Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Mycelial growth properties of an Amanita javanica strain under various culture conditions

노란달걀버섯 균주의 여러 환경 조건에 따른 균사 생장 특성

  • Kang, Jung-A (Department of Microbiology, College of Natural Science, Dankook University) ;
  • Ka, Kang-Hyeon (Special Forest Products Division, National Institute of Forest Science) ;
  • Kim, Jun Young (Department of Microbiology, College of Natural Science, Dankook University) ;
  • Yoon, Seong-Tak (Department of Crop Science and Biotechnology, Dankook University) ;
  • Kim, Seong Hwan (Department of Microbiology, College of Natural Science, Dankook University)
  • 강정아 (단국대학교 미생물학과) ;
  • 가강현 (국립산림과학원 산림소득자원연구과) ;
  • 김준영 (단국대학교 미생물학과) ;
  • 윤성탁 (단국대학교 식량생명공학과) ;
  • 김성환 (단국대학교 미생물학과)
  • Received : 2019.12.01
  • Accepted : 2019.12.23
  • Published : 2019.12.31
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Abstract

The edible ectomycorrhizal mushroom Amanita javanica is a valuable species protected by forest law in Korea. However, basic characterization data on its use as an important forest resource has been limited. This study was performed to determine mycelia growth characteristics of the domestically isolated Amanita javanica strain NIFoS 1267 on potato dextrose agar media under diverse culture conditions. Physical factors temperature, pH, and light, as well as chemical factors salts, heavy metals, and pesticides were examined for their effects on the growth of the mushroom strain. The mycelia of A. javanica strain exhibited optimal growth when cultured in dark at 30℃ in media with a pH of 5-6. Normal levels of growth were observed in media containing up to 2% saline. At a heavy metal ion content of 50 ppm, mycelial growth was not affected by arsenic ion but was affected by cadmium and lead ions. In the tests performed with two pesticides used in Korean forests, the growth of the mushroom strain was not affected by the presence of abamectin, but was inhibited in media containing acetamiprid, emamectin benzoate, or thiacloprid. These results are expected to facilitate artificial cultivation of A. javanica as a new commercial product.

노란달걀버섯은(Amanita javanica) 국내에서 산림법으로 보호받고 있는 식용 가능한 외생균근성 버섯이나, 중요한 산림자원으로서 활용하기 위한 기초적인 특성 연구에 대한 자료는 부족한 실정이다. 이에 본 연구는 국내 채집 야생버섯 자실체로부터 분리한 노란달걀버섯 NIFoS 1267 균주를 이용하여 PDA 배지 상에서 물리적 요인(온도, pH, 광)과 화학적인 요인(염분, 중금속, 농약)에 따른 균사생장 특성을 조사하였다. 최적의 물리적 환경은 온도가 30℃, pH가 5-6, 암조건으로 배양이었을 때 노란달걀버섯 균주의 균사생장이 가장 좋은 것으로 나타났다. 화학적 요인으로서 염분은 2.0% 농도 조건까지 버섯균주의 균사 생장이 가능하였다. 50 ppm 농도의 중금속 이온 환경에서 비소(As) 이온은 노란달걀버섯 균주의 균사생장에 영향을 주지 않았으나 카드뮴(Cd)와 납(Pb) 이온은 균사 생장이 불가능하게 하였다. 국내 산림에 사용되고 있는 2가지 농약의 경우, Abamectin 첨가 배지에서는 노란달걀버섯 균주의 균사 생장에 영향이 없었으나 Acetamiprid, Emamectin benzoate, Thiacloprid가 첨가된 배지 환경에서는 균사 생장이 모두 다 저해 되었다. 본 연구 결과는 향후 상업적 생산을 위한 새로운 자원으로서 노란달걀버섯의 인공재배 연구에 도움이 될 것으로 기대된다.

