Hiroshi Sato, Motoharu Seiki and Kyu-Won Kim. : Ursolic acid-induced down-regulation of MMP-9 gene is mediated through the nuclear translocation of glucocorticoid receptor in HT1080 human brosarcoma cells. Oncogene, 16, 771-778 (1998).   DOI
10 Chung, S., Kong, S., Seong, K. and Cho, Y.H. : $\gamma$-Linolenic acid in borage oil reverses epidermal hyperproliferation in guinea pigs. J. Nutr., 132, 3090-3097 (2002).   DOI
11 Masaki, H., Atsumi, T. and Sakurai, H. : Detection of hydrogen peroxide and hydroxyl radicals in murine skin fibroblasts under UVB irradiation. Biochem. Biophys. Res. Commun., 206, 474-479 (1995).   DOI   ScienceOn
12 Fujita, S. : Miscellaneous contributions to the essential oils of plant from various territoties. II. On the components of essential oils of Torilis japonica (Houtt.) DC. Yakugaku Zasshi, 110, 771-775 (1990).   DOI
13 Kim, B.H., Park, K.W., Kim, J.Y., Jeong, I.Y., Yang, G.H., Cho, Y.S., Yee, S.T., Seo, K.I. : Purification and characterization of anticarciongenic compound from Corni fructus. Korean J. Food Sci., 36, 1001-1007 (2004).   과학기술학회마을
14 Choi, W.Y., Chun, H.J., Lee, J.H. and Seung, H.B. : Effects of methanol extract from Cornis fructus on melanogenesis. Kor. J. Pharmacogn., 34, 70-74 (2003).   과학기술학회마을
15 이숙경 : 피부미용과 영양. 도서출판 정담 (2000).
16 Bateman, J.F., Lamand, S.R. and Ramshaw, J.A.M. : Collagen superfamily. Extracellular Matrix, Harwood, New York, 2, 22-67 (1996).
17 Miller, C.C., Tang, W., Ziboh, V.A., Fletcher, M.P. : Dietary supplementation with ethyl ester concentrates of fish oil(n- 3) and borage oil(n-6) poly unsaturated fatty acid induces epidermal generation of local putative anti- inflammatory metabolites. J. Invest. Dermatol., 96, 98-103 (1991).   DOI   ScienceOn
18 Tanno, O., Ota, Y., Kitamura, N., Katsube Tand Inoue, S. : Nicotinamide increases biosynthesis of ceramides as well as other stratum corneum lipids to improve the epidermal permeability barrier. Br. J. Derm., 143, 524-531 (2000).   DOI   ScienceOn
19 Rosette, C. and Karin, M. : Ultraviolet light and osmotic stress: activation of the JNK cascade through multiple growth factor and cytokine receptors. Science, 274, 1194- 1197 (1996).   DOI   ScienceOn
20 Dy, L.C., Pei, Y. and Travers, J.B. : Augmentation of ultraviolet B radiation- induced tumor necrosis factor production by the epidermal platelet- activating factor receptor. J. Biol. Chem., 274, 26917-26921 (1999).   DOI
21 Warmuth, I., Harth, Y.,Matsui, M.S.,Wang, N. and DeLeo, V.A. : Ultraviolet radiation induces phosphorylation of the epidermal growth factor receptor. Cancer Res., 54, 374-376 (1994).
22 Fisher, G.J. and Voorhees, J.J. : Molecular mechanisms of photoaging and its prevention by retinoic acid: ultraviolet irradiation induces MAP kinase signal transduction cascades that induce AP-1-regulated matrix metalloproteinases that degrade human skin in vivo. J. Invest. Dermatol., 3, 61- 68 (1998).
23 Lee, E.H., Cho, S.Y., Kim, S.J., Shin, E.S., Chang, H.K., Lee, T.R. : Ginsenoside F1 protects human HaCaT keratinocytes from ultraviolet-B-induced apoptosis by maintaining constant levels of Bcl-2. J. Invest. Dermatol., 121, 607-613 (2003)   DOI   ScienceOn
24 Yasui, H. and Sakurai, H. : Chemiluminescent detection and imaging of reactive oxygen species in live mouse skin exposed to UVA. Biochem. Biophys. Res. Commun., 269, 131-136 (2000).   DOI   ScienceOn
25 Yamamoto, Y. : Role of active oxygen species and antioxidants in photoaging. J. Dermatol. Sci., 27, S1-S4 (2001).
26 Fisher, G.J., Datta, S., Talwar, H.S., Wang, Z.Q., Varani, J., Kang, S. and Voorhees, J.J. : Molecular basis of sun-induced premature ageing and retinoid antagonism. Nature, 379, 335-339 (1996).   DOI   ScienceOn
27 Fisher, G.J., Talwar, H.S., Lin, J.Y., Lin, P.P., McPhillips, F.,Wang, Z.Q., Li, X.-Y.,Wan, Y.S., Kang, S. and Voorhees, J.J. : Retinoic acid inhibits induction of c-Jun protein by ultraviolet radiation that occurs subsequent to activation of mitogen-activated protein kinase pathways in human skin in vivo. J. Clin. Invest., 101, 1432-1440 (1998).   DOI   ScienceOn
28 Pawson, T. and Scott, J.D. : Signaling through scaffold, anchoring, and adaptor proteins. Science, 278, 2075-2080 (1997).   DOI   ScienceOn
29 Lee, H.J., Kim, S.R., Kin, J.S., Moon, C.J, Bae, C.S., Jang, J.S., Jo, S.K. and Kim, S.H. : The Effect of Red Ginseng on Ultraviolet B-induced Skin Damages in Mouse. J.Ginseng Res., 30, 194-198 (2006).   과학기술학회마을   DOI
30 약초의 성분과 이용, 일월서각, 535 (1999).
