• Title/Summary/Keyword: protected culture

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Viability Change of Mycobacterium Tuberculosis in the Sputum Specimens Stored at Different Temperatures with or without Preservatives (객담보관온도별 결핵균의 생활력 변화와 오염방지를 위한 방부제에 관한 연구)

  • Kim, Sang-Jae;Hwang, Hae-Do
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.4
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    • pp.435-439
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    • 1986
  • The viability of tubercle bacilli in the sputum specimens has been investigated after different periods of storage at diffeent temperatures and in the presence of different preservatives. No loss of culture positives was observed for one week storage at $4^{\circ}C$, but 9.8% 19.5%C, and 26.8% of sputums failed to yield positives on 2, 3, and 4 weeks of storage respectively. At $25^{\circ}C$ even one week storage made 19.5% of sputums fail to yield positive culture and 2, 3, and 4 weeks of storage made 36.6% 70.7%, and 90.2% of sputums fail to yield positive culture respectively. And contamination was unacceptably high beyond one week of storage at $25^{\circ}C$. Contamination of sputum specimens could be protected fairly well by 0.5% boric acid, by 5% trisodium phosphate or by 0.5% cetylpyridium chloride, but, except CP, the former two had no advantage at all to protect viability of tubercle bacilli over the specimens without preservative. The CP was much less harmful to the viability of tubercle bacilli than BA, yielding 61.0% and 31.7% of culture positives on 3 and 4 weeks of storage in the presence of CP, while BA yielded 34.1% and 4.9% of positives on the same respective periods of storage. Therefore CP may be useful to preserve sputums if it takes more than 2 weeks to transport them at the temperature of over $25^{\circ}C$.

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A Study on anthropology of education of 'character' (인성과 교육의 관계적 의미 고찰: '문질빈빈' 인성 고찰을 통한 교육인류학적 함의 탐색)

  • Shin, Hyun-Seok;Kim, Sang-cheol
    • (The)Korea Educational Review
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    • v.23 no.2
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    • pp.131-155
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    • 2017
  • In this study, I examine what is called character. It is to establish what constitutes a relationship between life, culture, and education. In addition, I try to explore the possibility of anthropology of education as an academic basis for character education by using the way of '문 질빈빈(文質彬彬)'. As a result of the study, 'character' in terms of '문(culturality)' aspect implies the qualities, the nature, the disposition, and the meaning of virtue. A 'character education' can be seen in school education as a moral consideration, considering the social context, such as the ability to live harmoniously. In terms of '질(naturality)', I will look at both the innate aspect of the character and the acquired aspect through the 'character' which is the essence of character. Character is the concept of both parties, and if it is influenced by an external environment, one can seek better ways to improve the chances of improvement through education. Furthermore, the role of education is inevitably required in order to achieve the goal of 'lesser human being' to 'better human being'. Home and school education can have a positive impact on the character. An honest mind about humanity among family members is the right character. The importance of humanity is considered as a value to be recognized and protected in our society because the logic that it protects the family by character and helps to maintain the social order influences to the legal culture tradition of the modern. Therefore, the academic approach through anthropology of education has sufficient value of trial study for exploring the relationship between character, education, and culture for teachers and learners, and is appropriate for providing an academic foundation.

Analysis on Growth and Yield of Cherry Tomato Grown in a Two-Story Bed System Adapted to Strawberry Cultivation as Affected by the Planting Time during the Uncultivated Period (딸기 재배용 2단 베드 시스템에서 휴작기 이용 방울토마토 재배 시 정식 시기에 따른 생육과 생산성 분석)

  • Choi, Hyo Gil;Moon, Byoung Yong;Kang, Nam Jun;Ko, Dae Whan;Kwon, Joon Kook;Lee, Jae Han;Park, Kyoung Sub
    • Horticultural Science & Technology
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    • v.34 no.2
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    • pp.228-235
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    • 2016
  • This study was conducted to determine the yield of cherry tomato (Solanum lycopersicum Mill.) grown at three planting times during the uncultivated period of strawberry. Cherry tomato was planted under condition filled with strawberry dedicated culture medium on a two-story bed with April 20, April 30, and May 10 at 2015. Fruit harvest was completed on July 31. The supply concentration of nutrient solution at the time of transplanting was started as EC $1.2dS{\cdot}m^{-1}$ and it was gradually increased to EC $2.5dS{\cdot}m^{-1}$ after blooming of the first flower cluster. Netherlands PBG solution was supplied for one minute six times per day. The heights of cherry tomato plants planted at earlier were significantly greater than those of tomato plants planted later. The heights of cherry tomato plants grown at the bottom of the bed were greater than those grown in the upper bed. The yield of cherry tomatoes planted on April 20 at the bottom of the bed was greatest with an average of 2,954 g of tomatoes per plant. There were no significant differences in the average weight and sugar content of fruit according to planting times and bed position. The yield of cherry tomato plants planted on April 20 was 18% and 34% higher than that of plants planted on April 30 and May 10. We confirmed to increase the yield of the cherry tomato when early plants planted on two-story bed. These results indicate that farmers can choose the best period of producing cherry tomato during the un-cultivated period of strawberry under two-story bed conditions.