Keywords

References

  1. An GH, Han JG, Cho JH. 2019. Antioxidant activities and $\beta$-glucan contents of wild mushrooms in Korea. J Mushrooms 17: 144-151.
  2. Boa E. 2004. Wild edible fungi: A global overview of their use and importance to people. Food and Agriculture Organization of the United Nations, Rome.
  3. den Bakker HC, Zuccarello GC, Kuyper THW, Noordeloos ME. 2004. Evolution and host specificity in the ectomycorrhizal genus Leccinum. New Phytol 163: 201-215. https://doi.org/10.1111/j.1469-8137.2004.01090.x
  4. Eger G. 1962. Untersuchungen zur Fruchtkorperbildung des Kulturchampignons. Mushroom Science 5: 314-320.
  5. Esser K, Meinhardt F. 1971. A common genetic control of dikaryotic and monokaryotic fruiting in basidiomycetes Agrocybe aserita. Mycologia 62: 136.
  6. Han YH, Ham JH. 1999. Optimization of culture condition for enhanced mycelial growth of Amanita hemibapha subsp. hemibapha DGUM 28001. Dongguk Journal: Natural Sciences 38: 273-286.
  7. Jang MJ, Lee YH, Cho YK, Koo HM, Oh TS. 2015. Growth properties of Neolentinula lepideus according to the light environment. J Mushrooms 13: 125-128. https://doi.org/10.14480/JM.2015.13.2.125
  8. Jeon SM, Ka KH. 2015. Cultural characteristics of Korean ectomycorrhizal fungi. Kor J Mycol 43: 1-12. https://doi.org/10.4489/KJM.2015.43.1.1
  9. Ka KH, Jeon SM, Ryoo R, Bak WC, Kang JA, Kim MG, Jeon HS, Jeong YS. 2014. Basic culture characteristics of ectomycorrhizal mushrooms. Korea Forest Research Institute, Research Report 575.
  10. Ka KH, Jeon SM, Ryoo R, Ryu SH, Kim MK, Bak WC, Park JW, Koo CD, Eom AH. 2011. Management of genetic resource of forest microorganisms. Korea Forest Research Institute, Research Report 434.
  11. Kang JA, Ka KH, Kim JY, Kim SH. 2018. Mycelial growth properties of domestically collected ectomycorrhizal Tricholoma mushrooms in various culture conditions. Kor J Mycol 46: 271-280. https://doi.org/10.4489/KJM.20180037
  12. Kim SH, Uzunovic A, Breuil C. 1999. Rapid detection of Ophiostoma piceae and O. quercus in stained wood using PCR. Appl Environ Microbiol 65: 287-90. https://doi.org/10.1128/AEM.65.1.287-290.1999
  13. Kwon HW, Yoon JH, Kim SH, Hong SB, Cheon Y, Ko SJ. 2007. Detection of extracellular enzyme activities in various Fusarium spp. Mycobiology 35: 162-165 https://doi.org/10.4489/MYCO.2007.35.3.162
  14. Oda T, Tanaka C, Tsuda M. 1999. Molecular phylogeny of Japanese Amanita species based on nucleotide sequences of the internal transcribed spacer region of nuclear ribosomal DNA. Mycoscience 40: 57-64. https://doi.org/10.1007/BF02465674
  15. Sanmee R, Dell B, Lumyong P, Izumori K, Lumyong S. 2003. Nutritive value of popular wild edible mushrooms from northern Thailand. Food Chem 82: 527-532. https://doi.org/10.1016/S0308-8146(02)00595-2
  16. Sou HD, Hur TC, Jung SC, Joo SH, Park H. 2011. Analysis of environmental characteristics in habitat of Amanita hemibapha. Kor J Mycol 39: 164-170. https://doi.org/10.4489/KJM.2010.39.3.164
  17. Sou HD, Hur TC, Joo SH. 2009. Morphological and physiological characteristics of Amanita hemibapha subsp. hemibapha (Berk. & Broome) Sacc. Kor J Mycol 37: 41-48. https://doi.org/10.4489/KJM.2009.37.1.041
  18. Sylvia DM, Fuhrmann JJ, Hartel PG, Zuberer DA. 2005. Principles and applications of soil microbiology (2nd Edition). Pearson Prentice Hall, New Jersey, USA.
  19. Walter M, Frampton CM, Boyd-Wilson KSH, Harris-Virgin P, Waipara W. 2007. Agrichemical impact on growth and survival of non-target apple phyllosphere microorganisms. Can J Microbiol 53: 45-55. https://doi.org/10.1139/w06-093
  20. Wisplinghoff H, Bischoff T, Tallent SM, Serifert H, Wenzel RP, Edmond MB. 2004. Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis 39: 309-331. https://doi.org/10.1086/421946
  21. Zhang B, Bai Z, Hoefel D, Tang L, Wang X, Li B, Li Z, Zhuang G. 2009. The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere. Sci Total Environ 407: 1915-1922. https://doi.org/10.1016/j.scitotenv.2008.11.049