31 정보섭, 신민교: 도해 향약(생약)대사전, 영림사, 416 (1990).
32 Lee, E.B., Kim, S.H., Kim, T.H. : Anti-inflammatory activities of Torilis japonica fruit. Kor. J. Pharm., 29, 384-390 (1998).
33 Lee, J.H., Lee, B.S., Yang, M.S., Byun BS, Kim, W.G., Kim, B.H. and Lee, S.J. : Prevention of Photoaging and Wrinkle Formation in Hairless Mice Dorsal Skin by AP3-03. Korean J. Food Sci. Technol., 37, 986-996 (2005).
34 약용인삼, 공립출판주식회사, Japan, 268-271 (2000).
35 Kim, S.J., Kang, B.Y., Cho, S.Y., Sung, D.S., Chang, H.K., Yeom, M.H., Kim, D.H., Sim, Y.C. and Lee, Y.S. : Compound K induces expression of hyaluronan synthetase 2 gene in transformed human keratinocytes and increases hyaluronan in hairless mouse skin. Biochem. and Biophysical Research Communications, 316, 348-355 (2004).   DOI   ScienceOn
36 Barber, L.A., Spandau, D.F., Rathman, S.C., Murphy, R.C., Johnson, C.A., Kelley, S.W., Hurwitz, S.A. and Travers, J.B. : Expression of the platelet- activating receptor results in enhanced ultraviolet B radiation-induced apoptosis in a human epidermal cell line. J. Biol. Chem., 273, 18891- 18897 (1998).   DOI   ScienceOn
37 Itokawa, H., Minashi, S., Watanabe, K., Natsumoto, H. and Hamanaka, T. : Studies on the constituents of crude drugs having inhibitory activity against constraction of the ileum caused by histamine of barium chloride. Shoyakugaku Zasshi, 37, 223-228 (1983).
38 Choi, W.Y., Chun, H.J., Lee, J.H. and Seung, H.B. : Effects of methanol extract from Cornis fructus on melanogenesis. Kor. J. Pharmacogn., 34, 70-74 (2003).   과학기술학회마을
39 Kitajima, J., Suzuki, N. and Tanaka, Y. : Guaiana- type sesquiterpenoid glycosides from Torilis japonica. Chem. Pharm. Bull., 46, 1743-1741 (1998).   DOI   ScienceOn
40 Kim, Y.H. : Isolation of constituents from the fruits of Cornus officinalis Siebold. J Korean Soc Food Sci Nutr., 14, 287- 292 (1999).
41 Seung-Ho So, Seong-Kye Lee, Eui-Il Hwang, Bon-Suk Koo, Gyeong-Ho Han and Na-Mi Kim.: Effects of Korean red ginseng and herb extracts mixture(KTNG0345) on procollagen biosynthesis and matrix metalloproteinase -1 activity in human dermal fibroblast cell. J. Ginseng Res., 31(4), 196- 202 (2007)   과학기술학회마을   DOI
42 Guilian, T., Zhang, T., Yang, F., Ito, Y. : Seperation of gallic acid from Cornus Officinalis Sieb. et Zucc by high speed counter-current chromatography. J. Chromatogr. A., 886, 309-312 (2000).   DOI   ScienceOn
43 Lee, E.B., Choi, B.C., Cho, T.S. : Pharmacological studies on ether fraction of Corni Fructus. Yakhak Hoeji., 29, 1-10 (1985).
44 Min Jung Lee, Chong Hyun Won, Se Rah Lee, Ji Sook Kim, Inn Gyung Oh, Euiil Hwang, Nami Kim, Byeong Choel Kang and Jin Ho Chung. : Oral administration of Red Ginseng Extracts reduces UV-induced skin wrinkle formation in Hairless Mice. J. Ginseng Res.
45 Angel, P. and Karin, M. : The role of Jun, Fos and the AP- 1 complex in cell- proliferation and transformation. Biochim. Biophys. Acta, 1072, 129-157 (1991).
46 Chen, C.-C., Mo, F.-E. and Lau, L.F. : The angiogenic factor Cyr61 activates a genetic program for wound healing in human skin fibroblasts. J. Biol. Chem., 276, 47329-47337 (2001).   DOI   ScienceOn
47 Kang, S., Cho, S., Chung, J.H., Hammerberg, C., Fisher, G.J., Voorhees, J.J. : Inflammation and extracellular Matrix Degradation Mediated by activated transcription factors nuclear factor-kB and activator protein-l in Inflammatory acne lesions in vivo. Am J. Pathol., 166, 1691-1699 (2005).   DOI   ScienceOn
48 Brenneisen, P., Wenk, J., Klotz, L.O., Wlaschek, M., Briviba, K., Krieg, T., Sies, H. and Scharffetter-Kochanek, K. : Central role of ferrous/ferric iron in the ultraviolet B irradiationmediated signaling pathway leading to increased interstitial collagenase (matrix-degrading metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) mRNA levels in cultured human dermal fibroblasts. J. Biol. Chem., 273, 5279-5287 (1998).   DOI   ScienceOn