Effects of Transplanting and Runner Releasing Times of Mother Plants for the Control of Daughter Plant Production Time in Cutting Strawberries (딸기 삽목 시 자묘 생산시기 조절을 위한 어미묘의 정식시기 및 런너 방임시기에 따른 효과)

  • Lim, Mi Young;Jeong, Ho Jeong;Choi, Gyeong Lee;Kim, So Hui;Choi, Su Hyun
    • Journal of Bio-Environment Control
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    • v.29 no.4
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    • pp.337-343
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    • 2020
  • This study was carried out to evaluate the yield of cuttings according to the planting and runner releasing times of mother plants in order to raise the cutting seedlings of raising seedling period 75 days or more needed for forcing culture of strawberries to be transplanted into the field around the 15th of September. Three domestic cultivars of 'Maehyang', 'Jukhyang', and 'Kuemsil' were tested. For experiment 1 to determine the yield of cuttings with the change of transplanting time, the mother plant were planted on February 28, March 20, and April 9 in 20 days intervals, and the cuttings were collected two to three times from June 4 to July 1. Experiment 2 was conducted to investigate the yield of cuttings depending on the runner releasing time, the runners were released in three intervals of 20 days, 40 days, and 60 days after planting the mother plant on March 5, and the cutting were collected once to three times from May 29 to June 26. From the comparisons of cutting yield according to the transplanting time of mother plants, February 28 treatment was more 9~25% and 114~165% for each cultivar than March 20 and April 9, respectively (Experiment 1). The yield of cuttings with releasing time 20 days after planting the mother plants had higher by 60~77% and 104~176% for each cultivar than 40 days and 60 days, respectively (Experiment 2). From these results, in case of propagating the seedlings from cuttings needed for field planting around September 15, early planting around in the latter part of February is the best for cuttings yield. In addition, releasing after the removal of the runners produced from mother plants by 20 days after planting gives an advantage over higher yield of cuttings. Consequently, this study suggest to apply an efficient raising seedling system for labor saving and quality improvement in raising seedlings of three strawberry cultivars in Korea.

Effect of Concentration of Nutrient Solution on Water and Nutrient Uptake of Tomato Cultivars in Hydroponics (배양액 농도가 수경재배 토마토의 품종별 생육과 양수분 흡수특성에 미치는 영향)

  • Choi, Gyeong Lee;Yeo, Kyung Hwan;Choi, Su Hyun;Jeong, Ho Jeong;Kang, Nam Jun
    • Journal of agriculture & life science
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    • v.53 no.1
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    • pp.13-21
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    • 2019
  • This study was carried out to acquire basic data for a long-term hydroponic culture through investigating water and inorganic ion uptake characteristics at different EC level of nutrient solution of three tomato varieties. Three different tomato varieties, the European type(cv. Daphnis), the Asian type(cv. Super Doterang) and cherry type(cv. Minichal), were used for the investigation. Also, the deep flow technique(DFT) was applied. The three different electrical conductivity(EC) level(1.0, 2.0, 3.0, and 4.0 dS·m-1) of hydroponic nutrient solution were used as variable. At a high EC level of nutrient solution, the leaf area and fresh weight decreased in the early stage, and its growth(plant height, leaf number, leaf area, fresh-weight) was poor with salt stress. Result showed that the higher the EC level of the nutrient solution, the lesser was water uptake. The water uptake was not significantly different from varieties in the first survey, but In the second survey, the 'Daphnis' did not show a significant decrease in water uptake in the EC level higher than 2.0 dS·m-1., on the other hand, 'Super Doterang' presented very low water uptake. At a low EC level, N, P, and K, were absorbed more than the concentration of the irrigation water, while Ca, Mg, S uptake were low. At a high EC level, almost ions absorbed less than 50% of the initial concentration of irrigation water. Thus, imbalance among ions was severe at low EC level compared to high EC level. 'Daphnis' was a variety that effectively utilize nutrients under nutrient stress, showing high absorption at low concentration condition and low absorption at high concentration condition. However, 'Daphnis' suffered most seriously by absorbing nutrients excessively.

Effect of Environmental Conditions on Growth and Quality of Log-cultured Oak Mushroom(Lentinus edodes(Berk) Sing) under Protected Cultivation (시설내 환경조건이 원목재배 표고버섯의 생육과 품질에 미치는 영향)

  • Son, Jung-Eek;Choi, Won-Seok
    • Journal of Bio-Environment Control
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    • v.14 no.3
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    • pp.160-165
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    • 2005
  • Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequate environmental control is very essential to improve the yield and quality under protected cultivation. In this study, the diameter, thickness, length and hardness of cap and stipe, the dry weight, fresh weight and moisture content of the mushrooms were analyzed under the environmental conditions such as night air temperatures (control, $5{\~}11^{\circ}C$ and $12{\~}18^{\circ}C$), relative humidities in the daytime (control, humidifying for 6 h and 12 h), and wind velocities (control, fan-operation for 6 h and 12 h). At a lower night temperature, the quality of the mushrooms was improved, and considered to be optimum at $3{\~}8^{\circ}C$ in terms of fresh weight and quality. At $70{\~}80{\%}$ of relative humidity in the daytime, the fresh weight was increased, but the quality decreased. Considering the quality, the relative humidity in the daytime is adequate at around $50{\%}$. At 6 h of maintaining the wind velocity at $1{\~}2\;my{\cdot}s^{-1}$, the growth and quality were better, but the lightness became higher at 12 h although the fresh weight was reduced by evaporation. It is concluded that even though both of the yield and quality are not easily improved at a same time, optimum approaches should be required by using the relationship among environmental factors and the growth and quality.

Legislation of Building Outdoor Performance Hall with in Sports Park (체육공원내의 야외공연장 건립에 관한 법제(法制))

  • Lee, Sung-Ho;Kim, Mal-Ae
    • The Journal of the Korea Contents Association
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    • v.12 no.1
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    • pp.211-224
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    • 2012
  • The performance-related industry has grown independently without being protected by the nation's great policy and legal boundary in the meantime. Even in the aspect of performance Act, the thoroughly pro-regulation policy on culture & art was taken while proceeding with segmenting the legislation rather than the freedom of performance art or the promotion of performance activity. Totally 17 cases of regulations including the abolition of scenario review system in January 1999 were fully abolished. Even 6 cases of regulations were steeply eased. Also, the importance of culture & art was recognized. Thus, to promote and support it in the governmental dimension, the substantial performance art policy system was adopted for training the performance art staff manpower and the national subsidy on performance hall. In performance art, the necessity of professionals' participation was imprinted such as stage lighting, sound, and stage machine. Accordingly, many regulations on performance art were all abolished except only the minimum issues for maintaining public order in about 50 years since the establishment of the government. 'Movie' was excluded from the definition of 'public performance' in 2002. Thus, the performance report system, which had been left institutionally from the Japanese colonial period, was eternally abolished. Following this, the performance Act was changed into the legislation of the supporting promoting policy, which reflected historical situation of needing to contribute to promoting public welfare, from the regulation-centered Act.

Mycelial growth properties of an Amanita javanica strain under various culture conditions (노란달걀버섯 균주의 여러 환경 조건에 따른 균사 생장 특성)

  • Kang, Jung-A;Ka, Kang-Hyeon;Kim, Jun Young;Yoon, Seong-Tak;Kim, Seong Hwan
    • Journal of Mushroom
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    • v.17 no.4
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    • pp.191-196
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    • 2019
  • The edible ectomycorrhizal mushroom Amanita javanica is a valuable species protected by forest law in Korea. However, basic characterization data on its use as an important forest resource has been limited. This study was performed to determine mycelia growth characteristics of the domestically isolated Amanita javanica strain NIFoS 1267 on potato dextrose agar media under diverse culture conditions. Physical factors temperature, pH, and light, as well as chemical factors salts, heavy metals, and pesticides were examined for their effects on the growth of the mushroom strain. The mycelia of A. javanica strain exhibited optimal growth when cultured in dark at 30℃ in media with a pH of 5-6. Normal levels of growth were observed in media containing up to 2% saline. At a heavy metal ion content of 50 ppm, mycelial growth was not affected by arsenic ion but was affected by cadmium and lead ions. In the tests performed with two pesticides used in Korean forests, the growth of the mushroom strain was not affected by the presence of abamectin, but was inhibited in media containing acetamiprid, emamectin benzoate, or thiacloprid. These results are expected to facilitate artificial cultivation of A. javanica as a new commercial product.

Breeding of New Ever-bearing Strawberry 'Gangha' for Summer Culture (사계성 딸기 '강하' 육성)

  • Lee, Jong-Nam;Lee, Jun-Gu;Lee, Eung-Ho;Kim, Hye-Jin;Jang, Suk-Woo
    • Horticultural Science & Technology
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    • v.28 no.3
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    • pp.515-518
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    • 2010
  • 'Gangha' is new strawberry ($Fragaria{\times}ananassa$ Duch.) cultivar, which was released by National Institute of Highland Agriculture, RDA, in 2008. In 2004, this cultivar was originated from a cross between 'Samahberi' and 'Elan' and showed excellent ever-bearing characteristics including continuous flowering habit, high yield under long-day and high temperature condition. It was named 'Saebong No.2' after examining its characteristic and productivity in summer culture from 2006 to 2007. Again, after two regional adaptability tests in 2008, 'Gangha' was selected as an elite cultivar. The general characteristics of 'Gangha' include semi-spreading type, middle vigor, elliptic leaf, and long cluster in growth. The fruit shape is oval and fruit color is red. Fruit quality had 1ow activity, hardness but high soluble solids contents. This average fruit weight of 'Gangha' is about 12.0 g and the marketable yield is relatively high because of more flower cluster appearance. It is resistant against powdery mildew, anthracnose, and fusarium wilt. 'Gangha' is suitable for summer culture using domestic consumption because it is shows continuous flowering habit, high yield, and fruit quality under long-day and high temperature condition.

Interpretation Method of Eco-Cultural Resources from the Perspective of Landscape Ecology in Jeju Olle Trail (제주 올레길 생태문화자원 경관생태학적 해석기법 연구)

  • Hur, Myung-Jin;Han, Bong-Ho;Park, Seok-Cheol
    • Journal of the Korean Institute of Landscape Architecture
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    • v.49 no.2
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    • pp.128-140
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    • 2021
  • This study applied the theory of Landscape Ecology to representative resources of Jeju Olle-gil, which is a representative subject of walking tourism, to identify ecological characteristics and to establish a technique for landscape ecological analysis of Olle-gil resources. Jeju Olle Trail type based on the biotope type, major land use, vegetation status around Olle Trail and roads were divided into 12 types. Based on the type of ecological tourism resource classification, the Jeju Olle-gil walking tourism resource classification was divided into seven types of natural resources and seven types of humanities resources, and each resource was characterized by Geotope, Biotope, and Anthropopope, just like the landscape ecology system. Geotope resources are strong in landscape characteristics such as coast and beach, rocks, bedrocks, waterfalls, geology and Jusangjeolli Cliff, Oreum and craters, water resources, and landscape viewpoints. The Biotope resources showed strong ecological characteristics due to large tree and protected tree, Gotjawal, forest road and vegetation communities, biological habitat, vegetation landscape view point. Antropotope include Culture of Jeju Haenyeo and traditional culture, potting and lighthouses, experience facilities, temples and churches, military and beacon facilities, other historical and cultural facilities, and cultural landscape views. Jeju Olle Trail The representative resources for each type of Jeju Olle Trail are coastal, Oreum, Gotjawal, field and Stonewall Fencing farming land, Jeju Village and Stone wall of Jeju. In order to learn about the components and various functions of the resources representing the Olle Trail's ecological culture, the landscape ecological technique was interpreted. Looking at the ecological and cultural characteristics of coastal, the coast includes black basalt rocks, coastal vegetation, coastal grasslands, coastal rock vegetation, winter migratory birds and Jeju haenyeo. Oreum is a unique volcanic topography, which includes circular and oval mountain bodies, oreum vegetation, crater wetlands, the origin and legend of the name of Oreum, the legend of the name of Oreum, the culture of grazing horses, the use of military purposes, the object of folk belief, and the view from the summit. Gotjawal features rocky bumps, unique microclimate formation, Gotjawal vegetation, geographical names, the culture of charcoal being baked in the past, and bizarre shapes of trees and vines. Field walls include the structure and shape of field walls, field cultivation crops, field wall habitats, Jeju agricultural culture, and field walls. The village includes a stone wall and roof structure built from basalt, a pavilion at the entrance of the village, a yard and garden inside the house, a view of the lives of local people, and an alleyway view. These resources have slowly changed with the long lives of humans, and are now unique to Jeju Island. By providing contents specialized for each type of Olle Trail, tourists who walk on Olle will be able to experience the Olle Trail in depth as they learn the story of the resources, and will be able to increase the sustainable use and satisfaction of Jeju Olle Trail users.